In vitro study of shiitake mushroom’s effect on periodontal pathogens.

| Posted in: Science Health Sciences

Journal name: Journal of Indian Society of Periodontology
Original article title: To detect the minimum inhibitory concentration and time-kill curve of shiitake mushroom on periodontal pathogens: An in vitro study
The Journal of Indian Society of Periodontology (JISP) publishes original scientific articles on periodontology (the study of supporting structures of teeth) and oral implantology. It is a bimonthly open-access journal with special issues for specific occasions.
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Original source:

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Author:

Shikha Sharma, Shobha Prakash


Journal of Indian Society of Periodontology:

(A bimonthly open-access journal)

Full text available for: To detect the minimum inhibitory concentration and time-kill curve of shiitake mushroom on periodontal pathogens: An in vitro study

Year: 2019 | Doi: 10.4103/jisp.jisp_249

Copyright (license): CC BY-NC-SA


Summary of article contents:

Introduction

Shiitake mushrooms (Lentinula edodes), a widely cultivated edible fungus originating from Asia, possess notable nutritional properties, including high levels of proteins, vitamins, and minerals. Research has highlighted the potential medical benefits of shiitake extracts, demonstrating antitumor, antiviral, antibiotic, and hypocholesterolemic actions. This study focuses on evaluating the inhibitory and antibacterial effects of shiitake mushroom extracts against periodontal pathogens, aiming to provide an alternative to conventional chemical treatments for oral biofilm elimination.

Evaluation of Antibacterial Efficacy

The investigators employed a double extraction method (hot water and ethanol) to assess the minimum inhibitory concentrations (MICs) of shiitake extracts on four significant periodontal pathogens: Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Prevotella intermedia. Results indicated that shiitake extracts displayed substantial antibacterial activity, with MIC values as low as 0.4 μg/ml for both P. gingivalis and P. intermedia, demonstrating their sensitivity. In contrast, A. actinomycetemcomitans and F. nucleatum exhibited higher MICs, suggesting varied sensitivity levels. Furthermore, time-kill analyses revealed that significant reductions in bacterial growth occurred within two hours of exposure to the extracts, indicating a strong bactericidal effect.

Conclusion

This study concludes that shiitake mushroom extracts possess significant antimicrobial properties against specific periodontal pathogens, potentially offering a natural alternative for periodontal disease management. Notably, the research emphasizes the promise of natural compounds in mitigating oral diseases while reducing reliance on traditional antibiotics, which may lead to resistance. Further exploration to isolate active compounds within shiitake and develop effective delivery mechanisms is warranted to optimize their therapeutic potential and applications in dental care.

FAQ section (important questions/answers):

What are the health benefits of shiitake mushrooms?

Shiitake mushrooms are rich in proteins, vitamins, and minerals, offering various health benefits such as boosting the immune system, promoting cardiovascular health, and possessing antimicrobial properties.

How were the antibacterial effects of shiitake mushrooms tested?

The study utilized hot water and ethanol extracts to determine minimum inhibitory concentrations (MIC) against specific periodontal pathogens using tube dilution and time-kill curve methods.

What were the results of the shiitake mushroom study?

Results showed that shiitake extracts exhibited potent antibacterial activity, with MIC values ranging from 0.4 μl to 12.5 μl for various periodontal pathogens, and effective growth inhibition over time.

Why are natural antimicrobial agents being researched?

Research is focused on finding safe, natural antimicrobial agents like shiitake extracts to prevent dental diseases, as conventional treatments may cause side effects and contribute to antibiotic resistance.

What further research is needed regarding shiitake mushrooms?

Further studies are necessary to isolate active antimicrobial compounds in shiitake mushrooms and to develop effective pharmaceutical delivery systems for optimal usage in dental health.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “In vitro study of shiitake mushroom’s effect on periodontal pathogens.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Table:
Table refers to a structured format for organizing and presenting data in a clear, concise manner. In scientific studies, tables display results such as minimum inhibitory concentrations (MIC) for various pathogens, facilitating comparison, analysis, and understanding of complex information, crucial for drawing meaningful conclusions from research data.

