Salivary protein, flow, buffer, and pH in young vs elderly with gum issues.

| Posted in: Science

Journal name: Journal of Indian Society of Periodontology
Original article title: Salivary protein concentration, flow rate, buffer capacity and pH estimation: A comparative study among young and elderly subjects, both normal and with gingivitis and periodontitis
The Journal of Indian Society of Periodontology (JISP) publishes original scientific articles on periodontology (the study of supporting structures of teeth) and oral implantology. It is a bimonthly open-access journal with special issues for specific occasions.
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Original source:

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Author:

Mulki Shaila, G. Prakash Pai, Pushparaj Shetty


Journal of Indian Society of Periodontology:

(A bimonthly open-access journal)

Full text available for: Salivary protein concentration, flow rate, buffer capacity and pH estimation: A comparative study among young and elderly subjects, both normal and with gingivitis and periodontitis

Year: 2013 | Doi: 10.4103/0972-124X.107473

Copyright (license): CC BY-NC-SA


Summary of article contents:

Introduction

This study aimed to evaluate salivary protein concentrations in patients with gingivitis and periodontitis, comparing various parameters such as total protein, albumin, salivary flow rate, pH, and buffer capacity between young and elderly subjects. Utilizing simple biochemical methods, the researchers enlisted 120 participants, divided into young and elderly groups with subgroups consisting of controls and those afflicted with gingivitis or periodontitis. The methodology involved collecting unstimulated whole saliva and measuring flow rates, protein levels through the Biuret method, albumin through the Bromocresol green method, pH with a pH meter, and buffering capacity via titration.

Rise in Salivary Proteins in Inflammation

A key finding indicates a significant increase in salivary total protein and albumin concentrations associated with gingivitis and periodontitis across both age groups. Specifically, the average total protein concentration in controls, gingivitis, and periodontitis was found to be 0.86 g/mL, 1.19 g/mL, and 1.59 g/mL, respectively, and the increases were statistically significant (P=0.001). The study highlighted that these elevated protein levels likely stem from an enhanced secretion response due to inflammation, suggesting that salivary proteins can serve as effective biomarkers for diagnosing periodontal diseases. Additionally, variations in flow rates demonstrated significant gender differences, with males exhibiting higher salivary flow than females.

Conclusion

The findings underline the potential of using salivary total protein and albumin levels as simple, non-invasive biomarkers for identifying gingivitis and periodontitis in patients. Although the study has established associations between salivary protein concentrations and periodontal conditions, future longitudinal research is necessary to solidify the role of saliva in prognostic assessments. Additionally, the observed differences in salivary flow rates between genders and age groups may offer new insights into oral health and its relationship with systemic conditions.

FAQ section (important questions/answers):

What was the primary aim of the study on salivary proteins?

The study aimed to evaluate and compare salivary total protein, albumin, flow rate, pH, and buffering capacity in young and elderly patients with gingivitis and periodontitis using simple biochemical methods.

How was salivary protein concentration measured in the study?

Salivary protein was estimated using the Biuret method, where saliva was mixed with a reagent to form a colored complex, measured at a specific wavelength using a photoelectric colorimeter.

What significant differences were found between young and elderly groups?

A significant decrease in salivary flow rate was observed in elderly individuals, with women showing lower flow rates than men, indicating gender and age-related variations.

What were the findings regarding salivary albumin concentrations?

There was a very highly significant rise in salivary albumin concentrations in both gingivitis and periodontitis subjects compared to controls, suggesting its role as a marker for these conditions.

Was there a correlation between salivary parameters and periodontal disease?

Yes, the study found significant associations between salivary total protein and albumin levels as biochemical markers for periodontal diseases in both young and elderly subjects.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Salivary protein, flow, buffer, and pH in young vs elderly with gum issues.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Study (Studying):
The term 'study' refers to a systematic investigation undertaken to gather data and draw conclusions. In the context of the provided text, it emphasizes the research aimed at understanding the salivary protein concentrations in patients with gingivitis and periodontitis, highlighting its scientific significance in the field of dental medicine.

2) Disease:
Disease refers to a pathological condition of a bodily part resulting in abnormal functioning. The text discusses gingivitis and periodontitis, explaining their status as common oral diseases characterized by inflammation, indicating that understanding their biochemical markers can assist in diagnosis and treatment.

3) Table:
In research, a table is a method of systematically organizing data, making it easier to understand and analyze. The tables in the study highlight the biochemical values and differences among age groups and periodontal health statuses, supporting the statistical comparisons necessary for valid conclusions.

4) Inflammation:
Inflammation is a biological response to harmful stimuli, often signifying underlying issues like infection or injury. The text links inflammation to gingivitis and periodontitis, emphasizing its role in altering salivary protein levels, which serve as biomarkers for assessing oral health conditions.

5) Glass:
In the context of the text, 'glass' most likely refers to the materials used in laboratory instruments, such as pH meters. Glass probes, crucial for accurate measurements, reflect the precision required in biochemical analysis, underscoring the importance of reliable data collection in scientific studies.

6) Bleeding:
Bleeding is an important clinical indicator of periodontal disease. The text refers to the Gingival Inflammation Index and how bleeding on probing signifies the presence of gingivitis, directly relating to the adjusted salivary protein levels and highlighting its relevance as a marker for periodontal disease.

