International Journal of Pharmacology

2005 | 8,224,669 words

The International Journal of Pharmacology (IJP) is a globally peer-reviewed open access journal covering the full spectrum of drug and medicine interactions with biological systems, including chemical, physiological, and behavioral effects across areas such as cardiovascular, neuro-, immuno-, and cellular pharmacology. It features research on drug ...

Enhanced Bioavailability of Epigallocatechin Gallate (EGCG) after...

Author(s):

Sheng-Chang Lin
School of Nutrition, Chung Shan Medical University, 110, Sec. 1, Jianguo North Road, Taichung City, Taiwan, Republic of China
Hui-Fang Chiu
Department of Chinese Medicine, Taichung Hospital, Ministry of Health and Well-being, Taichung, Taiwan, Republic of China
Yun-Chen Hsieh
School of Nutrition, Chung Shan Medical University, 110, Sec. 1, Jianguo North Road, Taichung City, Taiwan, Republic of China
Kamesh Venkatakrishnan
School of Nutrition, Chung Shan Medical University, 110, Sec. 1, Jianguo North Road, Taichung City, Taiwan, Republic of China
Oksana Golovinskaia
Department of Food Biotechnology, ITMO University, 9, Lomonosova street, 191002, Saint Peterburg, Russia
Chin-Kun Wang
School of Nutrition, Chung Shan Medical University, 110, Sec. 1, Jianguo North Road, Taichung City, Taiwan, Republic of China


Year: 2022 | Doi: 10.3923/ijp.2022.623.632

Copyright (license): Creative Commons Attribution 4.0 International (CC BY 4.0) license.


[Full title: Enhanced Bioavailability of Epigallocatechin Gallate (EGCG) after Esterification and Complexation with Fish Oil]

INTRODUCTION

Green tea is the second most consumed beverage in the world due to its numerous beneficial properties. Many researchers have indicated that green tea is rich in catechins especially epigallocatechin gallate (EGCG), which is responsible for major biological functions including antioxidant, anti-inflammatory, anti-diabetic, anti-obesity, anti-hypertensive and anti-microbial properties1,2. However, the biological function of green tea is much limited due to the low bioavailability of EGCG (active phytocomponent)3,4. The major reason for the low bioavailability of EGCG including high sensitivity towards pH, light, temperature and ionic strength with high polarity nature (hydrophilicity) and its high reactivity property (especially with digestive enzymes) which makes EGCG more unstable and easily oxidizable5,6. The Pharmacokinetics studies of EGCG in animal models revealed that only less than 5% of tea catechins (EGCG) could reach the systemic circulation. Likewise, in human also less than 2% of EGCG was detected in blood after consumption of green tea (rich in EGCG) with 3 to 4.5 hrs of half-life and thus both the animal and human studies indicate that EGCG shows lower bioavailability and bioaccessibility3,7,8. Therefore, many researchers started to focus on improving the bioavailability of EGCG by complexation or esterification of EGCG with various components like piperine, curcumin, ascorbic acid, proteins (albumin, lactoglobulin) by changing the physical and chemical properties of EGCG9-11. Moreover, recently our team also conducted a study by combining EGCG with royal jelly proteins (Major royal jelly protein-MRJP) and the EGCG-MRJP complex showed better bioavailability of EGCG with increased EGCG cellular uptake in the cell model3.

Fish oil is primarily composed of omega-3-fatty acids such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), which are considered as major ingredients responsible for various health benefits12. Ample amounts of studies indicated that EPA and DHA show an array of biological functions including antioxidant, anti-inflammatory, anticancer, antidiabetic and antihyperlipidemic properties2,13. However, the stability of DHA and EPA is lowered due to low water solubility (lipophilicity), increased light and pH sensitivity and high oxidation susceptibility (autoxidation-lipid peroxidation) which results in lower bioavailability in human14,15 as similar to EGCG. Hence, the author hypothesizes that a combination of both EGCG (water-soluble) and EPA/DHA (lipid-soluble) would be a better idea to make both nutraceuticals for improved bioavailability (both protect each other). For the same reason, few scholars esterified EGCG with various fatty acids (fatty acid esters-acyl donor) and found

better bioavailability16,17. However, the above-mentioned studies lack to check the other beneficial efficiency of those esterified compounds as well as not compared the effect of esterified EGCG with EGCG complex. Hence, this novel study was designed to evaluate the beneficial efficacy of esterified EGCG with fish oil (DHA/EPA) by assessing the esterification efficiency and permeability ability (bioavailability of EGCG) as well as its various biological function by exploring the oxidative capacity and anti-glycation activity on C2BBe1 cells by comparing with EGCG and EGCG-Fish oil complex.

