Zinc and Ischemia Pre-conditioning in Renal Injury
Journal name: The Malaysian Journal of Medical Sciences
Original article title: Zinc Supplementation and Ischemia Pre-conditioning in Renal Ischemia/Reperfusion Injury
The Malaysian Journal of Medical Sciences (MJMS) is a peer-reviewed, open-access journal published online at least six times a year. It covers all aspects of medical sciences and prioritizes high-quality research.
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Original source:
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Bahar Mazaheri, Fatemeh Emami, Fatemeh Moslemi, Ardeshir Talebi, Mehdi Nematbakhsh
The Malaysian Journal of Medical Sciences:
(A peer-reviewed, open-access journal)
Full text available for: Zinc Supplementation and Ischemia Pre-conditioning in Renal Ischemia/Reperfusion Injury
Year: 2019 | Doi: 10.21315/mjms2019.26.4.5
Copyright (license): CC BY 4.0
Download the PDF file of the original publication
Summary of article contents:
Introduction
Renal ischemia/reperfusion (RIR) injury represents a critical clinical challenge, often leading to severe kidney dysfunction during various medical situations such as surgeries, transplants, and resuscitation. This complex phenomenon is associated with the formation of reactive oxygen species (ROS), inflammation, and cellular damage, all contributing to acute kidney injury (AKI). In recent years, strategies like ischemic preconditioning (IPC) and the use of antioxidants such as zinc (Zn) have been explored to mitigate these harmful effects. This study evaluates the protective roles of IPC alongside Zn supplementation in a rat model subjected to RIR.
The Role of Zinc Supplementation in Protecting Against RIR Injury
The findings of the study underscore the significant protective role of Zn supplementation in mitigating the adverse effects caused by RIR. The administration of Zn sulphate prior to inducing RIR notably reduced elevated serum creatinine levels and improved kidney tissue integrity compared to the control group that experienced RIR without treatment. Conversely, while IPC was applied to enhance kidney resilience through brief ischemic challenges, it alone, or in combination with Zn, did not improve the biochemical and histological markers affected by RIR. This suggests that the protective mechanisms offered by Zn may be more effective than IPC under the conditions tested, highlighting the need for further investigation into optimal protocols for IPC durations and their interactions with other treatment modalities.
Conclusion
This research concludes that Zn supplementation delivers significant protection against renal ischemia/reperfusion injury in rats, whereas IPC alone or in combination with Zn fails to provide similar protective effects. This disparity underscores the potential limitations of IPC as a strategy in certain contexts of RIR, suggesting that more extensive ischemic periods may be necessary to leverage its benefits fully. The findings call for further studies to clarify the underlying mechanisms at play, particularly regarding the interactions of various treatment strategies in combating kidney injury. Overall, strategies focusing on Zn supplementation may offer a more robust approach to mitigating RIR damage in clinical settings.
FAQ section (important questions/answers):
What is the purpose of the study on renal ischemia/reperfusion?
The study aimed to investigate the effects of pre-conditioning ischemia (IPC) and zinc (Zn) supplementation on renal ischemia/reperfusion (RIR) injury in rats, focusing on their protective roles against kidney dysfunction.
What methods were used in this renal injury study?
The study involved 63 Wistar rats divided into five groups, subjected to either sham operation, renal ischemia/reperfusion, zinc supplementation, IPC, or a combination of treatments. Various biochemical and histological measurements were performed to evaluate kidney damage.
What were the main findings regarding zinc supplementation?
Zinc supplementation significantly reduced serum creatinine levels and improved kidney injury compared to the ischemic group. However, no protective effects were observed with IPC alone or when combined with zinc.
What conclusions were drawn about IPC in this study?
The study concluded that IPC alone did not protect against renal ischemia/reperfusion injury. In contrast, single-dose zinc administration showed protection, but co-treatment with IPC did not enhance outcomes.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Zinc and Ischemia Pre-conditioning in Renal Injury”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Table:
A table is a method of organizing data systematically in rows and columns, used extensively in scientific literature to present complex information clearly. In this context, tables compare results between experimental groups, showing numerical relationships, such as biochemical measurements, helping readers quickly grasp key findings of the study.
2) Study (Studying):
A study refers to a systematic investigation of a specific topic or phenomenon to discover new insights or validate existing knowledge. In scientific research papers, studies often outline methods, results, and conclusions, providing a structured format to share findings with the broader scientific community and contribute to knowledge advancement.
3) Male:
In this context, 'male' specifies the sex of experimental animals used in the study, emphasizing potential gender differences in response to interventions like zinc supplementation during renal ischemia/reperfusion injury. These distinctions are crucial, as male and female physiology can affect drug metabolism and the efficacy of therapeutic strategies.
4) Animal:
Animal refers to the non-human subjects used in scientific research to study biological processes, disease mechanisms, and therapeutic interventions. Studies on animals are essential for understanding human health, allowing researchers to explore effects in controlled environments, thereby generating foundational knowledge for future clinical applications in medicine.
5) Post:
The term 'post' typically indicates a timeframe following an event or intervention. In medical research, this often refers to assessments or observations made after a treatment or surgical procedure, such as measuring kidney function after ischemia/reperfusion injury to evaluate the effectiveness of therapeutic interventions in restoring function.
6) Inflammation:
Inflammation is a biological response of body tissues to harmful stimuli, such as injury or infection. It plays a central role in renal ischemia/reperfusion injury, contributing to tissue damage and dysfunction. Understanding this process is vital for developing therapeutic strategies aimed at mitigating or preventing kidney damage in clinical settings.
7) Rules:
Rules refer to the principles or guidelines that govern experiments and studies to ensure integrity and reproducibility. In the context of scientific research, following established rules ensures proper ethical treatment of subjects, accurate data collection, and valid interpretations of results, reinforcing the study's credibility amongst the scientific community.
8) Blood:
Blood is a vital fluid in living organisms responsible for transporting oxygen, nutrients, and waste products. In the context of renal studies, blood parameters often indicate kidney function and health. Analyzing blood samples can reveal the impact of interventions, such as medications or surgical methods, on overall kidney health.
9) Measurement:
Measurement in scientific research refers to the process of quantifying variables to draw conclusions about phenomena. Accurate measurements are crucial for validating hypotheses, comparing experimental results, and ensuring the reliability of findings. In this study, measurements relate to biomarkers of kidney injury and recovery from ischemia/reperfusion.
10) Activity:
Activity can refer to various forms of physiological or biochemical processes of interest in research. In this study, activity may refer to enzymatic functions or metabolic responses, which indicate how well the kidney is operating and can provide evidence for the efficacy of treatments administered during the experiments.
11) Science (Scientific):
Science encompasses the systematic study of the natural world through observation, experimentation, and analysis. In research contexts, it provides the framework for understanding mechanisms of diseases, developing treatments, and validating findings through peer review and reproducibility, vital for advancing knowledge and improving healthcare outcomes.
12) Sam (Sham):
The term 'sham' refers to a control group in experiments where subjects undergo procedures without the active treatment being investigated. This allows researchers to compare outcomes meaningfully against real intervention groups, ensuring that any observed effects are attributable to the treatment rather than simply the procedure itself.
13) Ter:
Ther relates to therapeutic approaches or interventions, often discussed in the context of treating diseases or injuries. This term suggests a focus on methods used to alleviate or cure conditions, essential in clinical research for evaluating the effectiveness of treatments, such as pharmacological agents or surgical techniques.
14) Pharmacological:
Pharmacological pertains to the study or application of drugs and their effects on biological systems. In research, pharmacological interventions are rigorously tested to understand their potential benefits and limitations in treating diseases, including side effects, mechanisms of action, and overall efficacy in enhancing health outcomes.
