Effects of plant extracts on Klebsiella pneumonia in UTI cases.
Journal name: World Journal of Pharmaceutical Research
Original article title: In vitro and in vivo effect of three aqueous plant extract on pathogenicity of klebsiella pneumonia isolated from patient with urinary tract infection
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Sura hsan Abed Chabuck, Nagham Adil Ghani Chabuck
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: In vitro and in vivo effect of three aqueous plant extract on pathogenicity of klebsiella pneumonia isolated from patient with urinary tract infection
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
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Summary of article contents:
Introduction
The study aimed to evaluate the antibacterial activity of four aqueous plant extracts (pomegranate, apple, lemon, and orange peels) against Klebsiella pneumoniae isolated from urinary tract infection patients. This bacterium is known for its role in both community and hospital-acquired infections, displaying significant resistance to antibiotics. Given the rising concern regarding antibiotic resistance, there is a pressing need for alternative antimicrobial agents from natural sources. The research highlights the potential of using plant extracts, traditionally employed in medicine, as a viable option against such pathogenic infections.
Antibacterial Efficacy of Pomegranate Extract
The findings revealed that pomegranate peel extract exhibited the highest antibacterial activity against K. pneumoniae. At concentrations from 1000 mg/mL and higher, the extract significantly inhibited bacterial growth. This suggests that pomegranate peel not only reduces bacterial viability but may also possess components that attack various bacterial virulence factors.
Impact on Hydrophobicity and Virulence Factors
The study assessed the effect of plant extracts on the hydrophobicity of bacterial cells, finding that pomegranate and apple extracts altered the hydrophobic surface properties of K. pneumoniae. This alteration could reduce the bacteria's ability to adhere to host tissues, thereby lowering their virulence. Moreover, extracts from apple and lemon resulted in decreased hemagglutination ability, indicating their potential to disrupt bacterial adhesion mechanisms.
Histopathological Observations in Mice
An in vivo component of the study involved infecting mice with K. pneumoniae and treating them with pomegranate peel extract. Mice treated with the extract displayed no significant histopathological changes, contrasting with those infected without treatment, which exhibited acute cellular degeneration and damaged kidney structures. This suggests that pomegranate extract not only mitigates bacterial proliferation but may also prevent damage caused by K. pneumoniae infection.
In conclusion, the research highlights pomegranate peel extract's significant antibacterial and protective properties against Klebsiella pneumoniae, supporting its potential use as a natural alternative to traditional antibiotics in managing urinary tract infections. This underscores the importance of exploring plant-based solutions to combat antibiotic resistance and protect public health.
FAQ section (important questions/answers):
What was the aim of the study on plant extracts?
The study aimed to evaluate the antibacterial activity of four aqueous plant extracts (pomegranate, apple, lemon, and orange peel) against Klebsiella pneumoniae isolated from urinary tract infections, both in vitro and in vivo.
How did pomegranate extract affect Klebsiella pneumoniae?
Pomegranate extract exhibited the highest antibacterial activity, significantly reducing bacterial survival at concentrations of 1000 mg/mL and higher, and it prevented histopathological changes in infected mice kidneys.
What were the effects of apple and lemon extracts?
Apple extract reduced bacterial survival notably at 4000 mg/mL. Lemon extract's effect was slightly less effective compared to pomegranate and apple in inhibiting bacterial growth and virulence factors.
What virulence factors were studied in the research?
The study analyzed various virulence factors, including hydrophobicity, hemagglutination, curli expression, hemolysin production, and hypermucoviscosity of Klebsiella pneumoniae after exposure to the plant extracts.
What method was used for in vivo testing?
In vivo testing involved infecting laboratory mice with Klebsiella pneumoniae and then administering pomegranate peel extract, observing its protective effects and pathological changes in kidney tissues.
Why are plant extracts considered for treating infections?