MATERIALS AND METHODS

Samples/chemicals: EGCG (98% HPLC grade) was purchased from Hunan Sunfull Biotech Co., Ltd. (Hunan, China). Fish oil (as a capsule) rich in DHA/EPA was provided by Herbalife Nutrition Corp (CA, USA). All the experiments were carried out at Chung Shan Medical University at School of Nutrition, Taichung, Taiwan from 2018-2019.

Separation/isolation of DHA and EPA from fish oil: In short, the Free Fatty Acids (FFA) were separated from the fish oil capsule by saponification procedure and followed by urea complexation procedure to purify only DHA and EPA from other FFAs as indicated by Zhong and Shahidi16. Then the presence of DHA and EPA in FFA was confirmed by TLC (supplement data).

Esterification/complexation of EGCG with FFA (DHA/EPA): Esterification of EGCG with isolated FFA rich in DHA/EPA based on the method described by Sekhon-Loodu and Rupasinghe18 using Lipase B (Novozym 435) to trigger acylation. The efficiency of esterification and complexation is confirmed by checking the levels of EPA, DHA and EGCG using various techniques like NMR spectroscopy and GC-MS as mentioned by Zhong and Shahidi16.

Antioxidant indexes
TEAC, DPPH scavenging activities: Trolox equivalent antioxidant capacity (TEAC) or total antioxidant capacity of different samples (EGCG, EGCG-esters and EGCG-Fish oil) was determined using the Arnao method19 and was expressed as μg Trolox eq mg–1. The DPPH scavenging activity was examined by the method of Shimada and his colleagues20 using the below formula:

Image for - Enhanced Bioavailability of Epigallocatechin Gallate (EGCG) after Esterification and Complexation with Fish Oil

DPPH scavenging activity is expressed in percentage and also different standards are used for comparison.

Cell line: For the current cell study, the human colon epithelial adenocarcinoma C2BBe1 cells (clonal of Caco2 cells: CRL-2102) were bought from ATCC (Tainan, Taiwan) and cultured with Dulbecco’s modified Eagle medium (DMEM) (Sigma-Aldrich, St. Louis, MO, USA) and supplemented with 1% L glutamine, 1% penicillin-streptomycin, 10% Fetal Bovine Serum (FBS), 1.5 g L–1 sodium bicarbonate (Sigma-Aldrich, St. Louis, MO, USA). The C2BBe1 cells were maintained in a humidified atmosphere (5% CO2) at 37°C using a CO2 incubator.

Cell viability (Cytotoxicity) and morphology analysis: The C2BBel cells viability of various experimental samples (EGCG, EGCG-Esters and EGCG-Fish oil) at different duration (24 and 48 hrs) were checked using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay as mentioned by Han and his coworkers3. Then, the morphological changes in C2BBel cells were also checked after the addition of various experimental samples (EGCG, EGCG-Esters and EGCG-Fish oil) under a compound microscope (10X) by comparing with control cells for any morphological changes or abnormalities.

EGCG cell uptake or permeability ability: To assess the EGCG cellular uptake (permeability coefficient), the C2BBel cells were grown and treated with various experimental samples (100 μg mL–1 of EGCG, EGCG-Esters and EGCG-Fish oil) for 24 and 48 hrs under 37°C. Then the cells were dissolved in PBS solution (Sigma-Aldrich, St. Louis, MO, USA) and lysed with sonicator and centrifuged (5000 rpm for 10 min) and the resultant supernatant was filtered and EGCG levels were quantified using HPLC and the permeability coefficient were calculated using the formula as indicated by our previous study3.

