Effect of Cynodon dactylon on free radicals in Clarias batrachus.

| Posted in: Science

Journal name: World Journal of Pharmaceutical Research
Original article title: Effect of cynodon dactylon on free radical load of lymphoid organs of asian catfish, clarias batrachus
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Original source:

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Author:

Mishra, P. Jurry, R., and Gupta, S.


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Effect of cynodon dactylon on free radical load of lymphoid organs of asian catfish, clarias batrachus

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research


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Summary of article contents:

Introduction

The study investigates the effects of aqueous and alcoholic extracts of Cynodon dactylon on the oxidative stress levels in the lymphoid organs (spleen, thymus, and head kidney) of Asian catfish (Clarias batrachus). Free radicals, known for their instability and potential to cause cellular damage, can lead to various health issues. Antioxidant defense mechanisms in organisms can protect against such oxidative stress, and the research aims to evaluate whether Cynodon dactylon, a common medicinal plant, can enhance these defenses in fish under stress from environmental factors.

Effect on Antioxidant Enzymes

The application of both extracts resulted in a significant increase in the activities of superoxide dismutase (SOD) and catalase (CAT) in the lymphoid organs of C. batrachus. These enzymes play crucial roles in neutralizing free radicals and minimizing oxidative stress. The results indicated a more pronounced effect at higher doses, suggesting that the extracts of Cynodon dactylon enhance the antioxidant defenses of the fish, thereby potentially improving their health and resilience against environmental stressors.

Impact on Lipid Peroxidation

The study also reported a notable reduction in lipid peroxidation levels in the lymphoid organs following treatment with the plant extracts. Lipid peroxidation is a marker of oxidative damage and its reduction is indicative of improved cellular integrity and function. The results support the notion that Cynodon dactylon possesses protective qualities against oxidative damage by decreasing the byproducts associated with lipid degradation, which is critical for maintaining healthy cellular membranes.

Pharmacological Implications

Cyodon dactylon is rich in various bioactive compounds, including flavonoids, tannins, and terpenoids, which are believed to contribute to its antioxidant properties. These compounds function by scavenging free radicals and inhibiting various stages of oxidative reactions, thereby contributing to the plant's therapeutic potential. The confirmed presence of these compounds in the extracts used in this study suggests that they may be responsible for the observed antioxidant effects, further supporting the plant's utility in managing oxidative stress in aquatic organisms.

Conclusion

The research demonstrates that extracts of Cynodon dactylon effectively enhance the antioxidant activity in Clarias batrachus, marked by increased SOD and CAT activity and decreased lipid peroxidation. These findings indicate the potential of the plant as a natural antioxidant source, promoting fish health. Further investigations are recommended to isolate and identify the specific active compounds responsible for these effects and to better understand the mechanisms underlying the antioxidant activity in fish.

FAQ section (important questions/answers):

What was the aim of the study on Clarias batrachus?

The study aimed to assess the effect of aqueous and alcoholic extracts of Cynodon dactylon on the free radical load in the lymphoid organs (spleen, thymus, and head kidney) of Asian catfish, Clarias batrachus.

How were the fish treated with Cynodon dactylon extracts?

Thirty-six mixed-sex fish were divided into three groups: one control group and two test groups treated with 50 ppm and 100 ppm of Cynodon dactylon extracts, administered every alternate day for 28 days.

What were the results regarding antioxidant enzymes and lipid peroxidation?

Cynodon dactylon treatment significantly increased superoxide dismutase (SOD) and catalase (CAT) levels while decreasing lipid peroxidation in the lymphoid organs compared to the control group.

What methodology was used for the enzyme assays in the study?

The lymphoid organs were homogenized and analyzed using established methods for measuring SOD, CAT, and lipid peroxidation levels, ensuring systematic data collection for statistical analysis.

What are the potential health benefits of Cynodon dactylon for fish?

The antioxidant properties of Cynodon dactylon may improve fish health by reducing oxidative stress indicated by increased antioxidant enzyme activity and lowered lipid peroxidation, potentially enhancing immune function.

What future research directions are suggested in the study's conclusion?

