Cytomorphometric study of gingival cells in healthy vs. periodontitis.

| Posted in: Science

Journal name: Journal of Indian Society of Periodontology
Original article title: Comparative assessment of exfoliated gingival cells in healthy individuals and chronic periodontitis patients with and without tobacco chewing habit: A cytomorphometric study
The Journal of Indian Society of Periodontology (JISP) publishes original scientific articles on periodontology (the study of supporting structures of teeth) and oral implantology. It is a bimonthly open-access journal with special issues for specific occasions.
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Original source:

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Author:

Sachita S. Naik, Aarati Nayak, Vijaylakshmi Kottrashetti, Akanksha Bhatt


Journal of Indian Society of Periodontology:

(A bimonthly open-access journal)

Full text available for: Comparative assessment of exfoliated gingival cells in healthy individuals and chronic periodontitis patients with and without tobacco chewing habit: A cytomorphometric study

Year: 2022 | Doi: 10.4103/jisp.jisp_817

Copyright (license): CC BY-NC-SA


Summary of article contents:

Introduction

Cytomorphometry, the study of the morphology of exfoliated cells, has not been extensively applied to exfoliated gingival epithelial cells in chronic periodontitis, a condition often exacerbated by tobacco use. This study aimed to examine and compare the nuclear area (NA), cytoplasm area (CA), and nuclear: cytoplasm ratio (N/C) in gingival cells from healthy individuals, patients with chronic periodontitis, and those with chronic periodontitis coupled with a history of tobacco chewing. Through the analysis of 90 subjects aged 35-45, categorized into three equal groups, the study utilized Papanicolaou staining and advanced image analysis software, thereby seeking to uncover any cytomorphometric variations associated with the conditions studied.

Cytomorphometric Changes in Chronic Periodontitis

The results highlighted a statistically significant increase in cytoplasm area across all groups, indicating cellular changes associated with periodontitis and tobacco use. While NA and N/C ratio did not show significant differences between groups, correlations emerged between CA and clinical parameters like probing depth and clinical attachment loss (CAL). Specifically, the chronic periodontitis group demonstrated significant correlations of CA with probing depth and CAL, whereas the tobacco chewing group also displayed similar trends. These findings point to the presence of cellular-level changes indicative of underlying pathologies, even in the absence of apparent clinical signs, establishing cytomorphometry as a valuable investigative tool.

Conclusion

The study concludes that noninvasive cytological assessments can reveal significant alterations in cytomorphometric indices, which may precede detectable clinical changes in chronic periodontitis. By employing cytomorphometric analysis on exfoliated gingival cells, practitioners can potentially predict periodontal disease progression and provide timely interventions. The results underscore the necessity for further research into cellular changes across various stages of periodontitis to enhance diagnostic accuracy and inform treatment strategies.

FAQ section (important questions/answers):

What is the objective of the cytomorphometric study?

The study aims to correlate nuclear area, cytoplasm area, and nuclear: cytoplasm ratio in gingival cells of healthy individuals and chronic periodontitis patients, including those who chew tobacco.

How were the subjects classified in the study?

Ninety subjects aged 35–45 years were divided into three groups: healthy individuals, chronic periodontitis patients without harmful habits, and chronic periodontitis patients who are tobacco chewers.

What significant findings were reported in the study?

The study found a significant increase in cytoplasm area in all groups, while nuclear area and nuclear: cytoplasm ratio showed no significant differences.

How does cytomorphometry help in periodontal disease?

Cytomorphometry can analyze cellular changes in chronic periodontitis patients at a molecular level, potentially detecting alterations before clinical signs become evident.

What method was used for collecting samples in the study?

Samples were collected using a medium nylon toothbrush to procure exfoliated gingival cells from the subjects, which were then analyzed microscopically after staining.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Cytomorphometric study of gingival cells in healthy vs. periodontitis.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Table:
The 'Table' is a structured arrangement used to display data orderly for analysis and interpretation within the study. It helps summarize findings such as nuclear area, cytoplasm area, and nuclear-cytoplasm ratio values. Tables are crucial for conveying complex information concisely and are used to support results and facilitate comparisons across different groups in research.

2) Study (Studying):
The 'Study' refers to the systematic investigation undertaken to understand a particular aspect of chronic periodontitis and cytomorphometric changes in gingival cells. It aims to gather data, test hypotheses, and draw conclusions about healthy individuals versus those affected by chronic periodontitis and tobacco use, contributing to evidence-based clinical practices.

3) Disease:
'Disease' signifies a pathological condition affecting the tissues, in this context, chronic periodontitis. This inflammatory disease affects the supporting structures of the teeth, leading to potential loss of teeth. Understanding disease mechanisms is vital for developing therapeutic strategies and improving management techniques, emphasizing the importance of early detection through cytomorphometric analysis.

4) Rules:
'Rules' denote the guidelines and protocols followed in conducting a scientific study, including ethical considerations. For instance, maintaining standards for participant selection, data collection, and analysis ensures reliable and valid findings. Adhering to established rules promotes integrity in research, ultimately benefiting scientific advancement and patient care.

5) Sharman (Sarma, Sharma, Sarman):
'Sharma' references a researcher or author whose work provides context or comparison related to cytomorphometric studies and changes in epithelial cells due to tobacco use. Such references lend credibility and connect the current study to previous findings, illustrating ongoing investigations in the field of dental research and cytology.

