Construction of an artificial recombinant bicistronic plasmid DNA vaccine...

[Full title: Construction of an artificial recombinant bicistronic plasmid DNA vaccine against porcine rotavirus]

Original Research doi:10.4102/ojvr.v 80 i 1.498 http://www.ojvr.org Construction of an artificial recombinant bicistronic plasmid DNA vaccine against porcine rotavirus Authors: Tingting Cui 1 Jun Xiong 1 Yongzhi Wang 2 Xintian Wen 1 Xiaobo Huang 1 Yong Huang 1 Xiaoping Ma 1 Zhongkai Hu 1 Qin Zhao 3 Sanjie Cao 1 Affiliations: 1 College of Veterinary Medicine and Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, China 2 Sichuan Rural Science and Technology Development Centre, Chengdu, China 3 College of Food Science, Sichuan Agricultural University, China Correspondence to: Sanjie Cao Email: csanjie@gmail.com Postal address: 46 Xinkang Road, Ya’an 625014, China Dates: Received: 27 June 2012 Accepted: 31 Oct. 2012 Published: 04 Mar. 2013 How to cite this article: Cui, T., Xiong, J., Wang, Y., Wen, X., Huang, X., Huang, Y. et al ., 2013, ‘Construction of an artificial recombinant bicistronic plasmid DNA vaccine against porcine rotavirus’, Onderstepoort Journal of Veterinary Research 80(1), Art. #498, 8 pages. http://dx.doi. org/10.4102/ojvr.v 80 i 1.498 Copyright: © 2013. The Authors Licensee: AOSIS OpenJournals. This work is licensed under the Creative Commons Attribution License The attenuated Salmonella typhimurium χ 4550 strain was used to harbour a reconstructed bicistronic DNA vaccine against porcine rotavirus, which carried the rotavirus nonstructural protein 4 ( NSP 4 ) and VP 7 genes simultaneously. Using a balanced lethal system, the kanamycin resistance gene of expressing eukaryotic plasmids pVAX 1 and pVAXD were replaced by the aspartate β -semialdehyde dehydrogenase ( asd ) gene. The NSP 4 cleavage product (259–525) of rotavirus OSU strain and VP 7 full-length genes were amplified by reverse transcription polymerase chain reaction and then inserted into the eukaryotic single-expression plasmid, pVAX 1-asd, and the eukaryotic dual-expression plasmid, pVAXD-asd, respectively. The recombinant plasmids pVAX 1-asd-NSP 4, pVAX 1-asd-VP 7 and pVAXD-asd-NSP 4-VP 7 were transformed into the attenuated S. typhimurium χ 4550 strain by electrotransformation. An indirect immunofluorescence assay of the expressed COS-7 cell suggested that the recombinant S. typhimurium χ 4550 strain was constructed successfully. The recombinant S.typhimurium χ 4550 strain was orally administered to BALB/c mice. The group immunised with dualexpression plasmids produced a significantly higher level of serum Immunoglobulin G (IgG) and intestinal Immunoglobulin A (IgA) than the group immunised with single-expression plasmids. These results indicated that eukaryotic bicistronic plasmid DNA vaccines could be successfully constructed to enhance humoural, mucosal and cellular immune response against rotavirus infection. Introduction Rotavirus is a highly infectious, triple-layered icosahedral, non-enveloped virus particle with a genome of 11 segments of double-stranded Ribonucleic acid (RNA) (Petrie, Estes & Graham 1983). It is recognised as the most important cause of acute gastroenteritis and dehydration in young children and animals (Estes 2001). Although highly effective, currently available reassortant tetravalent rhesus rotavirus vaccines were found to cause a higher occurrence of intussusception in young children (Glass et al . 2006). Moreover, the safe rotavirus-like particle (VLP) vaccines induce only partial protection and intestinal antibody secreting cell responses when used in conjunction with live attenuated human rotavirus (Gonzalez et al . 2004; Yuan et al . 2001) and 2/6-VLP vaccines do not protect suckling mouse pups from rotavirus diarrhoea (Coste et al . 2000). Thus, effective protective rotavirus vaccines that are unlikely to cause intussusception would be highly desirable. The use of DNA vaccines is a new approach to protect animals and children against rotavirus. VLP and DNA vaccines may constitute a third generation of rotavirus vaccines (Chen et al . 1998; O’Neal et al . 1997). It was previously reported that parenteral immunisation with rotavirus VP 4, VP 6 and VP 7 DNA vaccines induce high levels of serum rotavirus Immunoglobulin G (IgG) but fail to protect mice against viral challenge (Chen et al . 1997; Herrmann et al . 1999; Yang et al . 2001). It is therefore necessary to develop an efficient method to increase the immunogenicity of DNA vaccines The rotavirus nonstructural protein 4 (NSP 4) is the first described viral enterotoxin and may inducing doseand age-dependent diarrhoea in neonatal mice without causing histological alterations (Ball et al . 1996). When mice were infected with rotavirus, NSP 4-specific Immunoglobulin A (IgA) antibodies were generated in response to mucosal immunisation and transcytosed from the basal lamina into the mucosal epithelium (Gebert 1997). Further, orally immunised mice produced maternal antibodies against NSP 4, generating passive protection in newborn mouse pups against rotavirus challenge (Ward, Rich & Besser 1996). Thus, NSP 4 is thought to be a promising candidate for a rotavirus DNA vaccine that induces a significant and effective immune response (Ball et al . 2005). VP 7 is a structural protein of the outer capsid of a rotavirus and is encoded in the seventh, eighth or ninth gene segment of the genome (depending on the viral strain) (Song & Hao 2009). VP 7 may be the dominant immunogen for the production of neutralising antibodies, which are thought to have an important role in the protection against rotavirus-induced diarrhoea (Ward et al . 1990, 1993) and associate resistance to protect the host against diarrhoea upon challenge with the virulent Page 1 of 8 Scan this QR code with your smart phone or mobile device to read online Read online:

