Significance of Reverse phase HPLC
Reverse phase HPLC is a chromatographic technique widely used for separating and analyzing compounds, particularly effective in pharmaceutical applications. It involves a non-polar stationary phase and a polar mobile phase, allowing the separation of non-polar compounds based on their hydrophobicity. This method is essential for validating drugs like Bedaquiline and estimating compounds such as Valsartan and Abrocitinib. Reverse phase HPLC is noted for its accuracy and is integral in routine measurements, including the analysis of ivermectin in various mixtures.
Synonyms: Rp-hplc, Reversed-phase chromatography, Reverse-phase liquid chromatography, Reverse-phase high-performance liquid chromatography
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The concept of Reverse phase HPLC in scientific sources
Reverse phase HPLC is a chromatography technique characterized by a nonpolar stationary phase and a polar mobile phase, enabling the effective separation of various compounds within a mixture.
From: World Journal of Pharmaceutical Research
(1) Reverse phase HPLC is the type of chromatography used in this study to facilitate the separation and analysis of multiple compounds.[1] (2) A type of chromatography used to separate the polyphenolic compounds in the leaf extract of Euphorbia cotinifolia.[2] (3) A method where separation is based on a non-polar stationary phase and a polar mobile phase.[3] (4) A chromatographic technique used for the estimation of compounds, specifically here for Abrocitinib, involving a mobile phase and stationary phase interaction.[4] (5) A chromatographic technique developed for the routine measurement of ivermectin in laboratory-prepared mixtures and combination dose forms.[5]