Early detection of dental plaque bacteria via MALDI-TOF MS.

| Posted in: Science

Journal name: Journal of Indian Society of Periodontology
Original article title: Early and accurate detection of bacterial isolates from dental plaque in subjects with primary, mixed, and permanent dentition by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry technique
The Journal of Indian Society of Periodontology (JISP) publishes original scientific articles on periodontology (the study of supporting structures of teeth) and oral implantology. It is a bimonthly open-access journal with special issues for specific occasions.
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Original source:

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Author:

Amitabh Srivastava, Sonali Saha, Chinmoy Sahu


Journal of Indian Society of Periodontology:

(A bimonthly open-access journal)

Full text available for: Early and accurate detection of bacterial isolates from dental plaque in subjects with primary, mixed, and permanent dentition by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry technique

Year: 2020 | Doi: 10.4103/jisp.jisp_303

Copyright (license): CC BY-NC-SA


Summary of article contents:

Introduction

The study investigates the bacterial colonization of dental plaques in different age groups—specifically those with primary, mixed, and permanent dentition—as it relates to dental disease prognosis. Utilizing the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) diagnostic technique, the research aims to accurately identify bacterial pathogens present in subgingival plaque samples collected from children aged 0-14. The study also highlights the importance of understanding the specific bacterial colonization patterns associated with different age groups to inform preventive and therapeutic approaches to periodontal diseases.

Highlight on Age-Related Bacterial Colonization

One critical finding from the study is the distinct age-specific colonization of bacteria in the oral cavity. In the primary dentition group, Streptococcus spp. were the predominant bacteria, while in the mixed and permanent dentition groups, orange complex bacteria like Prevotella nigrescens and red complex bacteria such as Porphyromonas gingivalis began to appear, indicating a shift towards more pathogenic species as children age. The data suggests that the likelihood of periodontal pathogens increases with age, emphasizing the need for targeted oral health interventions based on the identified bacterial populations at various stages of dentition. This information could be particularly useful for implementing effective dental care practices early on.

Conclusion

The study demonstrates that MALDI-TOF MS is a valuable tool for the rapid and precise identification of bacterial pathogens in dental plaques. Early detection and understanding of age-specific bacterial colonization patterns can enhance infection control measures, potentially preventing diseases such as periodontitis. As bacterial diversity correlates with increased periodontal disease risk, targeted strategies based on this understanding could ultimately lead to improved oral health outcomes. Further studies are necessary to deepen our knowledge of bacterial colonization dynamics and their implications for pediatric dental health.

FAQ section (important questions/answers):

What is the main purpose of the study?

The study aimed to evaluate the MALDI-TOF MS technique for identifying bacterial pathogens in dental plaques from subjects with primary, mixed, and permanent dentition, assessing age-related bacterial colonization.

How were the subjects grouped in the study?

The study included 150 subjects divided into three groups: Group A had 50 subjects with primary dentition (ages 0-5), Group B had 50 with mixed dentition (ages 6-11), and Group C had 50 with permanent dentition (ages 12-14).

What diagnostic method was used for bacterial identification?

The study utilized matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to accurately identify bacterial isolates from cultured dental plaque samples.

What were the predominant bacteria found in primary dentition?

In primary dentition, the predominant bacteria included Streptococcus spp. (51%), followed by Escherichia coli (19%) and Veillonella spp. (19%). These bacteria are primarily linked to early oral colonization.

What conclusions were drawn from the study's results?

The study found distinct age-specific bacterial colonization patterns, highlighting the effectiveness of MALDI-TOF MS in detecting periodontal pathogens, which can support proactive oral health programs.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Early detection of dental plaque bacteria via MALDI-TOF MS.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Table:
Table refers to a structured arrangement of data or information presented in rows and columns. In the context of this study, tables are used to summarize key demographics, bacterial species identified, and bacterial loads across different age groups. They allow for quick referencing and comparison of results, making complex data more understandable.

2) Study (Studying):
Study signifies a systematic investigation aimed at acquiring knowledge. In this context, the research focuses on evaluating the effectiveness of MALDI-TOF MS for identifying bacterial pathogens in dental plaques from subjects of varying dentition. It contributes to understanding the relation between bacterial colonization and dental health across different age groups.

3) Disease:
Disease refers to a pathological condition characterized by specific signs and symptoms. In this study, periodontal disease is particularly relevant, as the research investigates bacterial colonization in dental plaques which can lead to inflammation and infection of the gums, ultimately affecting oral health, especially in children.

4) Species:
Species denotes a group of organisms that can interbreed and produce fertile offspring. In this research, the identification of bacterial species from dental plaque is crucial, as different species have distinct pathogenic potentials. Understanding these species populations helps in associating them with potential oral health diseases and informs treatment approaches.

