Leaf volatiles and stem bark exudates of two swietenia species

composition and bioactivity

| Posted in: Science

Journal name: World Journal of Pharmaceutical Research
Original article title: Leaf volatiles and stem bark exudates of two swietenia species
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Subtitle: composition and bioactivity

Original source:

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Author:

Ola Mohamed Mousa, Marwa Yousry Moustafa Issa, Hesham Ibrahim El-Askaryand Soheir Mohamed El Zalabani


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Leaf volatiles and stem bark exudates of two swietenia species

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Copyright (license): WJPR: All rights reserved


Summary of article contents:

Introduction

The study conducted by Mousa et al. focuses on the hydrodistilled leaf volatiles and stem bark exudates of two Swietenia species, namely Swietenia mahogani and Swietenia macrophylla, grown in Egypt. Both species belong to the Meliaceae family and are recognized for their wood quality and potential medicinal properties. Given the scarcity of research on their leaf volatiles and stem bark exudates, this investigation aimed to evaluate their chemical compositions and assess their antimicrobial and antihyperglycemic activities. Various analytical methods, including chromatographic analyses, were employed to explore the chemical profiles and to determine the efficacy of these plant-derived substances against specific microorganisms.

Antimicrobial Activity of Leaf Volatiles

One significant finding of the study is the antimicrobial activity exhibited by the leaf volatiles of the two Swietenia species. The hydrodistilled leaf volatiles demonstrated effectiveness against a range of Gram-positive bacteria, notably Staphylococcus aureus and Mycobacterium phlei, with minimum inhibitory concentrations (MICs) ranging from 6 to 12.5 µl/ml. In contrast, there was no observable effect on Gram-negative bacteria or the yeast Candida albicans. The study highlights the potential of these volatiles in contributing to microbial resistance, emphasizing the possibility of utilizing them as natural antimicrobial agents in pharmaceutical applications.

Chemical Composition of Leaf Volatiles

In the analysis of the leaf volatiles, the study revealed a rich composition dominated by sesquiterpenoids, particularly hydrocarbons, comprising 75.51% in S. mahogani and 80.95% in S. macrophylla. Significant components identified included trans-caryophyllene, which constituted 33.89% in S. mahogani, while α-humulene was found to be the predominant compound (39.64%) in S. macrophylla. The presence of these compounds suggests possible ecological functions such as pest attraction, and the variability in the chemical composition offers chemotaxonomical criteria for differentiating between the two species. The study emphasizes that climatic and geographical factors can significantly influence these compositions.

Antihyperglycemic Activity of Stem Bark Exudates

Another impactful aspect investigated in the study was the long-term antihyperglycemic activity of the stem bark exudates. In diabetic rats induced by Alloxan, treatment with the aqueous solutions of the stem bark exudates from both species resulted in a notable decline in blood glucose levels. Specifically, the aqueous extract from S. mahogani exhibited a higher potency (69% reduction) than that from S. macrophylla (62% reduction), compared to the standard drug, Metformin. This finding underlines the potential therapeutic applications of these plant extracts in managing hyperglycemia, thus promoting further research into their use in diabetes treatment.

Conclusion

The findings from this study provide valuable insights into the chemical and biological properties of the leaf volatiles and stem bark exudates of Swietenia mahogani and Swietenia macrophylla. The demonstrated antimicrobial activity against specific bacteria, combined with the antihyperglycemic effects observed in diabetic rat models, suggests significant medicinal potential. The distinct chemical compositions of the volatiles also offer chemotaxonomical insights that may aid in the identification and differentiation among plant species. Overall, this research supports the notion that local cultivars of Swietenia can be harnessed for their therapeutic properties, encouraging greater exploration and utilization in the field of pharmaceutical applications.

FAQ section (important questions/answers):

What are the two Swietenia species studied in the research?

The study focuses on Swietenia mahogani (L.) Jacq. and Swietenia macrophylla King, both grown in Egypt.

