Tithonia diversifolia extract enhances insulin sensitivity in rats.
Journal name: The Malaysian Journal of Medical Sciences
Original article title: Standardised Ethanol Extract of Tithonia diversifolia (Hemsley) A Gray Leaves Improve Insulin Sensitivity and Increase Mitochondrial DNA Copy Numbers in Skeletal Muscles of Streptozotocin-Nicotinamide-Induced Rats
The Malaysian Journal of Medical Sciences (MJMS) is a peer-reviewed, open-access journal published online at least six times a year. It covers all aspects of medical sciences and prioritizes high-quality research.
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Rochmy Istikharah, Dwi Aris Agung Nugrahaningsih, Ahmad Hamim Sadewa, Mae Sri Hartati Wahyuningsih
The Malaysian Journal of Medical Sciences:
(A peer-reviewed, open-access journal)
Full text available for: Standardised Ethanol Extract of Tithonia diversifolia (Hemsley) A Gray Leaves Improve Insulin Sensitivity and Increase Mitochondrial DNA Copy Numbers in Skeletal Muscles of Streptozotocin-Nicotinamide-Induced Rats
Year: 2022 | Doi: 10.21315/mjms2022.29.3.5
Copyright (license): CC BY 4.0
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Summary of article contents:
Introduction
Insulin resistance is a major disorder seen in patients with type 2 diabetes mellitus (T2DM) and is often linked to impaired insulin signaling, particularly in muscle tissues. This study focused on the potential anti-diabetic effects of Tithonia diversifolia (Hemsley) A Gray extracts, evaluating their influence on insulin resistance and mitochondrial DNA (mtDNA) copy number. The research was driven by the hypothesis that increasing mtDNA could improve insulin sensitivity, providing a possible therapeutic avenue for T2DM management.
Enhancement of Mitochondrial DNA Content
The findings demonstrated that treatment with T. diversifolia extracts led to significant improvements in fasting plasma glucose levels and insulin resistance as measured by the Homeostatic Model Assessment for Insulin Resistance (HOMA-IR). Notably, the mtDNA content in skeletal muscle, specifically the soleus muscle, showed remarkable enhancement—up to threefold with a 100 mg/kg extract dose, compared to the diabetic control group. This increase likely corresponds with improved mitochondrial function and metabolic health, indicating a potential mechanism through which T. diversifolia exerts its anti-diabetic properties.
Conclusion
In conclusion, the study indicates that Tithonia diversifolia extracts have a promising potential as an anti-diabetic agent, effectively improving insulin sensitivity and increasing mtDNA copy number in skeletal muscles. These findings support the need for further research to unravel the precise mechanisms by which T. diversifolia enhances mitochondrial biogenesis and impacts insulin resistance, thereby offering valuable insights for developing treatments for T2DM.
FAQ section (important questions/answers):
What is the focus of the study on Tithonia diversifolia?
The study investigates the anti-diabetic activity of standardized extracts from Tithonia diversifolia leaves, particularly their effects on insulin resistance and mitochondrial DNA (mtDNA) copy number in diabetic models.
How was the extract of Tithonia diversifolia prepared?
The extract was prepared using an ultrasound-assisted method at 20 kHz and 40 °C for 30 minutes, followed by filtration and concentration using a rotary evaporator. It was standardized using Tagitinin C.
What results were found regarding blood glucose and insulin levels?
All treatment groups, including those receiving different doses of extract, showed significant improvements in blood glucose levels and insulin resistance as measured by the HOMA-IR score after 28 days of treatment.
What effect did Tithonia diversifolia have on mtDNA copy number?
Significant increases in mtDNA copy number were observed, with the most pronounced effects at a dose of 100 mg/kg, leading to up to three times more mtDNA in soleus muscles compared to diabetic controls.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Tithonia diversifolia extract enhances insulin sensitivity in rats.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Table:
A table presents organized data for easy reference, making comparisons clear and analysis more straightforward. In scientific studies, tables are essential for summarizing results effectively, allowing researchers to communicate findings efficiently to other scientists, thus facilitating understanding and further investigation in the respective fields.
2) Homa:
HOMA (Homeostatic Model Assessment) is a method used to estimate insulin resistance and beta-cell function from fasting plasma glucose and insulin levels. It is significant in diabetes research as it helps assess metabolic state and guide treatment strategies in understanding type 2 diabetes pathophysiology and management.
3) Study (Studying):
A study is a systematic investigation aimed at discovering or interpreting knowledge about a subject. In scientific research, studies provide evidence-based insights that inform clinical practices, policy-making, and further research directions, contributing significantly to advancements in healthcare, pharmacology, and disease management.
4) Activity:
Activity in a scientific context refers to the effects or functions of a substance or process. For example, in pharmacological research, the biological activity of a drug or compound reflects its capability to produce a therapeutic effect, which is crucial for developing effective medications.
5) Blood:
Blood is a vital fluid in the body that transports oxygen, nutrients, hormones, and waste products. In medical research, blood analysis is essential for diagnosing diseases, understanding metabolic processes, and evaluating various health conditions, particularly in studies related to diabetes and insulin resistance.
6) Flavonoid:
Flavonoids are a diverse group of phytonutrients found in many fruits, vegetables, and beverages. They possess antioxidant properties and are believed to provide several health benefits, including anti-inflammatory and immune-boosting effects. Research into flavonoids is essential for understanding their potential role in disease prevention and treatment.
7) Quality:
Quality refers to the overall standard of something, evaluated based on certain criteria. In pharmacology and medicine, the quality of extracts, drugs, or medical products is crucial for ensuring their efficacy, safety, and reliability in clinical practice, necessitating rigorous testing and regulation.
