Adipose-Derived Stem Cells for Urinary Bladder Smooth Muscle Regeneration
Journal name: The Malaysian Journal of Medical Sciences
Original article title: Human Adipose Tissue Derived Stem Cells as a Source of Smooth Muscle Cells in the Regeneration of Muscular Layer of Urinary Bladder Wall
The Malaysian Journal of Medical Sciences (MJMS) is a peer-reviewed, open-access journal published online at least six times a year. It covers all aspects of medical sciences and prioritizes high-quality research.
This page presents a generated summary with additional references; See source (below) for actual content.
Original source:
This page is merely a summary which is automatically generated hence you should visit the source to read the original article which includes the author, publication date, notes and references.
Salah Abood SALEM, Angela Ng Min HWIE, Aminuddin SAIM, Christopher Ho CHEE KONG, Ismail SAGAP, Rajesh SINGH, Mohd Reusmaazran YUSOF, Zulkifili MD ZAINUDDIN, Ruszymah HJ IDRUS
The Malaysian Journal of Medical Sciences:
(A peer-reviewed, open-access journal)
Full text available for: Human Adipose Tissue Derived Stem Cells as a Source of Smooth Muscle Cells in the Regeneration of Muscular Layer of Urinary Bladder Wall
Year: 2013
Copyright (license): CC BY 4.0
Download the PDF file of the original publication
Summary of article contents:
Introduction
Smooth muscle is integral to the function of various human organs, particularly in the urinary bladder, where its health is critical for proper capacity and compliance. Recent advancements in regenerative medicine and tissue engineering have focused on utilizing stem cells for repair and regeneration. Adipose-derived stem cells (ADSCs), which can be easily harvested from adipose tissue, present a promising alternative to bone marrow stem cells due to their abundance and reduced donor morbidity. This study aimed to determine whether ADSCs can differentiate into smooth muscle cells, potentially aiding in the regeneration of damaged bladder tissues.
Differentiation of ADSCs into Smooth Muscle Cells
The research demonstrated that ADSCs could be effectively induced to differentiate into smooth muscle cells (SMCs) using a smooth muscle inductive medium (SMIM). The experiment involved cultivating ADSCs in vitro and subsequently treating them with SMIM for three to six weeks. Characterization of the differentiated cells showed significant morphological changes, with the cells becoming elongated and spindle-shaped, typical of smooth muscle. Additionally, gene expression analysis via PCR confirmed the presence of smooth muscle-specific markers, such as alpha smooth muscle actin (ASMA) and myosin heavy chain (MHC), indicating successful differentiation. Protein expression levels also aligned with these findings, reinforcing the potential of ADSCs to serve as a source for regenerating smooth muscle.
Conclusion
This study concludes that adipose-derived stem cells hold significant promise as precursors for smooth muscle repair, particularly in the context of urinary bladder regeneration. The successful differentiation of ADSCs into smooth muscle cells, evidenced by both morphological and molecular changes, underscores their potential application in regenerative therapies. Future research could further explore their clinical implementation, providing innovative solutions for conditions affecting bladder smooth muscle along with other organ systems reliant on smooth muscle integrity.
FAQ section (important questions/answers):
What is the main objective of the study on ADSCs?
The study aims to confirm that adipose-derived stem cells (ADSCs) can be differentiated into smooth muscle cells, providing a potential source for regeneration strategies in urinary bladder smooth muscle repair.
How were ADSCs isolated and cultured in the study?
ADSCs were isolated from subcutaneous adipose tissue using 0.075% collagenase, followed by in vitro culture and expansion. After two passages, they were induced to differentiate using smooth muscle inductive media for three and six weeks.
What markers indicate successful differentiation of ADSCs into smooth muscle cells?
The study used smooth muscle markers such as alpha smooth muscle actin (ASMA), calponin, and myosin heavy chain (MHC) to confirm differentiation at both gene and protein expression levels.
What conclusions were drawn about the use of ADSCs?
The findings suggest that ADSCs can differentiate into smooth muscle precursor cells, representing a promising alternative for urinary bladder smooth muscle repair, leveraging their easy accessibility and multipotential.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Adipose-Derived Stem Cells for Urinary Bladder Smooth Muscle Regeneration’. Further sources in the context of Science might help you critically compare this page with similair documents:
Adipose Tissue, Urinary bladder, Regenerative medicine, Gene expression, Clinical application, Cell culture, RT-PCR, Cell differentiation, Cell morphology, Phenotypic expression, Cell therapy, Tissue engineering, Protein expression level, Growth factor, Immunocytochemistry, Multipotent Stem Cells, Stem cell, Adipose-derived stem cells, Protein expression, Biomaterial, Smooth muscle differentiation, Protein level, Smooth muscle, Smooth muscle cells, Differentiation potential, Genetic expression, Tissue engineering technique.