Original Research doi:10.4102/ojvr.v 80 i 1.498 http://www.ojvr.org parental rotaviruses (Hoshino et al . 1988). Matsui et al. (1989) demonstrated that VP 7 monoclonal antibodies (MAbs) have two distinct epitopes, with the specific epitopes defined by VP 7-specific MAb 57-8 mediating protection against at least two rotavirus serotypes. From this we concluded that VP 7 plays an important role in resisting rotavirus challenge In this study, a bicistronic plasmid was formed as a coexpression vector carrying the C-terminal of the porcine rotavirus (PRV) NSP 4 and VP 7 genes, and then inserted into the Salmonella typhimurium χ 4550 strain as a DNA vaccine that was orally administered to BALB/c mice. The S. typhimurium χ 4550 strain consists of attenuated bacteria of which the asd gene has been deleted. We evaluated the mucosal and systemic antibody responses. The results showed that the coexpression DNA vaccine mediated by a bicistronic plasmid could significantly enhance humoural and mucosal immune response Materials and method Bacterial strains, plasmid, virus, cell lines and experiment mice The attenuated S. typhimurium χ 6212, χ 3730 and χ 4550 strains and plasmid PYA 3342 were kindly provided by Professor Kai Schulze of the Helmholtz Centre for Infection Research, Braunschweig, Germany. The eukaryotic expression vector pVAX 1 was purchased from Invitrogen, Carlsbad. It ‘contains cytomegalovirus (CMV) immediate-early promoter for efficient expression and bovine growth hormone (BGH) poly A signal for mRNA stability’ (Yang et al . 2009). The OSU rotavirus strain was used to generate cDNA and produce virus antigen stock, which was applied as the antigen in the enzyme-linked immunosorbent assays (ELISAs). MA 104 cells and COS-7 cells were purchased from the China Center for Type Culture Collection (Wuhan, China). Six-weekold inbred BALB/c female mice were obtained from the Chengdu Institute of Biological Products (Chengdu, China) Construction and reforming of the eukaryotic bicistronic expression vector pVAXD-asd The vectors pVAXA and pVAXB were constructed by reforming the multiple cloning site of pVAX 1. The product of pVAXA polymerase chain reaction (PCR) (-PCMV- MCS-BGHpolyA-) was linked with pVAXB to form vector pVAXD with two different multiple cloning sites. Dualenzyme digestion identification showed that the bicistronic vector pVAXD was successfully constructed. The aspartate β -semialdehyde dehydrogenase ( asd ) gene was amplified by PCR and digested by restriction enzymes BamHI and XbaI from plasmid PYA 3342. We then used the Klenow fragment enzyme character to fill the sticky end of DNA fragments derived by enzyme restriction digestion to a flat end, and linked it with pVAX 1 and pVAXD vectors that were removed from the 5’ terminal phosphate group by calf intestinal alkaline phosphatase. The reformed plasmids, pVAX 1-asd and pVAXD-asd, were reconstructed successfully and plasmid pVAXD-asd was sequenced by Invitrogen, Shanghai Construction of vaccine plasmid and its transformation into attenuated Salmonella typhimurium Viral RNA was extracted from MA 104 cells infected with the OSU rotavirus strain by using the RNAsimple Total RNA Kit (TIANGEN, Beijing). Total cDNA was obtained from the viral total RNA by a reverse transcriptase reaction procedure. The NSP 4 segment was amplified from the cDNA using the sense primer S 1 5’-CTGCAGCCGCCATGGAACAGGTTACTAC-3’ (forward) and the antisense primer 5’-CTCGAGCTCACATA GACGCAGTTACTTCCGAC-3’ (reverse). Underlined areas indicate the PstI and XhoI sites, respectively. The VP 7 segment was amplified from the cDNA using the sense primer S 2 5’-GCTAGCCGCCATGTATGGTATTGAAT-3’ (forward) and the antisense primer 5’-GGTACCATCTAGACTCGGT AATAAAAGGCAG-3’ (reverse). Underlined areas indicate the NheI and KpnI sites, respectively. The amplified DNA fragments of the NSP 4 and VP 7 genes were cloned into the pMD 19-T vector (TaKaRa) and sequenced. The NSP 4 fragment was released from the pMD 19-T plasmid by PstI and XhoI digestion and ligated into the reformed pVAX 1-asd plasmid. pVAXD-asd vectors were cut by the same enzymes. The recombinant plasmids were named pVAX 1-asd-NSP 4 and pVAXD-asd-NSP 4. The VP 7 fragment was subsequently released from the pMD 19-T plasmid by NheI and KpnI digestion and ligated into the pVAX 1-asd and pVAXD-asd- NSP 4 vectors, cut by the same enzymes. The recombinant plasmids were named