The study recommends further research to isolate active compounds in Cynodon dactylon and to elucidate the mechanisms underlying its antioxidant properties in fish.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Effect of Cynodon dactylon on free radicals in Clarias batrachus.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Activity:
The term 'activity' in this context refers to the biological effects measured during the experimentation, specifically the enhancement of antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT). These activities highlight the efficacy of extracts in reducing oxidative stress and protecting fish health from free radical damage.

2) Cynodon dactylon:
Cynodon dactylon, also known as doob grass, is a medicinal plant used in this study. Its extracts were evaluated for antioxidant properties against oxidative stress in the Asian catfish, Clarias batrachus. The plant is noted for its potential therapeutic benefits, particularly in enhancing immune functions.

3) Mishra (Miśra):
Mishra refers to the lead author of the study whose research contributes to understanding the antioxidant effects of Cynodon dactylon on Clarias batrachus. The findings advance knowledge in the field of aquaculture and pharmacology, showcasing the importance of natural extracts in improving fish health.

4) Fish:
Fish, specifically Clarias batrachus, is the subject of this study, which aims to assess the antioxidant properties of plant extracts. Fishes are vital in ecosystems and human diets, making research on their health and the impact of natural remedies crucial for sustainable aquaculture practices.

5) India:
India is the geographical context of this research, where Cynodon dactylon is prevalent. The study reflects the country's rich biodiversity and traditional use of medicinal plants. It emphasizes the importance of local resources in improving fish health and contributes to the broader field of ethnopharmacology.

6) Flavonoid:
Flavonoids are bioactive compounds present in Cynodon dactylon that exhibit antioxidant properties. They play a crucial role in the plant's ability to scavenge free radicals, thereby protecting cells from oxidative damage. Their influence in enhancing antioxidant enzyme activities makes them significant in aquaculture.

7) Study (Studying):
The term 'study' encapsulates the systematic investigation into the effects of Cynodon dactylon extract on antioxidant levels in fish. It aims to provide scientific evidence for the therapeutic roles of medicinal plants in enhancing fish health and combating oxidative stress, thus contributing to aquaculture research.

8) Water:
Water is the essential medium in which Clarias batrachus resides. The study's experimental protocol emphasizes the importance of water quality and conditions in aquaculture, as it directly influences fish health and the effectiveness of treatment with Cynodon dactylon extracts.

9) Species:
In this context, species refers specifically to Clarias batrachus, the Asian catfish under investigation. Understanding how various species respond to treatments such as Cynodon dactylon extracts is essential for developing species-specific practices in aquaculture and enhancing their health through natural remedies.

10) Science (Scientific):
Science refers to the systematic study and application of knowledge, particularly in understanding biological processes. This research contributes to the scientific understanding of antioxidant mechanisms in fish, showcasing the intersection of traditional remedies and modern scientific approaches in improving aquaculture practices.

11) Table:
Table in this context presents important quantitative data regarding the effects of Cynodon dactylon extracts on SOD and CAT activity in fish. It serves as a visual summary that aids in interpreting and analyzing results, making the findings accessible for further research and application.

12) Ocimum sanctum:
Ocimum sanctum, or holy basil, is another medicinal plant mentioned in the study. Its inclusion alongside Cynodon dactylon highlights the diversity of traditional herbs used for therapeutic purposes in India. Both plants are noted for their health-promoting properties and potential in enhancing fish health.

13) Pharmacological:
Pharmacological pertains to the study of drug actions, including the effects of plant extracts on living organisms. This research investigates the pharmacological relevance of Cynodon dactylon, emphasizing its antioxidant properties and potential therapeutic applications in managing oxidative stress in fish.

14) Container:
Container refers to the ice-cold vessels used for storing collected lymphoid organs in the study. The use of appropriate containers ensures the preservation of samples for enzyme assays, thereby maintaining the integrity of the biological materials before analysis.

15) Disease:
Disease in this context relates to health issues in fish, often exacerbated by oxidative stress and free radical damage. The study investigates how Cynodon dactylon extract might mitigate such conditions, highlighting the plant's potential role in preventing diseases linked to oxidative damage.