2) Study (Studying):
Study denotes the systematic investigation of a specific subject or phenomenon, often involving experiments or research. In the context of the text, it pertains to the research conducted on shiitake mushroom extracts and their effects on periodontal pathogens, aiming to understand antimicrobial properties and applications.

3) Disease:
Disease signifies a pathological condition that disrupts normal bodily functions, often caused by infections or environmental factors. The text discusses dental diseases, such as periodontal diseases, resulting from microbial imbalances in the oral cavity, highlighting the need for effective treatments to mitigate oral health issues.

4) Antibiotic (Antibacterial):
Antibiotic indicates a class of medications used to treat bacterial infections by inhibiting bacterial growth. The study contrasts traditional antibiotics with natural alternatives like shiitake extracts, addressing the risks of antibiotic resistance and advocating for safer, bioactive compounds in oral health management.

5) Water:
Water is a fundamental solvent essential for life, participating in various biological and chemical processes. In the study, distilled water is utilized to prepare aqueous extracts of shiitake mushrooms, key for analyzing the extracts' properties and efficacy against pathogens, highlighting its importance in scientific procedures.

6) Species:
Species denotes a category of living organisms that share common characteristics and can interbreed. The text refers to various bacterial species implicated in dental diseases, such as *Porphyromonas gingivalis* and *Aggregatibacter actinomycetemcomitans*, underscoring the relevance of understanding microbial diversity in disease treatment.

7) Powder:
Powder represents a finely ground solid substance. In this study, dried shiitake mushrooms are crushed into a powder to facilitate the extraction of bioactive compounds, crucial for preparing extracts that are tested for antimicrobial efficacy, demonstrating its significance in herbal medicine preparation.

8) Developing:
Developing refers to the process of progress or growth towards completion or improvement. In the text, it suggests the ongoing efforts in researching and formulating effective treatments for periodontal diseases utilizing shiitake extracts, reflecting the necessity of continual advancements in medical and dental fields.

9) Karnataka:
Karnataka is a state in India, providing context for the geographic and cultural background of the study. The text acknowledges institutions located in Karnataka, emphasizing collaborations in research and educational frameworks that support scientific endeavors like exploring shiitake mushroom extracts.

10) Surface:
Surface refers to the outermost layer of an object or organism. The text mentions dental surfaces, where bacteria accumulate to form biofilms contributing to diseases, emphasizing the significance of understanding microbial interactions at these surfaces for effective treatment and preventive strategies in dentistry.

11) Rules:
Rules imply established guidelines or principles governing conduct or actions. In the context of the study, this may refer to scientific methodologies and ethical practices in conducting research, signifying the importance of following established protocols to ensure valid and reliable results in scientific exploration.

12) Food:
Food denotes substances consumed to sustain life, providing necessary nutrients. The text discusses shiitake mushrooms as a food source, highlighting their nutritional and medicinal properties, indicating the dual role of edible products in both dietary health and potential therapeutic applications in treating diseases.

13) Drug:
Drug refers to a chemical substance used to diagnose, treat, or prevent diseases. In the context of this study, the term relates to the efficacy of shiitake mushroom extracts as natural drugs against periodontal pathogens, emphasizing the search for safer alternatives in medicinal treatments.

14) Accumulation (Accumulating, Accumulate):
Accumulate means to gather or increase gradually over time. In the study, it describes how bacteria accumulate in dental biofilms, leading to diseases, underscoring the importance of addressing these accumulations in managing periodontal health and informing treatment strategies for dental care.

15) Surrounding:
Surrounding refers to the environment or area around a specific location or object. In the study's context, it relates to the surrounding connective tissue involved in periodontal health, emphasizing the interconnectedness of oral structures and the influence of microbial activity on overall oral health.

16) Discussion:
Discussion denotes the section of a study where the results are interpreted and contextualized. It allows researchers to contrast findings with existing literature, draw conclusions, and highlight the significance of the study, facilitating an understanding of the implications of shiitake mushroom extracts.

17) Irritation:
Irritation refers to a state of inflammation or discomfort caused by external factors. The text mentions potential irritation caused by conventional antimicrobial treatments, highlighting the need for safer alternatives, like shiitake mushroom extracts, that can effectively combat pathogens without causing adverse effects.