pVAX 1-asd-VP 7 and pVAXD-asd- NSP 4-VP 7. These plasmids, including pVAX 1-asd-VP 7, pVAX 1-asd-NSP 4, pVAXD-asd-NSP 4-VP 7 and pVAX 1-asd, were electroporated into S. typhimurium χ 3730 with a cuvette (1.8 kV, 200 Ω , 25 μ F) Positive transformants were electroporated into S. typhimurium χ 4550 using the same method as described above. Lastly, the positive transformants were verified by PCR amplification and digested with restriction enzymes. The resulting strains were named χ 4550 (pVAX 1-asd-VP 7), χ 4550 (pVAX 1-asd-NSP 4), χ 4550 (pVAXD-asd-NSP 4-VP 7) and χ 4550 (pVAX 1-asd) Transfection of COS-7 cells and transient expression COS-7 cells were seeded in six wells of tissue culture plates (Costar, Shanghai). Cells were transfected with the recombinant plasmids pVAX 1-asd-VP 7 in the first well, pVAX 1-asd-NSP 4 in the second well, pVAXD-asd-NSP 4-VP 7 in the third and fourth wells, and pVAX 1-asd in the fifth and sixth wells according to the instructed protocol provided by the supplier (Invitrogen Life Technologies, Carlsbad). Cells were washed with phosphate buffered saline (PBS) after 36 hours, then fixed with an ice-cold methanol and acetone mixture (1:1) at 4 °C for 30 min and washed again with PBS. Two kinds of diluted primary antibody were collected from rabbits immunised by prokaryotic express protein VP 7 and NSP 4, respectively, and incubated at 37 °C for 1 h. Diluted secondary antibodies (FITC-conjugated goat anti-rabbit IgG) were then added to all groups and plates were incubated at 37 °C for 1 h Page 2 of 8

Original Research doi:10.4102/ojvr.v 80 i 1.498 http://www.ojvr.org Page 3 of 8 Detection of the transcription of NSP 4 and VP 7 genes in vivo Six-week-old mice were orally inoculated with 1 × 10 9 CFU of χ 4550 (pVAXD-asd-NSP 4-VP 7). Three days post immunisation, Payer’s patches were removed from three mice and pooled. Cellular RNA was isolated from homogenised Payer’s patches with Trizol (TaKaRa, Dalian) according to the manufacturer’s instructions. The transcripts of PRV NSP 4 and VP 7 genes in Payer’s patches were analysed by means of reverse transcription (RT) PCR using specific primers S 1 and S 2 described earlier (Yang et al . 2009) Stability and safety of recombinant attenuated Salmonella typhimurium A single bacterial colony of the selected recombinant χ 4550 (pVAXD-asd-NSP 4-VP 7) strain was inoculated into antibiotic-free Luria-Bertani (LB) liquid medium and incubated at 37 °C, 200 r/min until the optical density of the culture reached approximately 0.6 at 600 nm. We then inoculated the bacterium (1:50) into antibiotic-free LB liquid medium and incubated samples at 37 °C, 200 r/min. Over the next 18 hours, at three-hour intervals, the bacterium was transferred from the LB liquid medium onto LB agar medium and identified by RT-PCR with specific primer S 2. A gavage needle was used to inoculate six-week-old BALB/c mice (groups of three) intragastrically with χ 4550 (pVAXD-asd- NSP 4-VP 7) at a dosage of 1×10 8 CFU, 1×10 9 CFU and 5×10 9 CFU. They were boosted with the same dosages two weeks later. At the same time, the control group was given PBS at the same dosages. The health of the mice was observed every day for 30 days Inoculation of mice Eight-week-old BALB/c mice were randomly assigned to six groups of 15 each: • Group A: PBS (pH 7.2) • Group B: χ 4550 (pVAX 1-asd) • Group C: χ 4550 (pVAX 1-asd-NSP 4) • Group D: χ 4550 (pVAX 1-asd-VP 7) • Group E: χ 4550 (pVAXD-asd-NSP 4-VP 7) • Group F: χ 4550 (pVAX 1-asd-NS 4)+ χ 4550 (pVAX 1-asd- VP 7) On day 1 mice of groups B, C, D and E were orally immunised with 0.2 mL Salmonella suspension (1 × 10 9 CFU) and boosted with the same dosage twice at two-week intervals. The coimmunised group (F) received the same dosage of two equally mixed Salmonella suspensions (1 × 10 9 CFU) on days 1, 14 and 28. The control group (A) received 0.2 mL PBS on days 1, 14 and 28. Collection and analysis of samples Three mice from each group were sacrificed for sera and intestinal lavage on days 1, 14 and 28. Blood was collected from the submaxillary sinuses of the mice and sera were prepared and stored at –20 °C until use. The intestinal fluid of each mouse was prepared by dicing the entire small intestine, excluding fat and the pancreas, and triturating it in 5 mL PBS containing 1 mmol/L phenylmethylsulfonyl fluoride (PMSF). To remove cellular debris, samples were centrifuged at 12 000 × g for 30 min. The supernatant was collected as resultant intestinal lavage and stored at –20 °C until use For measurement of rotavirus serum IgG and intestinal IgA levels in immunised mice, an indirect ELISA was used as described previously (Yang et al . 