Callus production of globe artichoke and milk thistle
in vitro hypolipidemic and antioxidant activities
Journal name: World Journal of Pharmaceutical Research
Original article title: Callus production of globe artichoke and milk thistle
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Subtitle: in vitro hypolipidemic and antioxidant activities
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Shawky A. Bekheet, Mohamed K. El-Bahr, Sanaa A. Ali, Manal A. Hamed
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Callus production of globe artichoke and milk thistle
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
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Summary of article contents:
Introduction
This study, conducted by Shawky A. Bekheet and colleagues at the National Research Center in Cairo, Egypt, focuses on the in vitro callus production of two prominent medicinal plants: globe artichoke (Cynara scolymus L.) and milk thistle (Silybum marianum L.). The researchers aimed to optimize callus growth systems, evaluate the effects of specific growth regulators, and explore the hypolipidemic (lipid-lowering) and antioxidant activities of the extracts from callus cultures of both plants. By taking a biotechnological approach, this research seeks to address the challenges associated with traditional cultivation methods of these herbs, ensuring a sustainable supply of their active medicinal compounds.
Factors Affecting Callus Growth
In optimizing callus growth, the study presented significant findings on the influence of various gelling agents, with gelrite proving more effective than agar in solidifying the culture medium. Callus grown on gelrite exhibited higher growth rates, emphasizing the importance of nutrient diffusion as a key factor in the success of in vitro cultivation. The authors also examined the effect of picloram, a plant growth regulator, noting that a concentration of 3 mg/l resulted in optimal callus growth. Additionally, salicylic acid (SA) and jasmonic acid (JA) were investigated, with SA at 75 µM and JA at 50 µM showing enhanced callus development, particularly for milk thistle, which had a more pronounced response to these growth regulators compared to globe artichoke.
Hypolipidemic Activity of Callus Extracts
The study also delved into the hypolipidemic activities of the callus extracts through the inhibition of HMG-CoA reductase, an essential enzyme in cholesterol biosynthesis. In vitro experiments revealed that globe artichoke extracts exhibited a significantly higher inhibition rate of 85.77% at 100 mg, compared to milk thistle's 69.86%. This indicated that globe artichoke is potentially more effective than milk thistle in reducing lipid levels, corroborating previous clinical reports and emphasizing the therapeutic potential of these plants in managing hyperlipidemia.
Antioxidant Properties of Extracts
In addition to their lipid-lowering effects, the callus extracts displayed notable antioxidant properties, assessed through DPPH free radical scavenging activity. The findings showed that both globe artichoke and milk thistle extracts outperformed their standard compounds (cynarin and silymarin) at various concentrations, showcasing their potential as natural antioxidants. Moreover, the antioxidant activities were linked to the total flavonoid content of the extracts, supporting the idea that the medicinal benefits of these plants extend beyond just lipid reduction, offering protection against oxidative stress.
Conclusion
The research underscores the potential of plant tissue culture technology in producing the active medicinal components of globe artichoke and milk thistle, providing a reliable source of these beneficial compounds year-round. The study demonstrates that optimizing cultural conditions and using specific growth regulators can significantly enhance callus growth and the extraction of bioactive compounds. Additionally, the results suggest that both plants could serve as valuable natural alternatives for managing lipid profiles and oxidative damage. By combining biotechnological advances with traditional herbal remedies, the findings contribute to a deeper understanding of these medicinal plants and their applications in modern pharmacotherapy.
FAQ section (important questions/answers):
What were the main goals of the study on globe artichoke?
The study aimed to develop an in vitro callus growth system for globe artichoke and milk thistle, while also investigating their hypolipidemic and antioxidant activities.
Which gelling agent showed superior callus growth in the experiment?
Gelrite exhibited higher growth of callus compared to agar, leading to better fresh weight and growth values for both globe artichoke and milk thistle.
How did picloram affect callus growth in the study?
Picloram significantly enhanced callus growth, with the best results observed at 3 mg/l for both globe artichoke and milk thistle.
What was the effect of salicylic acid on callus growth?
Salicylic acid positively influenced callus growth, particularly at 75 µM, improving fresh weight and dry matter of calli from both plant species.
What were the findings related to hypolipidemic activity?
The callus extracts showed hypolipidemic effects in a dose-dependent manner, with globe artichoke extract exhibiting more potent inhibition of HMG-CoA reductase than milk thistle.
