Effect of Heated Palm Olein on Blood Pressure and Lipid Peroxidation

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Journal name: The Malaysian Journal of Medical Sciences
Original article title: Effect of Repeatedly Heated Palm Olein on Blood Pressure—Regulating Enzymes Activity and Lipid Peroxidation in Rats
The Malaysian Journal of Medical Sciences (MJMS) is a peer-reviewed, open-access journal published online at least six times a year. It covers all aspects of medical sciences and prioritizes high-quality research.
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Original source:

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Author:

Leong Xin-Fang, Salimon Jumat, Mustafa Mohd Rais, Jaarin Kamsiah


The Malaysian Journal of Medical Sciences:

(A peer-reviewed, open-access journal)

Full text available for: Effect of Repeatedly Heated Palm Olein on Blood Pressure—Regulating Enzymes Activity and Lipid Peroxidation in Rats

Year: 2012

Copyright (license): CC BY 4.0


Download the PDF file of the original publication


Summary of article contents:

Introduction

Oxidative stress is a crucial factor influencing the development of cardiovascular diseases, and this study investigates the effects of heated palm olein on blood pressure-regulating enzymes and lipid peroxidation. As palm oil remains a popular cooking oil due to its resistance to oxidation during frying, understanding the impact of repeated heating is essential as it is commonly reused in food preparation. This research examined the biochemical effects of fresh versus repeatedly heated palm olein, focusing on its impact on angiotensin-converting enzyme (ACE) levels, heme oxygenase (HO) activity, and lipid peroxidation in Sprague-Dawley rats over a six-month period.

Increased Lipid Peroxidation and Blood Pressure-Regulating Enzyme Activity

The study found that repeatedly heated palm olein significantly increased peroxide levels, ACE activity, and lipid peroxidation while reducing heme oxygenase activity. Specifically, rats fed diets supplemented with palm olein heated multiple times exhibited higher levels of thiobarbituric acid reactive substances (TBARS), an indicator of lipid peroxidation, compared to those receiving fresh oil or oil heated just once. Additionally, heme oxygenase activity was notably decreased in the heated oil groups, with the most pronounced effects observed in the group consuming palm olein heated ten times. These findings suggest that repeated heating of palm olein not only compromises its nutritional value but could also contribute to elevated blood pressure and other cardiovascular risks linked to increased oxidative stress.

Conclusion

In conclusion, the consumption of thermally oxidised palm olein adversely affects the activity of essential enzymes involved in blood pressure regulation, leading to heightened levels of ACE and reduced activity of HO. These changes are associated with increased lipid peroxidation, indicating that the nutritional integrity of palm olein is compromised due to repeated heating. Consequently, it is recommended that palm olein not be reheated more than once to avoid its potentially harmful effects on health. This study highlights the importance of considering cooking practices in dietary habits to mitigate health risks associated with oxidative stress and hypertension.

FAQ section (important questions/answers):

What impact does repeated heating of palm olein have on health?

Repeatedly heated palm olein may negatively affect blood pressure-regulating enzymes, increasing angiotensin-converting enzyme levels while decreasing heme oxygenase activity, which can contribute to hypertension and elevated lipid peroxidation.

How does heating palm olein affect its chemical properties?

Heating palm olein increases peroxide levels significantly, indicating oxidative degradation. The peroxide value rises with each heating cycle, affecting oil stability and encouraging lipid peroxidation, which can be detrimental to health.

What are the main findings regarding lipid peroxidation from the study?

The study found that heated palm olein markedly increased lipid peroxidation levels, measured by elevated thiobarbituric acid reactive substances (TBARS), indicating increased oxidative stress associated with consuming such oil.

What role does heme oxygenase play in blood pressure regulation?

Heme oxygenase-1 (HO-1) is crucial for modulating blood pressure, functioning as an antioxidant. Its activity helps to control vascular tone, while a decrease in HO-1 levels can lead to hypertension.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Effect of Heated Palm Olein on Blood Pressure and Lipid Peroxidation”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Study (Studying):
A systematic investigation aimed at discovering knowledge, addressing hypotheses, and drawing conclusions under controlled conditions. In the context of the provided text, the study evaluates the effects of heating palm olein on blood pressure-regulating enzymes, lipid peroxidation, and overall health outcomes in animal models, thereby contributing to nutrition and health sciences.

2) Blood:
A vital fluid in the body that circulates through the cardiovascular system, delivering oxygen and nutrients to cells while removing waste products. The study assesses blood samples to analyze the activity of enzymes related to blood pressure regulation and to measure oxidative stress indicators, contributing to insights on cardiovascular health.

3) Food:
Nutritional substances consumed to sustain life, promote growth, and provide energy. The cooking oil used in the study, particularly palm olein, is scrutinized to evaluate its safety and health impact when heated multiple times during cooking, emphasizing the dietary implications on cardiovascular risks.

