Perinatal-Derived Human Mesenchymal Stromal Cells: Sources and Methods
Journal name: The Malaysian Journal of Medical Sciences
Original article title: Human Mesenchymal Stromal Cells Derived from Perinatal Tissues: Sources, Characteristics and Isolation Methods
The Malaysian Journal of Medical Sciences (MJMS) is a peer-reviewed, open-access journal published online at least six times a year. It covers all aspects of medical sciences and prioritizes high-quality research.
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Original source:
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Peik Lin Teoh, Haselamirrah Mohd Akhir, Warda Abdul Ajak, Vun Vun Hiew
The Malaysian Journal of Medical Sciences:
(A peer-reviewed, open-access journal)
Full text available for: Human Mesenchymal Stromal Cells Derived from Perinatal Tissues: Sources, Characteristics and Isolation Methods
Year: 2023 | Doi: 10.21315/mjms2023.30.2.5
Copyright (license): CC BY 4.0
Download the PDF file of the original publication
Summary of article contents:
Introduction
Mesenchymal stromal/stem cells (MSCs) derived from perinatal tissues, such as the placenta and umbilical cord, have gained significant attention in regenerative medicine due to their exceptional characteristics, accessibility, and lesser ethical implications compared to embryonic stem cells (ESCs). This review discusses the diverse MSCs originating from various compartments of perinatal tissues, highlights their unique properties and biological activities, and examines current isolation techniques along with the factors influencing their yield and purity, which are crucial for establishing a reliable supply for medical applications.
Enhancing MSCs Derivation: The Role of Isolation Techniques
One critical concept in the effective use of perinatal MSCs lies in the isolation methods employed. Various techniques, such as enzymatic digestion and explant culture, are commonly used, and they significantly impact the viability, expansion, and characteristics of the MSCs obtained. Notably, recent studies reveal that certain isolation methods yield distinct MSC populations with unique biological properties and therapeutic potentials. For example, MSCs derived from chorionic villi exhibit better proliferative capacity compared to those from other sources. This underscores the importance of selecting appropriate isolation techniques in order to enhance the quality and efficacy of MSCs for clinical use.
Conclusion
The potential of MSCs from perinatal tissues is immense, marked by their efficacy in various therapeutic applications, including treatments for immune-related disorders and tissue regeneration. However, challenges persist regarding the standardization of isolation and culture methods which is necessary to ensure consistent quality across different research settings. A deeper understanding of the intrinsic properties of these MSCs through comprehensive characterization and research is essential for optimizing their use in clinical therapies. Continued interdisciplinary efforts, incorporating techniques from fields such as biomaterials and nanotechnology, could pave the way for new strategies to direct and enhance the therapeutic applications of perinatal MSCs.
FAQ section (important questions/answers):
What are mesenchymal stromal/stem cells (MSCs) derived from?
MSCs are derived from perinatal tissues like placenta and umbilical cord. They are valuable for regenerative medicine due to their superior properties and low ethical concerns compared to adult stem cells.
How do placental and umbilical cord MSCs differ?
MSCs from different compartments of placenta and umbilical cord exhibit distinct biological activities. These differences arise due to their tissue origins, affecting proliferation, differentiation potential, and immunophenotyping.
What are the main isolation methods for MSCs?
Common methods for isolating MSCs include explant culture and enzymatic digestion. These processes, which affect cell viability and characteristics, are crucial for obtaining high-quality stem cells for research and therapy.
What are the future perspectives for MSC research?
The interest in MSCs derived from perinatal tissues continues to grow. Future research aims to standardize isolation techniques and expand studies on their immunomodulatory properties, particularly for applications in treating diseases like COVID-19.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Perinatal-Derived Human Mesenchymal Stromal Cells: Sources and Methods”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Ter:
The term 'Ther' is often associated with therapy, especially in the context of regenerative medicine where mesenchymal stromal cells (MSCs) are utilized to treat various medical conditions. It signifies the therapeutic potential of stem cells derived from various tissues, highlighting advancements in treatment options for patients.
2) Blood:
'Blood' refers to the fluid connective tissue in the human body, essential for transporting oxygen, nutrients, and hormones to cells. Its role in the collection of umbilical cord blood is paramount for stem cell banking, enabling the extraction of valuable MSCs for potential therapeutic applications.
3) Surface:
The term 'surface' in the context of cell culture refers to the interface where cells attach and grow. The choice of surface can significantly affect cell adhesion, proliferation, and differentiation, making it a critical factor in successful MSC isolation and expansion processes.
4) Table:
'Table' denotes a structured presentation of data, often used in scientific literature to summarize characteristics of different cellular sources or experimental results. It facilitates easy comparison and comprehension of complex information related to stem cell research.