6) Post:
'Post' may refer to the 'post hoc' test used in statistical analysis to compare multiple groups after an analysis of variance (ANOVA). This step is crucial in determining the significance of differences between groups after finding an overall effect, helping to precisely interpret data and infer meaningful conclusions about the cytomorphometric changes observed.

7) Surface:
'Surface' pertains to the external layer of the oral mucosa where exfoliative cytology collects cells for analysis. Studying the surface cells provides insights into their morphology in health and disease conditions. This is critical for understanding cellular responses to pathologies, assisting in better diagnostics and therapeutic decisions.

8) Sign:
'Sign' often indicates the measurable manifestation of a physiological or pathological change, recognized during clinical or cytological observations. Identifying significant signs helps clinicians diagnose conditions earlier and tailor appropriate treatments, highlighting the need for innovative diagnostic tools that can reveal signs of disease progression at the cellular level.

9) Inflammation:
'Inflammation' refers to the body’s immune response to harmful stimuli like pathogens or irritants. In chronic periodontitis, inflammation damages supporting tissues of the teeth, leading to clinical symptoms. Understanding inflammation's role is vital for developing effective treatment strategies, allowing interventions that target inflammatory processes to mitigate damage during periodontal disease.

10) Discussion:
'Discussion' represents the section of a research manuscript where findings are interpreted, compared with existing literature, and contextualized. It is a crucial part of scientific writing, allowing authors to explain significance, implications, and limitations of their study, fostering future research directions and enhancing understanding of the studied phenomenon.

11) Irritation:
'Irritation' describes the overstimulation of tissues, often leading to inflammatory responses. In the context of chronic periodontitis and tobacco use, irritation may contribute to the worsening of periodontal conditions by altering cellular responses. Understanding irritation’s role helps elucidate mechanisms behind disease pathology, informing both prevention and therapeutic approaches.

12) Activity:
'Activity' in this context relates to the biological processes and functions of cells, especially regarding how they respond to pathological conditions. Increased activity can be indicative of cellular stress or transformation in disease states, and analyzing changes in cellular activity assists in understanding disease progression and patient behavior.

13) Pregnant:
'Pregnant' denotes a condition that could influence study participants' health and responses to treatments. Pregnant individuals often experience changes in oral health and may be more susceptible to periodontal diseases, making it necessary for research to exclude them to avoid confounding results caused by hormonal and physiological changes during pregnancy.

14) Science (Scientific):
'Science' embodies the systematic study of the natural world, relying on empirical evidence and experimental methodologies. In the context of dental research, applying scientific principles to understand chronic periodontitis and cellular changes emphasizes the need for rigorous research that contributes to evidence-based practices and advancements in oral health.

15) Mandal:
'Mandal' is most likely a reference to an author or institution involved in dental research. Citing prominent researchers like Mandal helps to connect current studies to established works, reinforcing findings and ideas. This collaboration of historical and contemporary research solidifies understanding and informs clinical applications in periodontics.

16) Medium:
'Medium' can refer to the method or material used to collect and preserve cellular samples. In this study, a 'medium nylon toothbrush' was utilized to gather gingival epithelial cells, emphasizing the importance of appropriate sampling techniques to ensure the quality and reliability of cytomorphometric analyses in oral research.

17) Filling (Filled):
'Filled' often describes the condition of cells within tissues, particularly how they might undergo changes due to pathological conditions. In the context of cytomorphometry, cells may exhibit signs of being filled due to fluid accumulation, thereby indicating cellular stress or damage, which is essential for analyzing disease-related changes in structures.

18) Nayak:
'Nayak' likely refers to an individual or researcher contributing to the context of the study, typically in leadership or administrative roles. Citing names such as Nayak helps highlight the institutional support for the research, reinforcing cooperative efforts in advancing scientific understanding within the fields of dentistry and health sciences.

19) Glass:
'Glass' refers to petri dishes or slides used in laboratory procedures to prepare and analyze cell samples. Glass slides allow clear visualization of cells under a microscope, which is essential for accurate cytomorphometric analysis. Proper handling and cleaning of glass materials ensure better results in cellular analysis.

20) Water:
'Water' indicates a common substance utilized to rinse the oral cavity to eliminate debris before sample collection. This step is vital in ensuring that collected cells are representative of the underlying conditions, underscoring the importance of preparatory procedures in obtaining quality specimens for cytomorphometric evaluation.

21) Blood:
'Blood' is a tissue involved in various medical diagnostics but is not the focus in this cytomorphometric study. It emphasizes the contrast between invasive blood tests and non-invasive methods like exfoliative cytology, showcasing how advancements in dental diagnostics offer alternatives that can be simpler and less discomforting for patients.

22) Sahu:
'Sahu' likely designates a researcher or author whose work draws comparisons or contextual references relevant to the study at hand. Incorporating works from researchers such as Sahu into the investigation strengthens the foundations of the study, builds upon existing knowledge, and rationalizes the need for ongoing research in the field.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Cytomorphometric study of gingival cells in healthy vs. periodontitis.’. Further sources in the context of Science might help you critically compare this page with similair documents:

Statistical analysis, Ethical Clearance, Chronic periodontitis, Papanicolaou stain, Forensic odontology, Pocket probing depth, Tobacco chewers, Image analyzer software, Systemic condition.

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