4) Activity:
Refers to the biochemical functions and behaviors of enzymes or metabolic processes. In the study, it pertains to measuring the activity of angiotensin-converting enzyme and heme oxygenase, which are pivotal in regulating blood pressure and responding to oxidative stress within the body.

5) Animal:
Refers to living organisms used in experimental studies to model human physiology and disease. The study employs male Sprague-Dawley rats, chosen for their physiological relevance to human health research, to investigate the effects of heated palm oil on cardiovascular and enzymatic functions.

6) Heating:
The application of heat to materials, which in this study pertains to cooking oils. Heating palm olein alters its chemical composition, introduces free radicals, and affects lipid profiles and enzyme activities, thus examining the implications of reheating oils on health factors such as blood pressure.

7) Diet:
A habitual intake of food and drink for nourishment, which influences health. The study investigates specific diets enriched with different forms of palm olein to observe effects on biochemical markers related to blood pressure and oxidative stress, thereby linking diet to cardiovascular disease.

8) Disease:
A pathological condition caused by various factors, leading to physiological dysfunction. The study addresses diseases linked to hypertension and cardiovascular issues, examining how dietary habits, particularly the consumption of heated cooking oils, influence the mechanisms leading to these diseases.

9) Substance:
A specific material with distinct physical and chemical properties. In the context of the study, substances such as palm olein, its heated forms, and various biochemical markers (like malondialdehyde) are analyzed for their impact on health, supporting discussions on dietary toxicology.

10) Table:
A systematic arrangement of data. In the research document, tables are used to effectively present quantitative findings, such as fatty acid compositions and peroxide values of various palm olein treatments, aiding in the comparison of treated vs. untreated oils in the study.

11) Rich (Rch):
Describing a high concentration of a particular element or compound. This term applies to the composition of palm olein, which is noted for its balanced fatty acids and antioxidant-rich components, emphasizing the nutritional value of fresh oil compared to its repeatedly heated counterparts.

12) Measurement:
Quantitative assessment of variables. The study employs various measurement techniques, including gas chromatography and enzyme activity assays, to gauge the effects of dietary forms of palm olein, ensuring reliable data collection for analyzing health impacts.

13) Water:
A fundamental solvent and component in biological systems. Though not directly tested, water is mentioned regarding the physiological environment of the study subjects. This element is crucial for maintaining hydration and enabling biological reactions essential to the study's metabolic assessments.

14) Hand:
Refers to manual dexterity or the personal involvement of researchers. In scientific studies, hands-on methodologies are vital for conducting experiments, managing animal subjects, and performing analyses, which play a critical role in the integrity and accuracy of research findings.

15) Post:
Typically refers to subsequent events or publishing findings. In research, post-experimental analysis is crucial to draw conclusions about data collected and communicate outcomes in academic or professional forums, as demonstrated by the study’s implications on cooking oil safety.

16) Male:
Specifies the sex of the experimental subjects used in the study, male Sprague-Dawley rats. The implications of using a single sex in studies are essential for hormonal and physiological consistency, affecting the generalizability of findings to human health.

17) Pharmacological:
Relating to the study of drugs and their effects on biological systems. This term is relevant as the study examines the biochemical effects of heated oils, linking dietary constituents to their pharmacological impact on health through influencing enzyme activity.

18) Pharmacology:
The science of drugs and their interactions with living systems. The study connects findings about dietary oils with pharmacological principles, as oxidative stress and altered enzyme activity can inform therapeutic strategies against hypertension and other cardiovascular diseases.

19) Inflammation:
A biological response to harmful stimuli, which can contribute to various chronic diseases. Although focused on lipids and enzymes, the study hints at the role of repeated heating of oils in fostering oxidative stress that may lead to inflammatory processes impacting cardiovascular health.

20) Performance:
Refers to the effectiveness or efficiency of an entity's function. The study evaluates the performance of blood pressure-regulating enzymes in response to the consumption of both fresh and repeatedly heated palm olein, contributing to understanding health implications.

21) Observation:
The act of monitoring and recording phenomena. In scientific studies, careful observation of experimental results is essential for validating hypotheses—such as the effects of heated palm olein on rat physiology and enzyme activity, forming the basis for conclusions drawn.

22) Similarity:
Refers to the common features shared among subjects or substances. The comparison of results across different groups, such as fresh vs. heated oils, highlights the significance of identifying similarities and differences in their biochemical impact on health variables.

23) Quality:
Refers to the standard or grade of a substance. In the study, the quality of cooking oil—assessed by its fatty acid composition, peroxide levels, and overall stability—is critical in determining its health implications, particularly in relation to cardiovascular risks.

24) Species:
Refers to a distinct group of organisms that share common characteristics and can reproduce. The use of the Sprague-Dawley rat as a model species is relevant in studies like this for their physiological and genetic similarities to humans pertaining to health research.