Antiglycation activity: Antiglycation activity (AGEs inhibition activity) of various experimental samples (100 μg mL–1 of EGCG, EGCG-Esters and EGCG-Fish oil) in C2BBel cells were calculated using BSA-MGO and BSA-Fructose model (protein glycation model) by the methods mentioned by Wang and others21 and Shen and others22. Aminoguanidine (AGEs trapper) was used as a positive control. The AGEs inhibition was calculated using the below formula and expressed in percentage.

Image for - Enhanced Bioavailability of Epigallocatechin Gallate (EGCG) after Esterification and Complexation with Fish Oil

Statistical data: All the cell line studies are conducted in triplicate (n = 3) and all the data are exemplified as the Mean±Standard Deviation (SD). All the data were analyzed using one-way ANOVA followed by Tukey's multi-range test using SPSS software (IBM Corp., USA). A p-value less than 0.05 is considered statistically significant.

RESULTS

Confirmation of esterification (EGCG-Ester): To confirm the esterification (efficiency) of EGCG with free fatty acids like DHA and EPA, we used 13CNMR and GC-MS techniques. The NMR spectrum including EGCG ester (EGCG-DHA/EPA) was shown in Fig. 1. The LC-MS spectrum of all the fatty acids in Fish oil was shown in Fig. 2a. Whereas Fig. 2b represents the LS-MS spectrum including EGCG (alone), standards of DHA and EPA, EGCG-Ester and EGCG-Fish oil complex. Both, NMR and LC-MS spectrum shows the esterification of EGCG with DHA and EPA. Moreover, TLC was used to confirm the presence of DHA and EPA in free fatty acid (from fish oil) before esterification (Fig. S1).

Antioxidant status: The antioxidant activity of different samples (EGCG, EGCG-Esters and EGCG-Fish oil) on C2BBel cells were determined by TEAC, DPPH scavenging activity (Table 1). EGCG-ester (EGCG-DHA/EPA) group showed better TEAC (17.97±1.14) and DPPH (96.7%) scavenging activity as compared to EGCG or EGCG-fish oil complex group.

Table 1: Trolox equivalent antioxidant capacity (TEAC) or total antioxidant capacity, DPPH scavenging activity of different samples (EGCG, EGCG-Esters and EGCG-fish oil) on C2BBel cells Samples TEAC (μg trolox eq mg–1) DPPH scavenging activity (inhibition %) EGCG 15.65±1.15b 95.5±10.15b EGCG-ester 17.97±1.14a 96.7±09.15a EGCG-fish oil complex 13.49±1.10c 94.8±10.15c Data are expressed as the Mean±Standard deviation (n = 3). Data within the same column sharing different superscript letters (a, b, c) were statistical differences (p<0.05)

Image for - Enhanced Bioavailability of Epigallocatechin Gallate (EGCG) after Esterification and Complexation with Fish Oil Fig. 1: 13CNMR spectrum, showing EGCG-Ester(rich in DHA and EPA)

In vitro studies (Cell model)
Cell viability (Cytotoxicity) and morphology analysis: The C2BBe1 cells viability efficacy (cytotoxicity) of various experimental samples like EGCG, EGCG-Ester and EGCG-Fish oil complex were checked by MTT assay and was shown in Fig. 3. The C2BBe1 cells treated with 100, 200 and 500 μg mL–1 of EGCG, EGCG-Ester and EGCG-Fish oil complex did not show any significant difference between each group at 24 and 48 hrs. Thus, indicating no cytotoxicity activity in any of the experimental drugs (EGCG, EGCG-Ester and EGCG-Fish oil complex). Moreover, C2BBe1 cells treated with different experimental samples (EGCG, EGCG-Ester and EGCG-Fish oil complex), did not show any morphological changes under the microscope (Fig. S2).

EGCG cell uptake or permeability ability: The cellular uptake (permeability efficiency) of EGCG of different samples (EGCG, EGCG-Ester and EGCG-Fish oil complex) on C2BBe1 cells at a different time interval (2, 24 and 48 hrs) was shown in Fig. 4. The EGCG cell uptake (apical transmembrane permeability) was calculated based on the permeability coefficient. At 2, 24 and 48 hrs, the EGCG-ester group (100 μg mL–1 showed significantly higher levels (p<0.05) of EGCG uptake than EGCG alone or EGCG-Fish oil group. Based on the above results it's clear that esterified EGCG (EGCG-DHA/EPA) showed the best EGCG uptake ability.

