Lasiodiplodia pseudotheobromae extract inhibits MRSA and biofilms.
Journal name: The Malaysian Journal of Medical Sciences
Original article title: Fungal Extract of Lasiodiplodia pseudotheobromae IBRL OS-64 Inhibits the Growth of Skin Pathogenic Bacterium and Attenuates Biofilms of Methicillin-Resistant Staphylococcus aureus
The Malaysian Journal of Medical Sciences (MJMS) is a peer-reviewed, open-access journal published online at least six times a year. It covers all aspects of medical sciences and prioritizes high-quality research.
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Mohd Taufiq Mat Jalil, Darah Ibrahim
The Malaysian Journal of Medical Sciences:
(A peer-reviewed, open-access journal)
Full text available for: Fungal Extract of Lasiodiplodia pseudotheobromae IBRL OS-64 Inhibits the Growth of Skin Pathogenic Bacterium and Attenuates Biofilms of Methicillin-Resistant Staphylococcus aureus
Year: 2021 | Doi: 10.21315/mjms2021.28.4.4
Copyright (license): CC BY 4.0
Download the PDF file of the original publication
Summary of article contents:
Introduction
The emergence of multidrug-resistant pathogens, particularly methicillin-resistant Staphylococcus aureus (MRSA), presents significant challenges in clinical settings due to their ability to form biofilms, which protect them against both antibiotic treatment and the immune response. This study focuses on the ethyl acetate extract of the endophytic fungus Lasiodiplodia pseudotheobromae (L. pseudotheobromae) IBRL OS-64, isolated from the medicinal plant Ocimum sanctum. The aim is to investigate its antibacterial and anti-biofilm properties against MRSA, given the urgent need for effective treatments against resistant bacterial strains.
Summary of One Important Concept: Anti-Biofilm Activity
The study found that the ethyl acetate extract of L. pseudotheobromae exhibited significant anti-biofilm activity, demonstrating an impressive ability to inhibit the formation of both initial and pre-formed biofilms of MRSA. The extract diminished the initial biofilm formation by approximately 80.82% and reduced the viability of pre-formed biofilms by 61.39%. Microscopic analyses indicated severe damage to bacterial cells upon exposure to the extract, including cell shrinkage, breakage, and membrane disruption, which contribute to its efficacy. The findings highlight that initial biofilms are more sensitive to the extract compared to established biofilms, underscoring the importance of early intervention in managing bacterial infections.
Conclusion
In conclusion, this research demonstrates that the ethyl acetate extract of L. pseudotheobromae IBRL OS-64 possesses notable antibacterial and anti-biofilm properties against MRSA, with a particular strength in preventing initial biofilm formation. The results suggest that this extract could serve as a promising candidate for developing new therapeutic strategies against multi-drug resistant bacteria. Further investigation into the mechanisms of action is warranted to fully understand how the extract exerts its effects on bacterial cell integrity and biofilm formation.
FAQ section (important questions/answers):
What is the primary focus of the study on L. pseudotheobromae?
The study aims to evaluate the effects of the ethyl acetate extract of Lasiodiplodia pseudotheobromae on the growth of methicillin-resistant Staphylococcus aureus (MRSA) and its ability to inhibit biofilm formation.
How was the anti-biofilm activity of the extract tested?
Anti-biofilm activity was tested using broth microdilution and semi-quantitative adherence assays, along with microscopy to observe the effects on MRSA biofilm formation.
What were the main findings regarding the effects on MRSA?
The extract significantly inhibited MRSA growth and reduced biofilm formation, showing an 80.82% reduction in initial biofilms and a 61.39% reduction in pre-formed biofilms.
What mechanisms might explain the extract's anti-biofilm activity?
The extract may disrupt cell membrane permeability and cause cell lysis. It operates effectively against initial biofilm formation while pre-formed biofilms are more resistant, indicating the complexity of biofilm defenses.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Lasiodiplodia pseudotheobromae extract inhibits MRSA and biofilms.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Table:
A table in scientific publications displays data succinctly for easy reference. It organizes results from experiments or studies, allowing researchers and readers to analyze and compare findings quickly. Tables often include titles, headings, and units to ensure clarity of the presented information and facilitate data interpretation during discussions and conclusions.
