In-vitro callus and root culture of Boerhaavia diffusa Linn.
Journal name: Ancient Science of Life
Original article title: In-vitro culture studies for callus and root generation of boerhaavia diffusa linn
The ANSCI is a peer-reviewed, open-access journal focused on Ayurveda and traditional medicines. It publishes original research, reviews, and literary studies linking traditional knowledge with modern science, covering disciplines like botany, ethnomedicine, pharmacology, and clinical research.
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Original source:
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Vandana Jain, D. Singh, Swarnalata Saraf, S. Saraf
Ancient Science of Life:
(A quarterly multi-disciplinary scientific research journal in Ayurveda)
Full text available for: In-vitro culture studies for callus and root generation of boerhaavia diffusa linn
Year: 2003
Copyright (license): CC BY-NC-SA
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Summary of article contents:
Introduction
The study investigates the in vitro culture of Boerhaavia diffusa Linn, commonly known as Punarnava, focusing on callus and root generation. This plant belongs to the family Nyctaginaceae and is primarily found in high-altitude regions, particularly in the hot Himalayan valleys. Recognized for its active constituents, including punarnavoside and flavonoids, B. diffusa exhibits anti-fibrinolytic, anti-inflammatory, and hepatoprotective properties. Due to its significance in traditional Indian medicine, the research aimed to optimize culture conditions in order to enhance the biosynthesis pathways of its bioactive compounds.
Callus Culture Optimization
A significant aspect of the study was the optimization of culture media for callus formation. The research found that leaf explants produced superior callus compared to stem explants when cultured on Murashige and Skoog (MS) medium supplemented with various plant growth regulators. Specifically, a concentration of 20µM 2,4-D resulted in the highest response for callus generation, yielding a remarkable 979.5% and an induction period of just 8 days. The callus obtained was described as friable and opaque, showcasing favorable characteristics for potential applications. Although picrolam also promoted callus formation, the results were inferior in terms of average weight when compared to 2,4-D.
Conclusion
In conclusion, the study successfully establishes optimized conditions for in vitro culture of B. diffusa, revealing that 20µM 2,4-D is ideal for callus generation and that 5µM IBA is optimal for root development. Leaf explants were determined to be more effective than stem explants for both rooting and callus formation. The findings highlight the potential for further research to enhance the yields of active principles through additional modifications in the culture medium or by introducing suitable precursors. This work contributes to the broader understanding of plant tissue culture techniques applicable to medicinal plants.
FAQ section (important questions/answers):
What plant was studied for in vitro culture in this research?
The study focused on Boerhaavia diffusa Linn, commonly known as Punarnava, which is valued in traditional Indian medicine for its various medicinal properties.
What type of explants were used for the culture studies?
Leaf and stem explants excised from young plants of B. diffusa were used. Leaf explants showed a better response for rooting and callus formation compared to stem explants.
Which hormone concentration was optimal for root generation?
An IBA concentration of 5µM was identified as optimal for root generation, yielding a maximum regeneration rate of 69.7% with an induction period of 7 days.
What results were observed for callus formation in this study?
The results showed that 20µM of 2,4-D produced the highest callus formation response, with an average of 979.5% and an induction period of 8 days.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “In-vitro callus and root culture of Boerhaavia diffusa Linn.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Agriculture:
Agriculture relates to the cultivation of plants, such as Boerhaavia diffusa, which is studied for its medicinal properties. Understanding agricultural practices is crucial for obtaining healthy plants and ensuring the proper extraction and understanding of plant constituents. This study emphasizes the importance of plant cultivation methods in enhancing bioactive compound yield.
2) Discussion:
Discussion in the context of this study refers to the analysis and interpretation of the results obtained from in vitro culture experiments. It highlights the significance of findings regarding callus formation and rooting in Boerhaavia diffusa, considering various plant growth regulators. The discussion helps in understanding the underlying mechanisms and guiding future research.
3) Punarnava (Punarnavā, Punarṇava, Punar-nava):
Punarnava, the common name for Boerhaavia diffusa, is significant for its medicinal applications in traditional Indian medicine. It possesses various therapeutic properties, such as anti-inflammatory, diuretic, and hepatoprotective effects. This study specifically investigates its propagation and callus culture to improve the yield of active principles derived from this valuable plant.
4) Science (Scientific):
Science in this context pertains to the systematic study and application of biological processes, especially plant biology and biotechnology. The research employs scientific methods for in vitro culture, focusing on regeneration and bioactive compound synthesis in Boerhaavia diffusa. It exemplifies how scientific exploration can enhance our understanding of medicinal plants.
5) Shukla (Śukla, Śuklā, Suklā, Sukla):
Shukla refers to Pt. Ravishankar Shukla University, an academic institution involved in the study. The mention underscores the collaboration between various institutions to conduct rigorous scientific research on Boerhaavia diffusa. It serves to highlight the significance of institutional support in advancing studies related to medicinal plants and their cultivation.
6) Water:
Water is a fundamental component in plant tissue culture, essential for hydration and nutrient transport. The study emphasizes the washing steps to sterilize explants, ensuring that microbial contamination does not compromise the experiments. Proper water quality and usage are critical for successful in vitro propagation and growth of Boerhaavia diffusa.
7) Mura:
Mura likely references 'Murashige and Skoog' (MS) medium, a widely used plant tissue culture medium. It provides necessary nutrients, vitamins, and growth regulators for plant cell growth. The study utilizes MS medium as a culture base for Boerhaavia diffusa, demonstrating its importance in plant regeneration and callus formation.
8) Vari (Vāri):
Vari refers to varying concentrations of plant growth regulators tested in the study. It emphasizes the experimental approach taken to optimize conditions for callus and root generation in Boerhaavia diffusa. Understanding the effects of different concentrations is crucial for identifying the most effective conditions for enhancing plant tissue culture outcomes.
Other Science Concepts:
Discover the significance of concepts within the article: ‘In-vitro callus and root culture of Boerhaavia diffusa Linn.’. Further sources in the context of Science might help you critically compare this page with similair documents:
High altitude, Diuretic properties, Incubation period, Hepatoprotective properties, Active constituent, Callus formation, Ancient Science of life, Surface sterilization, Murashige and Skoog medium, Fresh weight, 2,4-D, Dry weight, Stem explants, Leaf explant.