What techniques were used to analyze the chemical composition?

Chemical composition was determined using chromatographic analyses such as PC, GLC, and GC/MS.

What was the main bioactivity tested in the study?

Antimicrobial potential and long-term antihyperglycemic activity of leaf volatiles and stem bark exudates were evaluated.

What dominated the hydrodistilled leaf volatiles from both species?

The hydrodistilled leaf volatiles were predominantly composed of sesquiterpenoids, especially hydrocarbons.

How were the antimicrobial activities assessed in the research?

Antimicrobial activities were evaluated using the agar diffusion method and Minimum Inhibitory Concentrations were determined.

What effect did the stem bark exudates have on blood glucose levels?

The stem bark exudates significantly reduced blood glucose levels in Alloxan-diabetic rats.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Leaf volatiles and stem bark exudates of two swietenia species”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Activity:
In the context of the study, 'Activity' refers to the biological effects exhibited by the leaf volatiles and stem bark exudates of the Swietenia species. Both antimicrobial and antihyperglycemic activities were assessed, showcasing the potential of these plant products for medicinal applications, particularly in controlling bacterial growth and managing blood glucose levels in diabetic models.

2) Table:
'Table' denotes structured presentations of data within the research paper. Tables serve to organize findings, such as compositions and antimicrobial activities, in a clear and concise manner, allowing for easy comparison. They effectively summarize quantitative data, aiding in comprehending the relationships and differences between the studied samples.

3) Species:
'Species' identifies the biological classification of organisms, crucial in the paper as it discusses Swietenia mahogani and Swietenia macrophylla. Understanding the differences between these closely related tree species is vital for identifying their unique properties and potential uses in pharmacology, ecology, and industry, guiding both research and conservation efforts.

4) Egypt:
'Egypt' contextualizes the geographical location where the Swietenia species were cultivated and studied. The country's unique flora, combined with its historical significance in botany and pharmacology, emphasizes Egypt's role in understanding the biodiversity and potential utility of local plant materials in developing medicinal products.

5) Water:
'Water' is a fundamental element in the study, serving as a solvent for the preparation of exudates and for hydrodistillation. It is essential for the solubility of various compounds, facilitating the extraction of bioactive substances from the plant materials, thereby influencing the overall efficacy and applicability of the resulting extracts.

6) Relative:
'Relative' is used to compare various parameters, such as the composition and activity of the samples. It assists in establishing relationships between different components, such as how one species may possess more of a certain compound compared to another, which is vital for classification and understanding the potential uses of the plants.

7) Mineral:
'Mineral' refers to the inorganic constituents analyzed in the ash of the exudates, providing insights into the nutrient profile of Swietenia species. Analyzing mineral content helps assess the overall health benefits and medicinal potential of these botanicals, contributing to understanding their roles in traditional and modern medicine.

8) Animal:
'Animal' pertains to the model organisms used for evaluating the antihyperglycemic effects of the stem bark exudates. This study employed Sprague Dawley rats for experimentation, demonstrating the biological relevance of Swietenia extracts and their effects on diabetes, crucial for validating potential therapeutic approaches.

9) Blood:
'Blood' represents a critical biological fluid whose glucose levels were measured to evaluate the antihyperglycemic effects of the studied extracts. Monitoring blood glucose is essential in diabetes research, providing a direct measure of the efficacy of treatments being tested on diabetic models, emphasizing the therapeutic relevance of the findings.

10) King:
'King' refers to the specific nomenclature of the species Swietenia macrophylla, highlighting the importance of accurate scientific naming in taxonomy. This classification aids in the understanding of plant relationships, medicinal properties, and conservation status, playing a vital role in both botanical and ecological studies.

11) Drug:
'Drug' indicates the therapeutic agents tested against microbial cultures and used in diabetic treatment comparisons. The exploration of natural products like those derived from Swietenia as potential drugs reflects ongoing research into developing effective, safe, and accessible treatments in modern pharmacotherapy, especially for antimicrobial and metabolic conditions.

