Significance of Thin Layer Chromatography
Thin Layer Chromatography (TLC) is a widely used analytical technique. It separates and identifies compounds in a mixture based on their different migration rates on a thin layer of absorbent material, often a silica gel plate. TLC is used to assess the quality, purity, and chemical composition of various substances, including plant extracts, herbal formulations, and oils. It is employed to monitor reactions, identify active principles, and compare samples, with advantages including adaptability, speed, and cost-effectiveness.
Synonyms: Tlc, Planar chromatography, Two-dimensional chromatography, Thin-film chromatography, Chromatographic technique, Adsorption chromatography
The below excerpts are indicatory and do represent direct quotations or translations. It is your responsibility to fact check each reference.
Hindu concept of 'Thin Layer Chromatography'
In Hinduism, Thin Layer Chromatography (TLC) is an analytical technique used to assess herbal medicine samples, such as Pippalyasava, Chitraka extracts, and others. It helps identify and separate chemical compounds, assess purity, and create profiles of their constituents. TLC aids in quality control and authentication of botanical materials.
From: Journal of Ayurveda and Integrated Medical Sciences
(1) Thin Layer Chromatography, or TLC, is used to identify and ensure the purity of a drug by comparing it with standard references, which is essential in the standardization of Tribhuvanakirti Rasa.[1] (2) It is a technique used to separate and identify chemical compounds in a drug sample.[2] (3) This is a method employed to analyze the sample, with the results of this method presented in a figure, and the sample showed a certain number of bands.[3] (4) The method used to separate and identify the components of the plant extract, which was carried out in Sri Sri Tattava, Bangalore.[4] (5) This is a chromatographic technique used to separate and identify the components of Mocharasa, based on their different polarities and affinities, and is used to create a profile of its chemical constituents.[5]
From: International Research Journal of Ayurveda and Yoga
(1) This is a chromatographic technique used to separate components of a substance on a thin layer of adsorbent.[6] (2) This is a method used in the preliminary analysis of various functional groups present in Acacia catechu.[7] (3) This was performed on the methanolic extract of the root of B. diffusa Linn. and was mentioned in the Ayurvedic Pharmacopoeia of India.[8] (4) Results of Thin layer Chromatography of a specific Ayurveda formulation are shown in table 4.[9] (5) This is a study that is done of Karavellaka Phala.[10]
From: Journal of Ayurvedic and Herbal Medicine
(1) This is a simple cheap technique used to establish the fingerprint of a herbal product.[11] (2) This is a technique used to develop a fingerprint of Thoravature Taila, using a mixture of ethyl acetate, methanol, cyclohexane and dichloromethane in a specific ratio.[12] (3) This is a technique employed to separate and analyze the components of the ethanol extracts from the five Balanophora species, based on their different polarities.[13] (4) This is a separation technique used to analyze the extracted oil, and it was used to separate and identify different components, with linoleic acid being used as a standard for comparison.[14] (5) This is a technique used to determine and separate the various chemical components present in a test sample of Prajasthapana mahakashya granules.[15]
The concept of Thin Layer Chromatography in scientific sources
Thin Layer Chromatography (TLC) is a versatile laboratory technique for separating and identifying compounds. It's used to analyze mixtures, assess purity, monitor reactions, and identify substances in extracts. TLC helps determine the presence of specific compounds and analyze various substances, including plant extracts and synthesized products.
From: International Journal of Pharmacology
(1) It is a technique used to separate and identify different compounds in a mixture, with silica gel plates used as the stationary phase.[16] (2) It is a technique used to separate various fractions from the crude extract, allowing for the detection of different phytochemicals like tannins, saponins, flavonoids, and phenols.[17] (3) Thin-Layer Chromatography (TLC) were performed on silica gel 60 pre-coated plates, (F-254 (Merck).[18]
From: The Malaysian Journal of Medical Sciences
(1) A technique used to identify Tagitinin C as a marker compound in extracts, followed by quantitative analysis with a densitometer.[19] (2) The analytical technique used to determine the purity and identity of the kolaviron compound, as described in the study.[20] (3) A method for separating chemical compounds in a mixture, used here to analyze the biocompounds extracted from Dendrocalamus asper.[21]
From: Journal of Medicinal Plants for Economic Development
(1) Thin Layer Chromatography is a technique used to analyze the chemical constituents of plant extracts, helping to identify compounds responsible for antioxidant and antifungal activities effectively.[22] (2) This is a technique used to separate compounds in a mixture, often used for analysis and purification.[23] (3) This is a technique discussed in the text, with the mention of apparatus and general techniques used in this process.[24]
From: Onderstepoort Journal of Veterinary Research
(1) A technique used to separate and identify different components in a mixture.[25] (2) The method is used for screening the fractions of the decoctions to identify the presence of various compounds, which is a key technique.[26]
From: Asian Journal of Pharmaceutics
(1) An analytical method used for simultaneous estimation and validation of vardenafil and dapoxetine hydrochloride.[27] (2) High-performance this profile represented in Figures 1-3 that showed 5 spots at 254 nm, 4 spots at 366 nm, and 8 spots at 540 nm, respectively.[28] (3) This method was used for interaction study which was conducted on medicated TDDS for mutation by comparing them with the pure drug and placebo formulation.[29]
From: Sustainability Journal (MDPI)
(1) Thin-layer chromatography (TLC) was employed to assess the mobility of procymidone in soil, separating it based on its movement through a soil-coated plate.[30] (2) Thin Layer chromatography, or TLC, is the analytical technique employed to monitor the progress of the reaction mixture until the complete consumption of the initial starting materials was observed after stirring for 24 hours.[31] (3) Thin-layer chromatography is an analytical technique used for separating and identifying components of a mixture, applied for meloxicam and piroxicam identification.[32]
From: International Journal of Environmental Research and Public Health (MDPI)
(1) A separation technique visualized in figures using an adsorbent like silica gel and various solvent systems, showing how different mobile phases affect the relative movement of the analyzed chemical constituents.[33] (2) The reactions were monitored using thin layer chromatography and gas chromatography mass spectroscopy chromatographic methods until reaction completion.[34] (3) Thin Layer Chromatography, also known as TLC, is a technique used in agave syrup analysis to show specific sugar profiles, mainly composed of fructose and fructo-oligosaccharides, when compared to other tested syrups.[35]
From: International Journal of Pharmacology
(1) Thin layer chromatography on silica gels was used to confirm the purity of Barakol.[36] (2) Thin-layer chromatography provides a simplified method for identifying aerobic actinomycetes.[37] (3) Thin Layer Chromatography (TLC) is a common laboratory procedure used to qualitatively measure compounds by separating them based on their differing Rf values.[38]