Isolation and antioxidant activity of 5-methyldihydroflavasperone from...

[Full title: Isolation and antioxidant activity of 5-methyldihydroflavasperone from ethanol leaf extract of Guiera senegalensis JF Gmel]

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http://www.jomped.org Open Access Journal of Medicinal Plants for Economic Development ISSN: (Online) 2616-4809, (Print) 2519-559 X Page 1 of 7 Original Research Read online: Scan this QR code with your smart phone or mobile device to read online Authors: Mosa E.O. Ahmed 1 Jeremiah Senabe 2 Ewura S. Yahaya 3 Gerda Fouche 4 Paul Steenkamp 5 Vanessa Steenkamp 6 Affiliations: 1 Department of Pharmacognosy, Faculty of Pharmacy, Alneelain University, Khartoum, Sudan 2 Council for Scientific and Industrial Research, Pretoria, South Africa 3 Department of Pharmacology, School of Medical Sciences, University of Cape Coast, Cape Coast, Ghana 4 Department of Chemistry, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria, South Africa 5 Research Centre for Plant Metabolomics, Department of Biochemistry, University of Johannesburg, Johannesburg, South Africa 6 Department of Pharmacology, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa Corresponding author: Mosa Ahmed, alhalim 21@yahoo.com Dates: Received: 28 July 2021 Accepted: 27 Nov. 2021 Published: 28 Feb. 2022 Introduction Traditional medicine remains an important aspect of health care globally, and is widely used in the treatment and prevention of diseases (Li & Weng 2017). Furthermore, it serves as a promising source of phytochemical compounds used for the development of conventional medications (Yuan et al. 2016). In Africa and other developing countries, most people rely on herbal medications for primary healthcare (Malami et al. 2020). Guiera senegalensis (Combretaceae) is a medicinal plant that is widely distributed in the savannah regions of west and central African countries, such as Nigeria, Senegal, Gambia, Mali, Niger, Burkina Faso and Ghana (Salihu & Usman 2015). In Sudan, where it is locally known as ‘ Ghibaish’ , the leaves are used for leprosy prevention, whilst a root decoction is used for the treatment of diarrhoea and dysentery (Mariod, Matthäus & Hussein 2006). A herbal tea prepared from the plant is used to treat malaria, severe diarrhoea, dysentery, eczema, chest conditions and cold (Ifijen et al. 2019; Somboro et al. 2011). Several compounds have been isolated from the leaves of G. senegalensis ; the naphthopyran, 5-methylflavasperone (5,8,10-trimethoxy-2-methyl-4-H-naphtho[1,2-b]pyran-4-one); rhamnetin from the methylene chloride extract (Bucar et al . 1998) and 5-methyldihydroflavasperone (2,3-dihydro-5,8,10-trimethoxy-2-methyl-4 H-naphtho[1,2-b]pyran-4-one) (Figure 1) from the chloroform extract (Mahmoud & Khalid 1997). Both 5-methylflavasperone and rhamnetin are reported to induce inhibitory effects on 5-lipoxygenase and the hydroxyl radical (Bucar et al . 1998). Other identified compounds include guieranone A, an antifungal compound, and the indole alkaloids: harman, harmalan and tetrahydroharman (Fiot et al. 2006; Silva & Gomes 2003) Background: Guiera senegalensis ( Ghibaish ) is a medicinal plant extensively used in central and west Africa for the management of various diseases. Aim: This study aimed to determine the antioxidant activity of the ethanol leaf extract of the plant Method: The ethanol leaf extract was sequentially sub-fractionated using liquid–liquid extraction, vacuum-liquid chromatography and preparative thin layer chromatography. Ultraperformance liquid chromatography with accurate mass spectrometry and nuclear magnetic resonance were employed to isolate and confirm the identity of the most abundant compound. The antioxidant activity of the fractions and isolated compound was assessed by bioautography analysis and the 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) radical