Significance of Rf value

The Rf value, or retention factor, is a key measurement in chromatography, representing the ratio of the distance traveled by a compound to the distance traveled by the solvent front. This ratio is critical for characterizing compounds during chromatographic analysis, aiding in substance identification and differentiation. Rf values are determined from various chromatography techniques, including Thin Layer Chromatography (TLC) and High-Performance Thin-Layer Chromatography (HPTLC), and can vary for different compounds, providing insights into their movement and properties.

Synonyms: Retention factor, Separation factor

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The concept of Rf value in scientific sources