2009). Data of serum IgG and intestinal IgA titres were analysed using one-way analysis of variance. Significant differences were found amongst all immunisation groups ( p ≤ 0.05) Measurement of interferon- γ production Spleens of three mice from group E were removed aseptically and pooled into 10 mL RPMI 1640 medium 14 days after the third immunisation. Splenocytes were isolated by using a cell constrainer and centrifuged at 1000 rpm for 10 min at room temperature. The cell pellet was re-suspended in 10 mL of the mentioned medium and the concentration of cells was adjusted to 5 × 10 6 cells/mL. An aliquote of 2 mL of this cell suspension and 20 m g of stimulant protein NSP 4 and VP 7 equivalent admixture were added to each well of a 24-well pate. Plates were incubated in CO 2 at 37 °C for 72 h. The splenocyte culture supernatants were collected for interferon- γ (IFN- γ ) analysis using an IFN- γ ELISA kit according to the manufacturer’s instructions (Fangcheng Biotech, Beijing) Ethical considerations Animals were handled humanely during sample collection under the supervision of veterinarians and according to procedures complying with Chinese laws Results Reforming eukaryotic expression vector pVAX 1-asd and pVAXD-asd The asd segments with flat ends were ligated into plasmid pVAX 1 and pVAXD and then heat-shock transformated into χ 6212 competent cells. Fragments of 1400 base pairs (bp) were amplified by RT-PCR from a single bacterial colony, indicating that the vectors pVAX 1-asd and pVAXD-asd were reformed successfully (Figure 1). Construction of the transient expression of vaccine plasmid pVAX 1-asd-VP 7, pVAX 1-asd- NSP 4, pVAXD-asd-NSP 4-VP 7 A fragment of 300 bp was amplified by RT-PCR from the PRV OSU strain. Sequence analysis showed that the amplified sequence was 267 bp in length and encoded 89 amino acids. The result of sequence alignment showed that the predicted amino acid sequence exhibited 97.8 % homology with the sequence of the NSP 4 gene fragment (87–175) of the PRV OSU strain. The NSP 4 gene fragment was inserted into the pVAX 1- asd and pVAXD-asd vectors. Another 1000-bp fragment was

Original Research doi:10.4102/ojvr.v 80 i 1.498 http://www.ojvr.org Page 4 of 8 amplified by RT-PCR from the PRV OSU strain. Sequence analysis showed that the amplified sequence was 987 bp in length and encoded 326 amino acids. The result of sequence alignment showed that the predicted amino acid sequence exhibited 99.4 % homology with the sequence of the VP 7 gene fragment of the PRV OSU strain. The VP 7 gene fragment was inserted into the pVAX 1-asd and pVAXD-asd-NSP 4 vectors. The recombinant plasmids pVAX 1-asd-NSP 4, pVAX 1-asd- VP 7 and pVAXD-asd-NSP 4-VP 7 were electroporated into S. typhimurium χ 4550 strain. They were subsequently detected by enzyme restriction analysis (Figures 2, 3 and 4). Transient expression of recombinant plasmids in COS-7 cells The expression of recombinant plasmids pVAX 1-asd- VP 7, pVAX 1-asd-VP 7 and pVAXD-asd-NSP 4-VP 7 was Column 1, DNA marker III; Column 2, products of recombinant pVAX 1-asd coenobium polymerase chain reaction identification; Column 3, products of recombinant pVAXD-asd coenobium polymerase chain reaction identification; bp, base pairs FIGURE 1: Identification electrophoresis of recombinant pVAX 1-asd and pVAXD-asd with coenobium polymerase chain reaction 1 2 3 4500 3000 2000 1200 800 500 200 1400 bp Column 1, DNA marker III; Column 2, pVAX 1-asd-NSP 4 digested with PstI and XhoI ; bp, base pairs. FIGURE 2: Identification electrophoresis of recombinant pVAX 1-asd-NSP 4 with restriction enzyme 1 2 4500 3000 2000 1200 800 500 200 3000 bp 300 Column 1, DNA marker III; Column 2, pVAX 1-asd-VP 7 digested with NheI and KpnI ; bp, base pairs FIGURE 3: Identification electrophoresis of recombinant pVAX 1-asd-VP 7 with restriction enzyme 1 2 4500 3000 2000 1200 800 500 200 3000 bp 1000 Column 1, DNA marker III; Column 2, pVAXD-asd-NSP 4-VP 7 digested with PstI and XhoI ; Column 3, pVAXD-asd-NSP 4-VP 7 digested with NheI and KpnI ; Column 4, pVAXD-asd-NSP 4- VP 7 digested with PstI ; bp, base pairs FIGURE 4: Identification electrophoresis of recombinant pVAXD-asd-NSP 4-VP 7 with restriction enzyme 1 2 3 4 4500 3000 2000 1200 800 500 200 bp bp

Original Research doi:10.4102/ojvr.v 80 i 1.498 http://www.ojvr.org Page 5 of 8 demonstrated by an indirect immunofluorescence assay. Cytoplasmic fluorescence was observed in COS-7 cells transfected with all the recombinant plasmids, but not with the parental vector pVAX 1 (Yang et al . 2009) (Figure 5). Transcription of recombinant plasmids in vivo using attenuated Salmonella typhimurium as transgenic vehicle Three days after intragastrically inoculating mice with attenuated S.typhimurium χ 4550 (pVAXD-asd-NSP 4-VP 7), Payer’s patches were removed from three mice and used for RNA extraction. Payer’s patches are not only the main colonisation site of attenuated S.typhimurium but also an important immunologically relevant site in the context of mucosal responsiveness. Two fragments of 300 bp and 1000 bp, respectively, were amplified by RT-PCR (Figures 6 and 7). Stability and safety of recombinant attenuated Salmonella typhimurium Selected single bacterial colonies of recombinant χ 4550 (pVAXD-asd-NSP 4-VP 7) were inoculated into antibiotic-free LB medium. The recombinant plasmids were subsequently identified by RT-PCR for 18 hours at three-hour intervals. The positive ratio reached 100 % , showing that the recombinant plasmid was of high stability. BALB/c mice that had been inoculated orally with χ 4550 (pVAXD-asd-NSP 4-VP 7) at different