Antiglycation activity: The antiglycation activity (AGEs inhibition activity) of various experimental samples (100 μg mL–1 of EGCG, EGCG-Esters and EGCG-Fish oil) on C2BBel cells were assessed by BSA-MGO (Fig. 5a) and BSA-Fructose (Fig. 5b) models.

Image for - Enhanced Bioavailability of Epigallocatechin Gallate (EGCG) after Esterification and Complexation with Fish Oil

Fig. 2(a-b): LC-MS spectrum of all the fatty acids in Fish oil (a) and chromatograms of EGCG, DHA, EPA, EGCG-EPA Ester, EGCG-DHA Ester, and EGCG-Fish oil complex at 210 nm (b)

The C2BBel cells treated with MGO and fructose trigger BSA protein glycation but the addition of samples like EGCG, EGCG-Esters and EGCG-Fish oil lower the AGEs production by trapping MGO and fructose. However, EGCG display potent AGEs inhibition activity in both MGO and fructose models than EGCG-Esters and EGCG-Fish oil complex groups. Since EGCG alone group has any free hydroxyl group which might effectively trap MGO and form adduct and thus lower AGES production. Nevertheless, the esterified EGCG and EGCG-fish oil complex lacks many free hydroxyl groups and hence showed lower AGEs inhibitor activity than the EGCG group.

Image for - Enhanced Bioavailability of Epigallocatechin Gallate (EGCG) after Esterification and Complexation with Fish Oil

Fig. 3(a-c): Viability efficacy of various experimental samples (a) EGCG, (b) EGCG-Ester and (c) EGCG-Fish oil complex (3C) on C2BBe1 cells

Data are expressed as the Mean±Standard deviation (n = 3)

Image for - Enhanced Bioavailability of Epigallocatechin Gallate (EGCG) after Esterification and Complexation with Fish Oil

Fig. 4: Cellular uptake (permeability efficiency) of EGCG on C2BBe1 cells at a different time interval (2, 24 and 48 hrs)

Values are expressed as the Mean±Standard Deviation (SD). Different letters show the significantly different (p<0.05)

Image for - Enhanced Bioavailability of Epigallocatechin Gallate (EGCG) after Esterification and Complexation with Fish Oil

Fig. 5(a-b): Antiglycation activity (AGEs inhibitory activity)of various experimental samples like EGCG, EGCG-Ester and EGCG-Fish oil complex on C2BBe1 cells using (a) BSA-MGO model and (b) BSA-Fructose

Data are expressed as the Mean±Standard deviation (n = 3). Different letters show the significantly different (p<0.05). Aminoguanidine-positive control

Meanwhile, EGCG-ester showed better antiglycation activity than EGCG-Fish oil complex and thus hinting that EGCG-ester has few free OH groups that might influence the MGO trapping property, which results in good antiglycation activity.

DISCUSSION

The present study was framed to check the beneficial efficacy of esterified EGCG with fish oil (holistic effect) by assessing the esterification efficiency and permeability ability (bioavailability of EGCG). Followed by exploring the oxidative capacity and anti-glycation activity on C2BBe1 cells by comparing with EGCG and EGCG-Fish oil complex. The major reason for this esterification is to improve EGCG bioavailability and thereby its impact on various biological functions. The esterification/complexation of EGCG with fish oil (DHA/EPA) was confirmed by the results of the TLC, 13CNMR and GC-MS technique. During the esterification process, the hydroxyl group of EGCG will bind to the acryl group of different fatty acids (acylation reaction) like DHA and EPA and finally form an EGCG-ester (EGCG-DHA or EPA). Also, during this study by NMR analysis, few free hydroxyl groups in EGCG-ester (data not shown) were confirmed and thus retaining its antioxidant activity. NMR and MS spectrum results were similar to the outcome of Zhong and Shahidi16 studies, where different EGCG ester derivative is observed in both NMR and GC-MS spectrum.