8) Animal:
Animals are often used in biomedical research to model human diseases, enabling scientists to study biological processes and test potential therapies. Their responses to treatments can provide insight into human health, disease mechanisms, and the effectiveness of pharmacological agents used in medicine.
9) Water:
Water is essential for all known forms of life and plays numerous roles in biological processes, including temperature regulation and nutrient transport. In scientific studies, assessing water content is vital for determining the physicochemical properties of substances, especially in the characterization of herbal extracts and pharmaceuticals.
10) Rules:
Rules in scientific research refer to established guidelines and protocols that govern experimental design, data analysis, and ethical considerations. Adherence to these rules ensures the integrity, reproducibility, and validity of research findings, providing a foundation for credible scientific knowledge advancement.
11) Measurement:
Measurement is a fundamental aspect of scientific research, involving the quantitative assessment of various parameters, such as concentrations of substances, biological responses, or physical properties. Accurate measurements are critical for ensuring the reliability and validity of research findings, leading to meaningful conclusions.
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Medicine is the science and practice of diagnosing, treating, and preventing diseases. It encompasses a wide range of therapeutic approaches and interventions. Ongoing research in the field of medicine is essential for advancing healthcare, improving treatment outcomes, and understanding human health complexities.
13) Food:
Food is any substance consumed to provide nutritional support for the body. In the context of pharmacology and health, the study of food can reveal the relationships between diet, nutrition, health, and disease prevention. Dietary components can affect metabolic processes and pharmacological activities.
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Pharmacological relates to the study of drugs and their effects on biological systems. Understanding pharmacological principles is fundamental for developing new medications, determining therapeutic actions, and ensuring safe and effective treatment options for various health conditions, including diseases like diabetes.
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Antibacterial agents are substances that inhibit bacterial growth or kill bacteria. They play a crucial role in treating infections and preventing the spread of bacterial diseases. Research on antibacterial compounds is particularly relevant in the development of new treatments against resistant bacterial strains in medicine.
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Phytochemicals are naturally occurring compounds in plants that contribute to their color, flavor, and disease resistance. Many phytochemicals exhibit biological activities, including antioxidant, anti-inflammatory, and anticancer properties, making them a focus of nutritional research and herbal medicine applications.
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Calculation in scientific research involves mathematical methods used to analyze data, determine effects, and draw conclusions. Accurate calculations are essential for interpreting results, particularly in studies evaluating the efficacy of treatments or therapies in medicine and pharmacology.
18) Leonardo:
Leonardo may refer to a contributor's name in a study, suggesting their involvement in research or publication. Recognizing contributors is essential in academia as it attributes work and insights to the individuals who advance knowledge in specific fields like medicine or pharmacology.
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Kanchan may refer to an individual, likely a co-author or researcher in a scientific study. The recognition of individual contributions is important in academia, as it highlights collaborative efforts, and collaborations often drive innovation and discoveries in researching health-related topics.
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In a scientific research context, destiny could be metaphorically used to discuss the inherent outcomes or future direction of a study based on current findings. It reflects the potential impact of research discoveries on future treatment practices, health sciences, and patients' health outcomes.
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Disease is a pathological condition of a living organism that results from various factors, including genetic, environmental, or infectious agents. Understanding diseases is fundamental in medical research as it informs prevention, treatment strategies, and the development of effective pharmacological solutions.
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Aureus often refers to Staphylococcus aureus, a type of bacteria that can cause various infections in humans. Due to its relevance in clinical settings, understanding its behavior, resistance mechanisms, and pathogenicity is significant for developing effective antibacterial treatments in modern medicine.
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Rana could refer to another contributor's name associated with the study, indicating their role in research, data analysis, or interpretation. Citing all contributors is essential for transparency and credit in academic publications, emphasizing teamwork in advancing scientific knowledge.
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26) Post:
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A drug is a substance used to diagnose, treat, or prevent disease. In medical research, understanding the properties, efficacy, and safety of drugs is vital for developing new therapies. Pharmacology focuses on drug interactions, mechanisms of action, and therapeutic outcomes.
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Peer generally refers to a colleague or fellow researcher in scientific contexts. Peer review is an essential quality control process, ensuring research validity and reliability before publication. It also promotes academic integrity, enhances credibility, and fosters collaboration within the scientific community.
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Pharmacology is the branch of medicine that focuses on drug actions, their effects on biological systems, and therapeutic uses. Understanding pharmacological principles is crucial for drug development, providing insights into efficacy, safety, dosing, and mechanisms of action related to various health conditions.
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Roman may refer to historical or cultural contexts influencing scientific study or methodology. It could also allude to contributions or studies related to Roman history in pharmacology, suggesting the historical legacy of medicinal practices continuing to shape modern healthcare and drug development.
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36) Beta:
Beta often refers to beta cells in the pancreas, responsible for producing insulin. Understanding their function and responsiveness is pivotal in diabetes research, as dysfunction in beta cells leads to insulin resistance and contributes to the pathogenesis of type 2 diabetes.
37) Cage:
Cage refers to the housing used for laboratory animals during research studies. Proper design and care of cages are crucial for animal welfare and ensuring reliable study outcomes, as environmental factors can influence physiological responses that are critical in medical research.
38) Male:
Male pertains to one of the biological sexes, which can play a role in the physiological responses observed in animal studies. Understanding sex differences is essential in biomedical research as it impacts drug efficacy, disease progression, and overall health outcomes.
Other Health Sciences Concepts:
Discover the significance of concepts within the article: ‘Tithonia diversifolia extract enhances insulin sensitivity in rats.’. Further sources in the context of Health Sciences might help you critically compare this page with similair documents:
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