Fibroblast attachment on fluorosed vs. nonfluorosed teeth post-SRP.

| Posted in: Science Health Sciences Journals

Journal name: Journal of Indian Society of Periodontology
Original article title: Fluorosed fibroblast attachment on fluorosed and nonfluorosed teeth after SRP and EDTA root biomodification
The Journal of Indian Society of Periodontology (JISP) publishes original scientific articles on periodontology (the study of supporting structures of teeth) and oral implantology. It is a bimonthly open-access journal with special issues for specific occasions.
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Original source:

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Author:

Neha Girotra, K. L. Vandana


Journal of Indian Society of Periodontology:

(A bimonthly open-access journal)

Full text available for: Fluorosed fibroblast attachment on fluorosed and nonfluorosed teeth after SRP and EDTA root biomodification

Year: 2014 | Doi: 10.4103/0972-124X.128195

Copyright (license): CC BY-NC-SA


Summary of article contents:

Introduction

The study investigates the impact of dental fluorosis on fibroblast attachment to tooth root surfaces, a critical aspect of periodontal healing. Fluorosis, a condition caused by excessive fluoride intake, alters the mineral composition of dental tissues, potentially affecting the healing capacity of periodontal tissues. This research is necessary due to the growing prevalence of fluorosis, particularly in rural areas, and aims to enhance our understanding of its implications on periodontal health, especially regarding fibroblast activity on both fluorosed and nonfluorosed tooth roots.

The Importance of Fibroblast Attachment

Fibroblast attachment is essential for periodontal healing, as these cells play a significant role in tissue regeneration and repair. In this study, fibroblast attachment was evaluated on various treated root fragments, including those subjected to scaling and root planing (SRP) and 24% ethylenediaminetetraacetic acid (EDTA) gel application. The findings revealed that while SRP significantly enhanced fibroblast attachment in nonfluorosed roots, EDTA treatment did not yield positive results for fluorosed roots, indicating that the effectiveness of root conditioning methods may differ based on the fluorosed condition of the tooth surface. Furthermore, the presence of a smear layer could hinder fibroblast attachment, emphasizing the need for effective root preparation techniques to promote periodontal healing.

Conclusion

This study concludes that scaling and root planing is vital for fostering healthy fibroblast attachment in both fluorosed and nonfluorosed root surfaces. Despite the potential benefits associated with root biomodification using EDTA, its application did not demonstrate promising results for fluorosed roots in the given concentration and treatment duration. Future research should focus on analyzing the chemical composition and mineralization status of fluorosed cementum, aiming to understand the morphological changes in fluorosed fibroblasts and developing optimized treatment strategies for effective periodontal healing in patients affected by fluorosis.

FAQ section (important questions/answers):

What is the main objective of the study?

The main objective of the study was to evaluate the attachment of fluorosed fibroblasts to fluorosed and nonfluorosed root fragments, understanding the effects of fluorosis on periodontal healing.

How were the study groups categorized?

The study groups were categorized based on the health status of tooth roots (fluorosed and nonfluorosed) and whether they were treated with methods like scaling and root planing or EDTA.

What significant findings were observed in fibroblast attachment?

It was found that nonfluorosed diseased roots had greater fibroblast attachment compared to nonfluorosed healthy roots, while fluorosed roots showed no significant difference between groups.

What conclusion did the study reach regarding EDTA?

The study concluded that 24% EDTA does not effectively enhance fibroblast attachment on fluorosed roots, suggesting its limited application in root biomodification under the studied conditions.

What was the role of scaling and root planing in the study?

Scaling and root planing (SRP) was identified as a standard requirement for fibroblast attachment, necessary for healing, in both fluorosed and nonfluorosed root surfaces.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Fibroblast attachment on fluorosed vs. nonfluorosed teeth post-SRP.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Surface:
In the context of dental studies, 'surface' refers to the outermost layer of a tooth's root or cementum where fibroblast attachment occurs. The characteristics of this surface, including its texture and chemical composition, significantly influence the ability of cells to adhere and promote healing after periodontal disease treatments.

2) Study (Studying):
'Study' in scientific literature denotes a systematic investigation aimed at understanding a specific phenomenon or phenomenon's effects. In this context, the study evaluates how fluorosis affects fibroblast attachment to tooth roots. The methodology and findings contribute to advancing knowledge in periodontology and treatment efficacy.

3) Disease:
'Disease' refers to pathological conditions impacting health, like periodontal disease, which affects the structures surrounding teeth. Understanding disease mechanisms is crucial for developing effective treatment options. The study investigates how fluorosis, a common condition, may complicate healing processes associated with periodontal disease, influencing fibroblast behavior.

4) India:
'India' is significant in this context as it is the geographic location of the study. Fluorosis is endemic in several regions, particularly in Karnataka. Research conducted in India highlights the public health implications of fluoride exposure and seeks to gain insights specific to populations affected by fluorosis.

5) Line:
'Line' in this context can pertain to various concepts. It may refer to the methodological line followed during the study or a protocol developed for research. Additionally, it could suggest boundaries in scientific discourse, emphasizing the need for clear delineation of research objectives within the study.

