Significance of Retention time

Retention time is a crucial concept in chromatography, referring to the duration a compound takes to pass through a chromatography column and reach the detector. This time is vital for analyzing and identifying compounds during HPLC analysis, as it reflects the interaction between the analyte and the stationary phase. Specific retention times are recorded for various substances, influencing identification, quantification, and method validation. Understanding retention time aids in determining the presence and amount of specific analytes effectively.

Synonyms: Storage time, Persistence, Lag time, Dwell time, Residence time

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The concept of Retention time in scientific sources

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