Characterization of Multidrug Pseudomonas aeruginosa Strains from Wounds

| Posted in: Science

Journal name: The Malaysian Journal of Medical Sciences
Original article title: Characterisation of the Prevailing Multidrug Pseudomonas aeruginosa Strains from Surgical Wound Using 16S rRNA Sequencing Technique
The Malaysian Journal of Medical Sciences (MJMS) is a peer-reviewed, open-access journal published online at least six times a year. It covers all aspects of medical sciences and prioritizes high-quality research.
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Original source:

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Author:

Osagie Aibuedefe Eremwanarue, Stanley Udogadi Nwawuba, Olalekan Hakeem Shittu


The Malaysian Journal of Medical Sciences:

(A peer-reviewed, open-access journal)

Full text available for: Characterisation of the Prevailing Multidrug Pseudomonas aeruginosa Strains from Surgical Wound Using 16S rRNA Sequencing Technique

Year: 2021 | Doi: 10.21315/mjms2021.28.4.5

Copyright (license): CC BY 4.0


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Summary of article contents:

Introduction

Pseudomonas aeruginosa is a prominent pathogen associated with hospital-acquired surgical wound infections, characterized by both innate and acquired resistance to various antimicrobials. This bacterium presents a significant challenge in clinical settings, as it is capable of causing severe infections particularly in immunocompromised patients and those with post-operative wounds. The prevalence of antibiotic resistance among P. aeruginosa strains is rising globally, necessitating effective diagnostic methods for timely identification and treatment to mitigate morbidity and mortality rates associated with surgical wound infections.

Molecular Identification and Antibiotic Resistance

In this study, a total of 284 surgical wound swabs were collected from two government hospitals in Benin City, Nigeria, and analyzed for P. aeruginosa strains. The molecular identification utilizing polymerase chain reaction (PCR) showed that a significant percentage of isolated strains were specifically identified as Pseudomonas spp., with the dominant strain being identified as H25883. The study revealed alarming rates of multi-drug resistance (MDR) among the isolates, with the highest resistance reported against ceftazidime in one hospital and nitrofurantoin in the other. This highlights the need for molecular techniques such as 16S rRNA gene sequencing, which proved more reliable than phenotypic methods, for accurate identification of bacterial strains.

Conclusion

The findings of this research underscore the importance of implementing advanced molecular techniques for the identification of P. aeruginosa, given its high rates of antibiotic resistance and the potential for misidentification through conventional methods. Early and accurate identification of this pathogen is crucial for improving therapeutic outcomes and controlling surgical wound infections. Continuous monitoring and further studies are needed to explore better management strategies and to reinforce infection control measures in hospital settings to combat the growing issue of multi-drug resistant P. aeruginosa.

FAQ section (important questions/answers):

What is the significance of Pseudomonas aeruginosa in surgical wound infections?

Pseudomonas aeruginosa is a common pathogen in hospital-acquired surgical wound infections. It is known for its high resistance to multiple antibiotics, making it a significant challenge in clinical settings for effective treatment.

How were the Pseudomonas spp. isolated in the study?

The study isolated Pseudomonas spp. from 284 surgical wound swabs collected from two hospitals. The isolates were screened using the disk diffusion method with eight different antibiotics to assess their resistance patterns.

What methods were used for identifying Pseudomonas aeruginosa strains?

Identification of Pseudomonas aeruginosa strains was carried out using polymerase chain reaction (PCR) techniques and 16S rRNA sequencing. This molecular characterization provided more reliable identification than traditional phenotypic methods.

What were the results regarding antibiotic resistance in Pseudomonas spp.?

The study found that 62.9% of isolates from one hospital and 57.1% from the other were multidrug-resistant. Ceftazidime showed the highest resistance rates among the tested antibiotics in Pseudomonas isolates.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Characterization of Multidrug Pseudomonas aeruginosa Strains from Wounds”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Table:
A table organizes data in a structured way, allowing for easy comparison and analysis. In research papers, tables summarize findings such as the distribution of bacterial species or their antibiotic resistance profiles. This visual representation enhances clarity and aids readers in quickly understanding complex results.

