GC-MS identification of bioactive compounds in Glycosmis pentaphylla.

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Journal name: World Journal of Pharmaceutical Research
Original article title: Identification of some bioactive constituents of glycosmis pentaphylla retz. dc. using gc-ms
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Author:

M. Sivakumar, D.Chamundeeswari, Nalini Ganesan and Hannah. R .Vasanthi


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World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Identification of some bioactive constituents of glycosmis pentaphylla retz. dc. using gc-ms

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Copyright (license): WJPR: All rights reserved


Summary of article contents:

Introduction

Glycosmis pentaphylla, known as "Anam" in Tamil, is a significant medicinal plant belonging to the Rutaceae family. Traditionally, its leaves, fruits, and roots have been utilized to treat various ailments, including fever, hepatopathy, wounds, eczema, rheumatism, jaundice, and skin diseases. Although numerous bioactive compounds have been identified in different parts of the plant, this study focused on the unexplored constituents present in the roots using Gas Chromatography-Mass Spectrometry (GC-MS) analysis.

Identification of Bioactive Compounds

The study successfully identified 25 chemical constituents from the ethanolic extract of the plant's roots, out of which six compounds were reported for the first time. Notable among these is 7H-Furo(3,2-G)(1)Benzopyran-7-one, also referred to as marmesin, which constitutes approximately 10.96% of the extract. This novel finding adds significant value to the understanding of this plant's pharmacological potential, considering marmesin's classification as a dihydro furocoumarin derivative with possible health benefits.

Methodology and Extraction Process

To conduct the analysis, the roots of Glycosmis pentaphylla were collected, dried, pulverized, and then successively extracted using various solvents, ultimately producing an ethanolic extract. A sophisticated GC-MS-QP 2010 instrument was employed to analyze the extract, allowing for the identification of compounds based on their mass spectra compared to a comprehensive database. This methodological rigor ensured that a diverse range of chemical constituents was accurately cataloged.

Importance of Phytochemicals

The identified phytochemicals from Glycosmis pentaphylla justify its longstanding traditional use in various medicinal applications. Compounds like Gama-Sitosterol, 5-hydroxypipecolic acid, and others were also highlighted for their respective bioactive roles. The presence of these compounds underlines the plant's potential as a source of various therapeutic agents, supporting its validation in traditional medicine practices and warranting further pharmacological investigations.

Conclusion

The study highlights the rich biochemical profile of Glycosmis pentaphylla, revealing several previously unidentified bioactive compounds through GC-MS analysis. The findings affirm the plant's importance in traditional medicine and its promising potential for future phytochemical research. Recommendations for advancing studies include isolating individual compounds and exploring their biological activities to fully understand their therapeutic possibilities, thus further establishing the plant's role in pharmacology and alternative medicine.

FAQ section (important questions/answers):

What is Glycosmis pentaphylla commonly known as?

Glycosmis pentaphylla is commonly referred to as 'Anam' in Tamil and has various names in different languages, including Vananimbuka in Sanskrit and Ban-nimbu in Hindi.

What are the traditional medicinal uses of Glycosmis pentaphylla?

The plant is traditionally used for treating fever, hepatopathy, skin diseases, rheumatism, jaundice, and helminthiasis, among other ailments.

What was the objective of the study on Glycosmis pentaphylla?

The study aimed to identify new chemical constituents in the ethanolic extract of Glycosmis pentaphylla's roots through GC-MS analysis.

How many chemical constituents were identified in the study?

The study identified twenty-five chemical constituents from the ethanolic extract, six of which were reported for the first time.

What techniques were used to analyze the extracts of Glycosmis pentaphylla?

Gas chromatography-mass spectrometry (GC-MS) was employed to analyze the ethanolic root extract of Glycosmis pentaphylla.

What did the study conclude about Glycosmis pentaphylla's bioactive compounds?

The study concluded that Glycosmis pentaphylla contains various bioactive compounds justifying its traditional medicinal use, suggesting further investigation into its pharmacological properties.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “GC-MS identification of bioactive compounds in Glycosmis pentaphylla.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) India:
India is a country in South Asia where Glycosmis pentaphylla is commonly found. Its traditional uses in local medicine highlight the cultural significance of flora in health practices. The plant's application in treating various ailments reflects India's rich biodiversity and traditional knowledge of medicinal plants.