6) Karnataka:
'Karnataka' is a state in southern India known for its high fluoride levels in groundwater. This region has a significant population affected by dental fluorosis, making it an essential location for studying the impacts of fluoride on oral health. The research aims to address these local public health challenges.

7) Medium:
'Medium' is often used in scientific literature to refer to the environment or substance in which cells are cultured. In this study, the 'medium' facilitates the growth of fibroblasts from periodontal tissues. It is crucial for experimentation, enabling the assessment of cellular attachment and behavior.

8) Water:
'Water' is a vital component in biological studies, often serving as a medium for cell culture and as a solvent for various biochemical processes. In the context of fluoride exposure and studies on dental fluorosis, the fluoride concentration in drinking water is a crucial factor influencing oral health.

9) Observation:
'Observation' refers to the act of monitoring or assessing phenomena during a study. In scientific research, careful observation provides essential data on experimental outcomes, such as fibroblast attachment to tooth surfaces. It supports validation of hypotheses and contributes to the overall understanding of results in the context of periodontal health.

10) Science (Scientific):
'Science' encompasses the systematic study of the structure and behavior of the physical and natural world through observation and experimentation. In this study, science is applied to understand the interactions between fluoride exposure and human physiological responses in the context of periodontal diseases and treatments.

11) Reason:
'Reason' signifies the justification or rationale behind conducting research. In this context, researchers are motivated by the need to explore the effects of fluorosis on fibroblast attachment due to its implications in periodontal healing. Understanding these relationships can improve treatment protocols and support public health initiatives.

12) Wall:
'Wall' may refer to cellular walls or anatomical barriers in microbiology and pathology. In this research, it could symbolize the boundary between healthy and affected tissues or even the anatomical barriers that influence cellular behavior and attachment on root surfaces following periodontal treatments.

13) Discussion:
'Discussion' is a section in scientific papers where authors interpret their findings, compare them with existing literature, and explain their significance. In this study, the discussion addresses the implications of the results regarding fibroblast attachment to teeth affected by fluorosis, highlighting areas for future research.

14) Antibiotic (Antibacterial):
'Antibiotic' refers to substances that inhibit bacterial growth and are used in the treatment of infections. In periodontal studies, antibiotics can be applied to manage infections associated with periodontal disease, highlighting the interplay between microbiology and treatment outcomes, particularly in the context of fluoride effects.

15) Activity:
'Activity' in biological research refers to the functions or behaviors exhibited by cells or biological processes. This study evaluates the activity of fibroblasts in response to fluoride exposure and treatment methods, providing insights into how these factors influence fibroblast attachment and healing in periodontal contexts.

16) Filling (Filled):
'Filling' typically refers to restorative materials used to treat cavities. In the context of periodontal research, filling materials may relate to restorative dental practices after extraction or periodontal procedures. Understanding the interactions between fillings and tissue responses can contribute to improved treatment methodologies in dentistry.

17) Animal:
'Animal' in scientific literature often represents subjects used for experimentation or study. This research, while focusing on human cells, may invoke comparisons with animal studies that serve to understand biological responses. The relevance includes ethical considerations and applicability of findings from animal models to human health.

18) Cotton:
'Cotton' in a laboratory context may refer to materials used for maintenance and handling procedures, maintaining aseptic conditions during experimentation. It may also be utilized in protocols such as applying EDTA or for clean-up, playing a crucial role in ensuring contamination-free environments in biological research.

19) Indian:
'Indian' relates to the context of this study being situated in India, particularly in endemic areas for dental fluorosis. Understanding the unique cultural, dietary, and environmental factors influencing fluoride exposure in Indian populations is vital for tailoring public health initiatives targeted toward fluorosis treatment and prevention.

20) Table:
'Table' often refers to organized data representations in research papers. It aids in summarizing results and findings, making it easier for readers to grasp complex information, such as the comparative data regarding fibroblast attachment across different treatment groups, enhancing comprehension and further analysis.

21) Blood:
'Blood' refers to the vital fluid carrying cells and nutrients in the body. In periodontal research, blood's role in inflammation and healing processes is critical. Understanding how blood components interact with periodontal tissues helps elucidate the mechanisms of disease and the function of cellular responses in healing.

22) Eagle:
'Eagle' in this context may not directly relate to the study but could symbolize a broader perspective of research, possibly alluding to the 'Eagle' technique in tooth extraction or referencing historical methodologies in dental research. It presents an opportunity to discuss advancements in dental science.

23) Hand:
'Hand' may refer to manual techniques or methodologies applied in dental procedures and research. It embodies the practical skills necessary for executing treatments, performing extractions, and conducting laboratory analyses, emphasizing the importance of human skill in achieving accurate results in dental studies.

Other Health Sciences Concepts:

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Discover the significance of concepts within the article: ‘Fibroblast attachment on fluorosed vs. nonfluorosed teeth post-SRP.’. Further sources in the context of Health Sciences might help you critically compare this page with similair documents:

Statistical analysis, Public Health Problem, In vitro study, Dental caries, Clinical manifestation, Diabetic control, Scanning Electron Microscopy, Scaling and root planing, Fluorosis, Endemic fluorosis, Morphologic alterations, Fibroblast attachment, Scaling procedure.

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