scavenging assay. Results: The dichloromethane subfraction contained the most promising antioxidant activity (IC 50 = 3.18 µg/mL). Purification of this subfraction led to the isolation of a brown crystalline compound, which was identified as 5-methyldihydroflavasperone (IC 50 > 4000 µg/mL). Conclusion: This is the first report of the isolation of 5-methyldihydroflavasperone from the ethanol leaf extracts of G. senegalensis . This compound was not found to be responsible for the antioxidant activity observed. Further research is warranted to identify the compound responsible for the antioxidant activity. Keywords: Antioxidant activity; DPPH assay; Guiera senegalensis ; 5-methyldihydroflavasperone; NMR spectroscopy; UPLC-MS analysis Isolation and antioxidant activity of 5-methyldihydroflavasperone from ethanol leaf extract of Guiera senegalensis JF Gmel Read online: Scan this QR code with your smart phone or mobile device to read online How to cite this article: Ahmed, M.E.O., Senabe, J., Yahaya, E.S., Fouche, G., Steenkamp, P. & Steenkamp, V., 2022, ‘Isolation and antioxidant activity of 5-methyldihydroflavasperone from ethanol leaf extract of Guiera senegalensis JF Gmel’, Journal of Medicinal Plants for Economic Development 6(1), a 137. https://doi.org/10.4102/jomped.v 6 i 1.137 Copyright: © 2022. The Authors. Licensee: AOSIS. This work is licensed under the Creative Commons Attribution License.

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Page 2 of 7 Original Research http://www.jomped.org Open Access To add to the knowledge pool on G. senegalensis , this study aimed to determine the antioxidant activity of the ethanol leaf extract of the plant Materials and methods Chemicals and solvents All chemicals, solvents and reagents used are of analytical grade. The 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) radical reagent was purchased from Sigma-Aldrich (Germany). Ascorbic acid was purchased from Merck chemicals (PTY) Ltd (SA). Aluminium-coated plates (silica gel 60 F 254; 0.25 mm, 20 cm × 20 cm) were purchased from Merck (Germany) and preparative silica gel plates (silica gel 60 F 254; 2 mm, 20 cm × 20 cm) from Anatech (USA). All the solvents used for mass spectrometric analysis were of the highest purity and procured from Honeywell (Muskegon, USA) Sample collection and preparation Guiera senegalensis J.F. Gmel. leaves were collected from Northern Kurdufan state in Sudan in October 2015. The specimen was authenticated by Dr Yahiya Suleiman of the Medicinal and Aromatic Plants Research Institute, Sudan (MAPRI) where a voucher specimen (W-1997-21-MAPTMRI-H) is deposited Sample preparation Collected leaves (2 kg) were dried in the shade, after which they were macerated and extracted twice with 96 % ethanol (10.35 L) for 72 h. The obtained extract was filtered under gravity and concentrated using a rotary evaporator (Laborota 4000-Efficient, Heidolph, Germany). The filtrate (135.9 g) was dried in vacuo , resulting in a solid marc, which was green or black in colour. The latter was stored in an airtight bottle at 2° C until analyses were carried out. Sample extraction Fractionation was achieved by dissolving the ethanol extract (135.9 g) in chloroform (750 mL) and mixing with distilled water (450 mL). The chloroform fraction was partitioned between hexane (1.85 L) and 90 % methanol:water (700 mL). The resultant fraction was diluted with distilled water (200 mL) and extracted with dichloromethane (900 mL) (Ayoub & Kingston 1984). All the fractions were evaporated, air-dried and kept in sealed containers, which were stored at 2°C until use (Figure 2) All subfractions were monitored for antioxidant activity in order to identify the most active subfraction, which was subjected to further purification (as described in the following section) Purification of subfraction with highest activity The dichloromethane soluble fraction (56.95 g) was further purified by vacuum-liquid chromatography with silica gel as the stationary phase. An aliquot (346 g) was loaded onto the silica gel column and sequentially eluted with hexane (6 L), hexane: ethyl acetate (4:6; 3.5 L), dichloromethane (1.7 L), acetone (3 L), ethyl acetate (1.25 L) and methanol (4.65 L) The dichloromethane subfraction (0.45 g) was subjected to further purification on preparative thin layer chromatography (TLC) and visualisation occurred as described here. Ten bands were detected. Band number 4 ( R f = 0.41, yellow or green colour under normal light, green colour under ultraviolet [UV] light) was removed by scraping and dissolved in dichloromethane. The new subfraction was filtered and then loaded onto a preparative TLC plate (3 mL). The mobile phase used for development consisted of toluene:ethyl acetate:formic acid (6:3.5:0.5). This afforded the detection of 15 bands when visualised using both normal and UV light. Of the 15 bands, the seventh band ( R f = 0.59, deep yellow colour) was removed by scraping it off, after which it was dissolved in dichloromethane. The fraction was filtered and eluted with toluene:ethyl acetate:formic acid (3:6.9:0.1). After subjection to OMe 7 8 9 10 1 2 3 4 4 a 5 6 6 a 10 a 10 b O OMe MeO O FIGURE 1: Chemical structure of 5-methyldihydroflavasperone H 2 O Soluble fraction CHCI 3 Soluble fraction Hex/MeOH fraction DCM fraction Hex subfraction Hex/EtOAc subfraction DCM subfraction AcOEt subfraction EtOAc subfraction MeOH subfraction Hex/MeOH H 2 O/DCM Hex Hex/EtOAc DCM AcOEt EtOAc MeOH Liquid-Liquid Fractionation Vacuum-Liquid Chromatography Plant sample EtOH EtOH extract CHCI 3 /H 2 O FIGURE 2: Schematic presentation summarising the extract preparation and purification of the fractions. AcOEt, acetone; DCM, dichloromethane; EtOAc, ethyl acetate; EtOH, ethanol; CHCl 3 , chloroform; H 2 O, water; Hex, hexane; MeOH, methanol.

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Page 3 of 7 Original Research http://www.jomped.org Open Access elution using TLC (silica gel GF, 2 mm) and visualisation using normal and UV light, 13 bands were obtained. From the latter, the 5 th band ( R f = 0.62, deep yellow colour) was scraped off and dissolved in dichloromethane, filtered and dried. This yielded brown crystals, which were subjected to further analysis to determine compound identity Structure elucidation of the isolated compound Structure elucidation of the compound was carried out using 1 H-NMR and 13 C-NMR on a 600 MHz Varian NMR instrument (Varian Inc., USA) The molecular mass was determined using a Waters Acquity Classic UPLC System coupled to a Waters Synapt G 1 HDMS mass spectrometer (Waters, UK). Mass spectrometric (MS) analysis was carried out in full-scan mode in both ionisation modes. A Waters Classic binary Ultra-performance liquid chromatography (UPLC) system, coupled in series to a Waters SYNAPT G 1 HDMS mass spectrometer was used to generate full scan and MS/MS accurate mass data. Optimisation of the chromatographic separation was carried out utilising a Waters HSS T 3 C 18 column (150 mm × 2.1 mm, 1.8 µm) and the column temperature was maintained at 60°C. A binary solvent mixture was used consisting of water (Eluent A), which contained 10 mM formic acid (natural pH of 2.4) and acetonitrile (Eluent B) containing 10 mM formic acid. The initial conditions were 80 % A at a flow rate of 0.4 mL/min and were maintained for 1 min, followed by a gradient increase to 5 % A at 16 min. The conditions were kept constant for 1 min and then changed to the initial conditions. The total runtime was 20 min and the injection volume was 2 µL. Samples were maintained at 6°C in the Waters Sample Manager during analysis A SYNAPT G 1 mass spectrometer was used in V-optics configuration and operated in electrospray mode to enable detection of all electrospray ionisation (ESI)-compatible