2) Study (Studying):
The term study denotes a systematic investigation focused on identifying chemical constituents in Glycosmis pentaphylla. It implies a research approach aimed at understanding the plant's properties, emphasizing the importance of scientific methods in validating traditional uses of medicinal plants in contemporary medicinal applications.

3) Phytochemical:
Phytochemicals are bioactive compounds derived from plants, specifically highlighting their medicinal properties. In the context of Glycosmis pentaphylla, the study identifies various phytochemicals that may contribute to its therapeutic effects, underscoring the relevance of plant chemistry in developing herbal medicines and understanding their biological activities.

4) Chennai:
Chennai is a major city in India, home to the Faculty of Pharmacy at Sri Ramachandra University, where the study was conducted. The city represents a hub for pharmaceutical research and education, emphasizing the role of urban centers in advancing scientific knowledge, particularly in traditional herbal medicine.

5) Tamil (Tamiḻ):
Tamil refers to the language spoken predominantly in the Indian state of Tamil Nadu and in parts of Sri Lanka. The plant Glycosmis pentaphylla, known as 'Anam' in Tamil, showcases the linguistic diversity associated with regional medicinal plants and highlights the indigenous knowledge tied to traditional healing practices.

6) Gama (Gāma):
Gama, or more specifically Gamma-Sitosterol, is a phytochemical identified in the study. This compound plays a role in human health, including potential cholesterol-lowering effects. Its presence reinforces the importance of diverse phytochemicals found in Glycosmis pentaphylla that may be linked to various health benefits.

7) Inflammation:
Inflammation signifies the body's response to injury or infection, often associated with various diseases. Traditional uses of Glycosmis pentaphylla in treating inflammation demonstrate its potential therapeutic applications. The study's focus on phytochemicals suggests possible anti-inflammatory properties that merit further examination within modern pharmacology.

8) Tamilnadu (Tamil-nadu, Tamilnāḍu):
Tamil Nadu is the Indian state where Glycosmis pentaphylla is prevalent. The state's unique climate and biodiversity support a rich variety of medicinal plants. Understanding the local flora informs both traditional medicine practices and scientific research, enhancing knowledge of medicinal plants and their therapeutic potentials.

9) Medicine:
Medicine encompasses the science and practice of diagnosing, treating, and preventing illness. The mention of Glycosmis pentaphylla within the study reinforces its significance in traditional and contemporary settings, highlighting the plant's applications in addressing health concerns through both herbal and pharmacological avenues.

10) Disease:
Disease refers to an abnormal condition impacting normal bodily functions, often treated with medicinal compounds. The study of Glycosmis pentaphylla reveals its potential to combat diseases traditionally treated with herbal remedies, demonstrating the importance of understanding plant-based treatments in the arena of medicinal discovery.

11) Powder:
Powder refers to the processed form of the root of Glycosmis pentaphylla, which was shade-dried and pulverized for extraction. The preparation of powders is crucial in herbal medicine for enhancing solvent extraction processes, thereby enabling researchers to isolate and analyze the active chemical constituents effectively.

12) Table:
The term table here refers to a structured format presenting the identified phytochemical constituents from the GC-MS analysis. Tables play a vital role in summarizing research data, making complex information accessible and aiding in the clarity of scientific communication regarding the constituents of Glycosmis pentaphylla.

13) Fever:
Fever is a common symptom indicative of an underlying infection or disease. Glycosmis pentaphylla is traditionally used in treating fever, reflecting its historical significance in herbal medicine. The study's exploration of bioactive compounds may contribute to understanding the plant's efficacy against fever-related ailments.

14) Nadu (Nāḍu):
Nadu is part of 'Tamil Nadu,' the state in which Glycosmis pentaphylla thrives. The regional context emphasizes the local heritage of medicinal plants. Understanding its use in traditional medicine can guide modern scientific research and facilitate the preservation of indigenous knowledge related to herbal remedies.

