Accelerated Senescence and Apoptosis in Rat Liver during Diabetes
Journal name: The Malaysian Journal of Medical Sciences
Original article title: Accelerated Senescence and Apoptosis in the Rat Liver during the Progression of Diabetic Complications
The Malaysian Journal of Medical Sciences (MJMS) is a peer-reviewed, open-access journal published online at least six times a year. It covers all aspects of medical sciences and prioritizes high-quality research.
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Ratih Yuniartha, Nur Arfian, Wiwit Ananda Wahyu Setyaningsih, Sagita Mega Sekar Kencana, Dwi Cahyani Ratna Sari
The Malaysian Journal of Medical Sciences:
(A peer-reviewed, open-access journal)
Full text available for: Accelerated Senescence and Apoptosis in the Rat Liver during the Progression of Diabetic Complications
Year: 2022 | Doi: 10.21315/mjms2022.29.6.5
Copyright (license): CC BY 4.0
Download the PDF file of the original publication
Summary of article contents:
Introduction
Diabetes mellitus (DM) is characterized by persistent hyperglycemia resulting from insulin deficiency or resistance. As of 2021, it affected approximately 537 million adults globally, a number projected to rise significantly by 2030. Chronic hyperglycemia leads to severe complications affecting various organs, particularly the liver. While the mechanisms underlying liver damage in diabetes are not fully understood, studies indicate a strong connection between oxidative stress and inflammation in the progression of liver injury in diabetic patients. This research aims to elucidate the pathological changes in the liver associated with long-term diabetes, as observed in a rat model.
Increased Apoptosis and Senescence in Diabetic Liver
One crucial finding of this study is the marked increase in apoptosis and cellular senescence in the livers of diabetic rats, which was closely associated with elevated mRNA expressions of key apoptotic markers such as p53 and caspase-3. The study revealed that prolonged hyperglycemia resulted in significant upregulation of the p53 protein, a critical regulator involved in cell cycle control and apoptosis. In conjunction, the expressions of senescence markers such as p21 and p16 further indicated that hyperglycemic conditions induce both premature senescence and apoptosis in liver cells. This synergistic relationship suggests that oxidative stress and prolonged hyperglycemia may significantly impair liver function by promoting cellular damage and death through biochemical pathways mediated by p53.
Conclusion
The findings highlight the detrimental impact of chronic hyperglycemia on liver health, emphasizing the roles of apoptosis and senescence in the associated pathological changes. The increased expression of p53, p21, and caspase-3 in the diabetic liver indicates a cascade of cellular responses to oxidative stress, ultimately leading to liver injury. Consequently, understanding these mechanisms sheds light on potential interventions that can mitigate liver complications in diabetic patients, suggesting that targeting oxidative stress and regulating apoptosis pathways may offer therapeutic benefits in preventing diabetes-related liver damage.
FAQ section (important questions/answers):
What is the main focus of this diabetes study?
The study aims to investigate the pathological changes and complications in the liver of diabetic rats due to prolonged hyperglycemia, emphasizing apoptosis and cellular senescence mechanisms involved.
How was diabetes induced in the rats for the study?
Diabetes was induced in male Sprague Dawley rats through a single intraperitoneal injection of streptozotocin (STZ) at a dose of 60 mg/kg body weight.
What significant changes were observed in the liver tissues?
In the liver tissues of diabetic rats, there were significant increases in apoptosis markers such as p53 and caspase-3, alongside increased cellular senescence as indicated by the rise in p16-positive cells.
What are the implications of oxidative stress in diabetes?
Oxidative stress from chronic hyperglycemia contributes to cellular damage, inflammation, and apoptosis, which may lead to severe complications, including liver injury, as observed in diabetic conditions.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Accelerated Senescence and Apoptosis in Rat Liver during Diabetes”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Table:
In the text, 'Table' refers to a structured presentation of data, typically found in scientific articles or studies. It organizes complex information, making it easier for readers to analyze results and compare findings. Tables often summarize experimental data, sample sizes, or results from statistical analyses, enhancing clarity and communication of research outcomes.
2) Death:
The term 'Death' is relevant in a medical and biological context, especially regarding apoptosis, which is programmed cell death. This process is critical for maintaining cellular health, especially in diseases like diabetes, where uncontrolled cell death may indicate pathological conditions, contributing to disease progression and affecting overall organ function.