16) Zoology:
Zoology is the scientific study of animals, encompassing aspects of their biology, physiology, and ecology. The research connects to zoology by investigating the health of Clarias batrachus, contributing to the broader understanding of animal biology in relation to environmental stressors and treatments.

17) Family:
Family refers to the taxonomic classification of Cynodon dactylon, which belongs to the Poaceae (grass family). Understanding its classification helps in the study of its ecological role and potential uses in medicinal applications, especially in the context of traditional remedies in agriculture.

18) Blood:
Blood is vital for transporting oxygen and nutrients within fish, playing a crucial role in their overall health. The research examines how Cynodon dactylon impacts immune function within lymphoid organs, indirectly influencing blood health by enhancing antioxidant defenses against free radicals.

19) Tinospora cordifolia:
Tinospora cordifolia is another medicinal plant cited in the study, known for its health benefits. Its mention supports the idea that various herbs can provide antioxidant support in different biological models, reinforcing the premise that plant-derived compounds are significant for health improvement.

20) Burning sensation:
Burning sensation refers to a potentially painful experience, often resulting from inflammation or irritation. References to traditional uses of medicinal plants, such as Cynodon dactylon, for alleviating burning sensations illustrate the role of herbal remedies in managing discomfort and health issues.

21) New Delhi:
New Delhi, the capital of India, represents the socio-political context in which this research might have implications. Studies like this contribute to public health discussions on utilizing indigenous plants for health benefits, emphasizing the importance of local resources in managing fish health.

22) Allium sativum:
Allium sativum, commonly known as garlic, is referenced as having similar antioxidant effects. Its inclusion highlights the importance of various plants in traditional medicine. The comparison with Cynodon dactylon emphasizes the wider array of natural remedies available for enhancing health in biological systems.

23) Antibiotic (Antibacterial):
Antibacterial refers to the property of substances that inhibit bacterial growth. The mention of antibacterial properties in the study points toward the broader benefits of Cynodon dactylon, not only in combating oxidative stress but also in providing defense against pathogens affecting fish health.

24) Malnutrition:
Malnutrition relates to deficiencies in essential nutrients, impacting fish growth and health. Citing malnutrition in the study underscores the necessity of antioxidants like those from Cynodon dactylon, which may help mitigate effects of a poor diet by supporting overall health and immune function.

25) Discussion:
Discussion is a critical component of the research, where results are interpreted in the context of existing literature. This section synthesizes findings, offering insights into the antioxidant activity of Cynodon dactylon and its implications for fish health and broader pharmacological applications.

26) Leucoderma:
Leucoderma refers to skin conditions characterized by loss of pigmentation. The traditional use of Cynodon dactylon for skin health signals its broader medicinal properties, indicating potential applications beyond fish studies into human health, reflecting the plant's historical significance in treatments.

27) Snake-bite:
Snake-bite refers to a serious medical condition often requiring immediate treatment. Mentioning it in the context of Cynodon dactylon highlights traditional practices where plants are utilized for their purported healing properties, establishing the plant's significance in folk medicine and its potential pharmacological benefits.

28) Substance:
Substance refers broadly to the chemical components found in Cynodon dactylon that are active in antioxidant activity. Understanding these substances is vital for isolating active compounds that could lead to the development of natural remedies aimed at enhancing health and combating oxidative stress in fish.

29) Toxicity:
Toxicity in this context concerns the harmful effects of oxidative stress and free radicals on living organisms. The study of Cynodon dactylon's effects emphasizes its potential to reduce toxicity levels in fish, highlighting the plant's protective role against harmful environmental factors.

30) Swelling:
Swelling is an inflammatory response that can occur due to various health issues. The traditional use of Cynodon dactylon for treating swelling illustrates its medicinal potential, providing insights into how plant extracts may help alleviate inflammation in fish as well as in other animals.

31) Mineral:
Mineral refers to essential nutrients that play vital roles in physiological processes. The presence of minerals in Cynodon dactylon underscores its comprehensive health benefits, supporting immune functions in fish, thereby contributing to their overall well-being and resilience against diseases.

