Hypolipidaemic and Insulin Secretagogue Activities of (R)-(−)-Carvone

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Journal name: The Malaysian Journal of Medical Sciences
Original article title: Hypolipidaemic and Insulin Secretagogue Activities of (R)-(−)-Carvone
The Malaysian Journal of Medical Sciences (MJMS) is a peer-reviewed, open-access journal published online at least six times a year. It covers all aspects of medical sciences and prioritizes high-quality research.
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Original source:

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Author:

Manal Ahmad Abbas, Ghaleb Ali Oriquat, Manal Mohamed Abbas, Belal Omar Al-Najjar, Yasser Ibrahim Kandil


The Malaysian Journal of Medical Sciences:

(A peer-reviewed, open-access journal)

Full text available for: Hypolipidaemic and Insulin Secretagogue Activities of (R)-(−)-Carvone

Year: 2020 | Doi: 10.21315/mjms2020.27.6.5

Copyright (license): CC BY 4.0


Download the PDF file of the original publication


Summary of article contents:

Introduction

Dyslipidaemias frequently occur in patients with diabetes mellitus, with a notable prevalence of type 2 diabetes among individuals with hyperlipidaemia. This study evaluates the hypolipidaemic effects of (R)-(−)-carvone, a compound found in mint leaves, which potentially lowers both blood glucose and lipid levels. Unlike conventional antihyperlipidaemic agents that often lead to hypoglycaemia, this research aims to determine whether (R)-(−)-carvone can provide a dual benefit in managing lipid and glucose levels without adverse effects on blood sugar.

The Hypolipidaemic Effects of (R)-(−)-Carvone

The study conducted a series of experiments involving a tyloxapol-induced hyperlipidaemia mouse model to assess the efficacy of (R)-(−)-carvone. Results demonstrated that a dose of 100 mg/kg (R)-(−)-carvone significantly reduced plasma triglycerides, total cholesterol, and low-density lipoprotein cholesterol, while not affecting high-density lipoprotein cholesterol. In vitro experiments showed that (R)-(−)-carvone at 500 μM elevated insulin secretion by 454.4% while promoting the proliferation of 1.1E7 human pancreatic β-cells without toxic effects at concentrations up to 100 μM. Furthermore, molecular docking simulations indicated that (R)-(−)-carvone has a good binding affinity to HMG-CoA reductase, suggesting potential mechanisms for its hypolipidaemic effects.

Conclusion

The findings revealed that (R)-(−)-carvone exhibits a hypolipidaemic effect comparable to that of fenofibrate, a standard medication, without inducing hypoglycaemia. Additionally, it significantly enhanced the proliferation and insulin secretion of human pancreatic β-cells in vitro. As a dietary component with FDA approval, (R)-(−)-carvone holds promise for future clinical applications to treat hyperlipidaemia and regulate blood glucose levels in diabetic patients, warranting further investigation into its therapeutic potential.

FAQ section (important questions/answers):

What was the main aim of the study on (R)-(−)-carvone?

The study aimed to find an effective treatment that simultaneously lowers both blood glucose and lipid levels in patients with diabetes mellitus and dyslipidaemias.

What model was used to study the hypolipidaemic effect?

The hypolipidaemic effect of (R)-(−)-carvone was investigated using a tyloxapol-induced hyperlipidaemia mouse model.

What significant results were found with (R)-(−)-carvone treatment?

(R)-(−)-carvone significantly decreased triglyceride and cholesterol levels while increasing insulin secretion and human pancreatic β-cell proliferation.

How does (R)-(−)-carvone compare to fenofibrate in this study?

The hypolipidaemic effects of (R)-(−)-carvone were comparable to fenofibrate, but it did not induce hypoglycaemia in treated animals.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Hypolipidaemic and Insulin Secretagogue Activities of (R)-(−)-Carvone”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Activity:
The term 'Activity' refers to the functional capability of a substance, in this case, (R)-(−)-carvone, to elicit biological effects, such as hypolipidaemic properties. Understanding the activity is essential for evaluating a compound's potential therapeutic applications and mechanisms in treating conditions like hyperlipidaemia and diabetes mellitus.

2) Table:
A 'Table' acts as a structured format for presenting data in research articles. In scientific findings, tables summarize experimental results, such as plasma lipid profiles or differences in treatment effects across groups. This clarity aids in comprehending complex data, allowing for better analysis and interpretation in discussions.

3) Study (Studying):
'Studying' involves systematic investigation to gain deeper insights into a subject. In the context of (R)-(−)-carvone, studying its effects on glucose and lipid metabolism is crucial for advancing our understanding of natural therapies that can benefit metabolic health.

4) Line:
'Line' relates to a lineage or cell line, such as the 1.1E7 human pancreatic β-cell line referenced in research. Cell lines are crucial for experimental biology, helping investigators explore cell behavior, drug effects, and underlying mechanisms without the ethical dilemmas of using primary human tissues.

