Phytochemical analysis of Dichrostachys cinerea leaves via chromatography.
Journal name: Ancient Science of Life
Original article title: Phytochemical analysis of ethanolic extract of Dichrostachys Cinerea W and Arn leaves by a thin layer chromatography, high performance thin layer chromatography and column chromatography
The ANSCI is a peer-reviewed, open-access journal focused on Ayurveda and traditional medicines. It publishes original research, reviews, and literary studies linking traditional knowledge with modern science, covering disciplines like botany, ethnomedicine, pharmacology, and clinical research.
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Original source:
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M. Vijayalakshmi, K. Periyanayagam, K. Kavitha, K. Akilandeshwari
Ancient Science of Life:
(A quarterly multi-disciplinary scientific research journal in Ayurveda)
Full text available for: Phytochemical analysis of ethanolic extract of Dichrostachys Cinerea W and Arn leaves by a thin layer chromatography, high performance thin layer chromatography and column chromatography
Year: 2013
Copyright (license): CC BY-NC-SA
Summary of article contents:
Introduction
The study investigates the phytochemical properties of the leaves of Dichrostachys cinerea, a perennial shrub native to regions in North West India and Central Australia. The leaves possess a variety of medicinal uses, including laxative, diuretic, pain-relieving, and treatments for ailments such as gonorrhea, boils, and edema. The research employs various analytical techniques, including thin layer chromatography (TLC), high performance thin layer chromatography (HPTLC), and column chromatography to extract and analyze the phytochemical constituents present in the ethanolic extract of the leaves.
Isolation of β-amyrin Glycoside
A significant finding of the study is the isolation of β-amyrin glycoside from the ethanolic extract of D. cinerea leaves. The research outlined the procedure followed for extracting the compounds, which included defatting the leaf powder and extracting it with ethanol, followed by chromatographic techniques. The β-amyrin glycoside was confirmed using various spectral studies including Infrared (IR) spectroscopy, Nuclear Magnetic Resonance (NMR), and Mass spectrometry. This unique glycoside is reported for the first time from the leaves of D. cinerea, emphasizing the plant's potential as a source of medicinal compounds that could warrant further exploration and validation for therapeutic applications.
Conclusion
This study highlights the importance of herbal medicines as a rich source of bioactive compounds, showcasing the methodology for identifying and characterizing phytochemicals present in plant extracts. The findings contribute to the pharmacognostic knowledge of D. cinerea, specifically through the identification of β-amyrin glycoside, paving the way for further research into its potential health benefits. Moreover, the methodologies employed provide a standard framework for the analysis and identification of other herbal drugs, underscoring the necessity of phytochemical investigations for the purpose of proper identification and standardization in herbal medicine.
FAQ section (important questions/answers):
What are the therapeutic uses of Dichrostachys cinerea leaves?
Dichrostachys cinerea leaves are used for various medicinal purposes, including treatment of laxatives, diuretics, pain relief, gonorrhea, boils, edema, gout, and skin infections.
Which phytochemical tests were conducted on the extract?
The phytochemical analysis involved standard chemical tests, thin layer chromatography (TLC), high-performance thin layer chromatography (HPTLC), and column chromatography to identify and isolate various compounds from the ethanolic extract.
What compounds were found in the ethanolic extract of leaves?
Preliminary phytochemical screening revealed the presence of carbohydrates, proteins, glycosides, saponins, tannins, amino acids, and terpenoids in the ethanolic extract of Dichrostachys cinerea leaves.
What was isolated from the dichrostachys cinerea leaves?
The compound isolated from the leaves was identified as β-amyrin glycoside, confirmed through multiple spectroscopic methods, marking its first report in Dichrostachys cinerea.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Phytochemical analysis of Dichrostachys cinerea leaves via chromatography.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Phytochemical:
Phytochemical refers to the chemical compounds produced by plants, which are studied for their potential health benefits. These compounds often exhibit pharmacological properties, which can contribute to various therapeutic effects. Phytochemical analysis helps identify beneficial substances in medicinal plants, aiding in the development of herbal medicines and dietary supplements.
2) Anna:
Anna likely refers to Anna University, an educational institution in India mentioned in the article. It is known for its research in fields like Pharmaceutical Technology. The university's involvement signifies the academic and research contributions to the study of medicinal plants, emphasizing the connection between education and pharmaceutical research.
3) Drug:
In pharmacology, a drug is any substance used to diagnose, cure, treat, or prevent disease. The article's context involves evaluating the potential medicinal properties of Dichrostachys cinerea leaves. Understanding drug efficacy is important for developing treatments and recognizing natural sources of therapeutic agents from plants.
4) Discussion:
Discussion represents a critical section of scientific research where results are analyzed and interpreted. It provides insights into findings, contextualizes them with existing knowledge, and presents implications for future research. In the provided text, the discussion to evaluates the significance of the phytochemical screening and the isolated compound in potential therapies.
5) Flavonoid:
Flavonoids are a diverse group of plant metabolites known for their antioxidant properties. They play a role in human health by protecting against diseases and offering anti-inflammatory effects. The absence of flavonoids in the studied phytochemical analysis highlights specific compound profiles in the leaves of Dichrostachys cinerea.
6) Swelling:
Swelling is an increase in volume or size of a body part, often associated with inflammation or injury. The article mentions the use of Dichrostachys cinerea for conditions such as edema and swelling, indicating the plant’s potential therapeutic role in reducing inflammation and promoting healing processes.
7) Activity:
Activity in the context of pharmacological studies signifies the efficacy and biological responses elicited by compounds. The study notes the antilice activity of the ethanolic extract of Dichrostachys cinerea leaves, emphasizing the importance of evaluating natural extracts in their capability to affect physiological processes and treat health issues.
8) Coconut (Cocoanut):
Coconut, mentioned in the context of the carrier for the ethanolic extract in the study, is a natural oil known for its various health benefits. It serves as a medium in herbal formulations and enhances the bioavailability of active compounds, demonstrating the relevance of choosing appropriate carriers for herbal extracts.
9) Science (Scientific):
Science refers to the systematic study of the natural world through observation and experimentation. The research discussed in the article embodies scientific principles, contributing to the body of knowledge surrounding medicinal plants, phytochemistry, and pharmacognosy, providing a foundation for future researches in health and medicine.
10) Edema (Oedema):
Edema is a similar term to oedema, characterized by excess fluid accumulation in tissues, leading to swelling. The article highlights the therapeutic applications of Dichrostachys cinerea leaves in treating edema, reinforcing the necessity of studying plant extracts' anti-inflammatory and diuretic properties as natural treatment alternatives.
11) Glass:
Glass indicates the material used for laboratory equipment, essential in experimental contexts, such as chromatography or spectrometry. It ensures the proper containment and analysis of solvents and extracts in the studied methodology, facilitating the evaluation of the phytochemical properties and effective isolation of compounds in research.
12) Sugar:
In the biochemistry context, sugar refers to carbohydrates which can be part of plant extracts. The study identifies the presence of sugars in the ethanolic extract of Dichrostachys cinerea, which can have implications for energy metabolism and potential health benefits of the extract, enhancing its nutritional value.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Phytochemical analysis of Dichrostachys cinerea leaves via chromatography.’. Further sources in the context of Science might help you critically compare this page with similair documents:
Dichrostachys cinerea, Medicinal plant, High performance thin layer chromatography, Phytochemical screening, Thin Layer Chromatography, Phytochemical analysis, Ethanolic extract, Column chromatography, Preliminary phytochemical tests, Department of Pharmacognosy, Spectral studies, Department of Pharmaceutical Technology.