Beneficial Effect of Aqueous Stem Bark Extracts of Strychnos henningsii Gilg...

[Full title: Beneficial Effect of Aqueous Stem Bark Extracts of Strychnos henningsii Gilg in Streptozotocin-nicotinamide Induced Type 2 Diabetic Wistar Rats]

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Life International Journal of Pharmacology ISSN 1811-7775 Life science alert ansinet Asian Network for Scientific Information

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International Journal of Pharmacology 7 (7): 773-781. 2011 ISSN 1811-7775 DOI: 10.3923/ijp.2011.773.781 2011 Asian Network for Scientific Information Beneficial Effect of Aqueous Stein Bark Extracts of Strychnos henningsi Gilg in Streptozotocin-ricotinamide Induced Type 2 Diabetic Wistar Rats S.O. Oyedemi, G. Bradley and A.J. Afolayan School of Biological Sciences, University of Fort Hare, Alice 5700, South Africa Abstract: Strychnos hennings Gilg is commonly used in Southern Africa herbal medicines for the management of diabetes mellitus. The beneficial effect of the aqueous extract of S. henningsii at the dose of 125, 250 and 500 mg kg was investigated on some biochemical and hematological parameters in diabetic rats after 15 days of experimental periods. Significant decrease of blood glucose levels were found after oral administration of the extract at the dose of 250 mg kg(10.03 mmol L-) followed by 500 mg kg¹ (7.60 mmol L-) and then 125 mg kg (6.70 munol L ). The extract did not alter the levels of cholesterol, uric acid and kidney body weight ratio. In addition, there was no significant effect on the levels of basophils, monocytes and eosinophil bul appreciably increased White Blood Court (WBC), Tieutrophils and lymphocyte at. 500 mg kg¯¯. Moreover, the level of triglyceride, calcium, urea and liver body weight ratio was drastically reduced at certain doses. Treatment of diabetic rats with these extracts significantly reduced the activities of Aspartate Transaminase (AST) and Alamine Phosphatases (ALP). Whereas the levels of hemoglobin (Hb), Packed Cell Volume (PCV). Mean Concentration Volume (MCV), Red Blood Cell (RBC), Mean Concentration IIemoglobin (MCII), total protein, Alamine Aminotransferase (ALT), albumin and globulin were significantly increased as compared with diabetic rats. The present study indicated potent antidiabetic and antilipidemic properties of S. henningsii extract and its capability of regularizing some abnormalities associated with pathophysiologic condition of diabetes mellitus. Key words: Strychnos henningsii, diabetes mellitus, streptozotocin, nicotinamide, biochemical, hematological analysis, clinical significance INTRODUCTION Streptozotocin-nicotinamide is a method currently used to induce diabetes in animals that resemble non obese lype 2 Diabetes Mellitus (DM) in man (Nakamura et al., 2006). The induction of diabetic rats with Streptozotocin (STZ) increases the production of free radicals that damage the pancreatic DNA and thus affect. insulin secretion (Oguri et al., 2003). This is achieved by depleting Nicotinamide (NAD) which is a substrate of poly ADP ribose synthetase, an enzyme which involved in DNA repair. Pre-treatment of experimental animals with NAD allows minor damage to pancreatic SS cell (LeDoux et al., 1988). In diabetes, lipid abnormalities, anemia, alteration of liver and kidney functional indices have been implicated as major risk factors to the progression of both microvascular and macrovascular diabetic complications (Mandade and Sreenivas, 2011; Camargo and Gross, 2001). Several, antidiabetic drugs such as biguanide, sulphonylureas along with insulin have been employed for the treatment of this disease. Still none of these drugs was able to cure the disease without adverse reaction (Ances et al., 2007). There is a growing interest in the use of medicinal plants of low side effect for the management of diabetes mellitus especially in countries where access to conventional treatment of DM is inadequate (Rao and Rao, 2001; Pavana et al., 2008). Therefore, further scientific investigation is needed to give credence to the traditional usage of these plants in accordance to World Ilealth Organization (http://www.who.org). Strychnos henningsii Gilg (Logamiaceae) is commonly known as umnono in Zulu language and mostly cultivated in South Africa, Tanzania, Uganda and Kenya (Oyedemi et al., 2009). It is a small evergreen tree or shrub with leathery leaves and clean green-reddish stem. It is a free that grown up to 2-12 month in a dry or moist forest, wooded hillsides, coastal forest and stream banks. The finit is oblong and brown or orange when ripe. The bark is crown compact with dark green and glossy foliage. In local medicine of South Africa, the decoction or infusions of the stem bark is widely used for the Corresponding Author: A.J. Afolayan, School of Biological Sciences, University of Fort Harc, Alice 5700, South Africa Fax: 27866282295 773