2) Antibiotic (Antibacterial):
Antibiotics are medications used to treat bacterial infections by inhibiting the growth of bacteria or killing them outright. The study of antibiotics includes their effectiveness against strains like Pseudomonas aeruginosa, particularly concerning resistance patterns. Understanding antibiotic action is crucial for effective treatment protocols in clinical settings.

3) Study (Studying):
A study investigates specific questions or hypotheses, using systematic methods to gather and analyze data. In medical and microbiological research, studies examine prevalence rates of infections, resistance patterns, and the effectiveness of diagnostic techniques. Results from such studies inform clinical practice and guide treatment strategies.

4) Post:
In a medical context, 'post' refers to the period following surgery, during which patients are vulnerable to infections. The study of post-operative infections seeks to understand the causative agents, such as specific bacteria, and to develop effective prevention and management strategies to reduce morbidity and mortality.

5) Species:
Species is a fundamental biological classification that groups organisms capable of interbreeding. In microbiology, identifying bacterial species is crucial for diagnosis and treatment of infections. This study categorizes isolated pathogens from surgical wounds, such as Pseudomonas aeruginosa, enhancing our understanding of their impact on human health.

6) Tree:
A phylogenetic tree visually represents evolutionary relationships among organisms, illustrating how species diverged over time. In the context of microbiology, tree diagrams show the genetic connections among bacterial strains, aiding in the identification of pathogenic relationships and the evolutionary history of multidrug-resistant species.

7) Relative:
In biological studies, 'relative' often refers to the comparison of different organisms concerning their genetic or phenotypic traits. Understanding the relative differences among bacterial strains, such as in their antibiotic resistance mechanisms, is essential for developing effective treatments and understanding their epidemiology.

8) Teaching:
Teaching involves imparting knowledge and skills, often within academic or clinical settings. In medical education, teaching about microbiology, infection control, and the management of antibiotic resistance is vital for training healthcare professionals, thereby improving patient outcomes and reducing the prevalence of hospital-acquired infections.

9) Water:
Water is essential for all forms of life and serves various roles in biological systems. In healthcare, hospital water systems can become sources of pathogens, including Pseudomonas aeruginosa, often contributing to nosocomial infections. Understanding water's role emphasizes the importance of sanitation in healthcare environments.

10) Rules:
Rules refer to established guidelines or standards used in various fields. In clinical microbiology, adherence to guidelines when performing susceptibility testing ensures standardization across laboratories, enhancing the reliability of results. This is crucial for accurate diagnostics and the effective treatment of infections.

11) Cina:
China refers to the country, which is relevant in research on infectious diseases as emerging resistance patterns often tracked in specific geographic locations, inform global health trends. Studies in China regarding Pseudomonas aeruginosa enhance understanding of the genetic diversity of strains and their response to antibiotics.

12) Drug:
Drugs are substances used to diagnose, cure, or prevent diseases. In microbiology, the study focuses on antibiotics and their efficacy against bacterial infections. Drug resistance is a significant concern, particularly with multidrug-resistant strains, necessitating ongoing research into new therapeutic options and treatment protocols.

13) Disease:
Disease refers to a pathological condition of a bodily part or system resulting from various factors, including infection. Studying diseases caused by opportunistic pathogens like Pseudomonas aeruginosa helps in understanding the mechanisms of infection, treatment failures, and the development of effective preventive measures in clinical settings.

14) Aureus:
Staphylococcus aureus is a common bacterium associated with various infections, including surgical site infections. Its relevance in the study emphasizes the need to understand comparative resistance patterns between S. aureus and other pathogens, such as Pseudomonas aeruginosa, to guide effective treatment strategies for managing bacterial infections.

15) Genu:
Genu refers to a genus in biological classification, representing a group of species that share common characteristics. Accurate identification of the genus, such as Pseudomonas, is essential for understanding the biology and pathogenic potential of specific bacteria, which is critically important for appropriate treatment and infection control.

