PCR vs. culture for identifying Tannerella forsythia in periodontal health
Journal name: Journal of Indian Society of Periodontology
Original article title: Comparison of culture and polymerase chain reaction techniques in the identification of Tannerella forsythia in periodontal health and disease, an in vitro study
The Journal of Indian Society of Periodontology (JISP) publishes original scientific articles on periodontology (the study of supporting structures of teeth) and oral implantology. It is a bimonthly open-access journal with special issues for specific occasions.
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Praveen Kumar Bankur, Aarati Nayak, Kishore Bhat, Rashmi Bankur, Reshma Naik, Nami Rajpoot
Journal of Indian Society of Periodontology:
(A bimonthly open-access journal)
Full text available for: Comparison of culture and polymerase chain reaction techniques in the identification of Tannerella forsythia in periodontal health and disease, an in vitro study
Year: 2014 | Doi: 10.4103/0972-124X.131312
Copyright (license): CC BY-NC-SA
Summary of article contents:
Introduction
This study investigates the prevalence of Tannerella forsythia (Tf), a significant bacterium related to the progression of periodontitis, in subgingival plaque samples from both periodontally healthy individuals and chronic periodontitis patients. Utilizing culture and polymerase chain reaction (PCR) techniques, researchers aimed to determine the effectiveness of these methods in detecting Tf and compare their results. A total of 100 subjects were analyzed, divided equally between healthy participants and those with chronic periodontitis. This research is particularly relevant in light of the limited data available regarding the association of Tf with periodontal diseases in the Indian population.
Prevalence Detection Techniques
The results revealed notable differences in the prevalence of Tf detected by culture and PCR methods. Specifically, the culture technique identified Tf in 14% of healthy samples and 34% of periodontitis samples, whereas the PCR technique detected it in 20% of healthy samples and 40% of periodontitis samples. Statistical analysis confirmed that the prevalence of Tf was significantly higher in periodontitis patients compared to healthy subjects by both methods (P = 0.019 for culture; P = 0.029 for PCR). Furthermore, despite the culture method providing lower detection rates, it remained valuable for its ability to detect multiple bacterial species and ascertain antibiotic resistance, highlighting the complementary roles of both techniques.
Conclusion
In conclusion, this study establishes that T. forsythia is more prevalent in individuals with chronic periodontitis compared to healthy individuals, as evidenced by both culture and PCR techniques. PCR demonstrated a higher sensitivity (79.1%) for detecting Tf, suggesting its effectiveness as a diagnostic tool in periodontal research. However, the study emphasizes that while PCR can provide rapid and sensitive results, the culture technique should not be dismissed due to its unique advantages in broader microbiological analysis. Overall, this research contributes to the understanding of microbial dynamics in periodontal disease within the Indian context, underscoring the need for further studies to explore the prevalence of various periodontal pathogens.
FAQ section (important questions/answers):
What was the main objective of the study on Tannerella forsythia?
The study aimed to detect the presence of Tannerella forsythia in subgingival plaque from periodontally healthy and chronic periodontitis patients. It also compared the efficacy of culture and PCR techniques for this detection.
What techniques were used to detect Tannerella forsythia in the study?
The research utilized two methods: a culture technique and polymerase chain reaction (PCR) to detect the presence of T. forsythia in pooled subgingival plaque samples from participants.
What were the sample sizes in the study for both groups?
A total of 100 subjects were included, with 50 periodontally healthy individuals and 50 chronic periodontitis patients, from whom subgingival plaque samples were analyzed.
Which detection method showed higher sensitivity for Tannerella forsythia?
The PCR method demonstrated higher sensitivity (79.1%) compared to the culture technique (63.3%) in detecting Tannerella forsythia in subgingival plaque samples.
What was the conclusion regarding Tannerella forsythia in periodontitis patients?
The study concluded that T. forsythia was more prevalent in chronic periodontitis patients than in healthy individuals, with significant differences in detection rates between both groups.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “PCR vs. culture for identifying Tannerella forsythia in periodontal health”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Table:
In research, a table organizes data systematically, allowing readers to easily compare and analyze findings. It presents information like sample sizes, results, and statistical significance. In this context, tables provide crucial insights into the prevalence of Tannerella forsythia in different groups, enabling clear visual representation of results and facilitating understanding of the study's outcomes.