compounds. Leucine enkephalin (50 pg/mL) was used as a reference calibrant (Lock Mass) to obtain typical mass accuracies between 1 mDa and 5 mDa. The mass spectrometer was operated in both ESI-positive and -negative modes, with a capillary voltage of 2.5 kV, the sampling cone at 30 V and the extraction cone at 4.5 V. The scan time was 0.1 s covering the 50 Da to 1000 Da mass range with an interscan time of 0.02 s. The source temperature was set at 120°C and the desolvation temperature at 450°C. Nitrogen was used as the nebulisation gas at a flow rate of 550 L/h, and cone gas was added at 50 L/h. Argon was used as collision gas in the collision cell during fragmentation experiments. The software used to control the hyphenated system and do all data manipulation was MassLynx 4.1 (SCN 872). Compound identification was further enhanced by analysing all samples with low and high collision energy settings of the collision cell. To minimise compound fragmentation a low-energy setting of 3 V was used, but to enhance fragmentation of molecules, five different collision energy profiles between 10 V and 50 V were used (MS e ) Antioxidant activity Bioautography The subfractions were dissolved in the respective solvent of extraction and loaded as spots onto the TLC plates (silica gel 60 F 254 , 0.25 mm thickness). The plates were placed in a saturated development chamber with a mobile phase consisting of toluene: ethyl acetate: formic acid (5:4:1). The plates were removed and air-dried when the solvent front was 1 cm from the top After development, plates were sprayed with vanillin sulphuric acid and visualised under normal and UV light (254 nm and 365 nm, respectively) (Model NU-8 KL, Benda, Germany). The presence of any fluorescent or quenching compounds was observed as white or yellow spots against a purple background (Gu, Wu & Wang 2009). 2,2-diphenyl-1-picrylhydrazyl hydrate radical radical scavenging assay The DPPH radical scavenging activity was determined using the method described by Brand-Williams, Cuvelier and Berset (1995) with minor modification. The reduction of DPPH by the antioxidant compound results in a change in colour from deep violet to yellow. An aliquot of 100 µL DPPH (0.135 mM in methanol) was added to varying concentrations of the extracts (3 µg/mL–100 µg/mL; 100 µL) or the positive control, ascorbic acid (1 mM) in a 96-well plate. The mixture was incubated in the dark for 30 min at room temperature, and the absorbance was measured using a spectrophotometer (BioTek Synergy 2, BioTek instruments, USA) set at an absorbance wavelength of 515 nm. The ability of extracts to scavenge the DPPH radical was calculated using the following equation: DPPH radical scavenging activity % relative to negative control = A A control A sample A control 100 [Eqn 1] where A control is the absorbance of DPPH radical + methanol and A sample is the absorbance of DPPH radical + extract or standard. The IC 50 for each extract was calculated using computer software (Graph Pad prism 7, Graphpad Software Inc., USA). The assays were conducted in triplicate on three different occasions Ethical considerations This study followed all ethical standards for research without direct contact with human or animal subjects Results and discussion Fractionation and purification of the extract Fractionation of the ethanol leaf extract using liquid–liquid extraction resulted in four fractions: water soluble fraction (gummy brown; yield 23.50 % ), hexane soluble fraction (oily/green; yield 5.61 % ), methanol:water (8:1) soluble fraction (gummy/brown; yield 14.40 % ) and dichloromethane