2) Activity:
Activity refers to the observable outcomes or effects resulting from a particular action or process in scientific studies. In microbiology, for instance, it may denote the effectiveness of an antibacterial agent against bacteria. The level of activity can indicate potency and provide insights into practical applications in treatment or prevention measures.
3) Antibiotic (Antibacterial):
Anti-bacterial describes substances that combat bacteria, including antibiotics. In the face of increasing bacterial resistance, exploring new anti-bacterial agents from natural sources has gained popularity. Investigating the anti-bacterial properties of extracts from plants and fungi contributes significantly to discovering effective treatments for infections caused by resistant bacterial strains.
4) Aureus:
Aureus, specifically referring to Staphylococcus aureus, is a Gram-positive bacterium that can cause various infections in humans, ranging from minor skin infections to severe systemic illnesses like pneumonia. Its ability to form biofilms contributes to its virulence and antibiotic resistance, making it a significant target for research in infectious disease treatments.
5) Study (Studying):
A study is a systematic investigation aimed at discovering or verifying information. In scientific research, it involves hypothesis formulation, data collection, and analysis to draw conclusions. The study outlined explores the antibacterial effects and biofilm inhibition capabilities of a fungal extract against methicillin-resistant Staphylococcus aureus (MRSA), contributing valuable insights.
6) Arrow:
In scientific illustrations and figures, arrows commonly denote directionality or highlight significant features of interest. They guide the reader's attention to specific parts of an image or diagram, aiding in the comprehension of complex data. For example, arrows in microscopy images indicate structural features or changes in treated versus untreated cells.
7) Drug:
A drug is a chemical substance used to treat, cure, prevent, or diagnose diseases. In the context of microbiology, drugs can include antibiotics or other pharmacological agents. Research into new drugs, particularly those derived from natural sources like fungi, is vital in combating emerging and resistant bacterial strains effectively.
8) Surface:
The surface in microbiological contexts refers to any interface where microbial cells can adhere and form biofilms. Understanding surface interactions is critical as biofilm formation on surfaces (natural or artificial) leads to increased resistance against antibiotics. Research focuses on how surfaces can be modified to reduce bacterial adhesion to mitigate infections.
9) Observation:
Observation refers to the act of monitoring and recording characteristics of subjects during experiments. It is fundamental in scientific research, enabling researchers to gather qualitative and quantitative data. Observations of bacterial behavior under different treatments inform conclusions about efficacy and mechanisms of action, shaping future experimental designs and therapeutic strategies.
10) Ocimum sanctum:
Ocimum sanctum, also known as holy basil or tulsi, is a revered medicinal plant in traditional medicine. Extracts from this plant have demonstrated various health benefits and possess antibacterial properties. The study examines endophytic fungi associated with O. sanctum, highlighting its potential as a source for novel antibacterial agents against resistant strains like MRSA.
11) Wall:
The term wall generally refers to the cell wall in bacteria, a rigid structure that provides shape and protection. In the context of MRSA research, disruption of the cell wall is a crucial target for antibacterial strategies. Understanding cell wall integrity is essential for developing effective treatments against multi-drug-resistant strains.
12) Transmission:
Transmission in microbiological contexts often pertains to the methods used to observe microbial cells, such as transmission electron microscopy (TEM). TEM provides high-resolution images, allowing detailed analysis of bacterial structures and the effects of treatments. It enables researchers to gain insights into cellular damage, antibiotic action, and biofilm formation processes.
13) Science (Scientific):
Science is a systematic enterprise that builds and organizes knowledge through observation, experimentation, and analysis. It seeks to explain natural phenomena, develop theories, and inform practices. Within the presented study, science plays a crucial role in understanding bacterial behavior, developing new antimicrobial agents, and addressing the challenges posed by antibiotic resistance.
14) Nature:
Nature in scientific contexts refers to the physical world and the fundamental biological processes that govern living beings. It encompasses ecosystems, organisms, and evolutionary dynamics. Understanding nature assists researchers in discovering new therapeutic agents, such as antifungal extracts, emphasizing the intrinsic connections between living organisms and their environments in health applications.