The antioxidant status of different samples (EGCG, EGCG-Esters and EGCG-Fish oil) on C2BBel cells were quantified by TEAC and DPPH scavenging activity. Both the TEAC and DPPH scavenging activity was significantly higher in EGCG-ester (DHA/EPA) group as compared with EGCG and EGCG-fish oil complex group. Similarly, Zhong and others4 demonstrated that EGCG-fatty acid ester showed potent antioxidant activity due to increased lipophilicity (improve bio accessibility)and by facilitating the hydrogen atom donating property (due to increased acylation process). Moreover, oxidative stability index (OSI) was higher in EGCG-ester (9.87 hrs) than EGCG-Fish oil complex (8.2 hrs). In addition as mentioned previously that still, the EGCG-ester has few free hydroxyl groups, which might also contribute to better antioxidant activity with improved bioavailability.

For the current cell line study, we preferred human colon epithelial adenocarcinoma C2BBe1 cells (clonal of Caco2 cells) to check the EGCG bioavailability (cellular uptake) as C2BBe1 colon cells mimic the human intestinal environment23.Before, check the EGCG bioavailability, the author would first like to check the cytotoxicity or proliferation property of different samples (different concentrations at various time intervals) on C2BBe1 cells. The outcome of the MTT assay showed that C2BBe1 cells cultured with different concentrations of EGCG, EGCG-Ester and EGCG-Fish oil complex did not infer any significant changes in the cell number (viability) or proliferation rate at 24 and 48 hrs. Hence, showcasing that all the samples (EGCG, EGCG-Ester and EGCG-Fish oil complex) are safe, even at higher concentrations. Furthermore, C2BBe1 cells treated with EGCG, EGCG-Ester and EGCG-Fish oil complex, did not show any morphological changes. Based on the above results, the author has confirmed that none of the experimental samples could induce toxicity or cell death, even at higher concentrations. Previously, Mori and his colleagues24 also indicated that EGCG- fatty acid ester derivatives did not show any difference in cell viability using MTT assay and thus concluded that EGCG and its fatty acid derivatives are safe.

Aforementioned that EGCG is highly hydrophilic in nature and sensitive towards pH, light, temperature and its high reactivity property (especially with digestive enzymes) which makes EGCG more unstable and easily oxidizable molecule. Hence, EGCG bioavailability is significantly hampered to overcome this issue, many researchers started to esterify EGCG with the different molecules to improve the bioavailability3,5,6. The EGCG cellular uptake efficiency of each sample was calculated based on the permeability coefficient as mentioned before. The EGCG cellular uptake (permeability coefficient) was higher in the EGCG-ester group as compared to EGCG alone or EGCG-Fish oil complex group. The author speculates that during esterification (EGCG-ester), process the lipophilicity and steric property of EGCG-ester were considerably increased. That might enhance the EGCG-ester stability and superior cellular affinity (plasma membrane) and thus increase the EGCG permeability or cell uptake. Zhong and his colleagues4 also demonstrated that EGCG ester derivatives (lipophilized EGCG) showed greater cellular uptake or absorption than parent EGCG.

AGEs are the end products produced by the non-enzymatic reaction between reducing sugars and amino groups of proteins, nucleic acids, lipids (Amadori rearrangement). AGEs were considered as a pathogenic factor linking Diabetic Mellitus and Cardiovascular Disease as they trigger inflammation and oxidative stress25,26. BSA-MGO and fructose model tests are the standard method to check the anti-glycation or anti-AGEs activity of any drug27. For the current study, the anti-glycation activity of EGCG, EGCG-ester and EGCG-Fish oil complex was examined by including a standard aminoguanidine (trap MGO) in C2BBc1 cells. C2BBel cells treated with EGCG, EGCG-Esters and EGCG-Fish oil slightly lowered the AGEs production by trapping MGO and fructose (avoid MGO-BSA glycation). However, EGCG display greater AGEs inhibition activity in both MGO and fructose models than

