MiR-3099 is upregulated in differentiating 46c ES cells during neural induction.
Journal name: The Malaysian Journal of Medical Sciences
Original article title: MiR-3099 is Overexpressed in Differentiating 46c Mouse Embryonic Stem Cells upon Neural Induction
The Malaysian Journal of Medical Sciences (MJMS) is a peer-reviewed, open-access journal published online at least six times a year. It covers all aspects of medical sciences and prioritizes high-quality research.
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Shahidee ZAINAL ABIDIN, Maryam ABBASPOURBABAEI, Carolindah Makena NTIMI, Wei-Hong SIEW, CHEAH Pike-See, Rozita ROSLI, Norshariza NORDIN, King-Hwa LING
The Malaysian Journal of Medical Sciences:
(A peer-reviewed, open-access journal)
Full text available for: MiR-3099 is Overexpressed in Differentiating 46c Mouse Embryonic Stem Cells upon Neural Induction
Year: 2014
Copyright (license): CC BY 4.0
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Summary of article contents:
Introduction
MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression and protein synthesis, playing significant roles in various biological processes, including nervous system development. One such miRNA, miR-3099, is highly expressed in the central nervous system of developing mouse embryos. This study aims to investigate the expression of miR-3099 during the differentiation of 46C mouse embryonic stem (mES) cells after neural induction using a specialized medium. The research focuses on understanding how miR-3099 may influence the differentiation of neural progenitor cells into neurons, astrocytes, and oligodendrocytes.
Role of miR-3099 in Neural Differentiation
The findings revealed that miR-3099 expression was consistent throughout the in vitro differentiation process of 46C mES cells. Notably, the expression levels of miR-3099 were found to peak at days 11, 17, and 22 post-neural induction, indicating that this miRNA may play a crucial regulatory role in neural stem cell differentiation. The study also observed a heterogeneous cell population with characteristics of stem cells, neural progenitors, and differentiated neural cells over a 22-day period. These observations suggest that miR-3099 could be pivotal in modulating the differentiation of neural cells within the developing nervous system.
Conclusion
In conclusion, the upregulation of miR-3099 during the differentiation of 46C mES cells highlights its potential importance in neuronal development. While the findings suggest a role for miR-3099 in regulating neural stem cell differentiation, further investigation in more optimized neural stem cell cultures is necessary to pinpoint its precise cellular functions and molecular targets. This research contributes to the understanding of miRNAs in neurogenesis and presents a basis for future studies aimed at elucidating the molecular mechanisms governing neural differentiation and development.
FAQ section (important questions/answers):
What role do microRNAs play in the central nervous system?
MicroRNAs (miRNAs) are crucial for regulating gene expression and protein synthesis, primarily affecting developmental processes, particularly in the central nervous system, by controlling various post-transcriptional processes.
How was miR-3099 expression analyzed during differentiation?
The expression of miR-3099 was analyzed through stem-loop pulse RT-PCR across different time points during the neural differentiation of 46C mouse embryonic stem cells after neural induction.
What findings were observed regarding neuronal differentiation?
After neural induction, the 46C mES cells differentiated into various cell types, including neurons, astrocytes, and oligodendrocytes, demonstrating a heterogeneous pool of neural lineage cells over time.
What conclusions were made about miR-3099 in differentiation?
The study concluded that miR-3099 expression increased significantly during the differentiation process, suggesting it may regulate neural stem cell differentiation and play a vital role in neurogenesis.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “MiR-3099 is upregulated in differentiating 46c ES cells during neural induction.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Table:
In scientific literature, a 'Table' is a method of organizing data and results in a clear format, allowing for easy comparison and analysis. Tables summarize key findings, facilitating readers to quickly grasp experimental conditions, results, or other metrics without parsing long texts. They enrich research presentations and papers significantly.
2) Study (Studying):
A 'Study' refers to a systematic investigation aiming to establish facts or principles. In research like this, it's crucial for generating reliable data and insights. This particular study focuses on the expression of a specific microRNA during neural induction, intending to uncover its role in neural differentiation processes.
3) Developing:
The term 'Developing' is significant in biological research, especially concerning organisms or tissues undergoing growth and differentiation. In this context, it refers to processes happening in mouse embryos or stem cells as they mature and form structured tissues, which is pivotal for understanding developmental biology and related mechanisms.
4) Medium:
In cell culture, 'Medium' refers to the nutrient-rich solution that supports cell growth. The choice of medium, such as N2/B27 in this study, is critical for induction processes, as it provides essential nutrients, growth factors, and conditions necessary for the differentiation of embryonic stem cells into neural progenitors and neurons.
5) Purification:
'Purification' indicates the process of isolating specific cell types from a mixed population. In this research, it is essential for obtaining neural progenitor cells. The purification techniques enhance the study's quality by ensuring that the specific cell types of interest are accurately analyzed for their properties and behavior.
6) Education:
'Education' is broadly about imparting knowledge. While not directly discussed in the study, it underscores the importance of educating researchers and practitioners about differentiation processes and microRNA roles, which can inform advancements in regenerative medicine and therapeutic strategies for neurodegenerative diseases and other health issues.