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Page 4 of 7 Original Research http://www.jomped.org Open Access soluble fraction (amorphous/green; yield 45.10 % ). As the dichloromethane fraction had the highest yield, it was subjected to further purification using a vacuum column resulting in six subfractions. As shown in Figure 3, the six subfractions of the dichloromethane fraction were spotted onto a TLC plate (silica gel 60 F 254 , 0.25 mm thickness) for bioautography analysis. These chromatograms were visualised under normal light (plate A) and UV 254 light (plate B). All subfractions indicated the same number of spots when visualised using either light options. The sixth subfraction (methanol) was the only subfraction that was devoid of yellow florescent spots when visualised under UV 254 light Structure elucidation of isolated compound 1 Hand 13 C-NMR analysis The 1 H-NMR spectrum showed a doublet at δ 1.57 indicative of a methyl group, a multiplet at δ 2.69 indicative of a methylene group, and three singlets at δ 3.94, 3.88 and 3.89 related to three methoxyl groups at C-5, C-8 and C-10, respectively, with the multiplet at δ 4.64 attributed to one proton (Table 1 and Figure 4). The aromatic region showed two doublets at δ 6.32 and 6.52 and a singlet at δ 6.54. This finding is consistent with that of an earlier study on the chloroform extract of the plant where 5-methyl-dihydroflavasperone (2,3-dihydro-5,8,10-trimethoxy- 2-methyl-4 H-naphtho [1,2-b] pyran-4-one) was identified (El Hadi & Khalid 1997) The 13 C-NMR spectral data indicated 17 carbon atoms and showed a chemical shift at δ 191.0, which is indicative of a carbonyl carbon (Table 2 and Figure 5). 13 C-NMR analysis also indicated the presence of a methyl group ( δ 20.9), three methoxyl groups ( δ 56.0, 55.6 and 56.2), one methylene ( δ 45.6) and one ethyl group at δ 74.9. Three aromatic carbons were also observed at δ 98.6, 97.5 (ring b) and 99.3 (ring a). A similar finding has been reported for 5-methyldihydroflavasperone (El Hadi & Khalid 1997) TABLE 2: Comparison between 13 C-NMR spectral data (600 MHz, CDCl 3 ) of the subfraction (4/7/5) and 5-methyl-dihdroflavasperone Assignment Position (ppm) Position (ppm) (El Hadi & Khalid 1997) 2-CH 74.9 74.6 2-CH 3 20.9 20.6 3-CH 2 45.6 45.3 4-C=O 191.0 190.6 4 a-C=C- 108.5 108.1 5-C=C- 157.9 157.6 5-OCH 3 56.0 55.7 6-C=C- 99.3 98.9 6 a-C=C- 141.7 141.4 7-C=C- 98.6 98.4 8-C=C- 161.8 161.5 8-OCH 3 55.6 55.2 9-C=C- 97.5 97.2 10-C=C- 160.4 160.1 10-OCH 3 56.2 55.8 10 a-C=C- 107.6 107.3 10 b-C=C- 164.3 164.0 TABLE 1: 1 H-NMR spectral data (600 MHz, CDCl 3 ) of the subfraction (4/7/5) and 5-methyldihdroflavasperone Assignment Position (ppm) Position (ppm) (El Hadi & Khalid 1997) Integration Multiplicity 2-CH- 4.64 4.7 (m) 1 H Multiplet 2-CH 3 1.57 1.6 (d) 3 H Doublet 3-CH 2 2.69 2.7 (m) 2 H Multiplet 5-OCH 3 3.94 3.96 (s) 3 H Singlet 6-CH 6.54 6.52 (s) 1 H Singlet 7-CH 6.52 6.49 (d) 1 H Doublet 8-OCH 3 3.88 3.9 (s) 3 H Singlet 9-CH 6.32 6.3 (d) 1 H Doublet 10-OCH 3 3.89 3.92 (s) 3 H Singlet a b FIGURE 3: Bioautography analysis of subfractions of the dichloromethane fraction using (a) normal light and (b) UV light Lane 1: hexane (oily/yellow), lane 2: ethyl acetate:hexane (4:6) (oily/green), lane 3: dichloromethane (solid/ brown), lane 4: acetone (solid/green), lane 5: ethyl acetate (solid/green), lane 6: methanol (solid/brown).

Page 5 of 7 Original Research http://www.jomped.org Open Access 6.536 7 6 5 4 3 2 1 ppm 6.522 6.51 9 6.32 1 6.31 7 4.65 4 4.64 4 4.53 9 3.937 3.983 3.882 2.75 2 2.73 3 2.72 5 2.70 3 2.67 5 2.64 9 2.64 7 2.64 2 1.56 5 1.581 1.570 1.24 6 FIGURE 4: 1 1 H-NMR spectral data (600 MHz, CDCl 3) of sub-fractions of the dichloromethane fraction of G. senegalensis showing a doublet at δ 1.57, a multiplet at δ 2.69, three singlets at δ 3.94 - 3.89, and a multiplet at δ 4.64 20.888 45.640 55.551 56.057 56.226 74.952 77.014 77.436 97.466 99.253 107.616 108.473 141.733 157.907 160.375 161.067 164.331 191.029 200 180 160 140 120 100 80 60 40 20 ppm 98.030 77.029 FIGURE 5: 13 C-NMR spectral data (600 MHz, CDCl 3) of sub-fractions of the dichloromethane fraction of G. senegalensis showing 17 carbons.

