Standardization of an ayurvedic drug - madhumeha kusumakar rasa by hptlc

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Madhumeha Kusumakar Rasa (MKR) is an Ayurvedic medicine having ingredients of Vasantkusumakar Rasa , Shuddha Shilajeet , Jasad Bhasma , Extract of Mamajjaka ( Enicostemma Littorale ), Haridra ( Curcuma longa ), Amalaki ( Emblica officinalis ) and Guduchi ( Tinospora cordifolia ) indicated for complications of madhumeha (Diabetes). The purpose of this work was to develop and validate HPTLC method for quantification of marker compound Swertiamarin which is expressed in formulation through one of its major ingredient ( Pradhan dravya ) Mamajjaka ( Enicostemma Littorale ). The formulation was subjected to methanol extractions and extracted samples were applied on TLC plate precoated with Silica Gel 60 GF 254 The detection and quantification was performed at a wavelength of 240 nm. The methodvalidation was carried out as per ICH guidelines. Calibration curve plotted was found to be linear in the range of 200– 900 ng. The linear regression equation was found to be Y = 3.50 X + 670.5, while correlation coefficient (r 2) was 0.9996 with high reproducibility and accuracy. LOD and LOQ were found tobe 67.8 and 205.6 ng respectively. Madhumeha Kusumakar Rasa samples MKR-1, MKR-2 and MKR-3 were found to contain Swertiamarin 15.08 mg/tab, 14.95 mg/tab and 14.30 mg/tab respectively. This method was thus found to be linear, precise and accurate for quantitative determination of Swertiamarin in MKR Keywords: Madhumeha Kusumakar Rasa, Mamajjaka, Swertiamarin , HPTLC INTRODUCTION Ayurved is one of the widely practicedand recognized systems of medicine in India. This traditional system of medicine recommends usage of herbs and minerals in formulations for holistic health care and cure to various ailments and health disorders Madhumeha Kusumakar Rasa has beenindicated in various complication of Madhumeha like Madhumehajanya netravikar , non healing diabetic wound, polyuria, naktamootrata etc. This formulation consist of ingredients like Suvarnayukta Vasant Kusumakar Rasa , Mamajjaka Ghana , Amalaki , Haridra which are reported for reducing blood and urine sugar effectively due to their kapha and meda pachak action[1] Enicostemma Littorale Blume is taxonomically classified in family Gentianace un- Research Article International Ayurvedic Medical Journal ISSN:2320 5091 STANDARDIZATION OF AN AYURVEDIC DRUG - MADHUMEHA KUSUMAKAR RASA BY HPTLC K S Thakur 1 , P P Pimpalkar 2 , M D Gawhankar 3 , R V Gudi 4 1,4 Shree Dhootapapeshwar Limited, 2,3 Shree Dhootapapeshwar Ayurvedic Research Foundation,Veer Savarkar Chowk, Panvel, Navi Mumbai, Maharashtra, India ABSTRACT Madhumeha Kusumakar Rasa (MKR) is an Ayurvedic medicine having ingredients of Vasantkusumakar Rasa , Shuddha Shilajeet , Jasad Bhasma , Extract of Mamajjaka ( Enicostemma Littorale ), Haridra ( Curcuma longa ), Amalaki ( Emblica officinalis ) and Guduchi ( Tinospora cordifolia ) indicated for complications of madhumeha (Diabetes). The purpose of this work was to develop and validate HPTLC method for quantification of marker compound Swertiamarin which is expressed in formulation through one of its major ingredient ( Pradhan dravya ) Mamajjaka ( Enicostemma Littorale ). The formulation was subjected to methanol extractions and extracted samples were applied on TLC plate precoated with Silica Gel 60 GF 254 The detection and quantification was performed at a wavelength of 240 nm. The methodvalidation was carried out as per ICH guidelines. Calibration curve plotted was found to be linear in the range of 200– 900 ng. The linear regression equation was found to be Y = 3.50 X + 670.5, while correlation coefficient (r 2) was 0.9996 with high reproducibility and accuracy. LOD and LOQ were found tobe 67.8 and 205.6 ng respectively. Madhumeha Kusumakar Rasa samples MKR-1, MKR-2 and MKR-3 were found to contain Swertiamarin 15.08 mg/tab, 14.95 mg/tab and 14.30 mg/tab respectively. This method was thus found to be linear, precise and accurate for quantitative determination of Swertiamarin in MKR Keywords: Madhumeha Kusumakar Rasa, Mamajjaka, Swertiamarin , HPTLC INTRODUCTION Ayurved is one of the widely practicedand recognized systems of medicine in India. This traditional system of medicine recommends usage of herbs and minerals in formulations for holistic health care and cure to various ailments and health disorders Madhumeha Kusumakar Rasa has beenindicated in various complication of Madhumeha like Madhumehajanya netravikar , non healing diabetic wound, polyuria, naktamootrata etc. This formulation consist of ingredients like Suvarnayukta Vasant Kusumakar Rasa , Mamajjaka Ghana , Amalaki , Haridra which are reported for reducing blood and urine sugar effectively due to their kapha and meda pachak action[1] Enicostemma Littorale Blume is taxonomically classified in family Gentianace un- Research Article International Ayurvedic Medical Journal ISSN:2320 5091 STANDARDIZATION OF AN AYURVEDIC DRUG - MADHUMEHA KUSUMAKAR RASA BY HPTLC K S Thakur 1 , P P Pimpalkar 2 , M D Gawhankar 3 , R V Gudi 4 1,4 Shree Dhootapapeshwar Limited, 2,3 Shree Dhootapapeshwar Ayurvedic Research Foundation,Veer Savarkar Chowk, Panvel, Navi Mumbai, Maharashtra, India ABSTRACT Madhumeha Kusumakar Rasa (MKR) is an Ayurvedic medicine having ingredients of Vasantkusumakar Rasa , Shuddha Shilajeet , Jasad Bhasma , Extract of Mamajjaka ( Enicostemma Littorale ), Haridra ( Curcuma longa ), Amalaki ( Emblica officinalis ) and Guduchi ( Tinospora cordifolia ) indicated for complications of madhumeha (Diabetes). The purpose of this work was to develop and validate HPTLC method for quantification of marker compound Swertiamarin which is expressed in formulation through one of its major ingredient ( Pradhan dravya ) Mamajjaka ( Enicostemma Littorale ). The formulation was subjected to methanol extractions and extracted samples were applied on TLC plate precoated with Silica Gel 60 GF 254 The detection and quantification was performed at a wavelength of 240 nm. The methodvalidation was carried out as per ICH guidelines. Calibration curve plotted was found to be linear in the range of 200– 900 ng. The linear regression equation was found to be Y = 3.50 X + 670.5, while correlation coefficient (r 2) was 0.9996 with high reproducibility and accuracy. LOD and LOQ were found tobe 67.8 and 205.6 ng respectively. Madhumeha Kusumakar Rasa samples MKR-1, MKR-2 and MKR-3 were found to contain Swertiamarin 15.08 mg/tab, 14.95 mg/tab and 14.30 mg/tab respectively. This method was thus found to be linear, precise and accurate for quantitative determination of Swertiamarin in MKR Keywords: Madhumeha Kusumakar Rasa, Mamajjaka, Swertiamarin , HPTLC INTRODUCTION Ayurved is one of the widely practicedand recognized systems of medicine in India