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Int. J. Pharmacol., 7 (7): 773-781, 2011 management of DM (Oyedemi et al., 2009). It is also used in the eastern part of Africa for the treatment of various ailments including abdominal pain, syphilis, snake bite. gastrointestinal pain, rheumatism, diabetes, malaria and to hasten wound healing in animal (Oyedemi et al., 2009). About five compounds have been isolated including indolinic alkaloids, strychnine, brucine, curarine and bitter glycoside (Penelle et al., 2000). The plant has also been reported as potential agent in the development of new antinociceptive and antispasmodic dogs due to the presence of retuline-like alkaloids (Penelle et al., 2000). Our previous study on the phytochemical constituent. revealed the presence of flavonoids, tannins, saponins, proanthocyanidins. phenols and glycosides with significant values (Oyedemi et al. 2010a). Strong antioxidant and free radical scavenging activity observed was attributed to the presence of phenolics compounds indicating the ethnotherapeutic usage of this plant for the management of oxidative stress induced discases. Other studies conducted by Oyedemi et al. (2010b) revealed partial safety of sub-acute administration of aqueous stem bark extract of S. henningsii (SH) in rats due to negative effect on the normal functioning of white blood cells. Although the plant is used traditionally for the management of DM still need scientific data to give credence to its folkloric usage. A thorough search of the literature reveals that there is no information on the effect of this plan. 071 blood glucose levels and pathophysiologic symptoms in diabetic model. The present study was therefore designed to investigate the effects of the aqueous stem bark extract of SH on some biochemical and hematological parameters in STZ-NAD induced diabetic Wistar rats. MATERIALS AND METHODS Sample collection and extract preparation: The stem barks of SH was collected in February, 2009 from a thick forest in Amathole District (Eastern Cape, South Africa). The plant was identified by its vernacular name and later authenticated by Prof. DS. Grierson of Botany Department, University of Fort Hare. Voucher specimen (Sun MED 2005) was deposited at the Giffen Herbarium of the University. The bark material was cut into pieces and air-dried at room temperature in the laboratory. The dried material was then grounded into powder using an electric blender (Waring Products Division, Torrington, USA). About 10 g of the powdered plant material was extracted in 1000 mL of cold distilled water maintained on a mechanical shaker (Stuart Scientific Orbital Shaker, UK) for 48 h. The resulting solution was later filtered using a Buchner funnel and Whatman No.1 filter paper. The filtrate was quickly frozen at -10°C and dried for 18 h using a freeze dryer (Savant. Refrigerated vapor Trap: RV T11101, USA) to give a yield of 5.2 g of dry extract. The resulting extrael was reconstituted in distilled water to give desired doses of 125, 250 and 500 mg kg¯. Chemicals: The assay kits for urca, uric acid, calcium. total protein, total bilirubin albumin, cholesterol, triacylglycerol, alkaline phosphatase, lactate dehydrogenase, gamma glutaniyltransferase and alanine as well as aspartate aminotransferases were obtained from Randox Laboratories Limited, Ardmore, Co Antrim, UK. All other reagents used in this study were of analytical grade and were supplied by Merck Chemicals (Pty) Ltd.. Bellville, South Africa. Animals used: Male Wistar albino rats (Rattus novergicus) weighing between 125 and 255 g were obtained from the animal house of the Agricultural and Rural Development Research Institute, University of Fort Hare. The animals were kept in aluminum cages and maintained at a controlled temperature of 28°C with a 12 h light-dark cycle and humidity of 45-50%. They were fed with standard rat feed with water ad libitum for 15 days bul starved 12 h before the start of the experimenil. All animal experiments were conducted with NIII guidelines for