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Page 6 of 7 Original Research http://www.jomped.org Open Access UPLC-MS analysis The molecular mass was determined using a Waters Acquity Classic UPLC System coupled to a Waters Synapt G 1 HDMS mass spectrometer (Waters, UK). MS analysis was carried out in full-scan mode in both ionisation modes. The leading compound was detected in positive ionisation mode with precursor [M+H] + m/z 303.1237 and elemental composition C 17 H 19 O 5 and a double bond equivalent (DBE) count of 9. The theoretical calculation of C 17 H 18 O 5 , m/z 302.1154 indicated a mass accuracy of 0.5 mDa, and the isotope ratio was 0.00 (perfect score) MS e analysis resulted in five product ions, namely [M+H-CH 3 ] + , m/z 288.0990; [M+H-C 2 H 6] + , m/z 273.0760; [M+H-C 3 H 9 ] + , m/z 258.0518; [M+H-C 4 H 8 ] + , m/z 247.0604 and [M+H-C 5 H 9 O] + , m/z 218.0564. The results were confirmed by MassFragment analysis. MassFragment software automatically identifies product ion fragments using a series of novel, chemically intelligent algorithms. This approach is based on systematic bond disconnection of the precursor structure instead of the traditional rule-based approach, which is limited to the extent that the rules are coded and will not always provide the information required The MS spectral data of the subfraction (4/7/5) are provided in Figure 6. Elemental analysis of the compound was calculated as C 17 H 18 O 5 , which corresponds to an earlier study on the chloroform extract of the plant (El Hadi & Khalid 1997) Antioxidant activity The dichloromethane subfraction exhibited the highest antioxidant activity (3.18 µg/mL) (Table 3), which was 30 % higher than that of the standard antioxidant, ascorbic acid. Previous studies have reported the antioxidant activity of extracts prepared from various parts of the plant. The antioxidant potential of aqueous extracts prepared from the roots, stems and leaves is known (Atawodi & Onaolapo 2010). Other studies on the ethanol and methanol extracts of the plant also reported similar findings. As reported by Adebayo et al. (2019), the IC 50 of methanol extract for the bark of G. senegalensis was 19.07 µg/mL, whereas Parvez et al. (2018) reported that the IC 50 of ethanol extract for leaves of G. senegalensis was 82.7 µg/mL. These results were higher than that obtained in this study (10.21 µg/mL) Tannins isolated from the galls of G. senegalensis include gallic acid, epicatechin and galloylquinic acids, all of which have been shown to possess antioxidant activity (Bouchet, Barrier & Fauconneau 1998). More recently, the presence of four antioxidant biomarkers: β -amyrin, β -sitosterol, lupeol and ursolic acid, was reported in the leaves of G. senegalensis (Parvez et al. 2018). Although 5-methyldihydroflavasperone has been Mosa DCM_4 02 Nov % 0 1.00 5.45 303.0894 8.86 317.1002 9.98 303.1172 5-MethyIdihydroflavasperone WATERS SYNAPTG 1 15.05 628.4644 1: TOF MS ES+ BPI 7.28 e 4 2.00 3.00 4.00 5.00 6.00 7.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 18.00 19.0 Time 17.00 8.00 FIGURE 6: Ultra-performance liquid chromatography Time-of-Flight positive electrospray base peak intensity (UPLC TOF ESI + BPI) chromatogram of the subfraction (4/7/5) with the highest antioxidant activity TABLE 3: The IC 50 of ethanol extract, dichloromethane fraction, dichloromethane subfractions and ascorbic acid (standard) as determined using the 2,2-diphenyl- 1-picrylhydrazyl hydrate radical assay Extracts/fractions/sub-fractions IC 50 (µg/mL) 96 % ethanol extract 10.21 ± 0.07 Dichloromethane soluble fraction 4.73 ± 0.10 Hexane subfraction > 150 40 % ethyl acetate: hexane subfraction > 150 Dichloromethane subfraction 3.18 ± 0.06 Acetone subfraction 7.19 ± 0.07 Ethyl acetate subfraction 16.42 ± 0.08 Methanol subfraction 5.54 ± 0.06 5-Methyldihydroflavasperone > 4000 Standard Ascorbic acid 4.65 ± 0.23

