Electrophoretic Techniques for the Detection of Human Microsatellite D19S884
Journal name: The Malaysian Journal of Medical Sciences
Original article title: Electrophoretic Techniques for the Detection of Human Microsatellite D19S884
The Malaysian Journal of Medical Sciences (MJMS) is a peer-reviewed, open-access journal published online at least six times a year. It covers all aspects of medical sciences and prioritizes high-quality research.
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Fazliana MANSOR, Liyana ZAMRI, Siti Sarah HAMZAH
The Malaysian Journal of Medical Sciences:
(A peer-reviewed, open-access journal)
Full text available for: Electrophoretic Techniques for the Detection of Human Microsatellite D19S884
Year: 2015
Copyright (license): CC BY 4.0
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Summary of article contents:
Introduction
Microsatellites, which are short tandem repeats of nucleotides in the genome, play a crucial role in genetic studies, particularly in the context of complex diseases such as polycystic ovary syndrome (PCOS). PCOS is the most prevalent reproductive endocrine disorder among women of childbearing age and is linked to significant reproductive issues, including anovulatory infertility. This research specifically focuses on the microsatellite marker D19S884, located on chromosome 19p13.2, which has shown associations with PCOS. The study aimed to detect and analyze this microsatellite in DNA samples extracted from the blood of PCOS patients, intending to contribute to the understanding of the genetic underpinnings of this condition.
The Utility of Capillary Electrophoresis for Microsatellite Analysis
One significant concept discussed in the study is the use of capillary electrophoresis for fragment analysis of the D19S884 microsatellite. The traditional method of separating PCR products uses low-melting agarose (LMA) gel electrophoresis, which, while adequate, can make it challenging to distinguish between alleles due to similarities in banding patterns. The researchers employed capillary electrophoresis to enhance the resolution of allele identification. This technique allowed for the detection of even minor differences in allele lengths, demonstrating a capacity for precise allelic discrimination. By comparing results from both gel and capillary methods, the study affirmed that capillary electrophoresis provides a significant advantage in accurately characterizing microsatellite polymorphisms.
Conclusion
The combination of low-melting agarose gel electrophoresis and capillary electrophoresis has proven effective for analyzing the D19S884 polymorphism related to PCOS. The study found that while gel electrophoresis is a simpler preliminary method, capillary electrophoresis offers a more refined analysis necessary for understanding this genetic marker’s role in the disorder. With insights gained from this comprehensive review of methodologies, further investigations into the allele frequencies of D19S884 among Malaysian women with PCOS could help establish genetic predispositions in the region. This research underscores the potential of these techniques to improve the diagnostic capabilities and genetic understanding of complex conditions like PCOS.
FAQ section (important questions/answers):
What is the significance of microsatellite marker D19S884 in PCOS?
Microsatellite marker D19S884 is associated with polycystic ovary syndrome (PCOS), a common reproductive endocrine disorder impacting women's fertility. It serves as a genetic marker to help understand the genetic factors contributing to PCOS.
How are DNA microsatellites detected in this study?
DNA microsatellites are detected using polymerase chain reaction (PCR) amplification with specific fluorescently labeled primers. The PCR products are subsequently analyzed using both gel electrophoresis and capillary electrophoresis for detailed fragment analysis.
What techniques are used for visualizing PCR products?
PCR products are visualized using low-melting agarose gel electrophoresis with GelRed™ staining, which is a safer alternative to ethidium bromide. Capillary electrophoresis is also utilized for high-resolution analysis of the fragment sizes.
What was the main conclusion of this research?
The study established a standardized protocol combining gel electrophoresis and capillary electrophoresis for accurately identifying genetic biomarkers related to PCOS, specifically the D19S884 microsatellite polymorphism.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Electrophoretic Techniques for the Detection of Human Microsatellite D19S884”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Table:
In scientific research, a 'table' refers to a systematic arrangement of data or information, typically presented in rows and columns. Tables help organize complex data for easy interpretation and analysis, facilitating comparisons among different sets of results or observations in a study. They serve as critical tools for summarizing findings in a clear and concise manner.
2) Study (Studying):
'Study' denotes a systematic investigation conducted to gain knowledge or understand a particular phenomenon. In the context of scientific research, it often involves hypothesis formulation, data collection, analysis, and interpretation. Studies can vary in scope and design, contributing to the body of evidence supporting or refuting scientific propositions.
3) Disease:
'Disease' refers to a pathological condition of a tissue, an organ, or the entire body that disrupts normal functioning. Diseases may arise from various factors, including genetics, infections, or environmental influences. Understanding diseases, such as polycystic ovary syndrome (PCOS), is crucial for developing targeted treatments and improving public health outcomes.
4) Blood:
'Blood' is a vital fluid in the body responsible for transporting oxygen, nutrients, hormones, and waste products. It plays a critical role in the immune system and maintaining homeostasis. In genetic studies, blood samples are often used for DNA extraction to analyze genetic markers associated with diseases or conditions.