care and use of laboratory animals which was approved by the animal ethics committee of the University of Fort Hare. Induction of type 2 DM in animals: The method described by Masiello et al. (1998) was adopted for the induction of type 2 diabetes in overnight fasted male Wistar rats. Animal grouping and extract administration: Thirty-six male rals were separated into six groups of six animals in cach group (30) diabetic surviving rats, 6 normal rats). The sex of experimental rats was chosen based on the availability of the animals. All the treatments were given to the animals orally using gavages. Group 1: Normal control rats administered drinking water daily for 15 days: Group 2: Diabetic animals received 0.5 mL of distilled water; Group 3-5: Diabetic rats treated daily with 0.5 mL of 125, 250 and 500 mg kg¹ body weight of SH extract. respectively and Group : Diabetic animals received 0.5 mL of glibenclamide (0.6 mg kg body weight) only. All the animals from each group were sacrificed by ether anesthesia 24 h after their respective 15 daily doses of the extract and distilled water. The serum was prepared by following the method described by Yakubu et al. (2005). 774

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Int. J. Pharmacol., 7 (7): 773-781, 2011 Haematological analysis: After animal anaesthesia with ether, blood samples were collected into sample bottles with EDTA to determine blood cell parameters including Red Blood Cells (RBC), Hemoglobin (IIb), Packed Cell Volume (PCV), Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin (MCII), Mean Corpuscular Hemoglobin Concentration (MCHC), Large Unstained Cell (LUC) and Red Cell Distribution Width (RCDW). The White Blood Cell (WBC) and other related indices such as basophils, monocytes, lymphocytes, neutrophils and eosinophils were also determined. All blood sample parameters were analyzed using hematology analyzer Coulter Horiba ABX 80 Diagnostics (ABX Pentra Montpellier, France). Serum biochemical evaluation: Serum samples were collected after centrifugation to determine the levels of Alanine (ALT) and Aspartate Aminotransferase (AST), Alkaline Phosphatases (ALP), wea uric acid total bilirubin, total protein, calcium, albumin, globulin, Triacylglycerol (TAG) and cholesterol in the animals by standard methods (Tietz et al., 1994). These parameters were estimated spectrophotometrically by using commercial kits obtained from Randox Laboratories Limited, Ardmore, Co Antrim, UK. Organ/Body weight of animals: The body weights of the animals were measured before the start and aller the experimental period (15 days). While the organ body weight ratio was determined after the animals were sacrificed. Statistical analysis: Dala were expressed as Means=SD of five replicates and were statistically analyzed using one way Analysis of Variance (ANOVA). Means were separated by the Duncan multiple test using SAS. Values were considered significaril al p=0.05. RESULTS The intraperitoneal injection of STZ-NAD resulted to significant increase (p<0.05) of plasma glucose level in comparison to normal rats as presented in Table 1. The normal rats were euglycemic (5.6 5.7 mmol L ) during the course of the study whereas the diabetic control rats were hyperglycemic (18.2-30.1 mmol L throughout the experimental period. After daily oral administration of plant extract or glibenclamide for 15 days, there was a significant reduction of blood glucose level. The blood glucose levels were reduced by 6.7, 10.03 and 7.6 mmol L al the doses of 125, 250 and 500 mg kg. respectively, while that of glibenclamide treated group was 8.98 mmol L-. The extract at 250 mg kg exhibited an effective reduction of blood glucose than glibenclamide treated group while the dose. 500 mg kg displayed a comparative effect. The beneficial effect of SH extract on red blood cells and its functional indices in diabetic rats is depicted in Table 2. The levels of RBC (4.5×10 L-1) and its differentials was markedly reduced in diabetic rats but increased significantly after oral administration of SII extract in a dose dependent mamer. The improvement on these parameters was comparable to that of glibenclamide and the normal control rats. The significant (p<0.05) decrease in the levels of total WBC and other related parameters in diabetic rats was shown in Table 3. The treatment of diabetic rats with SH extracts was parameter and dose selective. The levels of WBC (12.13±4.64), lymphocytes (8.51±4.64) and platelets (821 