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Page 7 of 7 Original Research http://www.jomped.org Open Access extracted from the chloroform leaf extract of the plant (El Hadi & Khalid 1997), the authors are not aware of any determination of antioxidant activity of the compound. In this study, the compound was isolated from the dichloromethane subfraction of the ethanol extract of the leaves. A very weak antioxidant potential was observed when compared with the antioxidant standard and the subfractions (4/7/5). This suggests that the antioxidant activity observed is rather ascribed to other compounds and not to 5-methyldihydroflavasperone. Conclusion This is the first report on the isolation of 5-methyldihydroflavasperone from the ethanol leaf extracts of G. senegalensis . This compound was not found to be responsible for the observed antioxidant activity. Further research is warranted to assess the compound responsible for the antioxidant activity Acknowledgements The authors extend their sincere gratitude to the Pretoria University and CSIR, South Africa and Alneelain University, Sudan for their support, guidance and Sudan Ministry of Higher Education for provision of research visit to the first author. Also, the authors thanks Dr Yahiya Suleiman of the Medicinal and Aromatic Plants Research Institute, Sudan (MAPRI) for plant sample authentication Competing interests The authors declare that they have no financial or personal relationships that may have inappropriately influenced them in writing this article Authors’ contributions M.E.O.A. and J.S. carried out the extraction and purification of plant samples. M.E.O.A. and E.S.Y. performed data collection, carried out the antioxidant testing of plant extracts, fractions and isolates, and writing the draft of this article. M.E.O.A. and G.F. designed the NMR method and analysed NMR spectroscopy results. M.E.O.A. and P.S. designed, performed and analysed UPLC-MS analysis results. V.S. verified the methodology and supervised the findings of this work. All authors discussed the results and contributed to the final manuscript Funding information M.E.O.A. received funding from the Sudan Ministry of Higher Education in the form of a scholarship to undertake this study Data availability Data will be made available upon request from the corresponding author Disclaimer The views and opinions expressed in this article are those of the authors and do not necessarily reflect the official policy or position of any affiliated agency of the authors References Adebayo, I.A., Gagman, H.A., Balogun, W.G., Adam, M.A.A., Abas, R., Hakeem, K.R. et al., 2019, ‘ Detarium microcarpum, Guiera senegalensis , and Cassia siamea induce apoptosis and cell cycle arrest and inhibit metastasis on MCF 7 breast cancer cells’, Evidence-Based Complementary and Alternative Medicine 23, 6104574. https://doi.org/10.1155/2019/6104574 Atawodi, S.E. & Onaolapo, G.S., 2010, ‘Comparative in vitro antioxidant potential of different parts of Ipomoea asarifolia , Roemer & Schultes, Guiera senegalensis , JF Gmel and Anisopus mannii NE Brown’, Brazilian Journal of Pharmaceutical Sciences 46(2), 245–250. https://doi.org/10.1590/S 1984- 82502010000200011 Ayoub, S.M. & Kingston, G.I., 1984, ‘Lariciresinol derivatives from Turrea nilotica and Monechma ciliatum ’, Journal of Natural Products 47(5), 875–876. https://doi. org/10.1021/np 50035 a 023 Bouchet, N., Barrier, L. & Fauconneau, B., 1998, ‘Radical scavenging activity and antioxidant properties of tannins from Guiera senegalensis (Combretaceae)’, Phytotherapy Research 12(3), 159–162. https://doi.org/10.1002/(SICI)1099- 1573(199805)12:3<159::AID-PTR 209>3.0.CO;2-C Brand-Williams, W., Cuvelier, M.-E. & Berset, C., 1995, ‘Use of a free radical method to evaluate antioxidant activity’, Food Science and Technology 28(1), 25–30. https:// doi.org/10.1016/S 0023-6438(95)80008-5 Bucar, F., Resch, M., Bauer, R., Burits, M., Knauder, E. & Schubert-Zsilavecz, M., 1998, ‘5-Methylflavasperone and rhamnetin from Guiera 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