Plant extracts possess antimicrobial properties that can combat antibiotic-resistant pathogens effectively. They are traditionally used in medicine, minimizing side effects while targeting harmful bacteria.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Effects of plant extracts on Klebsiella pneumonia in UTI cases.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Pomegranate:
Pomegranate refers to the fruit of the plant Punica granatum, known for its rich phytochemical content, including tannins and flavonoids. It exhibits notable antibacterial properties, particularly against opportunistic pathogens like Klebsiella pneumoniae. Pomegranate extracts have been studied for their potential therapeutic applications in treating infections, thus highlighting their medicinal relevance.
2) Lemon:
Lemon is the fruit from the Citrus limon plant, recognized for its high vitamin C and antioxidant content. In the context of antibacterial studies, lemon peels have demonstrated antimicrobial effects, contributing to the reduction of pathogenic bacteria like Klebsiella pneumoniae. Its inclusion in traditional medicine practices emphasizes its therapeutic potential.
3) Activity:
Activity in this context pertains to the biological responses generated by plant extracts against specific pathogens. The antibacterial activity of extracts from plants like pomegranate and lemon is crucial for understanding their potential use in treating infections caused by bacteria such as Klebsiella pneumoniae. It provides insights into their efficacy as natural antimicrobials.
4) Flavonoid:
Flavonoids are bioactive compounds found in many plants, including pomegranates and lemons, known for their antioxidant and antimicrobial properties. These compounds can interfere with bacterial metabolism and help prevent the attachment of pathogens to host tissues, making them significant in the development of phytochemical therapies for infections.
5) Antibiotic (Antibacterial):
Antibiotics are substances that kill or inhibit the growth of bacteria, often derived from microbial sources. The rise of antibiotic resistance necessitates the exploration of alternative treatments, such as plant extracts with natural antibacterial properties, which may offer effective solutions to combat bacterial infections, including those caused by Klebsiella pneumoniae.
6) Water:
Water serves as a solvent for extracting phytochemicals from plant materials in the preparation of plant extracts. Its role is vital in research examining the antimicrobial properties of these extracts, especially in the context of traditional medicine where water-based infusions or decoctions are commonly used to harness the healing potentials of plants.
7) Blood:
Blood is an essential bodily fluid that circulates nutrients, gases, and waste products. In the context of infections, understanding how bacteria like Klebsiella pneumoniae interact with blood components is crucial. The study investigates the effects of plant extracts on reducing pathogen load in blood, highlighting their pharmacological potential.
8) Medicine:
Medicine encompasses practices, substances, and methodologies employed for diagnosis, treatment, and prevention of diseases. The exploration of plant extracts, such as those derived from pomegranate and lemon, emphasizes the integration of traditional herbal remedies into modern medicine, possibly leading to novel therapeutic options against resistant bacterial infections.
9) Science (Scientific):
Science is the systematic study of the structure and behavior of the physical and natural world through observation and experimentation. The research discussed employs scientific methods to evaluate the antimicrobial properties of plant extracts, contributing to the evidence-based understanding of their efficacy in treating bacterial infections.
10) Drug:
A drug is a chemical substance used for medical treatment, whether to cure, prevent, or alleviate symptoms of disease. The findings pertaining to plant extracts inform the development of new natural drugs, suggesting an alternative route for therapeutic intervention, particularly against microbial infections resistant to conventional antibiotics.
11) Punica granatum:
Punica granatum is the scientific name for the pomegranate plant. Its extracts are noted for their rich content of bioactive compounds, which exhibit various health benefits, including antibacterial properties. Research into Punica granatum contributes to the understanding of natural remedies against pathogens like Klebsiella pneumoniae and highlights its medicinal importance.
12) Phytochemical:
Phytochemicals are bioactive compounds produced by plants, believed to be responsible for various health benefits and protective effects against diseases. The phytochemical profile of pomegranate and citrus peel extracts suggests their potential as natural antibacterial agents, offering insights into their efficacy in managing bacterial infections and promoting health.