dosages were used for safety analysis. No adverse reactions were observed during the observation period; the diet, stools and health of the mice were all normal Production of serum anti-rotavirus IgG antibodies Following oral immunisation with all recombinant S. typhimurium vaccines, all mice developed detectable serum IgG titres against rotavirus. Serum anti-rotavirus IgG was measured by indirect ELISA using a 1:100 dilution of immunised mouse serum. As shown in Figure 8, the anti-rotavirus IgG was produced fast and appeared 28 days post inoculation. In group B and C, we could infer that the antibody level of VP 7 expression was significantly higher than NSP 4 expression ( p < 0.05). In addition, the coexpression of recombinant S. typhimurium χ 4550 (pVAXDasd-NSP 4-VP 7) produced the highest antibody level in group E, which was somewhat similar to that of group D and group F. No specific anti-rotavirus antibodies were observed in serum samples of group A (PBS) or group B ( χ 4550 [pVAX- FIGURE 5: Indirect immunofluorescence detection of the expression of recombinant plasmids in COS-7 cells. (a) Indirect immunofluorescence result of COS-7 cells transfected with pVAX 1-asd-VP 7; (b) Indirect immunofluorescence result of COS-7 cells transfected with pVAX 1-asd-NSP 4; (c) Indirect immunofluorescence result of COS- 7 cells transfected with pVAXD-asd-NSP 4-VP 7, by using anti-NSP 4 serum; (d) Indirect immunofluorescence result of COS-7 cells transfected with pVAXD-asd-NSP 4-VP 7, by using anti-VP 7 serum and (e) Indirect immunofluorescence result of COS-7 cells transfected with pVAX 1-asd, by using anti-VP 7 serum a c e b d Column 1, DNA marker III; Columns 2 and 3, transcription of VP 7 gene in vivo after mice were immunised orally with χ4550 (pVAXD-asd-NSP 4-VP 7) FIGURE 6: Detection of the transcription of the VP 7 gene in vivo after mice were immunised orally with recombinant Salmonella typhimurium (reverse transcription polymerase chain reaction) 1 2 3 4500 3000 2000 1200 800 500 200 1000 Column 1, DNA marker III; Columns 2 and 3, detection of the transcription of the NSP 4 gene in vivo after mice were immunised orally with χ4550 (pVAXD-asd-NSP 4-VP 7) FIGURE 7: Detection of the transcription of the NSP 4 gene in vivo after mice were immunised orally with recombinant Salmonella typhimurium (reverse transcription polymerase chain reaction) 1 2 3 4500 3000 2000 1200 800 500 200 bp 300 bp

Original Research doi:10.4102/ojvr.v 80 i 1.498 http://www.ojvr.org Page 6 of 8 asd]). The experiment demonstrated that the co-expression S. typhimurium vaccine may considerably enhance the humoural immune response Production of intestinal lavage anti-rotavirus IgA antibodies The ability of the recombinant S. typhimurium vaccine to induce a mucosal immune response was determined by detecting the level of IgA antibodies to rotavirus in intestinal lavage samples. As shown in Figure 9, the anti-rotavirus IgA was produced fast and first appeared after 14 days. A oneway analysis of variance indicated that there was not much difference amongst groups C, D, E and F, which received χ 4550 (pVAX 1-asd-NSP 4), χ 4550 (pVAXD-asd -VP 7), χ 4550 (pVAXD-asd-NSP 4-VP 7), χ 4550 (pVAX 1-asd-NSP 4) and χ 4550 (pVAXD-asd -VP 7), respectively. Up to day 28 post inoculation, there were significant differences amongst each group. The IgG antibody level of co-expression group E was the highest, that of group B and C was similarly low and for co-immunised group F the antibody level was notably higher than that of group C and D, but somewhat lower than for group E. No specific anti-rotavirus antibodies were observed in the intestinal lavage sample of group A (PBS) or group B ( χ 4550 [pVAX-asd]) Detection of interferon- γ secretion by splenocytes The secretion of IFN- γ by splenocytes in the supernatant of the cultures was detected by ELISA. A total of 1097.693 pg/mL IFN- γ was secreted by group E, which was much higher than that of the control group (group B) at 781.7125 pg/mL. This indicated that the immunity of mice was considerably increased post inoculation with the χ 4550 (pVAXD-asd- NSP 4-VP 7) vaccine Trustworthiness We declare that the experiments were conducted in a reliable, authentic and valid manner Discussion This experiment demonstrated that a bicistronic DNA vaccine that encodes the PRV VP 7 protein and the C-terminal of the NSP 4 protein simultaneously and is delivered by an attenuated S. typhimurium χ 4550 strain, was a potent vaccine, which induced a significantly higher level of serum IgG, mucosal IgA and splenocyte IFN- γ than in the mice immunised with a single-expression vaccine DNA immunisation is a considerable vaccination strategy, which has many desirable characteristics for an ideal vaccine, including induction of broad immune responses and longlasting immunity against diseases. In addition, oral delivery of plasmid DNA vaccines with bacteria as carriers is more efficacious than traditional DNA vaccines. Compared ‘to immunization with naked plasmid DNA, no further plasmid amplification and purification steps are needed, thereby reducing cost and labour extensively’ (Schoen et al . 