12) Sugar:
'Sugar' is significant in the analysis of the carbohydrate composition of the stem bark exudates. The presence and relative proportions of sugars such as galactose and xylose contribute to understanding the nutritional and medicinal profiles of these plants, serving to inform possible dietary and therapeutic applications.

13) Antibiotic (Antibacterial):
'Antibacterial' describes the ability of the leaf volatiles to inhibit bacterial growth. This property is crucial in the study as it establishes the potential of Swietenia species as sources of natural antimicrobial agents, thereby contributing to drug development efforts against antibiotic-resistant pathogens and enhancing therapeutic options.

14) Heating:
'Heating' refers to the method used in hydrolyzing exudates for sugar analysis. This process helps in extracting and characterizing polysaccharides, emphasizing the importance of applying controlled thermal conditions in laboratory procedures to ensure accurate results essential for understanding the chemical composition of plant materials.

15) Aureus:
'Aureus' denotes Staphylococcus aureus, a critical pathogen tested in the study to assess antimicrobial activity. The choice of this bacterium is significant as it is known for its role in human infections, making it an important target for evaluating the efficacy of natural extracts in combating common bacterial threats.

16) Insect:
'Insect' pertains to the potential use of Swietenia species as natural insect antifeedants. This aspect highlights the ecological implications of the study, as research into plant-derived insect deterrents supports sustainable agricultural practices while reducing reliance on synthetic pesticides and promoting environmental health.

17) Study (Studying):
'Study' represents the overarching research effort conducted to analyze the leaf volatiles and stem bark exudates. It emphasizes the systematic inquiry into the composition, antimicrobial properties, and antihyperglycemic effects of the plants, contributing to the body of knowledge necessary for further pharmacological advancements and biodiversity conservation.

18) Rich (Ṛch):
'Rich' characterizes the abundance of specific compounds or nutrients within the studied materials. The term indicates a high concentration of valuable bioactive substances in the leaf volatiles and exudates, underscoring their potential utility in medicinal applications and highlighting their importance in pharmaceutical and nutraceutical development.

19) Incision:
'Incision' refers to the method used to extract the stem bark exudates from the Swietenia trees. This technique is crucial in ensuring adequate collection of the plant material without causing detrimental harm to the trees, thereby promoting sustainable harvesting practices while facilitating research into their beneficial properties.

20) Forest:
'Forest' relates to the natural habitat of Swietenia species, emphasizing their role in biodiversity. Understanding the ecological context of these trees contributes to conservation efforts and biodiversity studies, highlighting their significance not only in pharmaceutical applications but also in maintaining healthy ecosystems.

21) Nature:
'Nature' indicates the inherent qualities and characteristics of the studied substances. The term highlights the relationship between biological organisms and their environment, underscoring the importance of natural products in developing sustainable solutions for health-related challenges and promoting the understanding of ecological interdependencies.

22) Street:
'Street' denotes the specific location of the researchers' institution in Cairo, Egypt. It contextualizes the study, indicating where the research was conducted and reflecting on the infrastructure supporting scientific endeavors. The physical setting can also influence botanical studies by determining availability of local plant species.

23) Male (Mālē):
'Male' specifies the gender of the rats used in the study for evaluating antihyperglycemic activity. The choice of male subjects is critical in controlled experiments, contributing to standardization practices in biomedical research, ensuring that results are comparable and can be generalized within the context of diabetes research.

24) Biodegradable:
'Biodegradable' describes the potential environmental advantage of using natural extracts instead of synthetic chemicals. As the study explores plant-derived substances, their biodegradable nature supports sustainability in pest and disease control, aligning with upcoming trends aimed at reducing ecological footprints and promoting environmentally-friendly agricultural methods.

25) Pharmacology:
'Pharmacology' is the field encompassing the study of drug action, critical in evaluating the therapeutic potential of Swietenia extracts. Understanding the pharmacological properties of these natural products guides drug development, ensuring that active components are effectively harnessed for medicinal purposes, maximizing their benefits to human health.