15) Beta (Bēṭa, Beṭa):
The term beta relates to beta-sitosterol, a sterol identified in the study. Beta-sitosterol is known for several health benefits, including cholesterol management and anti-inflammatory properties. Its detection in Glycosmis pentaphylla reinforces the plant's medicinal potential and supports its historical usage in traditional remedies.

16) Pharmacological:
Pharmacological refers to the study of drug action and effects on biological systems. The research on Glycosmis pentaphylla aims to uncover pharmacological interactions of its phytochemical constituents. This knowledge is vital for validating traditional medicinal uses and advancing the integration of herbal medicines in modern pharmacology.

17) Puducherry:
Puducherry is a Union Territory in India with historical ties to Tamil culture. The mention of Puducherry indicates the geographical distribution of Glycosmis pentaphylla, emphasizing the importance of studying regional plants. It also highlights the influence of locale in the application and research of traditional herbal remedies.

18) Discussion:
Discussion involves an analytical examination of the study’s findings related to the identification of the phytochemical constituents of Glycosmis pentaphylla. This section is crucial for interpreting results, connecting them with existing literature, and proposing future research directions, particularly regarding medicinal benefits and applications.

19) Science (Scientific):
Scientific pertains to the methodologies and approaches used in the study of Glycosmis pentaphylla. It underscores the rigor and systematic nature of the research, which aims to bridge traditional knowledge with modern scientific techniques necessary for understanding the plant's composition and therapeutic applications.

20) Tirupathi:
Tirupathi is a city known for its rich biodiversity and is the location where the Glycosmis pentaphylla roots were collected. Its geographic significance contributes to the study by providing access to indigenous plant species and reflects the regional connection to traditional medicinal practices.

21) Tirumala (Thirumala):
Tirumala is a hill town near Tirupathi famous for its temples and biodiversity. The recruitment of Glycosmis pentaphylla from this region signifies the traditional significance of local plants, and exploration of such flora can help in understanding the link between cultural heritage and medicinal resource availability.

22) Toxicity:
Toxicity refers to the harmful effects of substances on living organisms. In the context of Glycosmis pentaphylla, evaluating the toxicity profile is crucial for safety assessments in herbal medicine. The study emphasizes the need for comprehensive research to ensure the safety and efficacy of identified bioactive compounds.

23) Activity:
Activity in this context pertains to the biological effects of the identified phytochemicals. The study's exploratory nature seeks to link the presence of these compounds to their potential therapeutic effects, emphasizing the need for biological testing to validate the medicinal properties traditionally attributed to Glycosmis pentaphylla.

24) Language:
Language refers to the means of communication within a given culture. Tamil is one of the languages connecting the people of South India to their traditional medicinal practices. Understanding language helps preserve cultural heritage and enhances communication regarding the medicinal properties of local flora such as Glycosmis pentaphylla.

25) Vitiation (Vitiated):
Vitiated refers to a disturbed state of balance in the body, commonly used in traditional healing contexts. Glycosmis pentaphylla is utilized in treating vitiated conditions of the three doshas—vata, pitta, and kapha—which are foundational concepts in Ayurvedic medicine, reflecting the plant's role in holistic health practices.

26) Sanskrit:
Sanskrit is an ancient language of India, rich in historical texts relating to medicine. Terms derived from Sanskrit are often used in Ayurveda, the traditional system of medicine. This highlights the cultural and historical framework that supports the understanding of medicinal plants like Glycosmis pentaphylla.

27) Kannada (Kannaḍa):
Kannada is a regional language spoken in the Indian state of Karnataka, where traditional knowledge of medicinal plants also thrives. The mention of Kannada reflects the linguistic diversity prevalent in India and emphasizes the need to recognize various cultural practices associated with herbal medicine across different languages.

28) Nalini (Nalinī, Nālīnī, Nālinī, Naḷiṉi, Nāḷiṉi):
Nalini refers to one of the authors of the study. The involvement of researchers like Nalini underscores the collaborative effort in scientific exploration, providing a diverse range of skills and perspectives essential for thorough investigations into traditional medicine and plant biochemistry.