5) Animal:
'Animal' refers to non-human subjects used in scientific research to model diseases and test new treatments. Animal models, like hyperlipidaemic mice, provide insights into the efficacy and safety of compounds like (R)-(−)-carvone before potential human applications, bridging the gap between laboratory work and clinical trials.

6) Medicine:
'Medicine' encompasses the science and practice of diagnosing, treating, and preventing diseases. Innovations in medicine, enhanced by studies on natural compounds like (R)-(−)-carvone, strive to develop effective therapies for conditions like diabetes and dyslipidaemia, improving overall health outcomes.

7) Disease:
'Disease' signifies abnormal body functions affecting health and well-being, often studied to understand underlying mechanisms and treatments. Research on diseases like diabetes informs the development of targeted therapies and preventive strategies, potentially involving compounds like (R)-(−)-carvone to regulate blood sugar and lipid levels.

8) Drug:
'Drug' refers to a chemical substance used for medical treatment, influencing biological functions. Identifying promising natural drugs, as seen with (R)-(−)-carvone, can lead to new therapeutic options for managing conditions like hyperlipidaemia and diabetes, enhancing patient quality of life.

9) Arrow:
'Arrow' might denote an indicator or direction in scientific diagrams, illustrating relationships or pathways in research findings. Arrows can visually represent the impact of treatments on biological processes, facilitating understanding of complex interactions within the body during experimental studies.

10) Blood:
'Blood' is essential for transporting nutrients, oxygen, and waste within the body. In medical research like the one evaluating (R)-(−)-carvone, blood parameters such as glucose and lipid levels are crucial indicators of metabolic health, necessitating measurement to assess treatment efficacy.

11) Measurement:
'Measurement' indicates the quantification of specific parameters in experiments, ensuring accuracy and reproducibility in science. Accurate measurements, such as plasma lipid profiles, are vital for validating the effects of (R)-(−)-carvone on health outcomes, contributing to credible scientific knowledge.

12) Beta:
'Beta' often refers to β-cells in the context of insulin secretion, highlighting their role in glucose regulation. Investigating how (R)-(−)-carvone affects β-cell function is significant for understanding potential therapeutic approaches in managing diabetes and promoting pancreatic health.

13) Food:
'Food' relates to nutrition and dietary components influencing health. The study of (R)-(−)-carvone highlights the role of dietary elements in managing conditions like hyperlipidaemia, emphasizing how food and natural compounds can contribute to better health outcomes and metabolic balance.

14) Accumulation (Accumulating, Accumulate):
'Accumulation' refers to the buildup of substances, such as lipids in tissues. Understanding lipid accumulation is crucial in research on conditions like hyperlipidaemia and diabetes, as it can inform treatment strategies to reduce excess fat storage and mitigate associated health risks.

15) Species:
'Species' indicates different biological classifications in studies, such as mice or humans. Research on compounds like (R)-(−)-carvone requires understanding both interspecies and intraspecies variations to evaluate treatment efficacy and potential complications across different subject types.

16) Diet:
'Diet' refers to the food and nutrient intake, which significantly impacts health. Studies on (R)-(−)-carvone often consider dietary influences on metabolic diseases, underscoring the importance of nutrition in managing blood sugar levels and lipid profiles in patients.

17) Science (Scientific):
'Scientific' denotes adherence to systematic methodologies in researching and validating hypotheses. The scientific approach ensures that studies on (R)-(−)-carvone are rigorously evaluated, resulting in credible, reproducible findings that can substantiate its application in medical contexts.

18) Girija (Giri-ja):
'Girija' likely refers to the author of a referenced study. Author citations highlight contributions to research in medicine and pharmacology, emphasizing the collaborative nature of scientific discovery in understanding and treating health issues like hyperlipidaemia and diabetes.

19) Danta (Damta):
'Danta' appears to be another author or contributor to research referenced within the study. Acknowledging authorship fosters recognition of individual efforts in collective research endeavors, vital for advancing knowledge and validating findings in medical and pharmacological sciences.

20) Patel:
'Patel' likely refers to one of the authors contributing to the research findings discussed in relation to (R)-(−)-carvone. Acknowledging researchers is fundamental in academia, as their work builds the foundation for scientific progress and encourages collaboration in medicine and health sciences.

21) Nayak:
'Nayak' may denote a contributing author within the study referencing (R)-(−)-carvone. Recognition of various authors is essential in scientific publications, reflecting the collaborative effort involved in advancing research and knowledge that leads to potential applications in healthcare.

22) Water:
'Water' is fundamental for life, and in scientific studies, it often serves as a solvent or medium for conducting experiments. The purity and quality of the water used in research impact the integrity of findings, ensuring accurate results when measuring substances like blood compounds.

23) Cina:
'China' might refer to a geographical context or location of related studies, emphasizing the global significance of research in medical science. Understanding cultural and regional differences in disease prevalence can aid in developing tailored treatment approaches highlighting the importance of international collaboration.

24) Raja:
'Raja' possibly refers to an author involved in a study relevant to (R)-(−)-carvone. Attribution to individual researchers emphasizes the scientific community's collaborative nature and the collective efforts necessary to advance knowledge and develop effective treatments for health conditions.

25) Post:
'Post' may relate to the follow-up or subsequent analysis in a study. Post-hoc analyses help validate study findings and explore additional implications, crucial for drawing comprehensive conclusions about the effects of compounds like (R)-(−)-carvone on health.

26) Discussion:
'Discussion' refers to the section in research papers where findings are interpreted and contextualized. Here, researchers compare results with existing literature, evaluate implications, and propose future directions, making the discussion integral to advancing understanding of compounds and their potential benefits in medicine.

27) Knowledge:
'Knowledge' signifies the accumulated understanding derived from research and studies. Enhancing knowledge about compounds like (R)-(−)-carvone contributes to improved treatment strategies for metabolic disorders, driving scientific innovation, and providing a framework for clinical application in patient care.

28) Fragrance:
'Fragrance' relates to the pleasant scent produced by natural compounds like (R)-(−)-carvone, which is used in various products. Understanding the aromatic properties of such compounds can enrich studies in the field of perfumery while exploring health applications in aromatherapy.

29) Toxicity:
'Toxicity' indicates the degree to which a substance can harm living organisms. Evaluating the toxicity of compounds like (R)-(−)-carvone is essential to ensure safety in therapeutic applications, particularly before conducting clinical trials involving human subjects.

30) Channel:
'Channel' can refer to biological channels, such as ion channels that facilitate cell signaling. Understanding how (R)-(−)-carvone interacts with physiological channels is vital for elucidating its cellular effects, offering insight into its mechanisms of action in diabetes and metabolic health.

31) Company:
'Company' could refer to a pharmaceutical or research organization developing natural therapies like (R)-(−)-carvone. Collaboration with companies enhances the potential for translating research insights into practical treatments, bridging the gap between basic science and clinical application.

32) Potter:
'Potter' may reference a figure or method in research, possibly relating to the apparatus used in experiments. Recognizing tools and methodologies in studies ensures transparency and reproducibility in the scientific process, foundational for validating research findings.

33) Reason:
'Reason' denotes the rationale behind research inquiries. Uncovering the reasons for interest in compounds like (R)-(−)-carvone supports the design of studies aimed at exploring their therapeutic potential, driving innovation in treatment approaches for metabolic diseases.

34) Medium:
'Medium' refers to the environment in which biological experiments occur, typically involving solutions that support cell viability. The choice of media influences experimental outcomes, such as cellular responses to (R)-(−)-carvone, making medium selection pivotal in research protocols.

35) Filling (Filled):
'Filled' could relate to the cellular state or structures within biological systems, such as lipid-filled cells in studies of (R)-(−)-carvone. Understanding cellular morphology assists in assessing treatment effects and potential outcomes in metabolic disorders.

36) Sugar:
'Sugar' indicates glucose or other carbohydrates affecting blood levels. Research on (R)-(−)-carvone often assesses its role in regulating blood sugar levels, crucial for developing effective medications for diabetes and other metabolic disorders.

37) Rules:
'Rules' outline protocols and guidelines governing scientific research. Compliance with established rules ensures the ethical conduct of studies involving natural compounds like (R)-(−)-carvone, promoting responsible investigation within the regulatory frameworks of medical research.

38) House:
'House' may evoke notions of the environment or laboratories associated with research. The design and conditions of research houses significantly affect experimental protocols and outcomes, influencing how compounds like (R)-(−)-carvone are studied within controlled settings.

39) Sign:
'Sign' can imply indicators or markings in research data. Identifying signs of efficacy, toxicity, or clinical relevance in studies on (R)-(−)-carvone helps in assessing its potential therapeutic benefits and guiding treatment decisions.

40) Pose:
'Pose' may refer to the position or arrangement in molecular structures during studies. Understanding the pose of compounds like (R)-(−)-carvone during docking simulations can elucidate molecular interactions and binding affinities, informing their biological effects.

41) Rich (Rch):
'Rich' could describe a condition of abundance, such as rich sources of natural compounds. Evaluating rich environments can help in sourcing (R)-(−)-carvone from plants, contributing to sustainable practices in the development of therapeutic agents.

42) Hand:
'Hand' could refer to the action or manipulation in experiments. Researchers' hands-on involvement in studying (R)-(−)-carvone is essential for accurate data collection and ensuring that experimental conditions are meticulously controlled.

43) Male:
'Male' indicates the gender of animal subjects used in research. The choice between male and female subjects can significantly affect study outcomes, particularly in metabolic research involving compounds like (R)-(−)-carvone, necessitating consideration of gender-specific responses.

Other Health Sciences Concepts:

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Discover the significance of concepts within the article: ‘Hypolipidaemic and Insulin Secretagogue Activities of (R)-(−)-Carvone’. Further sources in the context of Health Sciences might help you critically compare this page with similair documents:

Mentha spicata, Caraway seed, Essential oil, Insulin, Diabetes mellitus, Statistical analysis, Clinical trial, Fasting blood sugar, Ethical committee, Hypoglycaemia, Oxidative stress, Hyperlipidaemia, LDL-C, HDL-C, Total cholesterol, Low-density lipoprotein cholesterol, High-density lipoprotein cholesterol, Type 2 Diabetes Mellitus, FBS, Epidemiologic studies, Type 2 diabetes, Antimicrobial agent, Histological examination, Molecular docking, Binding affinity, Binding site, Insulin Dependent Diabetes Mellitus, Histopathological studies, In vitro studies, Cell viability, Positive control group, PubMed, Google Scholar, Lipid metabolism, Atherogenic Index, Insulin secretion, Inflammatory cells, Glucose metabolism, Confidence interval, Normal saline, One-way analysis of variance, Blood glucose level, Antihyperlipidemic activity, Analysis of variance, Blood glucose, Ethical approval, In vivo studies, Catalase activity, Insulin release, Hypercholesterolemia, Cytotoxic effect, Lipid accumulation, Degenerative change, Fat accumulation, Liver tissue, Cardiovascular risk, Hepatic enzymes, Mouse model, Negative control group, Binding energy, Hypolipidaemic effect, Fenofibrate, Oxidative stress parameters, HMG-CoA reductase, Cholesterol biosynthesis, Hydrogen bond, Hydrogen bond interaction, Hydrophobic interaction, Lamarckian genetic algorithm, Side effect, Rotatable Bonds, Fluorescent microscope, Biochemical parameter, FDA, Total protein assay, Natural compound, Root mean square deviation, Docking simulation, Cell line, Free energy of binding, Triton WR-1339, Blood cholesterol, Molecular Docking Simulation, Immunofluorescence assay, Retro-orbital plexus, GSH LEVEL, RMSD, Hypocholesterolaemic effect, Pellet Diet, Eosinophilic cytoplasm, Shapiro–Wilk test, Dyslipidaemias in diabetes, Hyperlipidaemic patients, R-carvone binding affinity, Tyloxapol-induced hyperlipidaemia, Fenofibrate comparison, Glucose-stimulated insulin secretion, Hypoglycaemia avoidance, Oxidative stress impact, Herbal remedies effectiveness, Lipid level, RPMI-1640, Apoptotic cell, Plasma cholesterol level, Antioxidant test, Plasma triglyceride, HMG-CoA reductase enzyme, Diabetic rat, Docking parameters, Herbal Remedie, Rie, Fenofibrate-treated group, Chemiluminescent Microparticle Immunoassay, Diabetic rat model, Animal use, Serum lipid, Ionic interaction, LDL-C level, Food Chem. Toxicol., CrossRef, CO2 incubator, TG, Plasma TGs, Proliferation, Carvone, Human pancreatic, Hepatic triglyceride, 1.1E7 cell line, Anti-hyperlipidaemic drugs, Statins and fibrates, Mint leaves, Rodent cells, Intraperitoneal treatments, Fenofibrate dose, Tyloxapol dose, Liver specimens, Total GSH level, AutoDockTools, MTT kit, Krebs-Ringer bicarbonate buffer, BrdU, Zeiss fluorescent microscope, Hepatic TGs, AbstractBackgroundDyslipidaemias, Proliferation of 1.1E7, Hepatic triglyceride level, Tyloxapol, Biosynthesis of cholesterol, Clearance of triglycerides, Fragrances and flavours, TG content, 2R4F crystal structure, Co-crystallised inhibitor, GraphPad Prism, Tukey-Kramer post analysis, Hepatic total GSH, Fat droplets, Plasma TC, S-carvone, Hepatic TG content, HMG-CoA, Flavor ingredients, Inflammatory cell, Hyperlipidaemia mice model, Hypoglycaemia in animals, Caraway odour, Mint odour, Human islets of Langerhans, PDB crystal structures, Grid box size, Insulin release studies, Chemiluminescent microparticle immunoassay technology, Shapiro-Wilk test, Plasma lipids profile, Tyloxapol injection, Blood glucose and lipid levels, Insulin secretion and proliferation, Multifactorial intervention strategies, Liver TG content, 1.1E7 Viability, Hypolipidaemic drugs, Hyperlipidaemic drugs, Tyloxapol administration, Carvone compounds, Food Chem Toxicol.

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