3) Disease:
'Disease' signifies a pathological condition affecting an organism. In the context of the text, it highlights diabetes and its complications, illustrating how chronic hyperglycemia leads to organ dysfunction and diverse health issues, emphasizing the need for understanding disease mechanisms for effective treatment and management strategies.
4) Study (Studying):
The term 'Study' pertains to systematic research aimed at understanding complex biological processes, such as the impact of diabetes on liver health. In the text, it describes the methodologies and findings regarding long-term diabetes effects in rats, highlighting the importance of experimental research to inform clinical practices.
5) Activity:
'Activity' refers to the biological or enzymatic functions occurring within cells or organisms. In the context of the text, it often relates to antioxidant enzyme activity, apoptosis, or gene expression levels. Understanding these activities helps map cellular responses to stressors like hyperglycemia and their implications in diseases like diabetes.
6) Blood:
'Blood' signifies a vital fluid that plays a key role in transporting nutrients, gases, and waste products throughout the body. In the study context, blood glucose levels are crucial indicators of diabetes, influencing the research on how prolonged hyperglycemia affects organs and contributes to disease progression.
7) Inflammation:
'Inflammation' refers to the body’s immune response to injury or infection. In the text, it illustrates how chronic inflammation can exacerbate conditions like diabetes and liver damage. Understanding the inflammatory processes involved helps reveal mechanisms driving complications and inform therapeutic approaches to mitigate tissue damage.
8) Arrow:
'Arrow' may represent metaphorical paths in research, guiding findings and hypotheses towards specific conclusions or further inquiries. While not commonly a scientific term, it can symbolize directionality in data trends or the flow of research arguments, aiding comprehension of complex relationships in study outcomes.
9) Animal:
'Animal' pertains to the organism used in research, particularly in biomedical studies. The text references male Sprague Dawley rats as subjects in diabetes research. Animal models provide insights into disease mechanisms and therapy effects, enabling the exploration of hypotheses that inform human health and treatment approaches.
10) Post:
'Post' often indicates a temporal reference in research scenarios, such as 'post-injection' referring to the period after the administration of a treatment or substance. In the study context, it helps position observations within a timeline, crucial for understanding the effects of treatments administered during experimental procedures.
11) Toxicity:
'Toxicity' refers to the degree to which a substance can harm organisms. In the context of chronic hyperglycemia and diabetes, it highlights the detrimental effects of prolonged exposure to high glucose levels on cellular function and structure, emphasizing the need for understanding and mitigating toxic effects in metabolic diseases.
12) Relative:
'Relative' indicates a comparative relationship within data or findings. It can define how results or expressions relate to one another, essential in analyzing experimental outcomes. In the study context, relative expression levels of genes shed light on variability among groups, contributing to understanding underlying biological processes.
13) Species:
'Species' defines a group of living organisms classified together based on shared characteristics. In biomedical research, understanding species-specific responses, such as how diabetic models reflect human conditions, helps translate findings to broader applications, emphasizing the importance of selecting appropriate models for accurate and applicable research conclusions.
14) Cancer:
'Cancer' denotes a range of diseases involving abnormal growth of cells. In the study's broader context, understanding mechanisms such as cell apoptosis and senescence is critical for deciphering pathways underlying not only diabetes complications but also cancer development, revealing intersections between metabolic diseases and oncological research.
15) Nobar:
'Nobar' in the study context refers to authors contributing to the research. It highlights collaborations or specific individuals who may influence the study's design, implementation, or analysis. Recognizing contributions is crucial for crediting intellectual efforts and understanding perspectives that shape scientific inquiry.
16) Life:
'Life' encapsulates the biological processes that sustain living organisms. The study explores vital aspects of liver function and the effects of hyperglycemia on cellular health, tying into broader discussions about maintaining life quality amid chronic conditions like diabetes, illustrating the significance of metabolic health.
17) Accumulation (Accumulating, Accumulate):
'Accumulating' refers to the gradual build-up of substances or evidence over time. In the study context, it signifies the progressive nature of changes observed in diabetic conditions, stressing the importance of understanding how gradual processes impact liver and overall metabolic health.
18) Repair:
'Repair' signifies the biological processes that maintain or restore tissue integrity. In the context of organ damage due to chronic conditions like diabetes, understanding cellular repair mechanisms is crucial for developing treatments that enhance recovery and improve organ function, highlighting the dynamic nature of biological systems.
19) Bile:
'Bile' is a digestive fluid important for lipid metabolism. In the context of diabetes, understanding the relationships between bile production and metabolic disorders can reveal insights into gastrointestinal health and complications, contributing to a comprehensive view on the interconnectedness of metabolic processes and organ function.