16) Purification:
Purification involves the processes designed to isolate a specific substance from a mixture. In microbiological studies, purification techniques are essential for extracting DNA from bacteria, which is necessary for molecular identification, such as determining the presence of Pseudomonas aeruginosa in clinical samples.

17) Transformation (Transform, Transforming):
Transforming refers to significant changes or advancements in a system or methodology. In the context of microbiology and clinical practice, transforming diagnostic techniques via molecular methods enhances the accuracy and speed of identifying pathogens, leading to better patient care and informing treatment decisions in infected individuals.

18) Performance:
Performance in a medical context often relates to the effectiveness and accuracy of diagnostic tests or treatments. Assessing the performance of microbiological tests, particularly for identifying drug-resistant strains, is crucial for ensuring appropriate patient management and improving outcomes in infections caused by pathogens such as Pseudomonas aeruginosa.

19) Dakshayani (Daksayani):
Dakshayani is a surname mentioned in research highlighting specific studies or contributions in microbiology. Recognition of researchers contributes to understanding the collaborative efforts in studying microbial resistance patterns or disease prevalence, fostering developments in treatment methodologies and public health initiatives.

20) Evolution:
Evolution refers to the process by which organisms change over time through genetic variation and natural selection. Understanding the evolution of antibiotic resistance in bacteria like Pseudomonas aeruginosa provides insight into how these pathogens adapt to treatments, underscoring the need for novel strategies to combat resistant strains.

21) Medicine:
Medicine is the field dedicated to the prevention, diagnosis, and treatment of illness. The study of microbiological pathogens, their resistance mechanisms, and their impact on human health is integral to medicine as it informs clinical practices, enhances therapeutic approaches, and ultimately aims to improve patient outcomes.

22) Science (Scientific):
Science encompasses systematic study and understanding of the natural world through observation and experimentation. In medical microbiology, scientific research focuses on pathogenic bacteria, disease mechanisms, and treatment approaches, fostering innovations that advance healthcare practices and address challenges such as antibiotic resistance.

23) Sharman (Sarma, Sharma, Sarman):
Sharma is a surname that references an individual contributor to scientific literature or research findings. Recognizing individual contributors is vital, as it acknowledges the collaborative nature of scientific inquiry and the importance of diverse expertise in advancing knowledge, particularly in fields like microbiology and infectious diseases.

24) Nayak:
Nayak is a surname associated with research contributions in microbiology or related fields. The work of individuals with this surname in conducting studies on pathogens, antibiotic resistance, or clinical practices highlights the importance of collaboration and various perspectives in advancing scientific understanding and improving health outcomes.

25) Jang:
Jang is a surname referenced in scientific literature, identifying contributors to research, particularly in medical microbiology. Each researcher's work is part of a broader collective effort to study bacterial infections, resistance patterns, and effective treatments, which is essential for improving clinical outcomes for patients with infections.

26) Peng:
Peng, a surname found in research studies, often signifies contributions to understanding microbial pathogens and antibiotic resistance. The research conducted by individuals identified with this surname enhances collective knowledge, particularly on the transmission dynamics and treatment challenges presented by organisms like Pseudomonas aeruginosa.

27) Vina:
Viña is a surname that indicates individual contributions to the field of microbiology or medicine. The studies associated with researchers of this name often focus on infectious pathogens, contributing valuable insights into treatment protocols and improving public health measures through understanding resistance patterns among bacteria.

28) Pose:
Pose typically refers to the potential or threat that a particular organism or condition represents. In microbiology, the pose of multidrug-resistant pathogens like Pseudomonas aeruginosa emphasizes the urgent need for effective treatment strategies and public health initiatives to mitigate their impact on healthcare settings.

29) Burning (Burn, Burned, Burnt):
Burn refers to a type of wound that is particularly susceptible to infection, including from bacteria such as Pseudomonas aeruginosa. Understanding the dynamics of burn wounds and their healing processes is crucial in clinical practice to prevent infections and manage complications associated with these injuries.

30) Chan:
Chan is a surname associated with scientific contributions, particularly in microbiology and healthcare research. The work of individuals with this name often focuses on understanding bacterial pathogenicity and antibiotic resistance, which is vital for developing effective public health policies and clinical treatment strategies.