What does the antioxidant activity of the extracts indicate?
The extracts of globe artichoke and milk thistle displayed significant antioxidant effects, surpassing their respective standards, thus highlighting their potential health benefits.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Callus production of globe artichoke and milk thistle”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Milk:
Milk thistle (Silybum marianum) is a vital species in this study, known for its active compounds, particularly silymarin, which has hepatoprotective properties. Its relevance lies in its potential therapeutic effects on liver diseases and its utility in producing antioxidants, making it a subject of in vitro investigations.
2) Medium:
The term 'medium' refers to the culture medium used for callus induction in plant tissue cultures. It provides essential nutrients and growth hormones, such as MS medium (Murashige and Skoog). The composition of the medium directly influences callus growth and the extraction of pharmacologically active compounds.
3) Activity:
Activity pertains to the hypolipidemic and antioxidant properties observed in the extracts of globe artichoke and milk thistle callus cultures. Evaluating these biological activities helps to determine the effectiveness of these extracts in reducing lipid levels and combating oxidative stress, which are significant for therapeutic applications.
4) Table:
Tables in the study summarize results and facilitate understanding of experimental data. They present findings such as callus growth metrics, hypolipidemic effects, and antioxidant activities, allowing for clear comparisons between different treatments and aiding researchers in analyzing trends and differences in plant responses.
5) Species:
This term refers to the specific plants studied, notably globe artichoke and milk thistle. Both species are relevant for their medicinal properties, especially concerning liver health and their ability to produce bioactive compounds through tissue culture systems, which are crucial for pharmaceutical applications.
6) Water:
Water is a critical component of the experimental protocols. It is used for various purposes, including seed sterilization, dissolving growth regulators, and as a part of the culture medium. Its quality affects nutrient availability and, subsequently, the success of callus induction and growth.
7) Study (Studying):
The study aims to optimize callus production of globe artichoke and milk thistle and evaluate their hypolipidemic and antioxidant activities. Through biotechnological approaches, the research seeks to offer alternatives to traditional agricultural methods for producing active compounds beneficial in pharmaceuticals.
8) Egypt:
Egypt, where the research is conducted, has a rich tradition of herbal medicine, and the presence of globe artichoke and milk thistle is significant for local medicinal practices. The country's agricultural landscape influences the availability and study of these valuable plant species for pharmaceutical use.
9) Drug:
The study explores the potential of extracts from the callus cultures of globe artichoke and milk thistle as natural drugs. These plants have generated interest for their pharmacological benefits, particularly in treating liver diseases and hyperlipidemia, addressing the need for effective, plant-derived therapeutic agents.
10) Accumulation (Accumulating, Accumulate):
Accumulating refers to the process by which the callus cultures of globe artichoke and milk thistle gather beneficial compounds over time. This aspect is critical in assessing the efficiency of tissue cultures in producing higher concentrations of pharmaceutical-grade natural products for use in medicine.
11) Flavonoid:
Flavonoids are a class of bioactive compounds present in globe artichoke and milk thistle. Their antioxidant properties are crucial for protecting cells from oxidative stress. Understanding their accumulation in the studied plants helps evaluate their potential health benefits and applications in natural medicine.
12) Disease:
The study is relevant for addressing diseases related to liver dysfunction and hyperlipidemia. By investigating the medicinal properties of globe artichoke and milk thistle, the research contributes to the pool of natural treatments available for chronic diseases, emphasizing plant-based solutions for health challenges.
13) Animal:
Animal studies are often referenced in the research to support the claims regarding the efficacy of globe artichoke and milk thistle extracts. These studies help to establish the safety and pharmacological effects of plant compounds, bridging the gap between in vitro findings and potential clinical applications.
14) Shana (Śaṇa, Śana, Sāṇa, Saṇa, Sanā, Sana, Śānā, Saṅa):
Sanaa Ahmed Ali is one of the authors of the study and the corresponding author. Her role includes overseeing the project, ensuring the integrity of the research, and facilitating communication concerning the study's findings, contributing to the advancement of knowledge in plant biotechnology.
15) Hand:
In the context of this study, 'hand' may refer to the methodical care and manual techniques involved in plant tissue culture processes. Proper handling of plant materials, such as explants, is crucial for successful callus induction and growth, directly impacting the research outcomes.