7) Observation:
'Observation' is a foundational aspect of scientific research, involving collecting data through careful monitoring and study of processes or phenomena. In this study, observations about cell differentiation at various stages enhance the understanding of developmental biology and the specific roles of microRNAs in neural differentiation.
8) Disease:
'Disease' denotes pathological conditions affecting organisms. Understanding cellular differentiation and the role of specific microRNAs, such as miR-3099, can inform disease mechanisms in neurodevelopmental disorders or neurodegenerative diseases. Insights gained from this study may yield new approaches for treatment and management of such conditions.
9) Gelatin:
'Gelatin' is a substance often used in laboratory settings to provide a surface for cell culture. In this example, it serves as a coating material for plates, promoting cell attachment and growth during embryo stem cell culture and differentiation, which is important for studying cellular behaviors.
10) Nature:
In scientific literature, 'Nature' can refer to the natural world and biological phenomena or a prominent scientific journal that publishes research across various fields. Emphasizing the natural processes of cell differentiation and genetic regulation, this study contributes to the body of knowledge regarding neural development.
11) Chada:
'Chada' references Dr. K. Chada, a contributing author in the study. Acknowledging authors provides context to the research's credibility, allowing readers to identify the expertise behind the findings. The contributions from researchers with diverse backgrounds facilitate thorough understanding and advancements in scientific knowledge.
12) Putra:
'Putra' refers to Universiti Putra Malaysia (UPM), the academic institution supporting the research. Affiliations with reputable institutions add credibility to findings and ensure rigorous academic standards. Support from such institutions often enables groundbreaking research in areas like stem cell biology and neurogenesis.
13) Road:
'Road' symbolizes the pathway to knowledge and discovery in scientific research. In the context of this study, it represents the journey of exploring neurogenesis processes, revealing mechanisms underlying neural differentiation, and paving the way for future studies aimed at understanding and addressing neural developmental issues.
14) Line:
'Line' in biological research often refers to lineage or a specific type of cells. In this article, the terms refer to neural lineage, highlighting the differentiation path and identity of cells derived from embryonic stem cells into neural progenitors and neurons, essential for studying neurodevelopment.
15) Post:
'Post' may refer to the publication or dissemination of research findings. Scholars and scientists share their observations and insights with the community through journals or conferences, enhancing overall scientific literacy. This study's findings could impact future research directions in embryonic stem cell biology and neurogenesis.
16) Ter:
'Ther' might refer to therapy or therapeutic implications of research findings. Understanding neural stem cell differentiation and the regulation of associated microRNAs could inform therapeutic strategies in treating neurodegenerative diseases or injuries, emphasizing the practical applications of research within medical sciences.
17) Discussion:
'Discussion' is a vital section in scholarly articles where authors interpret their findings, address implications, strengths, limitations, and suggest future research directions. It contextualizes results within the broader scientific landscape, promotes dialogue, and encourages collaborative advancements, further enhancing understanding in fields such as neuroscience.
18) Donkey:
'Donkey' refers to a specific secondary antibody (donkey anti-mouse IgG) used in immunohistochemistry assays. Such antibodies are crucial for the visualization of specific proteins in cell types. Accurate labeling aids in understanding the differentiation state and development of neural progenitor cells in the study.
19) Mason:
'Mason' likely refers to John Mason, a key contributor to the research, potentially providing insights or initial cell lines used. Mentioning contributors adds academic rigor, promotes collaboration, and enriches the narrative by acknowledging expertise that leads to research advancements.
20) Rules:
'Rules' indicate the scientific principles or methodologies guiding cell culture and experimentation. Strict adherence to established protocols is essential for replicable results and valid conclusions. This study follows recognized practices for neural induction and stem cell analysis, reinforcing the integrity of scientific research.
21) Pulse:
'Pulse' may reference specific methodologies, such as pulse RT-PCR. This technique allows real-time monitoring of gene expression, providing insights into temporal changes in microRNA levels during differentiation. Techniques like pulse RT-PCR enhance the understanding of transcriptional regulation during dynamic biological processes.
22) Life:
'Life' signifies living organisms, encompassing the subjects of biology research. In this study, understanding cellular life in terms of differentiation and development is crucial, as insights into developmental processes enhance knowledge that could lead to medical advancements for living organisms, including humans.
Other Science Concepts:
Discover the significance of concepts within the article: ‘MiR-3099 is upregulated in differentiating 46c ES cells during neural induction.’. Further sources in the context of Science might help you critically compare this page with similair documents:
Nervous system, Central nervous system, Embryonic development, Differentiation process, Gene expression, Astrocytes, RT-PCR, Stem cell niche, Reverse transcriptase-polymerase chain reaction, Cellular function, In vitro model, Protein synthesis, Motor neuron, Adult neurogenesis, Subventricular zone, Neural precursor cells, Immunocytochemistry, Pluripotent Stem Cells, Pluripotent Cells, Stem cell, Cellular differentiation, Stem cell differentiation, Immunocytochemistry (ICC), MicroRNA, Ground state, Molecular target, Epigenetic regulation, Fluorescence-activated cell sorter, Gene expression regulation, Neural differentiation, Nuclear receptor, Neural progenitor cells, Mouse embryonic stem cells, Neuronal differentiation, Mature neurons, Neural stem cell, Neuronal precursor cells, Reporter gene, Embryonic stem cell.