EGCG-Esters and EGCG-Fish oil complex groups. Since EGCG alone group has many free hydroxyl groups which might effectively trap MGO and form adduct and thus lower AGES production. But, the esterified EGCG and EGCG-fish oil complex has limited free hydroxyl groups. Thus, demonstrating lower AGEs inhibitor activity than the EGCG group. Nevertheless, EGCG-ester showed better antiglycation activity than EGCG-Fish oil complex and thus hinting that EGCG-ester has few free OH groups (contribute to antioxidant activity) that might influence the MGO trapping property, which results in good antiglycation activity. In agreement with our results, Wang and his co-workers28 hinted that the EGCG-fatty acid ester derivatives especially EGCG-DHA/EPA showed higher antioxidant and MGO trapping activity due to higher lipophilicity, improved bioavailability and stability makes EGCG-esters a better anti-glycation agent than EGCG-Fish oil complex. In addition, the EGCG-ester displays better α-glucosidase activity than EGCG or EGCG-Fish oil complex and thus conferring its antiglycation property. The major strength of this study was to compare the bioavailability efficiency and beneficial effect (antioxidant and antiglycation activities) of EGCG-ester with EGCG-fish oil complex and parent EGCG. Because of limitations, current study lacks the detailed structural, physical and chemical properties elucidation of various EGCG ester derivatives. Hence, extensive pharmacokinetic and dynamic studies should be conducted with different EGCG ester derivatives.

CONCLUSION

Present study demonstrated the beneficial efficacy of EGCG esters and EGCG-Fish oil complex through improving antioxidant capacity (better TEAC and DPPH scavenging activity), followed by increased EGCG permeability (bioavailability of EGCG) and anti-glycation activity (MGO and Fructose induced AGEs inhibitory activity) on C2BBe1 cells. Overall, EGCG-ester showed superior antioxidant and antiglycation activity owing to enhance lipophilicity, steric effect and electron/nucleophilic donor capacity. Further, pharmacokinetic and dynamics studies are needed to elucidate the structural modification undergone during esterification/complexation. Also, animal studies (toxicity and dose fixation) and human trials are needed to check the real bioavailability efficacy of EGCG esters.

SIGNIFICANCE STATEMENT

Current study indicates that EGCG-ester showed superior EGCG bioavailability, antioxidant and antiglycation activity than EGCG-fish oil complex and EGCG. This novel combination of EGCG with fish oil (esterification/complexation) would significantly improve various beneficial effect of EGCG due to high lipophilicity and increase electron donor capacity. Hence, EGCG-fatty acid ester would be commercially developed in large scale and might be used to improve overall health status. However, further animal and human trial are need to confirm its beneficial efficacy.

Image for - Enhanced Bioavailability of Epigallocatechin Gallate (EGCG) after Esterification and Complexation with Fish Oil

Fig. S1: Thin Layer Chromatography (TLC) of fish oil and its isolates (free fatty acids- EPA/DHA)

Image for - Enhanced Bioavailability of Epigallocatechin Gallate (EGCG) after Esterification and Complexation with Fish Oil Fig. S2(a-c): Morphological changes on C2BBe1 cells treated with EGCG (A), EGCG ester (B), EGCG-Fish oil complex (C)