31) Inflammation:
Inflammation is a biological response to harmful stimuli, leading to redness, swelling, and pain. In the context of infections, inflammation indicates the body's immune response to pathogens like Pseudomonas aeruginosa. Understanding the mechanisms of inflammation helps in developing therapies to manage infection-related complications.

32) Discussion:
Discussion in a research paper interprets findings and places them within the context of existing knowledge. It allows for critical analysis and synthesis of data regarding microbial resistance, treatment efficacy, and implications for clinical practice, facilitating the advancement of scientific understanding and evidence-based healthcare.

33) Dressing:
Dressing refers to the material applied to wounds for protection and healing. In post-operative care, the choice of dressing can influence infection rates, particularly from opportunistic pathogens like Pseudomonas aeruginosa. Understanding the impact of different dressings is vital for infection control in surgical settings.

34) Nature:
Nature encompasses the physical world and its phenomena, including organisms, ecosystems, and biological processes. In medical microbiology, understanding the nature of pathogens, their habitats, and interactions with hosts is essential for developing effective prevention and treatment strategies for infectious diseases.

35) Animal:
Animal refers to a kingdom of living organisms, often studied in biology for insights into physiology, behavior, and ecology. In microbiology, animal models are frequently utilized to understand infection mechanisms and the efficacy of treatments, aiding researchers in the development of antibacterial therapies for human health.

36) Death:
Death signifies the cessation of biological functions that sustain a living organism, often resulting from severe infections, including those caused by multidrug-resistant bacteria. Understanding the relationships between infection complications and mortality rates is crucial for improving patient management and developing effective treatment strategies.

37) Blood:
Blood is a vital fluid in living organisms that transports oxygen, nutrients, and waste products. In the context of infections, the presence of bacteria like Pseudomonas aeruginosa in the bloodstream can lead to severe complications like sepsis, emphasizing the need for rapid diagnosis and effective treatment protocols.

38) Musha (Musa, Musá):
Musa is a surname that suggests contributions to scientific literature, particularly in studies surrounding infectious diseases and microbial pathogens. Recognizing the contributions of researchers bearing this surname emphasizes the importance of diverse insights and expertise in addressing complex health challenges.

39) Sign:
Sign refers to an indicator or symptom of a condition or disease. In microbiology, signs may include laboratory results indicating infection presence or resistance patterns among bacteria, highlighting the need for accurate diagnostics and informed treatment decisions in patient care.

40) Gold (Golden):
Gold is often associated with high standards or benchmarks in various contexts. In microbiology, PCR techniques and DNA sequencing are considered 'gold standard' methods for identifying bacterial strains due to their high sensitivity and specificity, establishing reliable means for diagnosis and research validation.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Characterization of Multidrug Pseudomonas aeruginosa Strains from Wounds’. Further sources in the context of Science might help you critically compare this page with similair documents:

Hospital equipment, Wound infection, Ethical committee, Clinical Practice, Antimicrobial Resistance, Pseudomonas aeruginosa, Gram negative bacteria, Antibiotic resistance, Polymerase Chain Reaction (PCR), Surgical Site, Early identification, Surgical site infection, P. aeruginosa, Hospital Environment, Bacterial isolate, PCR amplification, Multidrug-resistant, 16S rRNA sequencing, Nosocomial infection, Phylogenetic tree, Phylogenetic analysis, Clinical isolate, Multiple Drug Resistant (MDR), Antibiotic sensitivity, PCR technique, Pseudomonas species, Pseudomonas spp., Antibiotic sensitivity pattern, Hospital setting, Disk diffusion method, 16S rRNA gene sequencing, Bacterial identification, Nosocomial pathogen, Antibiotic Susceptibility Profile, Genomic DNA Extraction, Surgical wound infection, PCR assay, Phenotypic method, Bacterial taxonomy, 16S rRNA analysis, Morphological and biochemical tests, Multiple drug resistant, Medical microbiology, Antibiotic screening, 16S rRNA gene, Post-operative morbidity, P. aeruginosa Strain, Bacterial phylogeny.

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