2004). Oral immunisation with a rotavirus DNA vaccine was first demonstrated by Chen et al. (1997). Yang et al. (2001) reported that intramuscular immunisation of mice with VP 6 DNA vaccines induced high levels of VP 6-specific serum IgG and IgA antibodies but not fecal IgA antibodies. Our study provided evidence that attenuated S. typhimurium strain χ 4550 could be used as an oral delivery vector for PRV DNA OD 450, optical density at 450 nm FIGURE 8: Indirect enzyme-linked immunosorbent assay analysis of the antirotavirus IgG antibody in murine serum after immunisation with different vaccines 0.800 0.600 0.400 0.200 0.000 OD 450 A B C D E F Group 14 days 28 days 42 days OD 450, optical density at 450 nm FIGURE 9: Indirect enzyme-linked immunosorbent assay analysis of antirotavirus IgA antibody in murine serum after immunisation with different vaccines 0.800 0.600 0.400 0.200 0.000 OD 450 A B C D E F Group 14 days 28 days 42 days

Original Research doi:10.4102/ojvr.v 80 i 1.498 http://www.ojvr.org Page 7 of 8 vaccines. The target gene could be expressed both in vitro and in vivo to develop a specific humoural, mucosal and cellular immune response against PRV. Live bacteria as DNA vaccine vectors offer many potential advantages in contrast to inactivated or attenuated vaccines. They are able to deliver multiple antigens safely with easy oral immunisation. Bacteria naturally contain immunostimulatory molecules such as lipopolysaccharides, which can function as an adjuvant to provoke immune responses (Pasetti, Levine & Sztein 2003). Besides, Salmonella carrier strains have been shown to escape from the primary vacuole of the phagosome and transfer plasmids effectively to mammalian host cells in vivo (Schoen et al . 2004). Live Salmonella vectors have also been shown to induce a systemic immune response, including humoural, cellular and mucosal immunity against pathogen infection (Lintermans & Greve 1995) Safety is a prerequisite for plasmid DNA vaccines with live bacteria as vectors. In this study, we reconstructed the new plasmid by removing the kanamycin resistance gene and replaced it with the asd gene using a balanced lethal system. RT-PCR was used to ensure that the plasmids, pVAX 1-asd and pVAXD-asd, were reconstructed successfully. The asd gene is a diaminopimelic acid (DAP) biosynthetic gene, which is an important component of the cell wall of Gramnegative bacteria. As bacterial amino acids are not found in eukaryotes, no Salmonella could survive without DAP. An S. typhimurium χ 4550 strain without an asd gene was chosen as the plasmid DNA carrier as it was the only strain in which reconstructed plasmids could survive. During the experiment, the diet, stools and health of mice were all normal and no adverse reactions were observed. All the results suggested that the reconstructed S. typhimurium χ 4550 strain vaccine was safe. The structural protein VP 7 of a rotavirus constitutes the outer shell of the virion and elicits the production of distinct neutralising antibodies in the host. It is thought to play an important role in protecting rotavirus-induced diarrhoea. In previous research a murine model was constructed to express the rotavirus VP 7 protein with a transmissible gastroenteritis virus vector to immunise and provide protection against infections (Ribes et al . 2011). ‘Rotavirus-specific antibodies were found only after immunization by the intraperitoneal route. Partial protection against rotavirus-induced diarrhoea was achieved in suckling BALB/c mice born to dams immunized with the recombinant virus’ (Ribes et al . 2011) when they were orally challenged with the homotypic rotavirus. According to this study, improving the immunity of VP 7 was stringently demanded. Kavanagh et al . (2010) also demonstrated that the NSP 4 protein of a rotavirus has mucosal adjuvant properties, because it is a multifunctional protein that functions in rotavirus morphogenesis and pathogenesis and was the first viral enterotoxin to be described. This discovery correlates with the observation that inclusion of specific adjuvants in vaccines can modify the presentation modality of antigens to the immune system and improve the induction of the immune response over that induced by the same antigen given alone (Buonaguro, Tornesello & Buonaguro 2009 in Kavanagh et al . 2010). For these reasons, we reconstructed a bicistronic DNA vaccine harbouring the VP 7 and NSP 4 proteins synchronously. Both serum IgG and mucosal IgA antibody response against the rotavirus were detected post inoculation with the DNA vaccine. The vaccine expressing only the NSP 4 protein elicited a weak anti-rotavirus IgG and IgA antibody response. However, the vaccine expressing only the VP 7 protein induced a significantly stronger response, similar to that induced by co-immunised NSP 4 and VP 7 proteins. The bicistronic DNA vaccine co-expressing the VP 7 and NSP 4 proteins induced the highest IgG and IgA antibody levels. Thus, we concluded that the immunogenicity of a rotavirus DNA vaccine could be improved by co-expressing the VP 7 and NSP 4 proteins. This study was the first to construct