157.2) were notably increased at 500 mg kg 'followed by 125 and then 250 mg kg¯¯¯ in comparison with the normal control. All the three dosages of the SH extracts did not improve the levels of basophils, neutrophils, eosinophil and monocytes in comparison with diabetic. group. Table 4 shows the beneficial effect of SH extracts on kidney functional markers such as urea, uric acid and calcium of diabetic rats. The levels of urea, uric acid, calcium were 53.9, 0.4 and 2.6 mM, respectively in diabetic rals but significantly reduced after plant extrael. Treatment.. The extracts significantly reduced the concentration of urea but not dose related. The declined level of urea (26.90±0.01) aller extract administration at 125 mg kg 1 compared favorably well with glibenclamide treated group. Similarly, the level of calcium was markedly reduced at the doses of 250 (1.80±0.01) and 500 mg kg (2.00 -0.05) in comparison with normal and glibenclamide treated groups. Meanwhile, the extracts did not have any significant effect on uric acid at the three dosages investigated in this study. 1 A significant increase in serum cholesterol (6.7 mM) and triglycerides (2.2 mM) was observed in diabetic rats induced with STZ-NAD but decreased at certain dosages after administration of SH extracts for 15 days. The treatment of diabetic rals at the dose of 500 mg kg significantly reduced the level of cholesterol (4.6 mM) while other dosages did not show any promising effect. The three dosages reflected triglyceride lowering ability. However, the lowest and mid dosages showed a comparative effect with the normal control rats and glibenclamide treated group. Overall, the beneficial effect 775

[Find the meaning and references behind the names: Day, Micro, Hark, Ing, Jennings, Mcan, Henning]

Int. J. Pharmacol., 7 (7): 773-781, 2011 Table 1: Effect of oral administration of aqueous extract of' & henningsiz on plasma glucose level of STZ-NAD induced diabetic rats Plasma blood glucose (mmol L-') Treatment. Normal control Diabetic control Diabetic+SH (125 mg kg) Diabelic+SH (250 mg kg') Diabelic+SH (500 mg kg¹) Diabetic+glibenclamide (0.6 mg kg‍¹) 0 (day) 5.60 0.40 18.20-0.30 19.33-1.20** 24.30-0.09* 25.30-0.01* 22.50 3.30" 5 (day) 5.400.53 23.15±0.35 16.35±1.11** 22.20+0.08** 20.20+0.04* 19.30 +3.20" 10 (day) 5.60 0.30 15.53 1.30** 28.30-0.10 17.57-1.02* 15.28-0.10* 17.78=2.40" 15 (day) 5.700.32 30.10±0.10 12.56+0.90* 14.27+1.20* 17.70+0.30** 13.52+2.50" All values are Mean-SD of 6 rals in cach group. Significant different at *p<0.05 as compared with diabetic control group and significant different al **p<0.05 as compared with glibenclamide treated group. values in parenthesis denotes increase or reduction of blood glucose level in munol L Diabetic (111.9); 125mg kg (16.7); 250 mg kg (110.03); 500 mg kg (17.60); Glibenclamide (18.98) Table 2: The effect of aqueous extract of S henningsü bark on red blood cells and the differentials in STZ-NAD induced diabetic rats Strychnos henning sii bark extract (mg kg body weight) Parameters RBC (×102 L-¹) Control 8.94±0.04* 125 8.25-0.35 Hb (g dL) 15.03+0.06 15.80-0.84 PCV (IF) MCV (†1) 0.50+0.02 0.49-0.02 55.831.116 59.60-2.52 MCI (pg) MCHC g dL) RCDW (%) 16.930.35 30.27±0.82" 19.13 0.69 32.10-0.66 13.63±0.90° 11.55=3.13 LUC (*) 7.57+1.15* 0.05-0.04 250 8.761.23 500 8.57 0.19 Glibenclamide 8.15=0.40° Diabetes 15.90+1.97* 0.49+0.07 15.33-1.03 4.50±0.60- 8.43 +1.02 55.900.14 0.48-0.02 59.33-1.022 0.26 +0.036 40.1310.38 18.80 0.46 18.15.10.21 32.15±0.35% 16.65±2.17 0.04+0.03 16.03-0.32 0.48-0.01 55.90 1.15 18.70-0.26 33.10-0.35 15.10=1.77- 0.13-0.07 31.70-1.25 15.13-1.20 0.24-0.11 12.2010.40 22.23±0.837 9.13±0.55- 0.02+0.01b All values are Mean±SD of 6 rats in each group. Test values carrying superscripts different from the control down the column for each red blood and its differentials are significantly different (p<0.05) and those with the same superscript as the control are not significantly different Table 3: The effect of aqueous extract of Shenningsii bark on white blood cells and the differentials in STZ-NAD induced type 2 diabetic rats Strychnos jennings hark extract. (mg kg body weight.) Paranieters Control WBC (×10 1 17.00±3.20 125 10.8.5±2.03 250 7.72±3.723 Neurmphil (%) 5.37±1.17 0.13±0.016 0.76±0.30° Monocytes (%) 17.46±6.11* 0.98±0.41 1.47±0.016 500 12.13±1.0 1.72±0.30P 1.07±0.41 Glibenclamide 11.98 5.00% 1.01-0.1 3.04 0.64 Diabetes 7.33±0.93 0.59±0.51° 4.69±1.00 Lymphocyte (%) $5.40±6.86* &12±2.20 4.23±3.10 Eosinophil (%) 3.70+ 1.18 1.03+0.22° 1.20+0.60 8.51±3.04 1.07+0.60° 15.61-5.10 2.360.33 1.57-0.32 1.03+0.78 Basophils (%) 0.53+0.21° Platelets (x10°) 8.51.00 78.58 0.06+0.02 $55.00168.00 0.03+0.036 643.00-20.0 0.07+0,014 821.00 157.20 0.09-0.15 0.05+0.036 370.00 31.11 721.30-55.20 All values are Mcan-SD of 6 rats in cach group. Test values carrying superscripts different from the control down the column for cach white blood cells and its differentials are significantly different (p<0.05) and those with the same superscript as the control are not significantly different (p>0.05) Table 4: Effect of aqueous extract of & hennings bark on some renal function and lipid profiles parameters in STZ-NAD induced type 2 diabetic rats Group of animals Calcium (MM) Urea (IM) Normal Diabetes Diabeles+125 mg kg-1 2.2=0.03 2.6-0.01 1.1-0.01*** 26.9±0.53 53.9+0.05 26.9+0.01* Diabetes 250 mg kg-¹ 1.8 0.01" 45.3-0.06" Diabetes 500 mg kg 41.1-0.07 Glibenclamide 21.2±0.02* 2.0-0.05" 21=0.01* Uric acid (mM) 0.1±0.02 0.4+0.02 0.7+0.00 0.710.10 0.610.03 0.5±0.03 Cholesterol (IM) 1.1±0.09 6.7+0.02 6.7+0.01 7.410.02*** 4.6 10.05*** 6.2±0.01 Triglycerol (mM) 0.8±0.03 2.2+0.02 0.7+0.02* 0.710.04" 1.210.05" 0.50.01 * All values are Mean SD of 6 rats in each group. Significant different. at *p<0.05 as compared with diabetic control group and significant different at ***p<0.05 as compared with glibenclamide treated group Table 5: Effect of aqueous extract of Shermingsii bark on some liver functional indices in STZ- NAD induced diabetic rats Liver functional indices Group of animals Nonnal Diabetes 125 (ing kg) 250 (mg kg) 500 (mg kg) ALP (UL) 210.0±5.21 257.6+0.01* 253.5+0.00 252.10.02 AST (UL 165.1-1.91 ALT (UL) 59.5±3.77 Globulin (g LTotal protein (g L¹) Albumin (mM) TotalBilirubin (µM) 53.3±1.07 69.3 1.78 11.0±0.02 7.5±0.70 185.2-0.00 65.2+0.00 11.7+0.03* 55.7-0.02' 16.0+0.71° 44.0+0.05* 161.5-0.01 62.8+0.01* 30.8+0.05 92.1-0.00* 61.3+0.02 43.2+0.00 122.2=0.01 67.5±0.00 16.9±0.02 77.2-0.031 60.3±0.03 40.1±0.03 249.4±0.02bc 133.5=0.00 Glibenclamide 2:12.510.01 122.2-0.01 62.6±0.01° 58.710,000 10.0±0.07° 26.610.05 67.3=0.07 57.3±0.05 85.3-0.02 58.710.02 40.3± 0.01° 37.71001 All values are Mean SD of rats in each group. Test values carrying superscripts different from the control down the column for each liver functional indices are significantly different (p<0.05) and those with the same superscript as the control are not significantly different. (p>0.05) 776

[Find the meaning and references behind the names: Rapid, Change, Work, Ramesh, Noor, Fig, Better, Barik, Arun, Prior, Grover, Wal, Positive]

Weight of animals (g) 300Normal D2 Diabetic D3 ■D1 D4 250- 200- 150- 100- 50Int. J. Pharmacol., 7 (7): 773-781, 2011 0 0 5 10 15 No. of days Fig. 1: The effect of aqueous extract of S. henningsii on the body weight of Wistar rats over 15 days of treatment. Values are Mean±SD of 5 rats in each group. Dl: Animals fed with 125 mg kg of extract, D2: Animals fed with 250 mg kg of extract, D3: Animals fed with 500 mg kg body weight of extract, control: Animals receiving only distilled. Organ/body weight ratio (%) wal.er 14Kidney 12- Liver 10- 8Normal Diabetic Treatments D2 D3 Glibenc... Fig. 2: Organ body weight ratio over 15 days for different doses of the aqueous extract of S. henningsii in STZ-NAD induced diabetic rals. Values are Mean±SD of 5 rats in each group. D1: Animals fed with 125 mg kg of extract, 12: Animals fed with 250 mg kg of extract, D3: Animals fed with 500 mg kg body weight of extract, control: Animals receiving only distilled water. of plant extract at specific dosages was comparable with the positive control group treated with glibenclamide. The effect of SH extract on liver functions of diabetic rats is depicted in Table 5. The induction of diabetes in rats significantly (p<0.05) reduced the levels of total proteins, globulin and albumin while ALT, AST, ALP and total bilirubin were markedly increased when compared to control animals (Table 5). Treatment of diabetic rats with SH extracts at doses of 250 (40.1 10.03) and 500 mg kg' (10.3-0.01) remarkably reduced the level of total bilirubin whereas the dosage of 125 mg kg¯¹ (43.2±0.00) exhibited trivial changes on this parameter. Total protein, globulin and albumin levels were significantly (p<0.05) increased in diabetic rats after 15 days of treatment with SH extracts or glibenclamide. The increased levels of ALT, AST and ALP were reduced to near normal and compared favorably well with glibenclamide