Sinus lift with autologous pulp micro-grafts: bone density case report

| Posted in: Science Health Sciences Journals

Journal name: Journal of Indian Society of Periodontology
Original article title: Sinus lift tissue engineering using autologous pulp micro-grafts: A case report of bone density evaluation
The Journal of Indian Society of Periodontology (JISP) publishes original scientific articles on periodontology (the study of supporting structures of teeth) and oral implantology. It is a bimonthly open-access journal with special issues for specific occasions.
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Original source:

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Author:

Giorgio Brunelli, Alessandro Motroni, Antonio Graziano, Riccardo D’Aquino, Ilaria Zollino, Francesco Carinci


Journal of Indian Society of Periodontology:

(A bimonthly open-access journal)

Full text available for: Sinus lift tissue engineering using autologous pulp micro-grafts: A case report of bone density evaluation

Year: 2013 | Doi: 10.4103/0972-124X.119284

Copyright (license): CC BY-NC-SA


Summary of article contents:

Introduction

The study investigates the potential of using dental pulp stem cells (DPSCs) for bone regeneration, specifically in a clinical setting involving sinus lift procedures. Traditional methods often rely on autografts for bone grafting, which can be limited by donor site morbidity. The Rigenera Protocol presents a novel approach to utilizing DPSCs sourced from dental pulp, which is more accessible and carries a lower risk of complications during collection. This report highlights the techniques and outcomes associated with the application of this method in a patient requiring bone augmentation in the maxilla.

The Promise of Dental Pulp Stem Cells

One of the key findings from this study is the notable effectiveness of micro-grafts obtained from dental pulp, which, when combined with collagen sponges, demonstrated significantly improved bone density compared to the native bone. The Rigenera Protocol allows for the extraction of viable micro-grafts, filtering out older differentiated cells to enrich for young progenitor cells. The computed tomography analysis conducted four months post-surgery showed that the bone density of the newly formed tissue was approximately double that of the native bone, suggesting a high regenerative capacity of DPSCs. This emphasizes the importance of utilizing dental pulp as a reliable source of stem cells that can facilitate effective bone regeneration in dental procedures.

Conclusion

The positive results of this case report indicate that the application of DPSCs derived through the Rigenera Protocol can serve as a viable alternative to traditional bone grafting methods. By leveraging the unique properties of dental pulp, clinicians can potentially achieve better outcomes in bone regeneration with reduced morbidity. Although this study is based on a single patient case, it lays the groundwork for further research into the use of DPSCs in clinical practice, offering hope for innovative strategies in regenerative dentistry and oral surgery.

FAQ section (important questions/answers):

What is the focus of this study on bone grafting?

This study explores the use of dental pulp stem cells as an alternative to traditional autografts for bone regeneration, specifically using micro-grafts in sinus lift procedures.

How were the dental pulp stem cells collected in this procedure?

Dental pulp stem cells were collected after extracting the upper right third molar and were dissociated using the Rigenera System, producing a cellular suspension for use in bone regeneration.

What results were observed regarding bone density after the procedure?

CT scan analysis showed that the bone density of the newly formed bone was approximately double that of the native bone, indicating successful regeneration.

What are the potential benefits of using dental pulp stem cells?

Dental pulp stem cells offer an accessible source of multipotent cells with minimal morbidity, reducing complications associated with harvesting from more invasive sites, making them suitable for therapeutic applications.

What is the Rigenera Protocol and its significance?

The Rigenera Protocol is a technique that disaggregates dental pulp tissue into micro-grafts without extensive manipulation, enhancing the clinical application of dental pulp for regenerative therapies.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Sinus lift with autologous pulp micro-grafts: bone density case report”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Surface:
The surface in the context of tissue engineering and regenerative medicine refers to the outer part of biomaterials that interact with cells. Textured surfaces can influence cell behavior, affecting adhesion, differentiation, and overall regenerative capability. They play a crucial role in guiding stem cell responses and promoting effective tissue integration.

2) Table:
In scientific research, a table is a systematic arrangement of data, typically used to present findings clearly. In this study, it displays median bone density for different types of bone. Tables are essential for data visualization, enabling quick comparisons and statistical analyses, aiding in drawing meaningful conclusions from research.

3) Medium:
The term medium refers to the environment or materials in which cells are cultured or grown. In the context of dental pulp stem cell research, differentiation and growth are enhanced by selecting appropriate growth media containing nutrients, hormones, and signaling molecules essential for maintaining cell viability and promoting desired cellular behaviors.

4) Human body:
The human body is a complex system comprising various tissues and organs, including niches containing stem cells. Such niches, like dental pulp, are crucial for therapies involving regenerative medicine as they provide accessible sources for obtaining stem cells with potential for treating various ailments and facilitating tissue repair.

5) Pharmacological:
Pharmacological refers to the branch of medicine that studies drug interactions and their effects on biological systems. In this study, pharmacological interventions, like antibiotics and anti-inflammatory medications, are vital for managing postoperative complications, preventing infections, and ensuring patient safety during and after surgical procedures involving stem cell grafting.

6) Performance:
Performance refers to the functional capability of cells or materials in achieving desired outcomes. In this context, the performance of dental pulp stem cells, particularly their ability to proliferate, differentiate, and integrate with existing bone structures, is crucial for ensuring effective regeneration and fulfilling clinical objectives in reconstructive therapies.

7) Antibiotic (Antibacterial):
Antibiotics are substances used to prevent or treat bacterial infections. In this study, antibiotics are administered post-surgery to prevent infections that could compromise the regenerative process of bone grafts. Their use is essential in surgical protocols to promote healing and safeguard patient health during tissue regeneration treatments.

8) Medicine:
Medicine is the science and practice of diagnosing, treating, and preventing disease, focusing on healing and patient care. In the context of this study, advances in regenerative medicine through the application of stem cells highlight the potential for new therapeutic approaches in dentistry and other fields, promoting healing and tissue regeneration.

9) Activity:
Activity, in this context, refers to the biological processes that occur within cells, particularly regarding their interaction with biomaterials and surrounding environments. The activity of dental pulp stem cells is crucial for their functionality in regenerative applications, encompassing proliferation, differentiation, and factors' secretion necessary for effective tissue repair and growth.

10) Incision:
An incision is a surgical cut made in the skin or tissue, allowing access to underlying structures. In the described surgical procedure, incisions are crucial for accessing the maxillary sinus for bone graft placement. Proper incision techniques are vital for minimizing trauma, promoting healing, and ensuring the success of surgical interventions.

11) Grafting:
Grafting refers to the process of transplanting tissue from one site to another to facilitate healing or regeneration. In this study, grafting involves using dental pulp-derived micro-grafts to augment bone in the maxillary sinus. This innovative approach aims to enhance bone regeneration and improve outcomes in implant-prosthetic therapy.

12) Channel:
Channel typically refers to a passage through which substances, including fluids or cells, can move. In the context of bone biology, channels are essential for vascularization and nutrient flow within regenerated tissues. Maintaining proper channels is vital for promoting bone integration and tissue health in regenerative therapies.

13) Quality:
Quality in biomedical contexts often refers to the effectiveness and characteristics of regenerated tissues, including structure, function, and integration. Ensuring high quality of regenerated bone tissue involves utilizing effective methodologies, such as employing dental pulp stem cells and appropriate scaffolding techniques, which facilitate robust healing and functional results in clinical outcomes.

14) Disease:
Disease refers to health impairments that impact normal bodily functions. Understanding disease mechanisms is essential for developing therapeutic strategies. In regenerative medicine, diseases affecting bone structures can benefit from approaches that leverage stem cells for tissue regeneration and repair, particularly in oral and maxillofacial contexts.

15) Science (Scientific):
Science is the systematic pursuit of knowledge through observation and experimentation. In the realm of regenerative medicine, scientific principles guide research and application of technologies, such as stem cells from dental pulp, to facilitate innovative treatments that can significantly impact tissue healing and regeneration, enhancing patient outcomes.

16) Repair:
Repair encompasses the biological processes that restore tissue integrity and function following injury or damage. In regenerative medicine, the goal is to enhance the repair of damaged structures, such as bone in the maxilla, utilizing stem cells to stimulate cell growth and promote effective healing in clinical applications.

17) Filling (Filled):
Filled denotes the act of occupying a space, often referring to the placement of materials within biological contexts. In this study, the sinus cavity is filled with a mixture of collagen sponge and dental pulp micro-grafts, vital for supporting regenerative processes and ensuring suitable conditions for bone healing and integration.

18) Water:
Water is a fundamental solvent in biological systems, crucial for maintaining cellular functions and metabolic processes. In regenerative medicine, understanding the role of water as a medium for transporting nutrients and waste products is essential for cell survival, proliferation, and differentiation, particularly in tissue engineering approaches.

19) Field:
Field can refer to a specific area of study or practice within a broader domain, such as the field of regenerative medicine. This field focuses on developing innovative therapies, including the use of stem cells for tissue repair and regeneration, and addressing complex challenges like disease and tissue engineering applications.

20) Study (Studying):
A study refers to a systematic investigation aimed at discovering or validating knowledge. In the context of this document, the study evaluates the effectiveness of dental pulp stem cells for bone regeneration through innovative methods like the Rigenera Protocol, contributing to the broader understanding of regenerative strategies in dentistry.

21) Roman (Roma):
Roma (Rome) is the capital of Italy, a significant center for culture and healthcare advancements. In this context, the mention of Roma highlights a geographical connection to the study, offering a backdrop for research and clinical practices in dentistry, particularly regarding innovative techniques and pan-European collaborations in regenerative medicine.

22) Line:
Line often refers to boundaries or edges within anatomical contexts. In the surgical procedure described, the line denotes the enamel-cementum junction when separating the dental crown from the roots. Understanding these lines is crucial for precise surgical techniques that minimize trauma and ensure effective access to underlying structures in procedures.

23) Hand:
Hand generally refers to the manual dexterity and expertise of the surgeon performing procedures in dentistry. Skilled hand techniques are crucial for executing precise actions during surgeries, such as incisions, graft placements, and tissue manipulations, significantly influencing the outcomes of regenerative procedures involving dental pulp stem cells.

24) Post:
Post typically refers to actions taken after a procedure, such as post-surgical care and monitoring. In this context, post indicates the medications and follow-up protocols prescribed to ensure the patient recovers adequately, preventing complications and promoting successful healing after surgical interventions involving bone grafting and tissue regeneration.

25) Male (Mālē):
Male denotes the gender of the patient in the specific study. While gender can influence biological responses to treatments, understanding the implications of gender in regenerative medicine is essential for tailoring therapies effectively. The gender of the patient provides context for clinical considerations and potential variations in outcomes from treatments.

Other Health Sciences Concepts:

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Discover the significance of concepts within the article: ‘Sinus lift with autologous pulp micro-grafts: bone density case report’. Further sources in the context of Health Sciences might help you critically compare this page with similair documents:

Connective tissue, Statistical analysis, Surgical Procedure, Experimental protocol, Pearson's Chi Square test, Bone density, Tissue engineering, Bone regeneration, Bone grafting, Dental pulp stem cell, Collagen sponge, Atrophic maxilla, Therapeutical application, Digital Imaging and Communications in Medicine.

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