26) Performance:
'Performance' relates to the effectiveness and efficiency of the tested extracts in achieving antimicrobial and antihyperglycemic effects. Evaluating performance measures the outcomes of treatments in the context of medicinal applications, serving to inform further research and practical applications in healthcare and pharmacotherapy.

27) Discussion:
'Discussion' reflects the section of the research paper that interprets results, linking findings to broader contexts. This segment engages with existing literature, explores implications of results, and provides insights into the significance of the study, guiding future research directions and practical applications in science and medicine.

28) Similarity:
'Similarity' refers to the evaluation of related characteristics between the two studied species, Swietenia mahogani and Swietenia macrophylla. Establishing similarities helps in taxonomical classification and understanding evolutionary relationships, as well as in predicting shared chemical properties and potential uses in pharmacological applications.

29) Harvesting (Harvest):
'Harvesting' indicates the collection of plant materials, a critical aspect in the study to assess availability and sustainability of the Swietenia species. Responsible harvesting practices ensure ecological balance while promoting conservation efforts, facilitating the continuous exploration of plant-based therapies in modern medicine.

30) Toxicity:
'Toxicity' examines the safety of the studied substances, an essential factor in evaluating their medicinal potential. Understanding the toxicity levels helps to determine the suitability of extracts for therapeutic use, guiding research towards developing safe and effective natural treatments with minimal adverse effects.

31) Science (Scientific):
'Science' represents the systematic study of the natural world through observation and experimentation. This research contributes to the field of science by expanding knowledge on the pharmacological properties of Swietenia species, facilitating advancements in agents for treating health issues, reflecting the ongoing pursuit of scientific understanding.

32) Medium:
'Medium' in the context of this study typically refers to the growth environment used for microbial cultures during antimicrobial testing. The effectiveness of a culture medium influences microbial growth and efficacy testing results, making it a critical element in microbiological assessments of the bioactivity of the extracts.

33) Trade:
'Trade' highlights the economic aspect of Swietenia species as valuable commodities in the timber and pharmaceutical industries. Understanding the implications of trade in these plants facilitates assessments of their conservation and sustainable use, guiding policies that balance economic interests with ecological sustainability.

34) House:
'House' refers to the animal house where Sprague Dawley rats were kept for the experiments. Proper housing conditions are critical for maintaining the health and well-being of laboratory animals, ensuring reliable results in pharmacological studies while adhering to ethical research standards.

35) Fainting (Fainted):
'Faint' describes the subtle responses observed in the chemical tests of the exudates, indicating minimal presence of certain constituents. This term emphasizes the sensitivity and precision required in analytical techniques, ensuring accurate interpretation of results while highlighting the need for rigorous testing methodologies in research.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Leaf volatiles and stem bark exudates of two swietenia species’. Further sources in the context of Science might help you critically compare this page with similair documents:

Swietenia macrophylla, Chemical composition, Organoleptic characteristics, Antimicrobial activity, Moisture content, Minimum inhibitory concentration, Physico-chemical characteristics, Anti-microbial activity, Secondary metabolite, Staphylococcus aureus, Antihyperglycemic activity, Retention time, Gram positive bacteria, Structural study, Organoleptic features, Volatile oil, Total ash content, Chromatographic analysis, Anti-hyperglycemic activity, Antimicrobial potential, Blood glucose level, Mechanisms of action, Growth inhibitory activity, Hydrodistillation, Plant Extract, Mineral Composition, Structure-activity relationship, Agar diffusion method, Diameter of zone of inhibition, Alloxan-diabetic rats, Biological investigation, Phyto-chemical properties, Grams-positive bacteria, Comparative investigation, Gas-Liquid chromatography, Chromatographic profile, Chemical test, Atomic absorption spectrometry, Analytical parameter, Plant product, Diabetic rat.

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