treated group. Figure shows the continuous reduction of body weight of diabetic rats. Oral administration of plant extract. at the three doses significantly improved the body weight (160-185 g) but not dose dependent as compared with normal or glibenclamide treated groups. Similarly, there was a change observed in organ/body weight ratio of diabetic rats (Fig. 2). The plant extract did not have any adverse effect on the kidney but the liver was slightly better (8-9) g) after 15 days treatment with SII extracts. DISCUSSION Streptozotocin acts as diabetogenic agent through the destruction of pancreatic SS cell which is correlated with a rapid reduction of NAD concentration. NAD Is a co-enzyme used as a specific inhibitor of streptozotocin during onset of diabetes mellitus (Grover et al., 2000). The prior intraperitoneal injection of NAD into rals was lo induce type 2 DM in animals by allowing minor damage to pancreatic SS cell. The results obtained from this study after treatments of diabetic rats with SH extracts are very much promising with equivalent effect to that of glibenclamide. The plant extract significantly reduced the blood glucose level at the three doses but not dose related. The dose at 250 mg kg showed a potent hypoglycemic activity in comparison to normal or glibenclamide treated group. Similar observation was reported on lehnocarpus frutescens in animals induced. with streptozotocin-nicotinamide (Barik et al., 2008). Though the probable mechanism of action of this plant was not investigated but could be linked to prevention of death or restoration of partially destroyed pancreatic SS cells or increased uptake of glucose (Noor et al., 2008). Moreover, the antioxidant and free radical scavenging properties of this plant as shown in our previous work might increase the resistant of SS cells to the toxic effect of STZ by activating antioxidant enzymes (Oyedemi et al., 2010a). The presence of phenolics compounds as previously reported in our studies may induce insulin secretion in diabetic animal models in accordance to other several studies (Al-Awwadi et al., 2004; Arun and Ramesh, 2002). 777

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Int. J. Pharmacol., 7 (7): 773-781, 2011 Hematological parameters have been commonly used in the diagnosis of various diseases and pathological condition of foreign compounds including plant extracts, drugs, dyes and several others on the blood constituents of animals (Oyedemi et al., 2010b). In this study, the observed decreased levels of red blood cells, Hb, MCV, MCH, MCHC, RCDW and PCV in chabetic rals gives an indication of anemic condition. The decreased level of IIb concentration has been attributed to impairment on the rate of RBC formation as a result may have negative effect on the oxygen carrying capacity of the animals. This observation corroborated with the report. of Baskar et al. (2006) but opposed the findings of Junod et al. (1969) who reported probable loss of diabetogenic action of STZ due to prior administration of NAD. This observation provided evidence that intraperitoneal injection of nicotinamide into the rats may not completely exterminate the effect of STZ on pancreatic SS cells but allow minor damage by repairing poly ADP ribose synthelase (LeDoux et al., 1988). The disturbed hematological alteration of red blood cells and its related indices in diabetic animals was improved upon plant extrael treatment by preventing hemolysis of erythrocytes caused by lipid peroxidation through its antioxidant property (Oyedemi et al., 2010a). The decreased level of total WBC and its functional indices in STZ induced diabetes concurred with the report of Paul (2002) on the suppression of immune system caused by damaged WBC (Table 3). The reduction of these parameters may have a significant effect against the animals by exposing them to pathogenic infections (Adedapo et al., 2007). Oral administration of SII extract for 15 days notably increase the level of WBC, lymphocytes and platelets at the dose of 500 mg kg. Meanwhile, there was no significant effect (p>0.05) on the levels of monocytes, basophils and eosinophils in comparison to normal and glibenclamide treated groups. This finding was contrary to the report of Oyedemi et al. (2011), who found that administration of A. africana into diabetic rats induced with STZ had potential to improve the WBC functional indices. The significant increase of lymphocyte levels reflects possible immunomodulatory effects of S11 extract in diabetic rats. The data obtained on the white blood counts and platelets indicated that SH extract did not have strong potential to boost the immune and defense system of diabetic animals but could improve the anticoagulation during severe bleeding (Dahlback, 2008). It could be inferred from this study that S. henningsii may contain some bioactive compounds with ability to improve the impaired production of WBC by stimulating maturation of committed stem cells responsible for WBC production. The abnormal changes observed on the nephropathic markers such as urea and uric acid of diabetic rats reflect the diminish ability of kidneys to filter waste products from the blood as a result of disruption in filtration and electrolyte balance (Atangwho et al, 2007). The continued administration of plant extract for 15 days into diabetic rats provide corrective measures on the kidney which is reflected on the drastic reduction of urea and calcium at certain dosages. Similar observation was reported on the aerial parts of Silybum marianum (Pakistan plant) against nephropathy in STZ diabetic rats (Vessal et al., 2010). Though the histology is nol. evaluated in this study, however, the data obtained on the kidney body weight ratio suggested that the extract could lessen hypertrophy an indices of nephropathy. This result. also implies that SII extract may have the potential to reverse the kidney functional status impaired in diabetic rats and to prevent diabetes related complications as shown in this study. High levels of lipids such as Iriglycerides and cholesterol have been observed in diabetes subject due to lack or deficiency of insulin (Anreddy et al., 2010). A significant increase in serum cholesterol and triglycerides was observed in diabetic rats induced with STZ-NAD which agrees with the finding of Burcelain et al. (1995). This abnormality may therefore be regarded as consequence of increase mobilization of free fatty acids from peripheral fat depots and may have a greater risk for the development of coronary heart diseases (Bopanna et al., 1997). In this study, the level of scrum cholesterol and Iriacylglycerol in diabetic rats was significantly reduced at certain doses in comparison with normal or glibenclamide treated groups (Table 4). Karim et al. (2011) reported similar results on A. cepa, A. sativum and C. roseus for their successful serum lipid control in diabetic animal model. The lipid lowering ability of this plant justified its glycemic control and thus could be of great advantage for the management of DM and the complications associated with atherosclerosis or coronary heart attack (Ghasi et al., 2000). The data obtained from this study showed the decreased levels of total protein, globulin and albumin in diabetic rats and this is consistent with the result oblained by Bakris (1997) and Tuvemo et al. (1997). Similarly, the levels of total bilirubin, ALT, AST and ALP were significantly increased in DM which indicates hepatic dysfunction as reported with other investigators (Irshaid and Mansi, 2009, Al-Attar and Zari, 2007). The increased protein catabolism and urca formation that are seen in diabetic rats could be accountable for the elevation of these tissues transaminases. In this study, administration of SH extracts caused significant (p<0.05) 778

[Find the meaning and references behind the names: Rubia, Pharm, Vet, Gain, Obi, Punjab, Wine, Rii, Ebong, Govan, Anees, Joshi, Goyal, Sci, Chem, Bahauddin, Jain, Leaf, Hashmi, Tripathi, Ginger, Root, Flatt, Mbeki, Linn, Patient, Abatan, Abo, Karuna, Mandi, Manner, Reddy, Eyong, Holy, Jawad, Bharathi, Pak]

Int. J. Pharmacol., 7 (7): 773-781, 2011 increase in the biosynthesis of total proteins, globulin and albumin as compared with nommal group. There was a significant restoration of total bilirubin after SII treatment which indicated the stabilization of plasma membrane and protection of liver cells. In addition, the damage caused by STZ on the liver cells was substantiated by the elevated level of serum ALT. ALP and AST. The increased that might be due to leakages from the cytosol or changes in the permeability of liver membrane. In this investigation, the levels of ALP, ALT and AST were reduced in a dose related manner as compared with diabetic animals thus improving renal and hepatic functions. This observation concurred with the report of Iwalokun et al. (2006), who reported hepatoprotective potentials of leaf extracts of V. amygdalina in mice. A significant decrease in the body weights is widely associated with diabetes patient due to loss of appetite which was also confirmed in this study. Similar observation was reported by Safiyeh et al. (2007) on STZ induced diabetic rats. The effect of SH extracts on the body weight of diabetic rats was observed after 15 days of treatment. Oral administration of plant extract appreciably increased the body weight gain of the animals near that of normal rats but not dose related (Fig. 1). The observed result signified the ability of SH extract to manage glucose properly as well as control muscle wasting and induced adipogenesis (Swanston-Flatt et al., 1990). The changes in relative organ holy weights ratio in the diseased animals are generally established as useful indicators of tissue damage (Fig. 2). The extract clicited slight effect on the liver while there was no significant. effect on the kidney which may be due to lack of direct organ toxicity as reported by Oyedemi et al. (2010a). CONCLUSION In conclusion, the intraperitoneal injection of STZ-NAD induces type 2 diabetes mellitus with instability of some biochemical and hematological parameters. The data obtained from this study specified the reversible of anemic condition by SH extract in diabetic subject. It was further shown that plant extract can ameliorate or prevent secondary complications such as nephropathy and hepatic damage during chronic hyperglycemia. In addition, the plant extract has definitely shown to improve anticoagulant effect during the progression of diabetes. However, the extract did not have any prominent effect on the immune system of the animals. The beneficial effect of aqueous stem bark extract of S. henningsii as shown in this study was parameters and dose selective. Further studies are underway to investigate the pharmacological and mechanism of action of this plant in diabetic model. ACKNOWLEDGMENT The authors are thankful to the Govan Mbeki Research and Development. Center, (GMRDC) of the University of Fort Hare, Alice, South Africa for the financial support. REFERENCES Adedapo, A.A., MO. Abatan and 0.0. Olorunsogo, 2007. Effects of some plants of the spurge family on haematological and biochemical parameters in rats. Vet. Arhiv, 77: 29-38. Al-Attar, A.M. and T.A. Zari, 2007. Modulatory effects of ginger an clove oils QT1 physiological responses in streptozotocin-induced diabetic rats. Int. J. Pharmacol., 3: 34-40. Al-Awwadi, N., J. Azay, P. Poucheret, G. Cassanas and M. Krosniak et al., 2004. Antidiabetic activity of red wine polyphenolic extract, ethanol or both in streptozotocin-treated rats. J. Agric. Food Chem... 52: 1008-1016. Anees, M., A. Jawad and I. Hashmi, 2007. Distribution of ABO and RII blood group alleles in Mandi Bahauddin District of Punjab, Pakistan. Proc. Pak. Acad. Sci., 11: 289-291. Anreddy, R.N.R., M. Porika, N.R. Yellu and R.K. Devarakonda 2010. Hypoglycemic and hypolipidemic activities of Trianthema portulacastrum Linn. plant in normal and alloxan induced diabetic rats. Int. J. Pharmacol., 6: 129-133. Arun, G.S. and K.G. Ramesh, 2002. Improvement of insulin sensitivity by perindopril in spontaneously hypertensive and streptozotocin-diabetic rats. Indian J. Pharmacol., 34: 156-164. Alangwho, LJ. P.E. Ebong, M.U. Eleng, E.II. Eyong and AU. Obi, 2007. Effect of Vernonia amygdalina Del leaf on kidney function of diabetic rats. Int. J. Pharmacol., 3: 143-148. Bakris, G.L., 1997. Diabetic nephropathy. What you need to know to preserve kidney function? Postgrad. Med.. 93: 89-100. extract of Barik, R., S. Jain, D. Qwatra, A. Joshi, G.S. Tripathi and R. Goyal, 2008. Antidiabetic activity of aqueous rool Ichnocarpus frutescens in streptozotocin-nicotinamide induced type-II diabetes in rals. Indian J. Pharmacol., 40: 19-22. Baskar, R., L.M. Bhakshu, G.V. Bharathi, S.S. Reddy, R. Karuna G.K. Reddy and D. Saralakumari, 2006. Antihyperglycemic activity of aqueous root extract of Rubia cordifolia in streptozotocin-induced diabetic rats. Pharm. Biol., 44: 475-479. 779

[Find the meaning and references behind the names: Rathod, Rathi, Yano, Vijayalakshmi, Maury, Seed, Akinwande, Akinpelu, Aloe, Hall, Vera, Clin, Sattar, Kannan, Alabi, Vats, Forbes, Neem, Patton, Roye, Topic, Vivo, Assan, Diet, Christen, Manoharan, Lam, Renold, Smith, Ofili, Pac, Nouman, Girard, Wilson, Role, Hashimoto, Endo, Ogata, Rather, Eugenia, Manickam, Nat, Nwobodo, Kande, Ono, Adewusi, Brandt, Balaraman, Lambert]

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