2) Study (Studying):
A study examines a specific question or hypothesis through systematic investigation. In this context, the study aimed to detect Tannerella forsythia in subgingival plaque among different groups. It involved rigorous methodologies and analyses, contributing valuable data to the understanding of periodontal diseases and the potential role of this bacterium in oral health.
3) Disease:
Disease refers to any abnormal condition affecting an organism, particularly concerning health. In this scenario, periodontitis is highlighted as a significant gum disease linked to the presence of specific bacteria, such as Tannerella forsythia. Identifying bacteria associated with periodontitis helps in understanding disease mechanisms and developing effective treatment interventions.
4) Species:
Species represent groups of organisms that can interbreed and produce fertile offspring. The term is relevant because Tannerella forsythia is a specific bacterial species investigated for its role in periodontal disease. Understanding the characteristics and behaviors of specific species is crucial in microbiology and helps establish their impact on human health.
5) Water:
Water is essential for life and plays a significant role in various biological processes. In microbiological studies, it often serves as a solvent during laboratory procedures, including sample transport and PCR processes. Its purity and quality are critical to prevent contamination and ensure accurate results in detecting organisms like Tannerella forsythia.
6) Indian:
The term Indian refers to the nationality or cultural context of the study participants. In research, the population's geographical and cultural specifics can influence microbial prevalence and health conditions. Understanding the role of Tannerella forsythia within the Indian population enhances knowledge of regional variability in periodontal disease and bacterial associations.
7) Bleeding:
Bleeding in dental contexts often signifies gum health issues, such as gingivitis or periodontitis. In this study, the presence of bleeding on probing was a criterion for diagnosing chronic periodontitis. Observations related to bleeding aid in assessing the severity of periodontal disease and the patient's overall oral health status.
8) Blood:
Blood is a vital fluid in the body that transports oxygen, nutrients, and waste products. In microbiological assays, blood components serve as culture media for growing certain bacteria like Tannerella forsythia. Its role in laboratory settings highlights the intersection between bodily fluids and microbial research, particularly in diagnosing diseases.
9) Antibiotic (Antibacterial):
Antibiotics are medications that combat bacterial infections. The study excluded subjects on antibiotics within three months to avoid skewed results regarding Tannerella forsythia prevalence. Understanding the impact of antibiotic use is crucial in managing periodontal diseases, as these medications can significantly alter the oral microbiome and affect detection rates.
10) Science (Scientific):
Scientific pertains to systematic methodologies based on empirical evidence and observation. This term emphasizes the rigor and structure of the research applied in studying Tannerella forsythia, ensuring that findings contribute to broader scientific knowledge of periodontitis and associated bacterial interactions, thereby enhancing dental microbiology and pathology.
11) Mandal:
Mandal refers to administrative regions in India, implying the research focus within a defined geographic context. This designation underscores the study's local relevance and highlights the need for research tailored to specific populations, considering different socio-economic and environmental factors affecting health outcomes and microbial diversity.
12) Hand:
Hand signifies the manual aspect of clinical examinations and specimen collection in research. In this context, trained dentists or researchers used sterile curettes to obtain subgingival plaque samples physically. The involvement of skilled practitioners ensures accuracy and reliability in data collection, which is crucial for the integrity of study results.
13) Inflammation:
Inflammation is the body's response to injury or infection, often characterized by redness, swelling, and pain. In periodontal disease contexts, inflammation in gum tissues is a critical indicator of health status. Understanding Tannerella forsythia's role in the inflammatory response enhances insights into the pathogenesis of periodontal diseases.
14) Observation:
Observation refers to the systematic noting of phenomena during research. In this study, observations regarding the presence of Tannerella forsythia in various sample groups contribute significantly to hypothesis testing and validating findings. Accurate observations are crucial to understanding the relationships between microbial presence and periodontal health or disease.
