Evaluation of antibacterial activity of some medicinal plants
Journal name: World Journal of Pharmaceutical Research
Original article title: Evaluation of antibacterial activity of some medicinal plants
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Original source:
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Ansar M. Patel, Dr. Rudraprabhu. V. Savadi
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Evaluation of antibacterial activity of some medicinal plants
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
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Summary of article contents:
Introduction
Infectious diseases are a leading cause of mortality, exacerbated by factors such as pollution, overpopulation, and changing environmental conditions. Increased use of antimicrobial drugs has led to resistance among microorganisms and heightened toxicity in humans and animals. This study aimed to evaluate the antibacterial properties of herbal extracts from Erythrina indica leaves, Bergenia ciliata rhizomes, and Cissampelos pareira stems against five pathogenic bacterial strains: Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, Escherichia coli, and Salmonella typhi.
Efficacy of Ethanol vs. Aqueous Extracts
The research demonstrated that ethanolic extracts of the selected plants exhibited significantly higher antibacterial activity compared to their aqueous counterparts. The agar cup plate method revealed that the ethanolic extracts at various concentrations effectively inhibited the growth of targeted bacteria, with the 5 mg/ml concentration showing particularly remarkable results. This indicates that the extraction solvent plays a critical role in the potency of the antibacterial activity, suggesting a preferential use of ethanol over water for these specific extracts.
Antibacterial Spectrum Against Specific Bacteria
Each plant extract showed a unique antibacterial profile. For instance, the ethanolic extract of Bergenia ciliata displayed the highest activity against Escherichia coli and Bacillus cereus, while Erythrina indica and Cissampelos pareira extracts exhibited potent effects against Staphylococcus aureus, Salmonella typhi, and Bacillus subtilis. The results highlight the effectiveness of these herbal remedies against both Gram-positive and Gram-negative bacteria, suggesting their potential utility in treating bacterial infections.
Potential for Herbal Medicine Development
The findings underscore the therapeutic potential of using herbal extracts as antimicrobial agents. Given the rising issues surrounding antibiotic resistance, these natural alternatives could provide a safe and effective means of infection management. The study prompts further exploration into the specific compounds within these plants responsible for their antibacterial properties, which could lead to the development of new antimicrobial drugs.
In conclusion, the study successfully demonstrated that ethanolic extracts of Erythrina indica, Bergenia ciliata, and Cissampelos pareira possess significant antibacterial activity against a range of pathogenic bacteria. The extracts' effectiveness surpasses that of aqueous extracts, supporting their potential as a source for new antimicrobial agents. This research provides a basis for the continued investigation into plant-derived antimicrobials, which could be integral in combatting bacterial resistance and developing sustainable treatment options.
FAQ section (important questions/answers):
What was the objective of the study regarding medicinal plants?
The study aimed to determine the antibacterial activity of ethanolic and aqueous extracts from Erythrina indica leaves, Bergenia ciliata rhizome, and Cissampelos pareira stem against five pathogenic bacterial strains.
Which bacterial strains were tested for antibacterial activity?
The bacterial strains tested included Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, Escherichia coli, and Salmonella typhi.
How were the antibacterial activities evaluated in the study?
Antibacterial activities were evaluated using the agar cup plate method on sterile nutrient agar, comparing zones of inhibition of plant extracts with those of standard Ampicillin.
Which extracts showed greater antibacterial activity, ethanolic or aqueous?
Ethanolic extracts of Erythrina indica, Bergenia ciliata, and Cissampelos pareira showed greater antibacterial activity compared to their aqueous extracts.
What was the conclusion regarding the studied medicinal plants?
The study concluded that ethanolic extracts of the plants showed promising antibacterial activity against both gram-positive and gram-negative bacteria, indicating potential for developing new antimicrobial agents.
What measurements were taken during the study to assess antibacterial activity?
The zone of inhibition around each extract was measured in millimeters after incubation, providing a quantifiable assessment of antibacterial activity.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Evaluation of antibacterial activity of some medicinal plants”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Antibiotic (Antibacterial):
Antibiotic refers to substances that can kill or inhibit bacterial growth, commonly applied in modern medicine. The study contrasts the effectiveness of traditional herbal plant extracts against conventional antibiotics like Ampicillin, highlighting an increasing need to explore alternative antimicrobial therapies.
2) Activity:
Activity in this study denotes the effectiveness of the plant extracts against bacterial strains. It is a crucial measurement of their antibacterial potency, typically represented by the 'zone of inhibition'. Understanding the activity is vital for assessing the therapeutic potential of herbal medicines in combating infections.
3) Aureus:
Aureus specifically refers to Staphylococcus aureus, a pathogenic bacterium responsible for various infections ranging from minor skin issues to serious systemic conditions. The study investigates how the extracts interact with this bacterium, thereby contributing to the development of effective antibacterial agents targeting such pathogens.
4) Table:
Table refers to the structured data presentation used in the study to summarize the experimental results. It facilitates the clear comparison of the antibacterial activity between different plant extracts and the standard drug Ampicillin, allowing for easy analysis of the findings and discussion of the efficacy of the extracts.
5) Medium:
Medium refers to the nutrient agar used in the study, which supports the growth of the targeted bacterial strains. The choice of medium is fundamental in microbiological research, as it ensures the bacteria can grow adequately for testing the antibacterial properties of the plant extracts under controlled conditions.