REFERENCES

  1. Venkatakrishnan, K., H.F. Chiu and C.K. Wang, 2019. Extensive review of popular functional foods and nutraceuticals against obesity and its related complications with a special focus on randomized clinical trials. Food Funct., 10: 2313-2329.
    CrossRefDirect Link
  2. Chiua, H.F., Y.C. Shen, K. Venkatakrishnanc and C.K. Wang, 2018. Popular functional foods and nutraceuticals with lipid lowering activity and in relation to cardiovascular disease, dyslipidemia and related complications: An overview. J. Food Bioactives, 2: 16-27.
    CrossRefDirect Link
  3. Han, Y.C., H.F. Chiu, Y.T. Ho, K. Venkatakrishnan and C.K. Wang, 2020. Improved bioavailability of EGCG after complexation with royal jelly protein. J. Food Biochem., Vol. 44.
    CrossRefDirect Link
  4. Zhong, Y., C.M. Ma and F. Shahidi, 2012. Antioxidant and antiviral activities of lipophilic epigallocatechin gallate (EGCG) derivatives. J. Funct. Foods, 4: 87-93.
    CrossRefDirect Link
  5. Cai, Z.Y., X.M. Li, J.P. Liang, L.P. Xiang and K.R. Wang et al., 2018. Bioavailability of tea catechins and its improvement. Molecules, Vol. 23.
    CrossRefDirect Link
  6. Ramesh, N. and A.K.A. Mandal, 2019. Pharmacokinetic, toxicokinetic, and bioavailability studies of epigallocatechin-3-gallate loaded solid lipid nanoparticle in rat model. Drug Dev. Ind. Pharm., 45: 1506-1514.
    CrossRefDirect Link
  7. Mereles, D. and W. Hunstein, 2011. Epigallocatechin-3-gallate (EGCG) for clinical trials: more pitfalls than promises? Int. J. Mol. Sci., 12: 5592-5603.
    CrossRefDirect Link
  8. Lee, M.J., P. Maliakal, L. Chen, X. Meng and F.Y. Bondoc et al., 2002. Pharmacokinetics of tea catechins after ingestion of green tea and (-)-epigallocatechin-3-gallate by humans: Formation of different metabolites and individual variability. Cancer Epidemiol. Biomarker Prev., 11: 1025-1032.
    Direct Link
  9. Chiu, H.F., K. Venkatakrishnan, O. Golovinskaia and C.K. Wang, 2021. Gastroprotective effects of polyphenols against various gastro-intestinal disorders: A mini-review with special focus on clinical evidence. Molecules, Vol. 26.
    CrossRefDirect Link
  10. Zagury, Y., M. Kazir and Y.D. Livney, 2019. Improved antioxidant activity, bioaccessibility and bioavailability of EGCG by delivery in β-lactoglobulin particles. J. Funct. Foods, 52: 121-130.
    CrossRefDirect Link
  11. Liang, J., H. Yan, P. Puligundla, X. Gao, Y. Zhou and X. Wan, 2017. Applications of chitosan nanoparticles to enhance absorption and bioavailability of tea polyphenols: A review. Food Hydrocolloids, 69: 286-292.
    CrossRefDirect Link
  12. Innes, J.K. and P.C. Calder, 2018. The differential effects of eicosapentaenoic acid and docosahexaenoic acid on cardiometabolic risk factors: A systematic review. Int. J. Mol. Sci., Vol. 19.
    CrossRefDirect Link
  13. Narayan, B., K. Miyashita and M. Hosakawa, 2006. Physiological effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)—a review. Food Rev. Int., 22: 291-307.
    CrossRefDirect Link
  14. Alhassan, A., J. Young, M.E.J. Lean and J. Lara, 2017. Consumption of fish and vascular risk factors: A systematic review and meta-analysis of intervention studies. Atherosclerosis, 266: 87-94.
    CrossRefDirect Link
  15. Gao, H., T. Geng, T. Huang and Q. Zhao, 2017. Fish oil supplementation and insulin sensitivity: A systematic review and meta-analysis. Lipids Health Dis., Vol. 16.
    CrossRefDirect Link
  16. Zhong, Y. and F. Shahidi, 2011. Lipophilized epigallocatechin gallate (EGCG) derivatives as novel antioxidants. J. Agric. Food Chem., 59: 6526-6533.
    CrossRefDirect Link
  17. Giunta, B., H. Hou, Y. Zhu, J. Salemi, A. Ruscin, R.D. Shytle and J. Tan, 2010. Fish oil enhances anti-amyloidogenic properties of green tea EGCG in TG2576 mice. Neurosci. Lett., 471: 134-138.
    CrossRefDirect Link
  18. Sekhon-Loodu, S. and H.P.V. Rupasinghe, 2015. Docosahexaenoic acid ester of phloridzin inhibit lipopolysaccharide-induced inflammation in THP-1 differentiated macrophages. Int. Immunopharmacol., 25: 199-206.
    CrossRef
  19. Arnao, M.B., J.L. Casas, J.A. del Río, M. Acosta and F. García-Cánovas, 1990. An enzymatic colorimetric method for measuring naringin using 2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) in the presence of peroxidase. Anal. Biochem., 185: 335-338.
    CrossRefDirect Link
  20. Shimada, K., K. Fujikawa, K. Yahara and T. Nakamura, 1992. Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. J. Agric. Food Chem., 40: 945-948.
    CrossRefDirect Link
  21. Wang, W., Y. Yagiz, T.J. Buran, C.D.N. Nunes and L. Gu, 2011. Phytochemicals from berries and grapes inhibited the formation of advanced glycation end-products by scavenging reactive carbonyls. Food Res. Int., 44: 2666-2673.
    CrossRef
  22. Shen, Y., Z. Xu and Z. Sheng, 2017. Ability of resveratrol to inhibit advanced glycation end product formation and carbohydrate-hydrolyzing enzyme activity, and to conjugate methylglyoxal. Food Chem., 216: 153-160.
    CrossRefDirect Link
  23. Motlekar, N.A., K.S. Srivenugopal, M.S. Wachtel and B.B.C. Youan, 2006. Evaluation of the oral bioavailability of low molecular weight heparin formulated with glycyrrhetinic acid as permeation enhancer. Drug Dev. Res., 67: 166-174.
    CrossRefDirect Link
  24. Mori, S., S. Miyake, T. Kobe, T. Nakaya, S.D. Fuller, N. Kato and K. Kaihatsu, 2008. Enhanced anti-influenza a virus activity of (−)-epigallocatechin-3-o-gallate fatty acid monoester derivatives: Effect of alkyl chain length. Bioorg. Medic. Chem. Lett., 18: 4249-4252.
    CrossRefDirect Link
  25. Goldin, A., J.A. Beckman, A.M. Schimdt and M.A. Creager, 2006. Advanced glycation end products: Sparking the development of diabetic vascular injury. Circulation, 114: 597-605.
    CrossRefDirect Link
  26. Zhou, Q., K.W. Cheng, J. Gong, E.T.S. Li and M. Wang, 2019. Apigenin and its methylglyoxal-adduct inhibit advanced glycation end products-induced oxidative stress and inflammation in endothelial cells. Biochem. Pharmacol., 166: 231-241.
    CrossRefDirect Link
  27. Yamagishi, S.I. and T. Matsui, 2010. Advanced glycation end products, oxidative stress and diabetic nephropathy. Oxidative Med. Cell. Longevity, 3: 101-108.
    CrossRefDirect Link
  28. Wang, M., X. Zhang, Y.J. Zhong, N. Perera and F. Shahidi, 2016. Antiglycation activity of lipophilized epigallocatechin gallate (EGCG) derivatives. Food Chem., 190: 1022-1026.
    CrossRefDirect Link