15) Discussion:
Discussion in scientific papers is where researchers interpret and contextualize their findings, comparing them with existing literature. This section presents potential implications of the results, explores limitations, and suggests directions for future research, helping to bridge empirical data with broader scientific understanding and clinical applications.
16) Toxicity:
Toxicity refers to the degree to which a substance can harm living organisms. In biomedical research, understanding the toxicity of substances, including drugs and interventions, is crucial for ensuring safety in treatments, particularly when evaluating new therapeutics or assessing the side effects of established ones in clinical settings.
17) Relative:
Relative indicates a comparative perspective, often used to assess how one factor relates to another, such as comparing kidney function before and after treatment. In studies, relative changes in measurements can help identify significant effects or responses, providing a clearer understanding of the treatment's impact.
18) Incision:
Incision refers to a surgical cut made during a medical procedure, essential for accessing internal structures. In studies involving models of surgery or injury, the creation of incisions can be critical for simulating conditions like ischemia/reperfusion injury in research, allowing for controlled investigations of healing and treatments.
19) Disease:
Disease refers to a pathological condition characterized by specific symptoms and signs resulting from abnormalities in body structures or functions. In research, understanding diseases is paramount for developing prevention strategies, treatments, and improving patient outcomes, leading to innovative approaches in medical care and therapy.
20) Species:
Species refer to distinct groups of organisms that can interbreed and produce fertile offspring. In biomedical research, understanding species differences is crucial as experimental results in one species may not directly translate to another, necessitating careful consideration in the design of studies involving animal models.
21) Roman (Roma):
Roman in this context may not have a direct connection to scientific content but could imply the use of Roman numerals for listing or categorizing items. Consistent formatting aids in clarity, allowing researchers to present structured data or findings systematically, enhancing comprehension in scientific communication.
22) Chang:
Chang may refer to a specific study, researcher, or methodology in this context. Familiarity with research conducted by particular scientists or teams is essential in academia, providing credibility to claims and emphasizing the evolution of scientific thought, and how it influences present research practices and findings.
23) Cage:
Cage often refers to a controlled environment where laboratory animals are housed during experiments. Properly designed cages are vital for animal welfare, facilitating accurate data collection while minimizing stress or behavior alteration, which may affect study results. Housing conditions can significantly impact the outcomes of biomedical research.
24) Salt (Salty):
Salt, particularly in research concerning kidney physiology, often pertains to sodium chloride and its effects on fluid balance, blood pressure, and renal function. Understanding salt's role in health and disease is critical in studies evaluating renal response to ischemia/reperfusion and treatments aimed at mitigating injury and preserving function.
25) Drug:
Drug refers to a substance used in medical treatment, affecting biological processes. In research, drugs are studied for therapeutic potential, mechanisms of action, and safety. Exploring their effects on injury models, such as renal ischemia/reperfusion, is essential for improving treatment strategies and clinical outcomes in patient care.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Zinc and Ischemia Pre-conditioning in Renal Injury’. Further sources in the context of Science might help you critically compare this page with similair documents:
Rat, Inflammation, Anti-inflammatory activities, Oxidative stress, Reactive oxygen species, Renal transplantation, Acute Renal Failure, Gene expression, Antioxidant Effect, Renal function, Male Wistar rats, Protective effect, Renal failure, Acute Kidney Injury, Zinc, Inflammatory response, Kidney disease, Ischemic injury, Renal dysfunction, Ischemia, Ischemia-reperfusion injury, Trace element, Female Wistar rats, Free radical scavenger, Wistar rat, Ischemic preconditioning, Ischemia followed by reperfusion, Renal injury, Serum level, Oxygen consumption, Zinc supplementation, Blood sample, Zinc chloride, Biochemical Measurement, Kidney dysfunction, Ischemia-reperfusion, Kidney, Histological Measurement, Kidney Injury, Tissue inflammation, Urine flow rate, Cellular apoptosis, Serum levels of creatinine, Kidney Tissue Damage, Zinc acexamate, Renal tissue.