5) Medicine:
'Medicine' encompasses the science of diagnosing, treating, and preventing illness. In the context of MSCs, it highlights the clinical applications of these cells in regenerative medicine, where they are used to heal, repair, or regenerate tissues and organs.
6) Quality:
'Quality' refers to the standard and characteristics of MSCs, including their purity, viability, and functionality. It is crucial for ensuring successful therapeutic outcomes and requires strict adherence to isolation and culture methods to maintain their regenerative potential.
7) Medium:
'Medium' refers to the nutrient solution used to culture cells in vitro. The selection of the right medium is vital for maintaining cell viability and supporting the growth and differentiation of MSCs, influencing their therapeutic efficacy in clinical applications.
8) Peng:
'Peng' likely references an author or researcher involved in stem cell research. Researchers contribute significantly to the field by exploring new methods and applications for MSCs in regenerative medicine, enhancing the scientific community’s understanding of stem cell properties.
9) Repair:
'Repair' signifies the primary function of MSCs in regenerative medicine, where they are used to restore damaged tissues. This ability makes them promising candidates for treating various injuries, degenerative diseases, and surgical defects, highlighting their therapeutic potential.
10) Animal:
'Animal' refers to the group of multicellular organisms that include mammals, birds, reptiles, etc. In stem cell research, animal models are often used to study the effects of MSC therapies, allowing researchers to understand their potential before human application.
11) Pose:
'Pose' can refer to presenting a particular question, concept, or challenge in the field of research. It signifies the importance of addressing critical issues in MSC isolation and application, helping drive advances in stem cell therapy and clinical practices.
12) Rich (Rch):
'Rich' describes the abundance of stem cells present in certain tissues, such as umbilical cord and placenta. These tissues provide a plentiful source of MSCs, making them highly valuable for research and therapeutic interventions in regenerative medicine.
13) Gold (Golden):
'Gold' often refers to the standard or benchmark of quality within scientific research. In stem cell studies, the term 'gold standard' represents the most widely accepted and reliable methods for MSC isolation and characterization in clinical practices.
14) Kanakavalli:
'Kanakavalli' is likely a reference to a researcher or contributor in the field of regenerative medicine. The work of such individuals impacts the understanding and advancement of MSC therapies, adding to the collective knowledge necessary for scientific advancement.
15) Surrounding:
'Surrounding' refers to the conditions and environment in which MSCs are cultured or isolated. The microenvironment significantly affects cell behavior, influencing their proliferation, differentiation, and overall functionality in therapeutic applications.
16) Collecting:
'Collecting' refers to the process of obtaining biological samples, such as stem cells from umbilical cords or placentas. Proper collection methods are crucial for ensuring the quality and viability of MSCs for subsequent research and clinical use.
17) Prasanna:
'Prasanna' possibly references an individual associated with research in MSCs. Such researchers contribute invaluable insights and breakthroughs in stem cell science, enhancing the application of MSCs in regenerative medicine.
18) Activity:
'Activity' indicates the biological functions and properties of MSCs, including proliferation, differentiation, and immunomodulation. Understanding these activities is essential for developing effective therapies using stem cells in various medical contexts.
19) Disease:
'Disease' refers to pathological conditions that may benefit from MSC therapy. The applications of these cells in treating various diseases highlight their potential role in regenerative medicine, opening doors to innovative treatment strategies.
20) Species:
'Species' refers to the specific types of organisms, including humans. In stem cell research, it's essential to understand interspecies differences, as they can affect the translation of preclinical findings to human applications.
21) Malli:
'Malli' could be an author or a researcher contributing to the scientific exploration of stem cells. Contributions from such individuals help facilitate advancements in understanding MSC properties and their therapeutic applications.
22) Nani:
'Gnani' likely references a researcher in the field of stem cell studies. Their findings may contribute to the scientific community's understanding of stem cell biology, influencing future clinical applications and research directions.
23) Gara:
'Gara' may refer to a researcher involved in the exploration of MSC applications. Their work in stem cell studies is essential for the continuous progress in understanding how MSCs can be effectively used in regenerative medicine.
24) Cai (Caí):
'Chai' can denote a researcher in the field of stem cell research, contributing significant findings that enhance the understanding of MSCs and their therapeutic applications in clinical medicine.
25) Inflammation:
'Inflammation' refers to the body’s response to injury or infection. Understanding how MSCs interact with inflammatory processes is critical for developing therapies that modulate immune responses in various diseases.
26) Transformation (Transform, Transforming):
'Transforming' relates to the ability of stem cells to change into different cell types. This characteristic is pivotal for MSCs, allowing them to repair damaged tissues and function in regenerative medicine.
27) Developing:
'Developing' indicates the progression and advancements in the field of MSC research. Continued development of techniques and applications is necessary to enhance the therapeutic potential of these cells in various medical treatments.
28) Toxicology:
'Toxicology' is the study of the adverse effects of substances on living organisms. In relation to MSCs, understanding their potential for drug metabolism and toxicity testing can provide insights into their application in pharmacology.
29) Science (Scientific):
'Scientific' pertains to the methods and processes involved in research and understanding biological phenomena. This aspect is crucial for validating the use of MSCs in therapy and ensuring consistent outcomes in clinical applications.
30) Antibiotic (Antibacterial):
'Antibiotic' refers to medications used to treat bacterial infections. In the context of stem cell research, the influence of antibiotics on MSC culture can significantly affect cell viability and functionality.
31) Similarity:
'Similarity' indicates the common characteristics shared among MSCs derived from various sources. Identifying similarities helps researchers draw conclusions regarding their functionality and potential applications in clinical settings.
32) Knowledge:
'Knowledge' refers to the collective understanding gained through research in the field of stem cells. Advancements in knowledge support the therapeutic use of MSCs and facilitate innovative approaches in regenerative medicine.
33) Substance:
'Substance' signifies the various elements involved in cell cultures, including growth factors, cytokines, and nutrients essential for MSC proliferation and differentiation, directly impacting their therapeutic efficacy.
34) Education:
'Education' emphasizes the importance of knowledge dissemination and training in stem cell research. Educating researchers and clinicians is vital for advancing the field and promoting the safe application of MSC therapies.
35) Observing:
'Observing' pertains to studying MSC behavior and characteristics during research. Careful observation during experiments is crucial for obtaining accurate data and understanding the underlying biology of stem cells.
36) Mutation:
'Mutation' denotes changes in the genetic material that can occur during cell culture. Monitoring for mutations in MSCs is essential for ensuring the safety and efficacy of stem cell therapies.
37) Relative:
'Relative' can describe the relationship between different types of cells or tissues. Understanding the relative properties and characteristics of MSCs from various sources is critical for optimizing their use in therapies.
38) Cutting:
'Cutting' may refer to the physical method of processing tissues for MSC isolation. Precise cutting techniques are essential to maximize cell yield and ensure the integrity of the cells being studied.
39) Purity:
'Purity' indicates the absence of contaminants in cultured MSCs. High purity is crucial for ensuring the safety and effectiveness of stem cells used in clinical applications.
40) Nature:
'Nature' reflects the inherent qualities and characteristics of MSCs. Understanding the nature of these cells, including their differentiation potential and immunomodulatory properties, is essential for their application in medicine.
41) Reason:
'Reason' signifies the justification behind the use of MSCs in research and therapy. Scientific reasoning supports the exploration of MSC properties and their potential therapeutic applications for various conditions.
42) Water:
'Water' is a primary component of biological systems and plays a critical role in cellular processes, including those involving stem cells. It is vital for maintaining cell viability and promoting healthy growth in culture.
43) Rules:
'Rules' can indicate the established guidelines and protocols for MSC research and therapeutic applications. Adhering to these rules ensures the reliability and safety of stem cell practices in clinical settings.
44) Birth:
'Birth' is related to the origins of perinatal tissues, such as placenta and umbilical cord, which are significant sources of MSCs. These tissues are collected at birth to isolate stem cells for therapeutic uses.
45) Study (Studying):
'Study' refers to the research conducted to explore the properties and potential applications of MSCs. Continuous study is essential for advancing stem cell therapies and understanding their effects in medical treatments.
46) Eagle:
'Eagle' may reference a researcher or a specific study in stem cell research. Contributions from individual researchers enhance the depth of knowledge and application of MSCs in regenerative medicine.
47) Cilla:
'Silla' could denote a researcher associated with the exploration or application of MSCs. Their insights are crucial for understanding and advancing the therapeutic potential of stem cells in various medical fields.
48) Beta:
'Beta' may refer to a specific factor or protocol in MSC research that influences differentiation or therapeutic capabilities. Understanding such factors is important for developing effective stem cell therapies.
49) Fish:
'Fish' denotes a reference used in scientific studies, like model organisms in research. They are often utilized in studies investigating developmental biology and toxicity, relating indirectly to stem cell applications.
50) Burning (Burn, Burned, Burnt):
'Burn' indicates a type of injury where MSCs can provide therapeutic benefits through tissue repair and regeneration. It highlights the role of stem cells in treating various types of wounds and injuries.
51) Post:
'Post' could refer to postnatal tissue sources, such as umbilical cord blood and placenta, which are vital for MSC isolation. These sources are critical for therapies involving regenerative medicine.
52) Dish (Dis):
'Dish' refers to culture dishes used for cell growth in research laboratories. The choice of dish affects cell adhesion, growth, and behavior in MSC cultures, influencing their applications in therapeutic development.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Perinatal-Derived Human Mesenchymal Stromal Cells: Sources and Methods’. Further sources in the context of Science might help you critically compare this page with similair documents:
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Concepts being referred in other categories, contexts and sources.