29) Andhra (Āndhra, Amdhra):
Andhra refers to Andhra Pradesh, the state in which Glycosmis pentaphylla was collected. This highlights the geographical significance of the study, contributing to the understanding of local medicinal plants. Regional studies enhance the knowledge of flora and its application in traditional medicine.

30) Family:
Family here refers to the plant family Rutaceae to which Glycosmis pentaphylla belongs. Understanding the family classification aids in identifying common characteristics and potential medicinal properties shared among related species, providing a broader context for the study of phytochemicals within herbal medicine.

31) Pitta (Pītta, Piṭṭā):
Pitta is one of the three doshas in Ayurveda representing fire and metabolism. Glycosmis pentaphylla is said to balance vitiated pitta, showing its potential role in Ayurvedic treatments. Studying compounds affecting dosha balance expands the understanding of traditional plants in holistic health practices.

32) Kapha:
Kapha is another of the three doshas in Ayurveda, embodying earth and water qualities. The use of Glycosmis pentaphylla for vitiated kapha indicates its application in Ayurvedic medicine to restore balance. Understanding such uses emphasizes the plant's significance in traditional health paradigms and herbal treatments.

33) Lanka (Laṅkā, Laṅka, Lamka, Lànkà):
Lanka refers to Sri Lanka, which shares cultural and botanical ties with southern India. The presence of Glycosmis pentaphylla in both regions emphasizes shared traditional medicinal practices. This connection enhances the understanding of cross-cultural herbal usage and the significance of regional flora.

34) Pandu (Pāṇḍu, Paṇḍu, Pandū, Pamdu, Pamde):
Pandu, meaning pale or light, traditionally describes conditions and represents a type of anemia in Ayurveda. Glycosmis pentaphylla’s use in treating pandu or anemia reinforces its role as a medicinal plant. The study’s findings on bioactive compounds may further validate its significance in managing such conditions.

35) Hindi (Hiṇḍī, Hiṃdī, Himdi, Hindī):
Hindi, the national language of India, signifies the cultural richness and diversity of the country. The mention of Hindi underscores the relevance of language in disseminating knowledge regarding traditional medicinal practices across regions, facilitating communication about the healing properties of plants like Glycosmis pentaphylla.

36) Cina (Cīna, Cīnā, Ciṉa, Ciṉā, Cīṉā, Cinā):
China is a neighboring country known for its rich herbal medicine practices similar to those in India. The mention of China highlights the widespread recognition of plants like Glycosmis pentaphylla across cultures, reflecting shared botanical knowledge and the potential for cross-cultural herbal exploration.

37) Vata (Vāṭa, Vaṭa, Vāta, Vaṭā):
Vata is one of the three doshas in Ayurveda representing movement and air. Glycosmis pentaphylla’s application in treating vitiated vata illustrates its importance in balancing bodily functions. This connection between the plant and dosha theory emphasizes its integral role in Ayurvedic medicinal practices.

38) Kula (Kūla, Kūḻā):
Kula in this context refers to the traditional names assigned to Glycosmis pentaphylla. It signifies the local knowledge surrounding the plant's identity, emphasizing the ethnobotanical significance and nurturing an understanding of how communities interact with local flora for medicinal purposes.

39) Ghat (Ghaṭ, Gham):
Ghat refers to the Western Ghats, a mountainous region rich in biodiversity. Glycosmis pentaphylla's growth in areas like Tirumala highlights the ecological significance of such regions. The study emphasizes the natural habitats where medicinal plants thrive, ensuring the preservation of these resources and their medicinal potential.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘GC-MS identification of bioactive compounds in Glycosmis pentaphylla.’. Further sources in the context of Science might help you critically compare this page with similair documents:

Phytochemical, Skin disease, Medicinal properties, Further studies, Rheumatism, Jaundice, Treatment of fever, Traditional medicine, Anaemia, Helminthiasis, Plant material, Herbarium specimen, Biological Activity, Folklore medicine, Toxic profile, Pharmacognosy, Ethanolic extract, Bioactive compound, Bioassay, Extraction method, Chemical constituent, GC-MS analysis, Mass spectrum, Biologically active constituents, Biochemical study, Traditional Practitioner, Major constituent, Various solvents, Hepatopathy, Bioactive constituent.

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