a eukaryotic bicistronic plasmid DNA vaccine harboured by the prokaryotic expressing S. typhimurium χ 4550 strain and elicited desirable humoural and mucosal immune response against rotavirus. Further experiments are needed to elucidate the immunologic mechanism of this recombinant DNA vaccine Conclusion We described the construction of a bicistronic plasmid DNA vaccine against porcine rotavirus, which carries the NSP 4 and VP 7 genes simultaneously. BALB/c mice were orally immunised with an attenuated S. typhimurium χ 4550 strain expressing the vaccine. Significant levels of serum IgG, intestinal IgA and IFN- γ were induced by the reconstructed bicistronic DNA vaccine, suggesting that it may be a promising vaccine candidate against porcine rotavirus Acknowledgements We thank the Science and Technology support programme, Sichuan Province for integration and demonstration of the key technology research on modern pig industry Competing interests The authors declare that they have no financial or personal relationship(s) that may have inappropriately influenced them in writing this article Authors’ contributions S.C. (Sichuan Agricultural University) was the project leader. T.C. (Sichuan Agricultural University) and J.X. (Sichuan Agricultural University) were responsible for experiment and project design and performed most of the experiments. X.W. (Sichuan Agricultural University) and X.H. (Sichuan Agricultural University) made conceptual contributions, Y.W. (Sichuan Rural Science and Technology Development Centre) prepared the samples, Y.H. (Sichuan Agricultural University), X.M. (Sichuan Agricultural University) and Z.H. (Sichuan Agricultural University) performed some

Original Research doi:10.4102/ojvr.v 80 i 1.498 http://www.ojvr.org Page 8 of 8 of the experiments. Q.Z. (Sichuan Agricultural University) performed the calculations and guided the writing of the article. References Ball, J.M., Tian, P., Zeng, C.Q.Y, Morris, A.P. & Estes, M.K., 1996, ‘Age-dependent diarrhea induced by a rotaviral nonstructural glycoprotein’, Science 272, 101–104. http://dx.doi.org/10.1126/science.272.5258.101, PMid:8600515 Ball, J.M., Mitchell, D.M., Gibbons, T.F. & Parr, R.D., 2005, ‘Rotavirus NSP 4: A multifunctional viral enterotoxin’, Viral Immunology 18, 27–40. http://dx.doi org/10.1089/vim.2005.18.27, PMid:15802952 Buonaguro, F.M., Tornesello, M.L. & Buonaguro, L., 2009, ‘Virus-like particle vaccines and adjuvants: The HPV paradigm’, Expert Review of Vaccines 8, 1379–1398. http://dx.doi.org/10.1586/erv.09.81, PMid:19803760 Chen, S.C., Fynan, E.F., Robinson, H.L., Lu, S., Greenberg, H.B., Santoro, J.C. et al ., 1997, ‘Protective immunity induced by rotavirus DNA vaccines’, Vaccine 15, 899– 902. http://dx.doi.org/10.1016/S 0264-410 X(96)00272-1 Chen, S.C., Jones, D.H., Fynan, E.F., Farrar, G.H., Clegg, J.C.S, Greenberg, H.B. et al ., 1998, ‘Protective immunity induced by oral immunization with a rotavirus DNA vaccine encapsulated in microparticles’, Journal of Virology 72, 5757–5761. PMid:9621034 Coste, A., Sirard, J.C., Johansen, K., Cohen, J. & Kraehenbuhl, J.P., 2000, ‘Nasal immunization of mice with virus-like particles protects offspring against rotavirus diarrhea’, Journal of Virology 74, 8966–8971. http://dx.doi.org/10.1128/ JVI.74.19.8966-8971.2000, PMid:10982340 Estes, M.K., 2001, ‘Rotaviruses and their replication’, in D.M. Knipe, P.M. Howley, D.E. Griffin, R.A. Lamb, M.A. Martin, B. Roizman et al. (eds.), Fields Virology , 4 th edn., pp. 1747–1785, Lippincott Williams & Wilkins, Philadelphia Gebert, A., 1997, ‘The role of M cells in the protection of mucosal membranes’, Histochemistry and Cell Biology 108, 455–470. http://dx.doi.org/10.1007/ s 004180050186, PMid:9450628 Glass, R.I., Parashar, U.D., Bresee, J.S., Turcios, R., Fischer, T.K., Widdowson, M.A. et al ., 2006, ‘Rotavirus vaccines: Development, current prospects and future challenges’, Lancet 368, 323–32. http://dx.doi.org/10.1016/S 0140-6736(06)68815-6 Gonzalez, A.M., Nguyen, T.V., Azevedo, M.S.P., Jeong, K., Agarib, F., Iosef, C. et al ., 2004, ‘Antibody responses to human rotavirus (HRV) in gnotobiotic pigs following a new prime/boost vaccine strategy using oral attenuated HRV priming and intranasal VP 2/6 rotavirus-like particle (VLP) boosting with ISCOM’, Clinical & Experimental Immunology 135, 361–372. http://dx.doi.org/10.1111/j.1365- 2249.2004.02395.x, PMid:15008967 Herrmann, J.E., Chen, S.C., Jones, D.H., Tinsley-Bown, A., Fynan, E.F., Greenberg, H.B. et al ., 1999, ‘Immune responses and protection obtained by oral immunization with rotavirus VP 4 and VP 7 DNA vaccines encapsulated in microparticles’, Virology 259, 148–153. http://dx.doi.org/10.1006/viro.1999.9751, PMid:10364499 Hoshino, Y., Saif, L.J., Sereno, M.M., Chanock, R.M. & Kapikian, A.Z., 1988, ‘Infection immunity of piglets to either VP 3 or VP 7 outer capsid protein confers resistance to challenge with a virulent rotavirus bearing the corresponding antigen’, Journal of Virology 62, 744–748. PMid:2828669 Kavanagh, O.V., Ajami, N.A., Cheng, E., Ciarlet, M., Guerrero, R.A., Zeng, C.Q.Y. et al ., 2010, ‘Rotavirus enterotoxin NSP 4 has