25) Science (Scientific):
A systematic enterprise that builds and organizes knowledge in the form of testable explanations. The study embodies scientific inquiry by employing experimental designs to understand the health implications of dietary practices on blood pressure and oxidative stress.

26) Surface:
The outermost layer of a material. Surface chemistry is important in the context of frying oils as it relates to reactions occurring at oil and food interfaces, where heated oils react with food components, significantly affecting nutritional quality.

27) Reason:
Refers to the rationale behind actions or conclusions. The study provides reasons—based on biochemical evidence—why the consumption of repeatedly heated palm olein may detrimentally impact health, thereby offering a scientific basis for dietary recommendations.

28) Street:
Relates to common practices in food preparation. The study highlights the use of cooking oils in street food settings, emphasizing societal impact on dietary habits and the potential health risks associated with frequently reheated oils consumed by the public.

29) Powder:
Refers to finely crushed substance. In the context of dietary studies, the use of powdered rat chow in the study provides a controlled method of delivering nutrients and ensuring consistent feeding, essential for accurate research outcomes.

30) Gupta:
References a specific researcher, highlighting contributions to the field. In the provided context, Gupta's works have been cited to support findings related to cooking oils and their impact on health, showing the collaborative nature of scientific research.

31) Trade:
Refers to the buying and selling of commodities, in this context emphasizing the economic aspect of palm oil production and its significance in global markets, directly influencing dietary practices and health implications on a population level.

32) Cage:
Refers to the enclosure where experimental animals are housed. The conditions of the cages, including temperature and light cycles, are vital for ensuring the well-being of the animals during the study, affecting the validity of the experimental data.

33) Gold (Golden):
Used in naming a specific brand of rat chow, referring to quality nutrition. Gold is associated with high standards in research diets, ensuring that nutritional compositions support valid experimental outcomes in the study of palm olein effects.

34) Drug:
A substance used for medical treatment or therapeutic effect. In this context, while palm oil is not a drug, its effects on blood pressure-regulating enzymes relate to pharmacology, emphasizing dietary influences akin to medicinal interventions in health.

35) Rati:
An abbreviation or context wherein it may refer to radiological studies or imaging. In this context, however, it may represent the radiance of findings that support health implications resulting from dietary practices observed in the study, underscoring the need for future research.

36) Life:
A biological state characterized by growth, reproduction, and adaptation. In the context of the study, it refers to health outcomes directly linked to dietary choices, highlighting the importance of understanding how food impacts overall well-being and longevity.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Effect of Heated Palm Olein on Blood Pressure and Lipid Peroxidation’. Further sources in the context of Science might help you critically compare this page with similair documents:

Heated oil, Food preparation, Heating process, Nutrition, Chemical reaction, Blood-pressure, Control group, Nutritional value, Antioxidant properties, Experimental group, Oxidative stress, LDL cholesterol, Reactive oxygen species, Gastrointestinal system, Cardiovascular disease, Endothelial dysfunction, Lipid peroxidation, Hypertension, Oxidative damage, Antioxidant Effect, Peroxide value, Physicochemical properties, Coronary artery disease, Antioxidant, Experimental rats, Vascular disease, Malondialdehyde (MDA), Nitric oxide, Insulin sensitivity, Malondialdehyde, Angiotensin converting enzyme, Ang II, Chemical stability, Cell proliferation, Experiment group, Statistical analyses, High temperature, Cardiac failure, Biological significance, Systemic circulation, Plasma samples, Hypercholesterolemia, Feedback mechanism, Cooking oil, Vascular smooth muscle, Endothelial function, Renin-angiotensin system, Fatty Acid Composition, Vasodilatation, Protein content, Free Radical, Toxic products, Basal diet, Oxidative rancidity, Biochemical analyses, Thiobarbituric acid reactive substance, Vitamin E, Palm oil, Experimental animal, Proinflammatory cytokine, Thiobarbituric Acid, Fatty acid, Unsaturated fatty acid, Angiotensin II, Vascular function, Cell injury, Cytoprotective effect, MDA level, Vascular tone, Oxidized LDL, Ferrous ion, Organoleptic qualities, Antioxidant function, Antioxidant system, Obesity impact, Endothelial cell injury, Carbon monoxide, Nicotinamide adenine dinucleotide, Heme oxygenase-1, Vascular Dysfunction, Health effect, Superoxide production, LDL cholesterol level, Deep-fat frying, Aldehydes, Fried food, Vasorelaxation, Blood vessel, Endothelium, Ox - LDL, Lipid peroxidation level, Polyunsaturated fatty acid, Saturated fatty acid, Frying process, Percentage increase, Monounsaturated fatty acid, Peroxynitrite.

Concepts being referred in other categories, contexts and sources.

Ace, Heating, Repeated heating, Physical reaction, Endothelial cell.

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