Significance of RT-PCR
RT-PCR, or Reverse Transcription Polymerase Chain Reaction, is a versatile molecular biology technique with various applications. It is frequently employed to detect and quantify gene expression by analyzing RNA levels. This technique is used in diagnosing diseases like COVID-19, measuring the presence of specific viruses, and assessing the expression levels of genes in different tissues. RT-PCR involves converting RNA into DNA, amplifying it, and then analyzing it to determine gene expression or the presence of a virus.
Synonyms: Real-time polymerase chain reaction, Quantitative pcr, Qpcr, Reverse transcription polymerase chain reaction, Reverse transcriptase pcr, Real-time pcr, Reverse transcription quantitative pcr, Qrt-pcr.
The below excerpts are indicatory and do represent direct quotations or translations. It is your responsibility to fact check each reference.
Hindu concept of 'RT-PCR'
In Hinduism, RT-PCR is a diagnostic tool for Covid-19. It confirms the virus's presence, aiding patient assessment. It was also used in a clinical study to evaluate Ayush-64's safety and efficacy.
From: Journal of Ayurveda and Integrated Medical Sciences
(1) This is a test used to diagnose Covid-19, and it was used in a clinical study to evaluate the safety and efficacy of Ayush-64.[1] (2) This is a test used to confirm the presence of the virus and is used to assess the patients.[2]
From: International Research Journal of Ayurveda and Yoga
(1) It is the gold standard for any patient with COVID-19 symptoms, and is an abbreviation for reverse transcriptase polymerase chain reaction.[3]
The concept of RT-PCR in scientific sources
RT-PCR, or Reverse Transcription Polymerase Chain Reaction, is a versatile molecular technique. It is used to amplify and analyze RNA, often to determine gene expression levels. It can be applied to various samples, including those for COVID-19 and other viral infections.
From: International Journal of Pharmacology
(1) A method used to assess the expression of MMP-3 and 13 genes.[4] (2) This was used to isolate RNA from separated lung tissue to determine gene expression.[5] (3) The expression of TNF-α and IL-6 were determined by real-time reverse transcription polymerase chain reaction.[6] (4) This was employed to evaluate the gene regulation of the ciprofloxacin derivative.[7] (5) The viral load of HCV was assessed as a result of HCV amplification by this.[8]
From: Onderstepoort Journal of Veterinary Research
(1) Reverse transcriptase PCR, known as RT-PCR, was performed to detect viral RNA, extracting it from nasal swabs using the RNeasy Mini Kit to confirm PPR in the surveyed animals in the Republic of Niger.[9] (2) It refers to a laboratory technique used for the detection of a certain virus and other similar viruses, involving reverse transcription and polymerase chain reaction.[10] (3) A method for detecting viral presence, however, immunohistochemical tests for FPLV antigen were not available to verify the histological or PCR results.[11] (4) It is a method used to identify goose paramyxovirus.[12] (5) Real-time reverse transcription-polymerase chain reaction tests are commonly used for IAV diagnosis purposes.[13]
From: The Malaysian Journal of Medical Sciences
(1) RT-PCR, or reverse transcriptase-polymerase chain reaction, is a molecular diagnosis method used to confirm a history of SARS-CoV-2 infection, with positive results indicating infected individuals in the included studies.[14] (2) The rapid development of the test kit reverse transcription polymerase chain reaction to detect the presence of SARS-CoV-2.[15] (3) An abbreviation for real-time polymerase chain reaction, a method used to determine the expression of vitamin D receptor mRNA.[16] (4) This is the abbreviation for reverse transcriptase polymerase chain reaction, a method used for genotyping rotavirus by analyzing the VP7 (G-type) and VP4 (P-type) genotypes.[17] (5) This is a method used to determine the expression of miR-3099 and various cellular markers at different time points during the differentiation process.[18]
From: Asian Journal of Pharmaceutics
(1) This real-time reverse transcription-polymerase chain reaction analyses was used to determine expression levels of caspase 8.[19] (2) Co-culture cells were treated and total RNA was isolated using the RNeasy Tissue Mini Kit from Qiagen according to the manufacturer’s instructions for this analysis.[20] (3) It is used to detect gene expression represented coproduction of the assigned enzymes encoding genes.[21] (4) This test is not mentioned in the text.[22] (5) It is a technique used to amplify and quantify RNA.[23]
From: Journal of Public Health in Africa
(1) It is a virological test used for large-scale screening to detect cases of infected people, including asymptomatic or pre-symptomatic individuals, enabling early treatment and reduced exposure.[24] (2) A laboratory technique used to detect viral RNA in air and environmental samples, indicating contamination.[25] (3) This is a real-time polymerase chain reaction test, which is the current gold standard for testing for SARS-CoV-2, as stated in the text.[26] (4) Reverse-transcriptase polymerase chain reaction test is used for the diagnosis of COVID-19 and is recommended for suspected COVID-19 patients when available.[27] (5) This is a technique used to analyze the dynamics of specific genes in the SARS-CoV-2 virus within a Moroccan population, as part of an epidemiological description and analysis conducted by Benrahma and colleagues, and the study was made available on medRxiv in 2020, on June 18th, with the identifier 2020.06.18.20135137.[28]
From: South African Family Practice
(1) Reverse transcriptase-polymerase chain reaction, a method of determining the levels of HIV RNA in plasma.[29]
From: International Journal of Environmental Research and Public Health (MDPI)
(1) It is a laboratory technique used for the detection of SARS-CoV-2 RNA, employed alongside serological tests to improve knowledge on infection epidemiologic spread.[30] (2) RT-PCR is a laboratory technique combining reverse transcription of RNA into DNA, and amplification of specific DNA targets using polymerase chain reaction to quantify gene expression where maternal SARS-CoV-2 infection was confirmed upon hospital admission by a real-time reverse transcriptase-polymerase chain reaction (RC-PCR) on a nasopharyngeal swab sample.[31] (3) RT-PCR was used to diagnose GZVI during pregnancy in cases where blood collection was performed until the fourth day of the clinic period.[32] (4) Real-time polymerase chain reaction, a laboratory technique used to detect the virus, and COVID-19 tests were performed on samples from combined nose and throat swabs by using the real-time polymerase chain reaction in accredited laboratories.[33] (5) This test is used to detect active infection, but it is not perfect, with a chance of false negatives, which means that infected people are not counted.[34]
From: Sustainability Journal (MDPI)
(1) RT-PCR is used for testing COVID-19 and high-affinity serological testing, according to a consensus statement from a panel of Latin American experts.[35] (2) Interestingly, in these cases, pulmonary involvement was relatively mild, and this for SARS-CoV-2 in cerebrospinal fluid was negative, providing support to the hypothesis of an aberrant immune response rather than direct viral invasion as the underlying mechanism.[36]
From: International Journal of Pharmacology
(1) Reverse transcription-polymerase chain reaction, a technique used to analyze the expression of genes like MEST.[37] (2) RT-PCR, or Reverse Transcription Polymerase Chain Reaction, was used to analyze the expression levels of specific genes, such as clock genes.[38] (3) RT-PCR is a method used to detect the expression of CIDE-B mRNA in hippocampal tissue.[39] (4) RT-PCR is a method used to assess gene expression levels, including those of Sox9, Col2a1, aggrecan, and Col10a1, after extracting total RNAs and converting them to complementary DNAs.[40] (5) Reverse Transcriptase-Polymerase Chain Reaction, a molecular biology technique used to analyze gene expression by measuring mRNA levels.[41]