15) Discussion:
Discussion encompasses the interpretation and analysis of research findings in the context of existing literature. In this study, the discussion section connects the results regarding Tannerella forsythia's prevalence with broader implications for periodontal disease research, highlighting similarities, differences, and future directions for study within the scientific community.
16) Developing:
Developing refers to countries or regions that are undergoing economic or social progress. In this study, the focus on the Indian population as a developing context emphasizes the need for research that addresses dentistry and health issues unique to these regions, particularly in understanding microbial influences on oral disease.
17) Similarity:
Similarity denotes the degree to which two or more entities share characteristics. In this study, similarities in the detection of Tannerella forsythia across different studies may point to consistent patterns or trends in periodontal disease. Analyzing these similarities strengthens the understanding of bacterial behavior within various populations.
18) Knowledge:
Knowledge refers to the information gained through study and experience. In scientific research, expanding knowledge about microorganisms like Tannerella forsythia contributes to understanding periodontal diseases' etiology and prevention, informing clinical practices and enhancing public health policies regarding oral care in various populations.
19) Substance:
Substance typically refers to a specific material or compound. In the context of research, it often relates to the biochemical components within biological samples. Identifying substances that may inhibit or promote bacterial growth, like those found in plaque samples, is crucial for developing effective treatment strategies for periodontal disease.
20) Pregnant:
Pregnant refers to the state of carrying developing offspring within the womb. In clinical research, pregnant individuals often face additional health considerations that can influence study outcomes. Excluding pregnant subjects from this research was necessary to ensure any findings regarding Tannerella forsythia prevalence are not confounded by the unique physiological changes during pregnancy.
21) Reason:
Reason denotes the underlying rationale for specific choices or actions in research. Each aspect of the study's design, including participant exclusion, methodological approaches, and analytical frameworks, is driven by clear reasoning to ensure valid results regarding Tannerella forsythia. Understanding these reasons is essential for assessing research credibility and relevance.
22) Kamma:
Kamma refers to a surname indicating the work of Key Kamma et al. likely cited in the context of prevalence studies related to Tannerella forsythia. Citing specific research contributes to a comprehensive discussion, contextualizing current findings within a broader academic framework and acknowledging previous contributions to the field.
23) India:
India is a country with a diverse population and health challenges. Conducting research in this context allows for an understanding of specific microbial interactions within Indian populations regarding periodontal disease. The findings regarding Tannerella forsythia help address regional health disparities and contribute to global dental health research.
24) Storm:
Storm signifies a finding related to the study by Jervoe Storm et al., which reported high prevalence rates of Tannerella forsythia. References to previous studies bolster the credibility of new research by situating it within established scientific discourse, enabling comparisons and identifying patterns in microbial behavior across different populations.
25) Death:
Death refers to the cessation of life, an eventuality that all organisms face. In a medical context, it emphasizes the stakes associated with untreated diseases like periodontitis, which can lead to systemic health issues. Understanding microbial roles in such diseases helps avert serious health implications that could culminate in death.
26) Birth:
Birth signifies the beginning of life, marking an individual's entry into the world. In health studies, it often represents the initial state of an organism's microbial community. Recognizing how microbial compositions shift from birth through life stages informs the relationship between oral microbiome development and health status.
27) Sign:
Sign refers to an indication or evidence of a condition. In clinical research, signs are critical markers used to assess health. In this study, signs like Tannerella forsythia detection can indicate periodontal status, serving as vital evidence for researchers and clinicians in evaluating and managing oral health conditions.
Other Science Concepts:
Discover the significance of concepts within the article: ‘PCR vs. culture for identifying Tannerella forsythia in periodontal health’. Further sources in the context of Science might help you critically compare this page with similair documents:
Informed consent, Statistical analysis, Ethical committee, Systemic disease, Plaque index, Chi square test, Positive control, Gingival Bleeding Index, Negative control, Probing pocket depth, Clinical attachment level, Gel electrophoresis, PCR technique, Ethidium bromide, High sensitivity, Chronic periodontitis patients, Molecular diagnostic assays, Population prevalence, Blood agar plate, Subgingival plaque sample.