32) Burning (Burn, Burned, Burnt):
Burning in a health context often describes a painful or uncomfortable sensation. The study references traditional uses of Cynodon dactylon to relieve such symptoms, suggesting that its antioxidant properties may translate into benefits for managing inflammatory responses and associated discomforts.

33) Ganesha (Gaṇeśa, Gāṇeśa, Ganeśa, Gana-isha):
Ganesha is a widely revered deity in Hinduism, often associated with wisdom and auspicious beginnings. The cultural significance of Cynodon dactylon, as it is used in religious practices, illustrates the plant's deep-rooted importance in Indian traditions, complementing its medicinal applications.

34) Animal:
Animal refers generically to living organisms within the kingdom Animalia, which includes fish examined in the study. Research on antioxidant benefits for animals like Clarias batrachus provides valuable insights that may extend to broader animal health and conservation efforts.

35) Powder:
Powder refers to the processed form of Cynodon dactylon used to prepare extracts in the study. This powdered form facilitates the extraction of bioactive compounds, enabling researchers to evaluate the plant's beneficial effects on fish health through scientifically standardized treatments.

36) Thirst:
Thirst relates to the body's need for hydration, and plants like Cynodon dactylon, traditionally used for alleviating thirst, demonstrate their medicinal properties beyond oxidative stress relief. Such uses highlight the multifaceted benefits of indigenous plants in addressing various health concerns.

37) Botany:
Botany is the study of plant life, encompassing the classification, physiology, and ecology of plants like Cynodon dactylon. The research highlights botany's significance in understanding plant properties and ecological roles, contributing to the development of natural remedies for health improvement.

38) Gupta (Guptā):
Gupta is one of the co-authors of the study, contributing to the research on Cynodon dactylon's effects. The involvement of multiple researchers emphasizes collaboration in scientific inquiry, which enhances the robustness and credibility of the findings presented in aquatic health sciences.

39) Delhi:
Delhi refers to the capital city of India, where significant research and academic activities occur. The mention of Delhi situates the study within a broader socio-scientific context, suggesting potential implications and applications of the findings in urban environmental management and aquaculture practices.

40) Simha (Siṃha):
Sinha is one of the researchers whose methods were utilized for enzyme assays in the study. The mention of Sinha underscores the importance of established techniques in research, enabling the accurate measurement of enzyme activities that support claims regarding the antioxidant effects of Cynodon dactylon.

41) Joshi (Jōśī, Jośī):
Joshi denotes another researcher referenced for disinfection protocols prior to the experiments. This illustrates the importance of meticulous scientific procedures in research, ensuring that results are credible and can be reproduced, which is essential for validating findings in biological studies.

42) Honey:
Honey is noted for its natural health benefits and has been associated with various medicinal properties. Including honey in discussions of medicinal properties reflects the broader context of how natural substances are valued in traditional healing practices, advocating for holistic approaches to health.

43) Fever:
Fever is commonly triggered by infections or inflammation and represents a significant health challenge. Mentioning its traditional treatments with plants like Cynodon dactylon highlights historical practices that reflect the application of herbal remedies in managing various physiological conditions.

44) Genu (Geṇu):
Genu refers to a joint in the body, indicating structural anatomical aspects. While not directly emphasized in the study, acknowledging anatomical terminology reflects the comprehensive nature of biological research and may highlight how plant remedies like Cynodon dactylon can be evaluated for joint-related health benefits.

45) Rich (Ṛch):
Rich describes the abundance of certain components in plants or ecosystems, like nutrients and bioactive compounds in Cynodon dactylon. The richness of these elements underscores their potential health benefits, promoting the importance of biodiversity in providing resources for medicinal applications in aquaculture.

46) Soil:
Soil refers to the natural medium for plant growth, critical for cultivating Cynodon dactylon. The health of soil plays a vital role in the production of medicinal plants, impacting their quality and the efficacy of bioactive compounds derived from them in health-promoting applications.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Effect of Cynodon dactylon on free radicals in Clarias batrachus.’. Further sources in the context of Science might help you critically compare this page with similair documents:

Cynodon dactylon, Therapeutic use, Phytochemical screening, Reactive oxygen species, Lipid peroxidation, Oxidative damage, ANOVA, Superoxide dismutase, Catalase, Free Radical.

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