16) Observation:
Observation is key in the experimental design, emphasizing the importance of monitoring the growth and responses of the callus cultures to various growth regulators. Documenting these observations helps analyze the effectiveness of treatments and derive conclusions about optimal conditions for cultivation.
17) Measurement:
Measurement in the study involves quantifying various parameters, such as fresh weight, dry matter, and percentage inhibition of enzymes. Accurate measurements are essential for evaluating the growth of callus and the pharmacological effects of the extracts, allowing for meaningful comparisons and data analysis.
18) Agriculture:
Agriculture forms the foundation of this study by exploring the potential of biotechnological approaches to enhance the production of valuable medicinal plants. By addressing agricultural limitations, such as seasonal growth and disease susceptibility, innovative tissue culture methods can fulfill pharmaceutical demands.
19) Discussion:
Discussion is a vital section in the study, where results are interpreted, compared with existing literature, and implications are drawn. It provides insights into the significance of findings, potential applications, and future research directions, facilitating a deeper understanding of the study's contributions to the field.
20) Substance:
Substance refers to the chemical compounds of interest in the study, particularly phytochemicals derived from the callus cultures of globe artichoke and milk thistle. Understanding the nature and effects of these substances is crucial for evaluating their therapeutic potential and applications in treating various health conditions.
21) Seedling:
Seedling propagation is significant for establishing the initial plant material used in callus induction. The health and genetic quality of seedlings ensure successful culturing processes, affecting the yield and quality of the bioactive compounds produced for further pharmaceutical research.
22) Quality:
Quality relates to the efficacy and bioactive potential of the extracts derived from callus cultures. Assessing the quality of these extracts is essential for determining their suitability for medicinal applications, indicating the need for optimization in culture conditions to enhance the therapeutic properties.
23) Nature:
Nature pertains to the origin and ecological significance of globe artichoke and milk thistle. Both plants are valued not only for their medicinal properties but also for their role in biodiversity. Understanding their natural context aids in conserving these species while promoting sustainable practices in their cultivation.
24) Powder:
Powder refers to the processed form of dried extracts from globe artichoke and milk thistle. This powdered form is often used in pharmacological studies and supplements, allowing for standardized dosing and better incorporation into various therapeutic formulations aimed at liver health and lipid management.
25) Bile:
Bile acids are involved in digestion and fat absorption, and the study of globe artichoke supports its traditional use for enhancing bile production and secretion. This property is crucial for its hypolipidemic effects, as improved bile flow can aid in lowering cholesterol levels.
26) Rice (Ṛce):
Rice is mentioned as a comparative example for assessing tissue culture techniques. The study draws comparisons with established tissue culture methods used for rice to underline the effectiveness of biotechnological approaches in promoting the growth and yield of other plant species.
27) Rich (Ṛch):
Rich describes the abundance of bioactive compounds found in globe artichoke and milk thistle. This richness underscores the potential of these plants for pharmaceutical applications, highlighting their capacity to provide a diverse range of therapeutic agents that can address various health issues.
28) Wall:
Wall may refer to the structural aspects of plant tissues, such as cell walls which are important for callus formation and growth. Understanding cell wall dynamics is crucial in manipulating culture conditions to enhance callus induction and subsequent accumulation of active compounds.
29) Viru (Vīṟu):
Virulence is central to understanding the challenges faced in traditional agricultural practices. The study explores alternatives to traditional methods that often succumb to viral diseases affecting crops. By optimizing tissue cultures, the potential to produce disease-free and consistent plant material is emphasized.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Callus production of globe artichoke and milk thistle’. Further sources in the context of Science might help you critically compare this page with similair documents:
Active agent, Natural product, Statistical analysis, Culture, Antioxidant activity, Chronic liver disease, In vitro, Hepatoprotective properties, Radical Scavenging Activity, Antioxidant activities, Hypolipidemic activities, Hypocholesterolemic activities, Lipid metabolism, In vitro antioxidant activity, DPPH free radical, One-way analysis of variance, Silymarin, Hypolipidaemic activity, Salicylic acid, Hepatoprotective agent, Absorbance measurement, Growth Medium, Callus induction, Antioxidant enzyme activities, Enzymatic activity, Culture medium, Gelling agent, Cholesterol absorption, Fresh weight, Commercial production, Enzyme Activity, HMG-CoA reductase, Callus growth, Phenolic compound, Callus culture, In vitro culture, Chemical component, Plant growth regulator, HMG-CoA reductase enzyme.