5) Family:
'Family' indicates a group of individuals related by blood or marriage. In genetics, family structures are essential for pedigree analysis, helping to track inheritance patterns of traits, including genetic diseases. By studying family histories, researchers can identify risk factors and genetic predispositions that contribute to the overall understanding of certain diseases.
6) Rules:
'Rules' denote established guidelines or principles governing behavior and processes in various disciplines. In scientific research, they can refer to methodologies that govern studies, including regulations for sample collection, data analysis, and ethical treatment of subjects. Adhering to rules ensures the validity, reliability, and integrity of scientific findings.
7) Science (Scientific):
'Science' encompasses a systematic enterprise that builds knowledge through observation, experimentation, and analysis of the natural world. It includes various disciplines such as biology, chemistry, and physics. The scientific method is foundational within science, ensuring that findings are grounded in empirical evidence and contributing to our understanding of complex phenomena.
8) Post:
'Post' is a prefix indicating something that occurs after a certain event or time. In academia, 'post' may refer to work conducted after a notable study or the dissemination of results following the completion of research. In publishing, 'post' often indicates information updated or provided after the initial release.
9) Surrounding:
'Surrounding' refers to the area or environment around a specific object or entity. In scientific contexts, it often pertains to external factors that may influence an experiment or outcome. Analyzing surrounding influences, such as environmental conditions or demographic characteristics, can enhance the interpretation of research findings, leading to more comprehensive conclusions.
10) Agriculture:
'Agriculture' is the science and practice of farming, including the cultivation of plants and rearing animals. It plays a crucial role in food production and economy. Research in agriculture often investigates genetic factors that contribute to crop yield or livestock health, utilizing techniques such as DNA analysis to identify desirable traits.
11) Mutation:
'Mutation' signifies a change in the DNA sequence that may lead to altered physical traits or biological functions. In genetics, mutations can drive evolution or contribute to diseases, making their study essential for understanding hereditary conditions and developing therapeutic strategies aimed at correcting these genetic alterations.
12) Painter:
'Painter' in the context of research may refer to individuals involved in specific expressions of art or design. In scientific studies, it may metaphorically apply to disciplines where visual representation is crucial, such as genomics, where graphs or illustrative diagrams visualize genetic relationships or findings across various populations and conditions.
13) Cotton:
'Cotton' refers to a fibrous plant known for its soft fibers, which are harvested and processed for use in textiles. In agricultural genetics, studies may focus on cotton crops to understand genetic diversity, develop disease-resistant varieties, or enhance yield. Research related to cotton cultivates insights into both environmental and economic sustainability.
14) Malik:
'Malik' appears as a specific name in the context of the research. It may denote a researcher or individual involved in a study. The significance of names in scientific communications underscores the collaborative nature of research and the contributions made by various scholars toward advancing knowledge in their fields.
15) Dane (Dané):
'Dane' could refer to individuals from Denmark or a historical context related to the Danish people. In research, specific populations, including Danes, may be studied for genetic traits, health conditions, or other attributes, helping to understand population-specific phenotypes and their implications for broader biological or medical inquiries.
16) Discussion:
'Discussion' is a critical section of scientific papers where authors interpret their findings in the context of existing literature. It offers insights into the implications, limitations, and potential future directions of research. A robust discussion fosters dialogue within the scientific community, paving the way for further investigations and understanding of complex issues.
17) Quality:
'Quality' relates to the standard or degree of excellence of a product, service, or process. In research, quality assurance practices ensure that studies yield valid, reliable, and credible results. High-quality research is fundamental for advancing knowledge, influencing policy decisions, and contributing to the overall integrity of scientific inquiry.
18) India:
'India' is a country in South Asia with a diverse population and rich cultural heritage. In a scientific context, studies may focus on health issues, such as the prevalence of polycystic ovary syndrome (PCOS), to understand genetic predispositions and disease impacts within specific demographics. Data from India contributes to the global understanding of health disparities.
19) Cina:
'China' is a populous country in East Asia known for its significant contributions to science and technology. Research from China often addresses public health issues, environmental concerns, and genetic diversity. Understanding the genetic factors associated with diseases in China, such as PCOS, enhances global health knowledge and informs health interventions.
20) Miṇi (Mini):
'Mini' generally refers to something small or of reduced size. In scientific contexts, 'mini' may apply to equipment, such as miniaturized lab instruments or kits used for DNA extraction and analysis. These innovations are pivotal for enhancing the accessibility and efficiency of laboratory procedures while maintaining scientific rigor.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Electrophoretic Techniques for the Detection of Human Microsatellite D19S884’. Further sources in the context of Science might help you critically compare this page with similair documents:
Polymerase chain reaction, Anovulatory infertility, Prevalence of PCOS, Microsatellite markers, Polycystic Ovary Syndrome, PCR amplification, Capillary electrophoresis, Genomic DNA Extraction, PCR product, Blood sample, Capillary gel electrophoresis, Clinical research ethics.