15) Kumar:
Kumar can refer to a researcher or author contributing to the study. In scientific literature, the recognition of contributors through citations is essential for academic integrity and collaboration. Those engaged in research often draw from previous work by colleagues to build upon foundational knowledge, and Kumar's inclusion indicates active involvement in the broader scientific discourse.
16) Life:
Life refers to the condition distinguishing animals and plants from inorganic matter, characterized by functions such as growth, reproduction, and response to stimuli. In healthcare, understanding life at the cellular level is crucial as it informs the development of therapies targeting specific organisms, such as MRSA, ultimately improving health outcomes and quality of life.
17) Performance:
Performance reflects the effectiveness and efficiency of a treatment or intervention in achieving targeted outcomes. In microbiological studies, performance evaluations include quantifying anti-bacterial activity, biofilm inhibition, or cellular damage. Assessing performance is crucial for determining the viability of new therapeutic agents and guiding the clinical application's practicality and innovation.
18) Education:
Education in scientific fields is vital for advancing knowledge and training researchers to explore and address complex biological challenges. It encompasses sharing findings, methodologies, and principles through academic institutions. Enhancing education improves understanding of emerging issues such as antibiotic resistance, empowering future scientists to develop innovative solutions to pressing health concerns.
19) Transformation (Transform, Transforming):
Transform denotes significant changes in structure, function, or effectiveness. In scientific research, transformations can occur in bacteria during treatment, influencing their behavior and resistance. Understanding how treatments can transform bacterial cells informs the development of effective strategies to combat infections, particularly for resilient strains that pose challenges in conventional therapies.
20) Mutation:
Mutation refers to alterations in the genetic material of organisms, which can lead to changes in traits and potentially impact survival. In the context of bacteria, mutations contribute to antibiotic resistance, complicating treatment efforts. Researching mutations helps in understanding resistant mechanisms and developing targeted strategies to mitigate their effects on public health.
21) Killing (Killed):
Killed refers to the state of bacterial cells that have been rendered non-viable by treatment. In microbiological research, measuring the number of killed cells helps assess the efficacy of antimicrobial agents. Studies showcasing the number of killed bacteria contribute to understanding the effectiveness of new treatments, informing clinical applications and public health strategies.
22) Nahar:
Nahar could refer to a researcher or author involved in the study. Proper attribution is critical in scientific communication, celebrating the contributions of individuals in advancing knowledge. The inclusion of Nahar's name signifies collaboration and collective efforts among researchers to further explore the potential of natural products in combating resistant bacterial strains.
23) Rules:
Rules in a scientific context often denote established protocols or guidelines that govern research methodologies and data interpretation. Following rules ensures rigor and reproducibility in studies, enabling findings to be verified and trusted. Proper adherence to scientific rules ultimately advances knowledge and drives innovation in combating issues such as antibiotic resistance.
24) Death:
Death is the cessation of biological functions that sustain a living organism, a crucial focus in medical research. In the context of bacterial infections, inducing death in pathogenic organisms through effective antimicrobial therapies is the primary goal. Understanding mechanisms that cause or prevent bacterial death informs the development of innovative treatments for resistant strains.
25) Jeya:
Jeya could denote a contributor to the research, emphasizing collaboration within scientific disciplines. The recognition of authors like Jeya in studies highlights the importance of multidisciplinary approaches in investigating biological systems and discovering novel therapeutic solutions. Collaborative efforts often enhance research efficacy and broaden the impact of scientific findings.
26) Ceri:
Ceri may refer to a researcher or co-author mentioned in the study, underscoring the collaborative nature of scientific research. Proper citation of individuals like Ceri signifies their contributions to advancing knowledge, focusing on shared insights into areas such as resistance mechanisms, antimicrobial efficacy, and innovative therapeutic approaches that address pressing global health challenges.
27) Accumulation (Accumulating, Accumulate):
Accumulation refers to the gathering or buildup of substances, including cellular components. In microbiology, accumulation of biofilms or resistance mechanisms can hinder treatment effectiveness. Understanding accumulation processes is crucial in developing strategies to disrupt such formations, providing insights into enhancing antimicrobial efficacy and addressing challenges posed by resistant bacteria in clinical settings.