5) Surface:
Surface pertains to the outermost layer of something. In the context of this study, it refers to the buccal and lingual surfaces of teeth from which subgingival dental plaque samples were collected. The analysis of these surfaces helps determine the bacterial colonization pattern in different dentition groups.

6) Medium:
Medium indicates a substance in which organisms are cultured for growth in microbiological studies. In this research, specialized culture media, such as Robertson's Cooked Meat medium, are utilized to create an environment conducive for the growth of bacteria from collected dental plaque samples for further analysis.

7) Rules:
Rules signify established guidelines or principles. In the context of this study, they pertain to the standardized protocols followed during specimen collection, bacterial isolation, identification, and statistical analysis. Adhering to these rules ensures the reliability and validity of the research findings.

8) Male:
Male refers to the sex of an organism that produces small, mobile gametes, specifically sperm. In the context of this study, demographic details about male subjects are recorded to assess the gender distribution among the participants, helping to understand any potential differences in bacterial colonization across genders.

9) Hygiene (Hygienic):
Hygiene denotes practices related to cleanliness and health maintenance. In this research, the emphasis on oral hygiene is significant as it influences bacterial colonization and the risk of periodontal disease. Findings can inform recommendations for improved hygiene practices to maintain oral health in various age groups.

10) Blood:
Blood is a vital fluid that circulates in the bodies of vertebrates, providing essential nutrients and oxygen. In the study’s context, blood agar medium is used for culturing bacteria. It allows researchers to observe colony characteristics of bacteria, facilitating their identification and understanding of their health implications.

11) Miti:
Miti likely refers to 'Mitiss' or 'Mitus,' representing specific bacterial species, particularly *Streptococcus mitis*, that may be found in the oral cavity and dental plaques. This species could play a role in dental health and disease, showcasing the importance of identifying varied bacterial populations in dental assessments.

12) Post:
Post usually indicates an action or a condition following an event. In this study, it may refer to the post-examination analyses where researchers assess the implications of findings related to bacterial colonization in children’s oral health following different dental treatments or preventive care strategies.

13) Human body:
The human body refers to the complete physical structure of a human being. Understanding the microbial communities present in different regions of the human body, especially in the oral cavity, is important in the context of this study, as it establishes the basic premise for exploring bacterial colonization patterns.

14) Discussion:
Discussion refers to the analytical part of research where findings are interpreted in relation to existing literature. In this study, the discussion section explores how age-related bacteria colonization patterns correlate with dental health and treatment consequences, aiming to present a comprehensive understanding of bacterial impacts on periodontal conditions.

15) Antibiotic (Antibacterial):
Antibiotic signifies a class of medications used to prevent and treat bacterial infections. In the context of this study, the prior use of antibiotics by subjects is significant as it can affect bacterial populations in the oral cavity, influencing the study’s findings on bacterial diversity and colonization in dental plaques.

16) Filling (Filled):
Filled denotes the act of making something full or occupying space. In the context of this research, it refers to the process of placing dental plaque samples into sterile containers with reduced culture medium, minimizing exposure to air to preserve sample viability for accurate microbiological assessment and identification.

17) Birth:
Birth indicates the emergence of a new organism from its mother. In the scope of this study, it’s relevant as it highlights the starting point for the colonization of bacteria in a child’s oral cavity, setting the stage for understanding how bacterial populations evolve with age and affect dental health.

18) Labour (Labor):
Labor signifies physical or mental effort, especially in tasks like work or study. Within the context of this research, it pertains to the processes involved in collecting samples, culturing bacteria, and analyzing results. Understanding these efforts is important since they directly impact the study’s reliability and outcomes.

19) Genu:
Genu refers to a bend or knee-like structure, often used in anatomical contexts. Although less directly applicable to this study, it may metaphorically represent the varied complexity of interactions between bacterial species and host tissues, likening the dynamic relationship in the oral cavity to the bending and positioning of structures.

20) Meat:
Meat generally refers to animal flesh used for food. In the study's context, the term relates to the culture medium, Robertson's Cooked Meat medium, which is utilized to support bacterial growth. Such media are essential in microbiology to provide nutrients necessary for the growth of diverse microorganisms.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Early detection of dental plaque bacteria via MALDI-TOF MS.’. Further sources in the context of Science might help you critically compare this page with similair documents:

Informed consent, Ethical Clearance, Gingival index, Periodontal pathogens, Bacterial load, Bacterial colonization, Dental plaque samples, Clinical parameter, Conventional culture method, Microbiological method, High Prevalence, Oral microbiota, Red complex bacteria, Dentition groups, Bacterial growth assessment, Age-specific colonization, Complex bacterial interactions, Orange complex bacteria, Advanced diagnostic techniques, Transitional oral microbiota.

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