20) Measurement:
'Measurement' refers to assessing characteristics or quantities in an experimental context. In the study, accurate measurement of gene expressions and biochemical markers is essential for validating findings and drawing scientific conclusions, underscoring the importance of methodology in research outcomes and data interpretation.
21) Medicine:
'Medicine' relates to the science of diagnosing, treating, and preventing diseases. The study informs medical knowledge regarding diabetes' effects on the liver, aiming to enhance interventions and understanding of diabetes complications, emphasizing the continuous need for research to advance medical practice and health strategies.
22) Citizen:
'Citizen' can symbolize the impact of scientific research on the public. In healthcare contexts, the knowledge produced by studies influences public health policies and individual health decisions. It underscores the significance of translating research findings into practical applications that enhance community well-being and disease management.
23) Science (Scientific):
'Science' represents the systematic pursuit of knowledge and understanding through observation and experimentation. The study embodies scientific investigation into diabetes and its effects on liver health, emphasizing the role of empirical evidence in advancing medical knowledge, influencing treatments, and shaping healthcare practices.
24) Mantha (Mamtha):
'Mantha' refers to an individual involved in the research, potentially as an author or contributor. Acknowledging individuals like Mantha in the scientific narrative highlights the collaborative nature of research efforts and recognizes the contributions of professionals in advancing understanding and exploration of complex medical issues.
25) Mishra (Misra):
'Mishra' denotes another contributor to the research study. Similar to other named individuals, recognizing Mishra's involvement emphasizes teamwork in scientific inquiry, reinforcing how collaborative efforts enrich understanding, methodologies, and insights in the study of diabetes and its implications on organ health.
26) Takara:
'Takara' represents a brand known for producing scientific research tools and reagents. References to Takara might pertain to specific materials or methods used in the study, highlighting the importance of quality and reliable tools in conducting rigorous scientific research and ensuring data validity.
27) Gupta:
'Gupta' indicates an individual associated with the study, likely an author or researcher. Identifying contributors like Gupta underscores the collaborative nature of scientific research while promoting transparency and acknowledgment of various expertise harnessed to deepen understanding of diabetes-related processes.
28) Rules:
'Rules' refer to established guidelines or principles guiding scientific research. In this context, they could imply ethical regulations and methodologies adhered to during the study. Understanding and following rules is crucial in ensuring valid, reproducible results and maintaining integrity in scientific inquiry.
29) Rati:
'Radhi' signifies a person involved in the study, potentially an author or an expert contributing to research efforts. Naming contributors like Radhi emphasizes collaboration in scientific inquiries, reinforcing how diverse expertise enhances the analysis of diseases and deepens insights into mechanisms underlying health conditions.
30) Mita:
'Midha' similarly refers to an individual involved in the research. Recognizing contributors like Midha highlights the importance of teamwork in scientific studies, underlining how collaboration across diverse disciplines enhances understanding of complex medical topics, such as the relationship between diabetes and liver function.
31) Giri:
'Giri' points to another contributor in the study. Acknowledging Giri reflects the collaborative spirit intrinsic to scientific research, emphasizing that pooling expertise from multiple professionals enhances the depth and breadth of findings related to diabetes and its health implications.
32) Vega:
'Vega' could signify a collaborator in the research study, reinforcing the concept of teamwork in scientific endeavors. Recognizing individuals like Vega emphasizes how collaboration across various domains of expertise contributes to thorough investigations into medical conditions and fosters advancements in treatment strategies.
33) Beta:
'Beta' can refer to beta cells in the pancreas, critical for insulin production. In the context of diabetes, understanding beta cell health and function is crucial for addressing metabolic disorders, highlighting their role in glucose homeostasis and the pathogenesis of diabetes-related complications.
34) Mari:
'Mari' appears as a contributor to the research narrative, emphasizing collaboration in research initiatives. Acknowledging contributors such as Mari reinforces the importance of teamwork in advancing knowledge of diabetes and associated disorders, showcasing diverse perspectives and expertise within scientific research.
35) Sho (So):
'Shao' indicates an individual likely related to the research effort. Recognition of contributors like Shao highlights the collaborative framework of scientific inquiry, signifying how combined expertise can enrich understanding and drive significant findings in studies related to diabetes and liver health.