28) Discussion:
Discussion in scientific writing is a critical section where researchers interpret results, evaluate implications, and propose future research directions. It synthesizes findings, comparing them to existing literature to provide context. The discussion enhances the understanding of experiments, highlights their significance, and identifies pathways for advancing knowledge, particularly concerning antibiotic resistance.
29) Substance:
Substance refers to a specific material or compound, often the focus of scientific studies. In the context of medical research, substances can be natural products or synthetic compounds investigated for their biological effects. Understanding the properties and actions of these substances is crucial for developing new treatments, particularly in combating infections caused by resistant bacteria.
30) Fixation:
Fixation in microbiological contexts involves preserving the morphology and structure of cells in preparation for analysis. It is a crucial step in microscopy, allowing cellular features to be examined under various imaging techniques. Proper fixation ensures accuracy in visualizing and studying the effects of treatments on bacterial cells, including those in biofilms.
31) Quality:
Quality refers to the standard or grade of a particular aspect, such as research methods, experimental data, or results. In scientific studies, ensuring high quality is critical for reproducibility and reliability of findings. Quality control is essential for rigorous experimentation, particularly in assessing new drug efficacy and safety in applications against drug-resistant bacteria.
32) Disease:
Disease denotes a pathological condition affecting an organism, often characterized by specific symptoms or functional abnormalities. In the context of bacterial infections, diseases can range from mild to life-threatening. Understanding the causal bacteria and their interactions is crucial for developing effective treatments, particularly for infections caused by resistant strains like MRSA.
33) Species:
Species in biological classification denotes a group of similar organisms capable of interbreeding. In microbiology, understanding the species of bacteria is critical for determining pathogenic potential and treatment strategies. Research focuses on various bacterial species, particularly drug-resistant strains, to inform clinical approaches and public health interventions effectively.
34) Misuse:
Misuse refers to incorrect or inappropriate use of substances, which can lead to adverse consequences. In the medical field, antibiotic misuse has contributed to the rise of resistant bacteria. Understanding misuse patterns is essential for implementing educational campaigns and guidelines to promote responsible antibiotic use and mitigate the public health crisis of drug resistance.
35) Medium:
Medium denotes the nutrient-rich environment that supports microbial growth in laboratory settings. It can vary widely depending on the experimental aims and microorganisms studied. Understanding how different media affect bacterial species growth is crucial for developing effective antimicrobial treatments and analyzing drug efficacy in studies targeting pathogenic organisms like MRSA.
36) Genu:
Genu refers to a genus in the biological classification system that groups closely related species sharing common characteristics. Classifying bacteria into genera helps researchers understand evolutionary relationships and target specific pathogens more effectively. This taxonomical framework is essential for investigating treatment strategies against resistant bacterial strains, enhancing the understanding of their behavior and virulence.
37) Gold (Golden):
Gold can refer to the gold coating applied to specimens during electron microscopy to enhance image clarity and resolution. This technique ensures better visualization of bacterial structures and biofilms, aiding researchers in assessing the effectiveness of antimicrobial agents. Such enhancements in imaging contribute to the understanding of how treatments impact bacterial integrity and function.
38) Salt (Salty):
Salt often refers to various ionic compounds critical for biological functions, including homeostasis in microbial cells. In microbiological studies, salt concentrations can influence bacterial growth and biofilm formation. Understanding the role of salt in microbial physiology informs treatment strategies, particularly in developing resistance mechanisms in bacteria facing osmotic stress.
39) Post:
Post typically refers to the act of publishing or discussing findings in a scientific context. Posting research findings facilitates information dissemination and collaboration within the scientific community. This exchange of knowledge is essential for advancing research, improving methodologies, and addressing pressing global health concerns, such as antibiotic-resistant infections or emerging pathogens.
40) Pur:
Poor indicates a lack of quality or effectiveness. In scientific studies, results that reflect poor anti-biofilm activity or antibacterial properties raise concerns about the efficacy of tested substances. Recognizing poor outcomes prompts further investigation into treatment strategies and informs improvements in the design of future experiments aimed at overcoming resistance challenges.