Other Health Sciences Concepts:

[back to top]

Discover the significance of concepts within the article: ‘Enhanced Bioavailability of Epigallocatechin Gallate (EGCG) after...’. Further sources in the context of Health Sciences might help you critically compare this page with similair documents:

Discussion, Absorption, Fish oil, Protein, Conclusion, Half life, Statistical data, Bioavailability, Spss software, Toxicity studies, P Value, Anti-inflammatory, One-way ANOVA, Cardiovascular disease, Piperine, Curcumin, Anti-microbial properties, Antioxidant capacity, TLC, Diabetic mellitus, Animal studies, In vitro studies, Cell viability, Antioxidant, Ascorbic acid, Table 1, Dose fixation, Nutraceutical, Anticancer, Systemic circulation, Cytotoxicity, Antioxidant status, Green tea, Epigallocatechin gallate (EGCG), Overall Health Status, HPLC, Reducing sugar, Holistic effect, Anti diabetic, Cytotoxicity activity, GC-MS, NMR spectroscopy, Permeability Coefficient, Anti Hypertensive, Catechin, Pharmacokinetic studies, Omega-3 fatty acid, Sodium bicarbonate, Low bioavailability, HPLC grade, Anti-microbial, Complexation, Structural Modification, Pharmacodynamic studies, Antiglycation activity, Acylation, Cell line, Compound microscope, Fetal Bovine Serum (FBS), Lipophilicity, High lipophilicity, Plasma membrane, Aminoguanidine, Esterification, Sonicator, Pharmacokinetics Studies, Amadori rearrangement, Advanced glycation end products (AGEs), DPPH scavenging activities, Docosahexaenoic acid (DHA), Low water solubility, Antihyperlipidemic, Morphology Analysis, Bioaccessibility, Dulbecco's modified Eagle medium (DMEM), 13CNMR, Alpha glucosidase activity, L-glutamine, Free hydroxyl group, Anti-obesity, Human trial, Fatty acid ester, CO2 incubator, Oxidative capacity, Fig. 1.

Let's grow together!

I humbly request your help to keep doing what I do best: provide the world with unbiased sources, definitions and images. Your donation direclty influences the quality and quantity of knowledge, wisdom and spiritual insight the world is exposed to.

Let's make the world a better place together!

Like what you read? Help to become even better: