Quality characterization and hptlc fingerprinting of hingwadi choorna: a polyherbal formulation

Summary of article contents:

Ayurveda is known as the oldest healing science. Rising use of herbal drugs by the human is forcing the driving force to evaluate the health claim of these agents and to develop standards for a quality, purity, safety and efficacy of the drug. The present study was conducted with the aim to evaluate purity and strength of Hingwadi choorna . The prepared polyherbal formulation was subjected to determine the physical constants (pH determination, refractive index, specific gravity, total solids, reducing and nonreducing sugar) and HPTLC fingerprinting. The analysis revealed the physicochemical constant such as loss on drying 10.98%, total ash 15.32%, acid insoluble ash 1.4%, water soluble ash 12.8%, alcohol soluble extractive value 16.64% and water soluble extractive value 43.90%. HPTLC finger printing profile showed different band patterns at different wavelength under short UV, long UV and at 254 nm, 366 nm, and 620 nm after derivatisation with vanillin sulphuric acid spraying reagent. Unique Rfpatterns were recorded. Hingwadi choorna was authenticated according to pharmacopeial standards as its analysis was important to ensure the purity and strength of drug Keywords: Purity and Strength, Hingwadi choorna , HPTLC fingerprinting, physical constants INTRODUCTION Respiratory tract infections accounts about more than 50% of patients attending pediatric OPD in developing and even developed countries worldwide 1, 2. Use of Ayurvedic drugs inthe form of herbomineral formulation has been increased all over the world due to their huge therapeutic effect and less adverse effect as compared to other medicines. A polyherbal INTERNATIONAL AYURVEDIC MEDICAL JOURNAL International Ayurvedic Medical Journal, ( ISSN: 2320 5091) (October, 2017) 5(10) QUALITY CHARACTERIZATION AND HPTLC FINGERPRINTING OF HINGWADI CHOORNA : A POLYHERBAL FORMULATION Shraddha S R 1 , Chethan kumar V K 2 , Sharashchandra R 3 , Suchitra N Prabhu 4 1 PGScholar, 2 Associate Professor, 3 Assistant Professor,Department of Kaumarabhritya, Sri Dharmasthala Manjunatheshwara College of Ayurveda and Hospital, Udupi, Karnataka, India 4 Research officer, Pharmaceutical Chemistry and Pharmacognosy, SDM Center for Research inAyurveda and Allied Sciences, Udupi, KA, INDIA Email: drchethankumar@gmail.com ABSTRACT Ayurveda is known as the oldest healing science. Rising use of herbal drugs by the human is forcing the driving force to evaluate the health claim of these agents and to develop standards for a quality, purity, safety and efficacy of the drug. The present study was conducted with the aim to evaluate purity and strength of Hingwadi choorna . The prepared polyherbal formulation was subjected to determine the physical constants (pH determination, refractive index, specific gravity, total solids, reducing and nonreducing sugar) and HPTLC fingerprinting. The analysis revealed the physicochemical constant such as loss on drying 10.98%, total ash 15.32%, acid insoluble ash 1.4%, water soluble ash 12.8%, alcohol soluble extractive value 16.64% and water soluble extractive value 43.90%. HPTLC finger printing profile showed different band patterns at different wavelength under short UV, long UV and at 254 nm, 366 nm, and 620 nm after derivatisation with vanillin sulphuric acid spraying reagent. Unique Rfpatterns were recorded. Hingwadi choorna was authenticated according to pharmacopeial standards as its analysis was important to ensure the purity and strength of drug Keywords: Purity and Strength, Hingwadi choorna , HPTLC fingerprinting, physical constants INTRODUCTION Respiratory tract infections accounts about more than 50% of patients attending pediatric OPD in developing and even developed countries worldwide 1, 2. Use of Ayurvedic drugs inthe form of herbomineral formulation has been increased all over the world due to their huge therapeutic effect and less adverse effect as compared to other medicines. A polyherbal INTERNATIONAL AYURVEDIC MEDICAL JOURNAL International Ayurvedic Medical Journal, ( ISSN: 2320 5091) (October, 2017) 5(10) QUALITY CHARACTERIZATION AND HPTLC FINGERPRINTING OF HINGWADI CHOORNA : A POLYHERBAL FORMULATION Shraddha S R 1 , Chethan kumar V K 2 , Sharashchandra R 3 , Suchitra N Prabhu 4 1 PGScholar, 2 Associate Professor, 3 Assistant Professor,Department of Kaumarabhritya, Sri Dharmasthala Manjunatheshwara College of Ayurveda and Hospital, Udupi, Karnataka, India 4 Research officer, Pharmaceutical Chemistry and Pharmacognosy, SDM Center for Research inAyurveda and Allied Sciences, Udupi, KA, INDIA Email: drchethankumar@gmail.com ABSTRACT Ayurveda is known as the oldest healing science. Rising use of herbal drugs by the human is forcing the driving force to evaluate the health claim of these agents and to develop standards for a quality, purity, safety and efficacy of the drug. The present study was conducted with the aim to evaluate purity and strength of Hingwadi choorna . The prepared polyherbal formulation was subjected to determine the physical constants (pH determination, refractive index, specific gravity, total solids, reducing and nonreducing sugar) and HPTLC fingerprinting. The analysis revealed the physicochemical constant such as loss on drying 10.98%, total ash 15.32%, acid insoluble ash 1.4%, water soluble ash 12.8%, alcohol soluble extractive value 16.64% and water soluble extractive value 43.90%. HPTLC finger printing profile showed different band patterns at different wavelength under short UV, long UV and at 254 nm, 366 nm, and 620 nm after derivatisation with vanillin sulphuric acid spraying reagent. Unique Rfpatterns were recorded. Hingwadi choorna was authenticated according to pharmacopeial standards as its analysis was important to ensure the purity and strength of drug Keywords: Purity and Strength, Hingwadi choorna , HPTLC fingerprinting, physical constants INTRODUCTION Respiratory tract infections accounts about more than