18) Attacking:
Attacking indicates aggressive action aimed at harming or obstructing something. In the context of the text, it describes how periodontal diseases attack tooth-supporting structures, illustrating the destructive nature of these diseases and the crucial need for effective prevention and treatment strategies in dental health.

19) Flavonoid:
Flavonoid denotes a group of phytonutrients found in many fruits and vegetables, known for their antioxidant and potential health-promoting properties. In the study, it emphasizes the exploration of natural compounds, including flavonoids in shiitake mushrooms, and their role in improving oral health and combating pathogens.

20) Medicine:
Medicine refers to the science and practice of diagnosing, treating, and preventing diseases. The study situates itself within the field of medicine, specifically addressing dental and oral health, emphasizing the relevance of exploring natural products like shiitake mushrooms for innovative medical treatments.

21) Activity:
Activity signifies the function or behavior of an organism or substance. In the study, it can refer to the antimicrobial activity of shiitake mushroom extracts, assessing their effectiveness against specific pathogens and their potential therapeutic benefits in managing oral diseases.

22) Dividing:
Dividing entails separating or partitioning into parts. In this context, it refers to the process of preparing mushroom extracts by dividing dried powdered mushrooms for extraction, illustrating the meticulous methods used in preparing and analyzing natural compounds for medicinal evaluations.

23) Richness:
Richness denotes a high level or abundance of something. In the study, it highlights the richness of shiitake mushrooms in nutritional and bioactive compounds, which may contribute significantly to their potential health benefits and efficacy as natural remedies against periodontal pathogens.

24) Mineral:
Mineral refers to a naturally occurring inorganic substance essential for various bodily functions. In the text, it connects to the nutritional profile of shiitake mushrooms, indicating their potential contributions to overall health and supporting the exploration of their therapeutic applications in medicine.

25) Mandal:
Mandal refers to a subdivision within the administrative framework in India. In the text, it pertains to the region where some institutions contributing to the research are located, underscoring the collaboration between local academic entities in advancing scientific knowledge, particularly in natural products research.

26) Cancer:
Cancer denotes a group of diseases involving uncontrolled cell growth. While the study focuses on oral health, the mention of cancer emphasizes the broader health benefits of substances like shiitake mushrooms, highlighting their potential roles in cancer prevention and overall health promotion.

27) Animal:
Animal refers to multicellular organisms that can exhibit movement and response to stimuli. In the context of the study, it might relate to previous in vivo research exploring the health benefits of shiitake mushroom extracts in animal models, providing foundational insights for enhancing human health.

28) Field:
Field denotes a particular area of study or profession. Here, it relates to the fields of microbiology and dentistry, emphasizing the multidisciplinary nature of the research, exploring the intersection between food science, medicinal research, and oral health applications.

29) Miti:
Miti may refer to *Streptococcus mitis*, a bacterial species relevant in dental research. The mention indicates the significance of understanding various bacterial species implicated in oral diseases, which aligns with the study's exploration of antimicrobial interventions against periodontal pathogens.

30) Wall:
Wall signifies a structural barrier, often protecting or encasing an area. In microbiology, it refers to cell walls of bacteria, which are crucial for maintaining structural integrity. The study touches on how compounds like carvacrol disrupt bacterial cell walls, indicating their potential efficacy in antimicrobial treatments.

Other Health Sciences Concepts:

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Discover the significance of concepts within the article: ‘In vitro study of shiitake mushroom’s effect on periodontal pathogens.’. Further sources in the context of Health Sciences might help you critically compare this page with similair documents:

Gastrointestinal irritation, Minimum inhibitory concentration, Dental caries, Antimicrobial agent, Oxidative damage, Periodontitis, Bacterial resistance, Periodontal pathogens, Time-kill curve, Antibacterial efficacy, Natural bioactive compounds, Active compound, Microbial Communities, Oral biofilm, Dental biofilm, Antitumor action, Oral health benefits, Shiitake mushroom, Double extract, Pharmaceutical delivery system.

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