7) Science (Scientific):
Science is the systematic pursuit of knowledge through observation and experimentation. The study reflects this as it employs biochemical methods to investigate salivary proteins related to periodontal diseases, contributing to the broader understanding of oral health and disease mechanisms in a scientific framework.

8) Medium:
Medium relates to a means by which something is communicated or expressed. In the text, saliva is described as a non-invasive diagnostic medium for assessing oral diseases, illustrating its significance in conveying valuable physiological information relevant to gingivitis and periodontitis.

9) Blood:
Blood serves as a vital bodily fluid that transports nutrients and oxygen. The text discusses plasma protein leakage in gingivitis and periodontitis, noting that increased levels of salivary proteins, such as albumin, may stem from vascular processes akin to those occurring in blood, establishing a connection between systemic health and oral diseases.

10) Male:
The word 'male' denotes one of the two primary biological sexes. The study indicates differences in salivary flow rates between genders, noting that males had a higher flow rate, which contributes to understanding gender-related variations in oral health and disease susceptibility.

11) Surrounding:
Surrounding refers to the physical environment or context in which phenomena occur. In the text, the surrounding biological conditions affect the salivary composition and inflammatory responses in periodontal disease, emphasizing the interconnectedness of systemic and oral health regarding factors like microbial presence.

12) Xerostomia:
Xerostomia is a condition characterized by dry mouth due to reduced saliva. The text mentions it may develop in menopausal women, highlighting the relationship between hormonal changes, salivary function, and its potential impact on oral health, particularly in older populations.

13) Swallowing:
Swallowing is the act of moving food or liquid from the mouth to the stomach. In the research context, it refers to the method of saliva collection where subjects are instructed not to swallow, ensuring that the saliva sample accurately reflects the biochemical composition relevant to the study's aims.

14) Medicine:
Medicine is the science and practice of diagnosing, treating, and preventing disease. The study contributes to the field of dentistry, specifically periodontology, by investigating salivary changes in response to disease, thus it intersects medicine and oral health to improve patient care.

15) Activity:
Activity in this context references biological or physiological processes. The study assesses the biochemical activity within the saliva of patients with periodontal diseases, highlighting how changes in salivary proteins serve as indicators of inflammatory processes and disease progression.

16) Surface:
Surface denotes the outermost layer or boundary of an object. In dental contexts, the surfaces of teeth and gums interact with saliva and are critical in the development of gingival disease, indicating how protective factors, such as salivary proteins, play roles in overall dental health.

17) Kumari:
Kumari refers to Dr. Sucheta Kumari, acknowledged in the study for her role in guiding the biochemical analyses. Her contribution underscores the importance of expert collaboration in research, enhancing the study's credibility and accuracy in exploring biochemical markers for periodontal disease.

18) Seat (Seated):
Seated describes the position of subjects during saliva collection in the study. It indicates a controlled environment conducive to obtaining samples, reflecting the importance of standardization in research methodologies to ensure reliable and consistent results in scientific investigations.

19) Water:
Water is an essential component of saliva and plays a critical role in maintaining oral health. The text indirectly speaks to its importance by discussing salivary flow rates and the physiological properties of saliva, suggesting that proper hydration influences overall salivary function and health.

20) Drama:
Drama, in this context, connotes a lack of overt excitement or visual appeal. The text contrasts saliva with more traditionally engaging bodily fluids, emphasizing the often-overlooked significance of saliva in diagnostic processes, particularly for oral health evaluation and disease monitoring.

21) Field:
Field refers to a specific area of study or practice. The text is framed within the field of dental and periodontal research, demonstrating how investigations into salivary parameters can enhance our understanding of oral diseases and promote advancements in patient care within a clinical context.

22) Sweating (Sweat):
Sweat is a bodily secretion that serves thermoregulatory functions. The reference in the text serves to highlight various bodily fluids while positioning saliva as a crucial yet underappreciated fluid in clinical diagnostics, emphasizing the potential insights saliva offers in assessing health conditions.

23) Food:
Food denotes substances consumed to provide nutritional support. The text alludes to its relationship with salivary secretion and composition, hinting at how the types of food might affect oral health and the biochemical properties of saliva in patients with periodontal diseases.

24) Tear:
Tear refers to the fluid secreted by the lacrimal glands, often associated with emotion. This mention illustrates the wide range of bodily fluids with diagnostic potential, equating the less dramatic saliva to other more expressive fluids, thereby accentuating its role in health assessments.

25) Salt (Salty):
Salt is a compound commonly used in various biological processes, including maintaining fluid balance. In the context of saliva and the Biuret method described in the text, it underscores the significance of ionic solutions in biochemical reactions, emphasizing precise conditions required for accurate analyses.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Salivary protein, flow, buffer, and pH in young vs elderly with gum issues.’. Further sources in the context of Science might help you critically compare this page with similair documents:

Informed consent, Ethics Committee approval, Oral cavity, Gingivitis and periodontitis, Biuret Method, Gingival crevicular fluid, Bromocresol Green method, Future direction, Salivary flow rate, Gender Variation, BUFFER CAPACITY.

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