Significance of Well diffusion method
The Well diffusion method is a laboratory technique used to evaluate the antimicrobial activity of substances, including plant extracts, by measuring the zone of inhibition around wells created in agar plates. This method allows researchers to assess the effectiveness of antibacterial and antifungal agents by observing how they inhibit microbial growth. By placing test substances in wells and measuring the resulting inhibition zones, the technique provides crucial data on the antimicrobial properties of diverse compounds against various microorganisms.
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The concept of Well diffusion method in scientific sources
The Well diffusion method is a laboratory technique employed to evaluate the antibacterial and antimicrobial activity of substances by observing the inhibition zones formed around samples on agar plates, indicating the extent of their effectiveness.
From: The Malaysian Journal of Medical Sciences
(1) A technique used to measure the efficacy of antifungal agents by creating wells in agar plates where specific concentrations of agents are placed to observe zones of inhibition against Candida strains.[1]