Excretion of Levofloxacin into Saliva in Renal Failure Rat Model

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Life International Journal of Pharmacology ISSN 1811-7775 Life science alert ansinet Asian Network for Scientific Information

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International Journal of Pharmacology 6 (6): 944-949, 2010 ISSN 1811-7775 2010 Asian Network for Scientific Information Excretion of Levofloxacin into Saliva in Renal Failure Rat Model IN.W. Sheikh, 'A.S. Tripathi, 'V. Chitra, 'A. Choudhury and S.P. Dewani 'SRM College of Pharmacy, Kathankulathur, Chennai, India Institute of Pharmaceutical Education and Research, Hinganghat Road, Wardha, India Abstract: To clarify effects of renal failure on salivary distribution of Levofloxacin (LOFLX), a quinolone antibiotics, blood and saliva were collected from the double-step 5/6th-nephrectomized and sham-operated (control) rats after bolus iv., administration of Levofloxacin (10 mg kg ). The concentrations of Levofloxacin in these samples were determined by high-performance liquid chromatography. Renal failure induced by the partial nephrectomy significantly elevated plasma levels and cumulative salivary excretion of Levofloxacin when compared to control rats. Total body clearance was significantly decreased by the renal failure, although salivary clearance of the partially nephrectomized rats was larger than that of the control. The saliva/plasma concentration ratios in the rats with renal failure were significantly greater than those in the control rats. The results of this study suggest that the salivary excretion of Levofloxacin is significantly increased by renal failure. Key words: Levofloxacin, Renal failure rat model, therapeutic drug monitoring INTRODUCTION Levofloxacin is a synthetic fluoroquinolone antimicrobial agent with potent and broad activities against both gram-negative and gram-positive pathogens (Garrison, 2003) and is widely prescribed as the drug of choice to treat various infectious diseases. New quinolone antibiotics including LOFIX have been known to have severe adverse effects such as seizures and rhabdomyolysis. It has been reported that LOFLX undergoes a limited metabolism and is primarily excreted by kidney mainly as active drug, inactive metabolites (N-oxide and demethyl metabolites) represent less than 5% of the total dose (Albarellos et al., 2005). Its clearance is approximately 9L hand its half life is approximately 7 h in healthy subjects, in renal failure LOFLX clearance is reduced by approximately 75% and half life is prolong to 35 h (Czock et al., 2006). Additionally, many investigators have reported the pharmacokinetic interaction that gastrointestinal absorption of new quinolones is inhibited Or reduced by antacids (Johnson et al., 1988). Therefore, LOFLX may be a quinolone dosage adjustment of which is required on the basis of the therapeutic drug monitoring. Drug monitoring using the saliva offers a convenient and noninvasive alternative to blood analysis with particular advantages in geriatric and pediatric cases. For several drugs, it was reported that determination of saliva levels was successfully used for the therapeutic drug monitoring. It has been reported that. The lipophilicity of the fluoroquinolones primarily determines the extent of salivary excretion (Langlois et al., 2005) and LOFLX has intermediate lipophilicity (Andersson and MacGowan, 2003) and has been demonstrated to show extensive distribution and good penetration into the extravascular compartment. It has been suggested that saliva might be substituted for plasma in therapeutic drug monitoring (Zuidema et al., 1996). This study was conducted to clarify effects of renal failure on salivary distribution of Levofloxacin (LOFLX). MATERIALS AND METHODS Chemicals: LOFLX was kindly supplied by Micro Labs. Lld. Pondicherry (India). All other reagents and solvents were commercially available and of analytical grade. Animals: Ten to twelve-week-old male rats albino (wistar) weighing 300 to 350 g procured from King Institute, Guindy, Chennai (India) were used in this study. Animal Ethical Committee approved experimental protocol under guidelines of CPCSEA (662/02/C/CPSCEA, July 2002), New Delhi. India and Institutional Animal Ethical Committee (Approval RefNo. IAEC/27/2007). Animal were housed in a laboratory maintained a 12 h light-dark cycle and controlled room temperature (23±2°C) and relative humidity (50±10%). The animal received an appropriate diet (rat chow). A restricted feeding procedure was used Corresponding Author: Naheed Wascom Sheikh, 28, Kohinoor Colony, Wani Road, Yavatmal-445 001 (M.S.), India. Tel: +917232241250, +919974756353, +919403937585 944

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Int. J. Pharmacol., 6 (6): 944-949, 2010 for at least 1 week before the study 15 g of feed was given per day at noon. This ensured overnight fasting for 14-16 h before surgical procedure. The project was conducted at SRM college of Pharmacy, SRM University Chennai. India, form 06/12/2007 to 06/03/2008. Preparation of chronic renal failure model: The rat. was anaesthetized with i.p. injection of pentobarbital (40-50 mg kg) (Feinberg and Campbell, 1997). On the first day the left kidney was 2/3 nephrectomized and the eight day the right kidney of the same rat was nephrectomized (Perez-Ruiz et al., 2006). The sham-operation (only incision and sutures on the abdomen) was performed on the controls. 011 The body weight, concentrations of plasma Creatinine and blood urea nitrogen in the sham-operated and 5/6th-nephrectomized rats were measured by using autoanalizer. Pharmacokinetic study: The rat was anaesthetized with i.p. injection of pentobarbital (40-50 mg kg). After tracheotomy and catheterization, the right jugular vein was canmulated with a silicon polymer Tubing (T.D. 1.0 mm, O.D. 1.5 mm) for bolus administration of LOFLX and for collection of blood samples. Then the femoral vein was cannulated with a polyethylene tubing (PE-50; I.D. 0.58 mm, O.D. 0.965 mm) for constant-rate infusion of Pilocarpine hydrochloride (9 mg/h/kg) to stimulate salivation Throughout the experiments, the body temperature of rats was maintained at 37.5°C using a heated pad. The rat receive the constant-rate infusion of pilocarpine for 2 h to stabilize the salivation (Garcia-Olmos et al., 2003), the rats received a bolus i.v., injection of LOFLX at a dose of 10 mg kg-1. Blood samples were collected by jugular vein (Thrivikraman et al., 2002) just before drug administration (about 200 il) and at designated times of 3, 5, 10, 20, 30, 40, 60, 80, 100, 120 and 140 min (about 100 uT) after administration and the plasma was immediately separated by centrifugation after heparinization. Saliva samples were separately collected at consecutive 10 or 20 min periods (0 to 10, 10 to 30, 30 to 50, 50 to 70, 70 to 90, 90 to 110, 110 lo 130 and 130 to 150 min) aller drug administration. Parotid and mendibular mixed saliva samples were collected by using dental cotton roll. The salivate is used to absorb the saliva into a dental cotton roll. Dental cotton roll after being socked with saliva, the container fits into a polystyrol tube that is then centrifuged for 3 min at about 1000 rpm. During centrifugation the saliva passes from the cotton roll into the lower part of the tube. The container is then taken out of the tube and the clear saliva is poured out of the tube. Cellular particles are retained at the bottom of the tube in a small sink compartment.. The pH of plasma and saliva: To determine the pH of plasma and saliva of the sham-operated and nephrectomized rats, the other rats received the surgical operation and saliva stimulation in the same manner as for the pharmacokinetic study. Saliva sample were separately collected under a liquid paraffin layer (about 0.15 mL) in a microtube during consecutive two 75 min periods from 2 h after the beginning of constant-rate infusion of pilocarpine. Blood samples were collected immediately before the saliva collection and midway through the collection period and the plasma were obtained by centrifugation after heparinization. Immediately after the collection of plasma and saliva, the pH of these samples was determined by a compact pII meter. Sample extraction procedure: To 0.2 mL of heparinized plasma, 0.2 mL. methanol was added. Plasma proteins were precipitated by shaking in an ultrasonic bath followed by centrifugation for 10 min at 2000 rpm. The supernatant was evaporate and reconstituted with mobile phase. Drug analysis: Reverse phase high-pressure liquid chromatography (RP-HPLC) method was used for the calibration and determination of LOFLX in plasma and saliva, with column 5 μm; pump model LC-10 ATVP, Shimadzu, Japan; ultraviolet detector model SPD-10 A VP. Shimadzu Japan was used to determine the concentrations of LOFLX in plasma and saliva of rat. (Santoro et al., 2006) the mobile phase was acetonitrile: water (80:20, v/v) stationary phase was Phenomenex Luna C. Column (250×4.6 mm). The pH was adjusted to 3.5 by ortho phosphoric acid. The flow rate was 1.1 mL min and the sample was determined at. 296 nni. Data analysis: Measured plasma concentration (C) time (t) data for LOFLX were analyzed on the basis of a two-compartment model expressed as the following equation: CA. c+B. cft where, A, B. a. b are hybrid parameters. The nonlinear least-squares regression program WinNonlin was used for the regression analysis to obtain the hybrid parameters and secondary parameters, ie, the elimination half-life (ta) total body clearance (CL) and volume of distribution at the steady state (Vd). The flow rate of 945

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Int. J. Pharmacol., 6 (6): 944-949, 2010 saliva was determined gravimetrically assuming the specific gravity to be approximately 1.0. The S/P ratio of LOFLX was calculated as follows: ■ • . " The lag time for saliva collection was calculated from the salivary flow rate and the internal volume of saliva carmula The true midpoint of the saliva collection period was presumed from the lag time The plasma LOFLX concentration at the true midpoint was predicted by the two compartment model equation for the each ral. The S/P ratio was expressed as the measured saliva concentration divided by the predicted plasma concentration The salivary clearance (CL) of LOFLX was calculated by multiplying the S/P ratio by the total salivary flow rate (per body weight) into which the measured flow rate of the single side was doubled assuming that salivary LOFLX concentrations and salivary flow rates in the both sides would be equal. Statistical analysis: The results were expressed as the Mean SD for the indicated numbers of experiments. The significance of differences between the mean observations for two groups was determined using Studen's -lest. Repeated measures analysis of variance was used to test for differences in the plasma concentration-time profiles between the treatments. RESULTS Levofloxacin assay: Calibration curves for LOFLX in the plasma and saliva were satisfactorily linear over the concentration ranges from 1 to 16 and 1 to 16 µg mI, '. shown in the Table 1 and 2, respectively. The coefficients of variation for the assay were 2.1.5% at 2 μg mL of the plasma concentration and 3.32% al. 4 μg mL of the saliva concentration. The respective regression equations for plasma and saliva were y-238950.2 x 5607.514 (r 0.999) y=276625.2x-25097.29 (r = 0.999) where, y is the peak-area of the drug to the internal standard, x is the concentration in plasma or saliva (mgm) and is the coefficient of correlation. The limits of determination were established at 1 μg mL in plasma and at 1 μg mL in saliva. Blank plasma and saliva samples did not interfere with the peaks for LOFLX. Table 1: Linearity values for LOTLX in plasma by RP-HPLC Method Concentration (µg mL¯) 0 1 2 4 8 16 Peak area Û 280900 460934 956672 1906891 3835704 Table 2: Linearity values for LOFLX in saliva by RP-HPLC method Concentration jug mL¯ ) 0 1 2 4 8 16 Peak area 0 288739 577478 1143582 22961 83 4419982 Table 3: Body weight and biochemical data of sham-operated and nephrectomized rats Measurement Sham-operated 332.50+5.57 33866±5.31 0.44±0.039 35.40±7.31 Nephrectomized* 341.01±7.96 330.33±5.30 0.85±0.074 135.33±15.38 Body weight before treatment (g) Body weight After treatment (g) Plasma Creatinine (ing dL)* Blood urea nitrogen (ing L-')* "Data are expressed as the Mean SD. There are significant difference from sham-operated rats (student's t-test *p<0.0001) Pathophysiological data: The boxly weight. concentrations of plasma Creatinine and blood wea nitrogen in the sham-operated and 5/6th-nephrectomized rals are summarized in Table 3. Plasma Creatinine levels were less than the detection limit of 0.5 mg dLin sham-operated rats, whereas nephrectomized rals had higher Creatinine levels of about 0.85±0.071 mg dL¯¹. Plasma urea nitrogen levels in the nephrectomized rats were about four times as high as those in the sham-operated rats. Plasma and saliva pII: The pII of plasma obtained from the sham-operated and nephrectomized rats ranged from 7.2 to 7.7 throughout the experiment. Mixed saliva had the pH of 7.8 and 8.1. respectively, in both pretreatment groups. In the plasma and saliva pH, there was no difference between the sham-operated and nephrectomized groups and between the initial and latter halves of the sampling periods. Plasma concentration-time profiles of LOFLX: In the nephrectomized rals, higher plasma LOFLX concentrations were observed when compared to the sham-operated rats shown in Table 4. In both groups. plasma concentrations of LOFLX were found to decline biexponentially with time. Two-compartment model was the most adequate to describe the time courses on the basis of the minimum AIC estimation. The corresponding pharmacokinetic 946

[Find the meaning and references behind the names: Top, Range, Fig, Six, Tri, Cach, Matin, Point, Tinie, Mcan, Free, Non]

Int. J. Pharmacol., 6 (6): 944-949, 2010 Table 1: Geometric means/geometric SD (range) of LOFLX in plasma were tabulated against different time interval Nephrectomized" Tinie (min) Sham-operated" -(μg mL. )- 3 7.24±0.0547 ཇ 6.60±0.0509 10 5.62100113 20 3.98±0.0307 30 3.31+0.0250 10 2.69±0.0213 60 2.29 0.0213 80 1.94 10.0178 9.12-0.0865 8.34 0.0789 6.95 0.0659 4.99 0.0468 4.04-0.0377 3.15 0.0313 2.82 0.0258 2.40-0.0236 100 1.69+0.0172 2.11-0.0194 120 1.54±0.0172 1.99=0.0175 1.10 1.1110.0132 1.83 0.0167 Table 7: Measured and predicted SP ratio of LOLX after balus i.v., administration to sham-operated and nephrectomized rats Measured' Predicted Sham-operated Nephrectomized Sham-operated Nephrectomized 1.25-0.026 1.21 1.36 Saliva 0.99±0.045 *Data expressed as the Mcan+SD. The number of rats is indicated in the parentheses. They are based on the mean of the values obtained from 90 to 150 min after drug administration in individual rats. There are significant differences timom sham-operated rats (student's t-test: p>0.05). They are predicted with the equation: S/1 ratio = [(1+10) ]/[(1+10)] non 1.5Sham-operated Nephrectomized Table 5: Pharmacokinetic parameters of LOFLX after i.v., bolus administration to sham operated and nephrectomized rats Parameter Sham-operated' A ug mil. ¹ 5.13±0.0891 B (μg mL-') 2.5810.1050 (102 min) 5.68+0.1235 3 (10 min) The (min) 4.2940.2131 161.98+8.2460 CL (mL/min/kg) 12.5110.2671 1.0Nephrectomized 7.010.1577 0.5- 3.03.10.0308 5.73+0.0468 0.0 3.73+0.1160 10-30 30-50 50-70 185.90±5.938 70-90 90-110 110-130 130-150 Time (min) 9.37810.2836 V₁ (mL. kg¹) 2922.1099.517 2513.83 14.308 A, B, and P: Hybride parameters: t: Elimination half life: C: Total body clearance V: distribution volume steady state. Data are expressed as the Mcan=SD of six rate. There are difference two groups Table 6: Cumulative excretion of LOFLX into saliva after iv., bolust administration to sham-operated and nephrectomized rats Time (min) 0-10 10-30 30-50 50-70 70-90 90-110 110-130 130-150 Sham-operated' 0.0260 0.0039 0.0360+0.0047 0.0443±0.0038 0.0520±0.0037 0.0602L0.0033 0.066710.0034 0.0715+0.0040 0.0768±0.0036 (ugmLNephrectomized 0.029410.0041 0.0472+0.0049 0.0593±0.0048 0.0690±0.0038 0.078110.0031 0.085410.0043 0.0914+0.0033 0.0969±0.0044 parameters of LOFLX are summarized in Table 5. The CL... was significantly decreased to about 25% by the partial nephrectomy and the top of the nephrectomized rats tended to be longer than that of sham-operated control. It was observed that V, of sham-operated was higher as compare to nephrectomized rats. Salivary excretion: In sham operated rats, the cumulative salivary excretion of LOFLX up to 1.50 min aller administration was 0.0768% of the dose Table 6. The nephrectomy induces a significant increase in the cumulative salivary excretion of LOFLX in saliva. The salivary flow rates and CT of LOFLX were calculated from the mean observations of three collection periods from 90 to 150 min after administration and were compared between two groups of the rats. The salivary flow rate in the sham-operated rats was 18.2611.2 µL/min/kg. The nephrectomy tended to increase the flow rates of saliva to 22.19±1.5 μL/min/kg, respectively, although there was significant difference between sham-operated and nephrectomized rats. 947 to Fig. 1: S/P Ratio of LOFLX after bolus administration sham-operated and nephrectomized rats. Each point represents the Mean and SD of six rats. There are a significant difference of excretion between sham-operated cumulative and nephrectomized group (student's -lesl p<0.0001) The CL of LOFLX was 27.73 and 18.07 µL/min/kg for nephrectomized and sham operated rats saliva. The S/P ratios of LOFLX for each saliva are shown in Fig. 1. Tri cach group, the S/P ratios were nearly constant during the periods, which corresponded to the elimination phase of the plasma concentration-time profile. The S/P ratios in the nephrectomized rats were slightly higher than those in the sham-operated control during 70 to 150 min. after administration. Prediction of S/P ratio by Matin's equation: In as much as LOFLX is weakly acidic, the following equation may predict the saliva to plasma concentration ratio. 1-10 (pK,-pH,)xf, 1110 (pk-pxf, where, pH, and pH are pH values of saliva and plasma. respectively; f, and f, are free fraction of total drug concentration in saliva and plasma, respectively. Table 7 summarized the observed and predicted S/P ratio for sham-operated and nephrectomized rats in saliva samples according to the above equation

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DISCUSSION Int. J. Pharmacol., 6 (6): 944-949, 2010 for The Lise of saliva instead of blood pharmacokinetic investigations has obvious practical advantages. It is a painless, non-invasive procedure, hence suitable for the collection of multiple specimens. Variable results have been reported for the ratio of saliva to serum concentrations (Edelstein et al., 1996). The presenil study shows Thal several pharmacokinetic parameters can be calculated using salivary concentrations of LOFLX as can be done with plasma. The estimation of LOFLX levels in saliva seems to be useful in therapeutic drug monitoring and pharmacokinetic studies. Lot of methods are available for producing renal failure in experimental animals, which include the administration of various nephrotoxic agents such as uranyl nitrate, mercurials, cisplatin, and ureteral ligation partial nephrectomy. Although the chemical methods. such as uranyl nitrate injection are simple and reliable (Giacomini et al., 1981) at the nephrotoxic agents could also damage salivary glands. Therefore, in this experiment. the double-step 5/6th-nephrectomy was used to incluce the experimental renal failure. This surgical method can damage only the kidneys, so that it is possible to investigate the effects of renal failure per se on salivary excretion of the drug. Plasma creatinine, blood urea nitrogen levels were remarkably raised by the partial nephrectomy, indicating that severe renal failure was produced The saliva. flow rale in the rals with renal failure tended to be larger than those of the control, impairment of saliva secretion was not induced by renal failure in rats. The elevated secretion of saliva may be in compensation for decreased urine production. Based on the salivary levels, the present slukly compares the pharmacokinetic parameters of LOFLX in sham operated rat and in 5/6 nephrectomized rat model. The pharmacokinetic parameters of LOFLX such as ty CLT and Vd, does change when given in 5/6th nephrectomized rat model. Suggest that the pharmacokinetics of LOFLX get affected or altered when administered chronic renal failure rat model. Salivary excretion and S/P ratio of LOFLX in saliva were significantly higher than those in control rats. Generally, it is known that the S/P ratio of weakly acidic or basic drugs depends on the salivary pII. As for quinolone antibiotics, it was reported that salivary penetration of Levofloxacin was pH-dependent and higher saliva levels of Levofloxacin were found in the more acidic samples. Therefore, in this study, a theoretical S/P ratio of LOFLX were calculated according to the pH partition theory and compared to the measured ratios. Furthermore, the measured S/P ratios were less than the values predicted 948 according to the pH-partition theory where the zwitterionic species were assumed to predominantly penetrate into salivary glands by passive diffusion. Thus, LOFLX distribution into saliva could not be apparently explained by the pII-partition theory itself. In this theory, it is assumed that a nonionized (or zwitterionized) species is sufficiently lipophilic and rapidly diffuse across the membrane. If a zwitterionic form of LOFLX was much less lipophilic than the uncharged form, lower S/P ratios compared with the theoretical values would not be unexpected. Another explanation for the discrepancy between measured and theoretical S/P ratios can be discussed. Passive diffusion through the membrane may not be only the mechanism for LOFLX penetration into saliva. Possible active transport system, which could pump out this quinolone from saliva to the circulation. may operate. It has been known that there are specific transport. systems in the salivary glands (Novak, 1990). The transport systems could actively carry not only endogenous substrates but also exogenous materials including various drugs through the salivary gland epithelium membranes. Recently, it has been reported that the specific transport systems for quinolone antibacterial agents including LOFLX exist in the epithelium membranes of rat choroid plexus (Soriano and Rodriguez-Cerrato, 2002). Since, salivary glands also have the epithelial membrane, which is morphologically similar to choroidal and renal tubular epithelium, it is likely that a specific system for LOFLX may function in the salivary glands and LOFLX may be reabsorbed from saliva via this system. Relatively low salivary distribution of this quinolone in rats may be due to this putative efflux mechanism, which could transport LOFLX from saliva to blood. Interesting results were obtained concerning the effect of nephrectomy on salivary excretion of LOFLX. The measured S/P ratios in the group of renal failure were greater than the control saliva, suggesting that LOFLX distribution between the blood and saliva may be allered in the rals with renal failure (Tsagamosa et al., 2008). In contrast, it was reported that saliva and plasma levels of procainamide, a basic drug, were higher in renal-impaired rals by means of two-step subtotal nephrectomy but. the S/P ratio was unchanged in comparison to the controls. Thus, renal failure may cause different changes in the salivary distribution of different types of drugs (Tomas et al., 2008). A few possibilities could be discussed on the mechanisms for enhanced salivary distribution of LOFLX in renal failure. In salivary gland, acinus cells form the barrier for drug translocation between blood and saliva. Renal insufficiency may induce destruction of the blood-saliva barrier leading to increase of drug distribution into saliva. In fact, detected amyloid-like

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Int. J. Pharmacol., 6 (6): 944-949, 2010 fibrils of a salivary gland in patients with renal insufficiency. Another possibility is related with the putative-specific transport in salivary glands. The activity of the possible efflux system for LOFLX may be inhibited by uremic toxins which increase in renal failure. resulting in accumulation of LOFLX in saliva. Alternatively, the possibility of the secondary change in the S/P ratio by elevated LOFLX levels in plasma and saliva induced by renal failure could be considered. Salivary distribution of LOFLX might be operated by a concentration-dependent system affected by elevation of the plasma and/or saliva concentration of the drug (Kinashi et al., 1989). The parallel bi-exponential decline of salivary LOFLX level with the plasma gave almost the same values for both and as those in the plasma level sample. suggesting that the salivary drug level may be substituted for the plasma level if consistent. S/P Talio is obtained over a broad concentration range. Since the LOFLX level in saliva was directly proportional to that in plasma, the plasma pharmacokinetics could be predicted almost completely. REFERENCES Albarellos, G.A., L.A. Ambros, M.F. Landoni, 2005. Pharmacokinetics of levofloxacin after single intravenous and repeat oral administration to cats. J. Vet. Pharmacol. Ther., 28: 363-369. Andersson, M.I. and A.P. MacGowan, 2003. Development of the quinolones. J. Antimicrob. Chemother, 51: 1-11. Czock, D., C. Ilusig-Linde, A. Langhoff, T. Schopke and C. Hafer et al., 2006. Pharmacokinetics of moxifloxacin and levofloxacin in intensive care unit patients who have acute renal failure and undergo extended daily dialysis. Clin. J. Am. Soc. Nephrol.. 1: 1263-1268. Edelstein, P.H., M.A.C. Edelstein, K.H. Lehr and J. Ren 1996. In vitro activity of levofloxacin against. clinical isolates of Legionella SPP ils pharmacokinetics in guinea pigs and use 111 experimental Legionella pneumophila pricumonia. J. Antimicrob. Chemother., 37: 117-126. Feinberg, I. and LG. Campbell, 1997. Coadministered pentobarbital anesthesia postpones but does not. block the motor and sleep EEG responses to MK-801. Life Sci., 60: 217-222. Garcia-Olmos, M., A. Parra, G. Garcia-Calvo, C. Ponte. M.J. Gimenez, L. Aguilar and F. Soriano, 2003. Efficacy and pharmacodynamics of gemifloxacin versus levofloxacin in guinea pig pneumococcal pneumonia induced by strains with decreased ciprofloxacin susceptibility. Int. J. Antimicrobial Agents, 21: 568-573. 949 Garrison, M.W., 2003. Comparative antimicrobial activity of levofloxacin and ciprofloxacin against Streptococcus pneumoniae. J. Antimicrob. Chemother, 52: 503-506. Giacomini, K.M., S.M. Roberts and G. Levy, 1981. Evaluation of methods for producing renal dysfunction in rats. J. Pharm. Sci., 70: 117-121. Johnson, R.D., M.B. Dorr, G.II. Talbot and G. Caille, 1988. Effect of maalox on the oral absorption of sparfloxacin. Clin Ther., 20: 11419-1158. Kinashi, M., S. Ohbayashi and M. Ohbayashi, 1989. Detection of amyloid-like fibrils using labial salivary gland biopsy in patients with nondialysis renal insufficiency. Am. J. Nephrol., 9: 435-437. Langlois, M.H., M. Montagul, J.P. Dubost, J. Grellet, and M.C. Saux, 2005. Protonation equilibrium and lipophilicity of moxifloxacin. J. Pharm. Biomed. Anal.. 37: 389-393. Novak, I., 1990. Salivary secretion Studies on intact. glands in vivo and in vitro. Methods Enzymol.. 192: 3-15. Perez-Ruiz, L., S. Ros-Lopez, A. Cardus, E. Fomandez and J.M. Valdivielso, 2006. A forgotten method to induce experimental chronic renal failure in the rat by ligation of the renal parenchyma. Nephron Exp. Nephrol., 103: 126-130. Santoro, M.I.R.M., N.M. Kassab, A.K. Singh and E.R.M. Kedor-Ilackmam, 2006. Quantitative determination of gatifloxacin, levofloxacin, lomefloxacin and pefloxacin fluoroquinolonic antibiotics in pharmaceutical preparations by high-performance liquid chromatography. J. Pharma. Biomed. Anal., 10: 179-181. 2002. Soriano, F and V. Rodriguez-Cerrato, Pharmacodynamic and kinetic basis for the selection of pneumococcal resistance in the upper respiratory tract. J. Antimicrob. Chemother. 50: 51-58. Thrivikraman, K.V., R.L. Huot and P.M. Plotsky, 2002. Jugular vein catheterization for repeated blood sampling in the unrestrained conscious ral. Brain Res. Protocols, 10: 84-94. Tomas, L., J.S. Marinho, J. Limeres, M.J. Santo, L. Arauja ankl P. Diz, 2008. Changes in salivary composition in patients with renal failure. Arch. Oral Biol.. 53: 528-532. and Tsaganosa, T., P. Koukib, P. Digenish, H. Giamarelloua. E.J. Giamarellos-Bourboulisa K. Kanellakopouloua, 2008. Pharmacokinetics of levofloxacin after single and multiple oral doses in patients undergoing intermittent haemodialysis. Int. J. Antimicrob. Agents, 32: 46-49. Zuidema, J., K.M. Ilold, D. Boer and R.A.A. Maes, 1996. Saliva as a specimen for therapeutic drug monitoring in pharmacies. Pharm. World Sci., 18: 193-191.