6) Drug:
Drug in this context refers to the substances intended to treat bacterial infections, such as Ampicillin. The study explores alternative drugs derived from medicinal plants, emphasizing the increasing need for new antimicrobial agents in light of rising drug resistance in pathogenic bacteria.
7) Maharashtra (Mahārāṣṭra, Maha-rashtra):
Maharashtra is a state in India where the research was conducted, providing geographical context to the study. The state is known for its biodiversity, and the native medicinal plants investigated may have unique properties that contribute to the findings of the antibacterial evaluations.
8) Medicine:
Medicine refers to the field focused on healing and treatment of diseases. In this study, the exploration of herbal medicine emphasizes the potential of naturally derived substances to offer alternative solutions to conventional antibacterial therapies, particularly in areas with rising antimicrobial resistance.
9) India:
India, the country where the research is based, has a rich history of traditional medicine, including the use of herbal remedies. The study highlights the importance of investigating and validating locally used medicinal plants as potential sources of new antimicrobial agents.
10) Water:
Water is significant in the study for its role as a solvent in preparing aqueous extracts of the plant materials. The comparison between the efficacy of ethanolic and aqueous extracts helps ascertain the optimal extraction techniques for enhancing the antibacterial properties of the plants.
11) Rajasthan (Rājasthān):
Rajasthan is a state in India known for its biodiversity and traditional medicinal practices. Mentioning Rajasthan provides context regarding the potential local availability of the herbal materials used in the study, shedding light on the ethnobotanical relevance of the researched plants.
12) Kolhapur (Kolhāpur):
Kolhapur is a city in Maharashtra, India, where the plant materials were collected and identified. The significance lies in the locality's unique flora, which may contribute to discovering novel antibacterial properties of the plants studied, offering insights into regional medicinal practices.
13) Patel (Paṭel, Pāṭel):
Patel is the surname of the primary author, Ansar M. Patel, who conducted this research study. Recognizing the author's contribution is essential for academic attribution and highlights the importance of individual researchers in advancing knowledge in the field of pharmacology and medicinal plant research.
14) Study (Studying):
Study refers to the systematic investigation conducted to assess the antibacterial properties of certain plant extracts. The rigour of the study provides empirical evidence supporting the potential utilization of these plants in traditional and modern medicine as antimicrobial agents.
15) Shri (Śri, Śrī, Śṝ, Sṛ, Sṝ):
Shri is an honorific prefix used before names in India, indicating respect. Here, it precedes 'Jagdishprasad Jhabarmal Tibrewala University', underscoring the academic institution's role in the research and validating the scholarly framework supporting the investigation of medicinal plants.
16) Discussion:
Discussion pertains to the section of the study that interprets the results, comparing findings with existing literature. This critical analysis is vital for understanding the implications of the outcomes, considering the practicality of using herbal medicine in modern therapeutic approaches against bacterial infections.
17) Science (Scientific):
Scientific refers to the rigorous methodology employed in the study to ensure valid and reliable results. The emphasis on scientific evaluation helps in substantiating claims regarding the antibacterial activities of the plant extracts, contributing to the overall body of knowledge in pharmacology.
18) Observing:
Observing refers to the process of watching or monitoring the effects of the medicinal plant extracts on bacterial strains during the study. This critical observational aspect enables researchers to determine the antibacterial efficacy and potential therapeutic applications of the extracts.
19) Toxicity:
Toxicity is an important consideration in evaluating new medicinal compounds. In this study, the lower toxicity profiles of herbal extracts compared to conventional drugs highlight their safety as therapeutic agents, reinforcing their potential use in treating infections without significant adverse effects on patients.
20) Disease:
Disease refers to any abnormal condition caused by pathogens, including bacterial infections. The study aims to address the rising incidence of such diseases, emphasizing the necessity for discovering new and effective treatments derived from natural sources to combat these health challenges.
21) Species:
Species denotes the various pathogenic bacteria tested in the study, highlighting the focused approach of assessing multiple strains to determine the antibacterial efficacy of the plant extracts. This broad spectrum analysis is crucial for understanding the potential applicability of the extracts in diverse infections.
22) Shatara (Satara, Śatāra, Śaṭara):
Satara is a district in Maharashtra, India, where one of the authors is affiliated. Its mention connects the research to the regional academic environment and facilities available for conducting such scientific studies on antimicrobial properties of herbal medicine.
23) Shavadi (Śāvaḍi):
Savadi is the surname of Dr. Rudraprabhu V. Savadi, co-author of the study. Acknowledging his contribution reflects the collaborative effort essential in scientific research, showcasing the importance of mentorship and guidance in academic pursuits within the field.
24) Animal:
Animal pertains to the consideration of toxicity and safety profiles of the tested plant extracts. Understanding the potential impact of these extracts on animal models is crucial for advancing to human trials, ensuring that the herbal treatments do not pose risks to health in practical applications.
25) Filling (Filled):
Filled refers to the process of introducing the plant extracts into the agar wells for evaluating their antibacterial activity. This methodological step is significant as it ensures proper delivery and uniform distribution of extracts, facilitating accurate measurement of their inhibitory effects on bacterial growth.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Evaluation of antibacterial activity of some medicinal plants’. Further sources in the context of Science might help you critically compare this page with similair documents:
Cissampelos pareira, Herbal medicine, Cultural practice, Antibacterial activity, Zone of inhibition, Aqueous extract, Ethanolic extract, Nutrient agar, Gram positive bacteria, Gram negative bacteria, Phytomedicine, Microbial infection.