36) Viru:
'Viru' potentially refers to a contributor or a subject area related to the research. Recognizing diverse contributors, including Viru, highlights the interdisciplinary aspect of research aimed at understanding diabetes mechanisms, reinforcing the necessity of collaboration and shared expertise in advancing medical knowledge.
37) Discussion:
'Discussion' signifies a crucial section of scientific writing where interpretations and implications of findings are analyzed. It contextualizes results within existing literature, providing insights and proposing future research directions. The discussion enhances the understanding of complex relationships, making it integral to scientific communication.
38) Lighting:
'Lighting' pertains to illumination, which may metaphorically represent clarity or insight provided by research findings. While not directly linked to the primary medical theme, it can symbolize the ‘shedding of light’ on complex topics, facilitating greater understanding and approachability of research results.
39) Account:
'Account' refers to reporting or describing findings. In the context of scientific studies, it can pertain to compiling results and interpretations. An accurate account ensures transparency and reliability in research communication, essential for advancing understanding and facilitating evidence-based practice in medicine.
40) Water:
'Water' is vital for life, influencing various physiological processes. In diabetes, it is crucial for kidney function and fluid balance. Understanding water metabolism and its relationship with blood sugar levels emphasizes metabolic complexities and informs management strategies for maintaining health in diabetic patients.
41) Ulcer:
'Ulcer' signifies a sore on an organ or tissue, often associated with chronic conditions like diabetes. Diabetic ulcers are significant clinical complications requiring attention; understanding their pathogenesis can inform prevention and treatment strategies, emphasizing the need for comprehensive care in managing diabetic patients.
42) Mada:
'Mada' appears as a personal identifier, likely a contributor to the study. Mentioning individuals like Mada highlights collaboration in research efforts, stressing the importance of diverse expertise in understanding the medical aspects of diabetes and enhancing knowledge pertaining to its complications.
43) Line:
'Line' can symbolize a boundary or connection within scientific data. In experimental research, lines often relate to pathways, signaling cascades, or even lineage in genetic studies, signifying how understanding the connections between data points or biological processes aids in unraveling complex disease mechanisms.
44) Male:
'Male' refers to the sex of animal subjects in the study, which is often relevant in biomedical research. Recognizing the specific sex of studied organisms helps delineate biological responses and implications in metabolic diseases like diabetes, underscoring the importance of sex-specific health considerations.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Accelerated Senescence and Apoptosis in Rat Liver during Diabetes’. Further sources in the context of Science might help you critically compare this page with similair documents:
Diabetes mellitus, Mode of action, Allicin, Oxidative stress, Animal model, Bile acid, Reactive oxygen species, Type 2 diabetes, Diabetic Nephropathy, Non Alcoholic Steatohepatitis, Oxidative damage, Apoptosis, Type II DM, Cellular senescence, Glutathione peroxidase, Superoxide dismutase, Reactive oxygen species (ROS), Programmed cell death, Type 1 diabetes, Total antioxidant capacity, Inflammatory cytokines, Superoxide anion, MRNA expression, Blood glucose, Reverse transcription–polymerase chain reaction, Diabetes mellitus type 2, Insulin deficiency, Kidney disease, High blood glucose, Energy Metabolism, Hyperglycaemia, Chronic hyperglycaemia, Histological structure, Diabetic Complication, Streptozotocin (STZ), Streptozotocin induced diabetes, Liver tissue, Liver Injury, Epstein-Barr Virus, DNA damage, Antioxidant defense, Antioxidant defense system, Glucose auto-oxidation, Antioxidant enzyme, Diabetes mellitus (DM), Caspase-3 activity, Caspase-3, Apoptotic marker, SOD Activity, Cell cycle arrest, Diabetic cardiomyopathy, Tumor suppressor, Cellular aging, Senescent cells, Replicative senescence, Apoptotic cell death, Immunohistochemistry, Cell death, Rat liver, P53, Antioxidant function, Hyperglycaemic condition, Diabetic Condition, Intrinsic apoptotic pathway, G1 cell cycle arrest, P53 gene, P53 protein, Macrovacular and microvascular complications, Antioxidant genes, High glucose, Apoptosis pathway, Diabetes duration, Endogenous antioxidant enzymes, Increased oxidative stress, Inflammatory pathway, Inflammatory marker, Oxidative stress condition, Endogenous stress, Senescence, Type II diabetic patients, Liver cells, Diabetic rat, Macrophage accumulation, ROS generation, Cyclin-dependent kinase inhibitor, Sprague Dawley rat, Target gene.
Concepts being referred in other categories, contexts and sources.