13) Substance:
A substance is a type of matter with specific properties. In the context of this research, substance refers to the active ingredients or compounds in plant extracts that exert antibacterial effects against bacteria like Klebsiella pneumoniae. Understanding these substances is key for developing effective natural therapies.
14) Glass:
Glass is often used in laboratory settings for various experiments involving plant extracts and bacterial cultures. Lab glassware is crucial for preparing solutions, conducting reactions, and safely storing samples. Its relevance underscores the scientific processes utilized in studying the antimicrobial properties of natural extracts.
15) Diet:
Diet refers to the food and drink consumed by individuals, which can influence health outcomes. Including plant-based extracts known for their antibacterial properties in one's diet may provide natural defenses against infections, underscoring the interconnection between nutrition and prevention of bacterial infections, such as urinary tract infections.
16) Salt (Salty):
Salt is commonly used in biological experiments to assess the hydrophobicity of bacterial cell surfaces. In the context of this study, the presence of varying concentrations of salt helps determine how plant extracts influence bacterial characteristics, such as aggregation and virulence, providing insights into their antibacterial mechanisms.
17) Citrus medica:
Citrus medica, known as citron, is another fruit in the citrus family recognized for its potential health benefits and phytochemicals. Its extracts, similar to those of lemon and orange, may possess antimicrobial properties, contributing to the study's emphasis on the role of citrus fruits in traditional medicine and infection control.
18) Citrus limon:
Citrus limon is the scientific name for lemon. Its peel extracts are investigated for their antibacterial activity against pathogens like Klebsiella pneumoniae. Research into Citrus limon highlights its significance in traditional medicine and supports the potential for developing new natural antimicrobials derived from citrus fruits.
19) Discussion:
Discussion refers to the section of a research paper where the implications, interpretations, and significance of the study's findings are presented. In this study, the discussion provides insight into the efficacy of plant extracts, their potential as alternative treatments, and the relevance of their phytochemical content in combating bacterial infections.
20) Cancer:
Cancer is a disease characterized by uncontrolled cell growth and can be influenced by various factors, including diet and phytochemical exposure. While the study focuses on antibacterial properties, the relationship between phytochemicals in fruits like pomegranate and potential anticancer properties could be an additional area of research, linking health to natural products.
21) Garlic:
Garlic is another plant noted for its medicinal properties and potential antibacterial effects. Although not the focus of this study, garlic's inclusion in discussions around natural antibacterials highlights the broader interest in phytochemicals from diverse sources and their roles in health promotion and disease prevention.
22) Sharman (Śarma, Sarmā, Sarma, Sharma, Śarman):
Sharma likely refers to a researcher cited in studies associated with the antibacterial properties or phytochemicals of plants. This reference illustrates the contributions of various scientists to the field of medicinal plants, furthering our understanding of natural remedies for combating resistant bacterial infections.
23) Reason:
Reason signifies the justification or rationale behind the study. The reason for researching the antibacterial properties of plant extracts, such as pomegranate and citrus peels, stems from the increasing problem of antibiotic resistance, emphasizing the need for alternative treatments derived from natural sources to address bacterial infections.
24) Gupta (Guptā):
Gupta may refer to a researcher whose work is pertinent to the study's findings or methodology. Citing various studies by Gupta illustrates the collaborative nature of scientific research and the cumulative knowledge gained from multiple sources in understanding the efficacy of natural compounds against pathogens.
25) Sura (Surā, Sūra, Śūra, Shura, Sūrā):
Sura might refer to Sura Ihsan Abed Chabuck, the lead author of the study. This individual's contribution emphasizes the collaborative effort in scientific research, showcasing the importance of various authors in conducting and reporting studies that contribute to our understanding of natural antimicrobial agents and their therapeutic potential.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Effects of plant extracts on Klebsiella pneumonia in UTI cases.’. Further sources in the context of Science might help you critically compare this page with similair documents:
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