mucosal adjuvant properties’, Vaccine 28, 3106–3111. http://dx.doi.org/10.1016/j.vaccine.2010.02.063, PMid:20197138 Lintermans, P. & Greve, H.D., 1995, ‘Live bacterial vectors for mucosal immunization’, Advanced Drug Delivery Reviews 18, 73–89. http://dx.doi.org/10.1016/0169- 409 X(95)00051-8 Matsui, S.M., Offit, P.A., Vo, P.T., Mackow, E.R., Benfield, D.A., Shaw, R.D. et al ., 1989, ‘Passive protection against rotavirus-induced diarrhea by monoclonal antibodies to the heterotypic neutralization domain of VP 7 and the VP 8 fragment of VP 4’, Journal of Clinical Microbiology 27, 780–782. PMid:2470774 O’Neal, C.M., Crawford, S.E., Estes, M.K. & Conner, M.E., 1997, ‘Rotavirus virus-like particles administered mucosally induce protective immunity’, Journal of Virology 71, 8707–8717. PMid:9343229 Pasetti, M.F., Levine, M.M. & Sztein, M.B., 2003, ‘Animal models paving the way for clinical trials of attenuated Salmonella enterica serovar typhi live oral vaccines and live vectors’, Vaccine 21, 401–418. http://dx.doi.org/10.1016/S 0264- 410 X(02)00472-3 Petrie, B.L., Estes, M.K. & Graham, D.Y., 1983, ‘Effects of tunicamycin on rotavirus morphogenesis and infectivity’, Journal of Virology 46, 270–274. PMid:6298460 Ribes, J.M., Ortego, J., Ceriani, J., Montava, R., Enjuanes, L. & Buesa, J., 2011, ‘Transmissible gastroenteritis virus (TGEV)-based vectors with engineered murine tropism express the rotavirus VP 7 protein and immunize mice against rotavirus’, Virology 410, 107–118. http://dx.doi.org/10.1016/j.virol.2010.10.036, PMid:21094967 Schoen, C., Stritzker, J., Goebel, W. & Pilgrim, S., 2004, ‘Bacteria as DNA vaccine carriers for genetic immunization’, International Journal of Medical Microbiology 294, 319–335. http://dx.doi.org/10.1016/j.ijmm.2004.03.001, PMid:15532991 Song, X.F. & Hao, Y., 2009, ‘Adaptive evolution of rotavirus VP 7 and NSP 4 genes in different species’, Computational Biology and Chemistry 33, 344–349. http:// dx.doi.org/10.1016/j.compbiolchem.2009.07.008, PMid:19665933 Ward, L.A., Rich, E.D. & Besser, T.E., 1996, ‘Role of maternally derived circulating antibodies in protection of neonatal swine against porcine group A rotavirus’, Journal of Infectious Diseases 174, 276–282. http://dx.doi.org/10.1093/ infdis/174.2.276, PMid:8699055 Ward, R.L., Knowlton, D.R., Greenberg, H.B., Schiff, G.M. & Bernstein, D.I., 1990, ‘Serum-neutralizing antibody to VP 4 and VP 7 proteins in infants following vaccination with WC 3 bovine rotavirus’, Journal of Virology 64, 2687–2691. PMid:2159538 Ward, R.L., McNeal, M.M., Sander, D.S., Greenberg, H.B. & Bernstein, D.I., 1993, ‘Immunodominance of the VP 4 neutralization protein of rotavirus in protective natural infections of young children’, Journal of Virology 67, 464–468. PMid:8416378 Yang, H., Cao, S.J., Huang, X.B., Liu, J.W., Tang, Y. & Wen, X.T., 2009, ‘Intragastric administration of attenuated Salmonella typhimurium harbouring transmissible gastroenteritis virus (TGEV) DNA vaccine induced specific antibody production’, Vaccine 27, 5035–5340. http://dx.doi.org/10.1016/j.vaccine.2009.06.050, PMid:19573642 Yang, K.J., Wang, S.X., Chang, K.O., Lu, S., Saif, L.J., Greenberg, H.B. et al ., 2001, ‘Immune responses and protection obtained with rotavirus VP 6 DNA vaccines given by intramuscular injection’, Vaccine 19, 3285–3291. http://dx.doi org/10.1016/S 0264-410 X(00)00543-0 Yuan, L., Iosef, C., Azevedo, M.S.P., Kim, Y., Qian, Y., Geyer, A. et al ., 2001, ‘Protective immunity and antibody-secreting cell responses elicited by combined oral attenuated Wa human rotavirus and intranasal Wa 2/6-VLPs with mutant Escherichia coli heat-labile toxin in gnotobiotic pigs’, Journal of Virology 75, 9229– 9238. http://dx.doi.org/10.1128/JVI.75.19.9229-9238.2001, PMid:11533185

Other Health Sciences Concepts:

[back to top]

Discover the significance of concepts within the article: ‘Construction of an artificial recombinant bicistronic plasmid DNA vaccine...’. Further sources in the context of Health Sciences might help you critically compare this page with similair documents:

Cell, Gene, Strain, Elisa, Diarrhoea, Immune system, Mucosal membrane, Gene expression, Immune response, RT-PCR, Vaccine, IgG antibody, Gastroenteritis, Cellular immunity, S. Typhimurium, Oral delivery, Salmonella typhimurium, Mucosal immune response, Monoclonal antibodies, Serum IgG, IgG antibodies, Oral immunization, Antibody Response, Antigen, Mucosal surface, Safety analysis, Rotavirus vaccine, Neutralizing antibody, Expression vector, Immunofluorescence assay, Vaccine candidate, Viral strain, Indirect ELISA, DNA vaccine, Adjuvant properties, Mucosal immunity, Rotavirus infection, Intussusception, Neutralising antibodies, Humoural immune response, Protective immunity, Bacterial colony, Recombinant plasmid, Host cell, Plasmid, Reverse transcription-polymerase chain reaction, IgA antibody, Gene segment, Vector, Gene fragment, Viral gene, IgA antibodies, Live bacteria, Genetic structure, COS-7 cells, Eukaryotic expression vector, Structural protein, Viral challenge, Vaccine vectors, IgG antibody response, Sichuan Agricultural University, DNA vaccine carriers.