Significance of Protein identification
Protein identification is a process that utilizes mass-spectrometry and proteolytic cleavage to analyze and verify the proteins found in a sample. This technique allows scientists to accurately determine the proteins present, aiding in various research and diagnostic applications. By breaking down proteins into smaller peptides, mass-spectrometry can provide detailed insights into the composition of biological samples, making protein identification a crucial part of proteomics.
Synonyms: Protein characterization, Protein analysis, Protein profiling, Protein detection
The below excerpts are indicatory and do represent direct quotations or translations. It is your responsibility to fact check each reference.
The concept of Protein identification in scientific sources
Protein identification involves utilizing mass spectrometry and proteolytic cleavage to thoroughly analyze and confirm the proteins present in a sample, ensuring accurate assessments of protein composition in various biological contexts.
From: Asian Journal of Pharmaceutics
(1) Sonication was employed to remove non-protein impurities, which enhanced mass spectrometry for this.[1] (2) This was done using MALDI-TOF MS and MASCOT software using Rattus specific taxonomy search.[2] (3) It is the process of determining the identity of proteins in a sample.[3]
From: The Malaysian Journal of Medical Sciences
(1) This is the process of determining the specific identity of proteins, often through mass spectrometry, providing information about their composition.[4] (2) Protein identification involves using mass-spectrometry alongside proteolytic cleavage to analyze and validate the proteins present in a sample.[5]
From: Onderstepoort Journal of Veterinary Research
(1) Protein identification Prepared samples were submitted to the Facility for Genomics and Proteomics at the University of the Free State for identification by nanoscale liquid chromatography coupled to tandem MS (Nano LC-MS/MS).[6] (2) Protein identification is the process of determining the identity of a protein, and protein bands of interest were trypsin digested as per the protocol described by Shevchenko et al. (2007).[7]
From: International Journal of Environmental Research and Public Health (MDPI)
(1) Protein identification is the process of determining the identity of proteins in a sample, and it was performed using mass spectrometry data and the Proteome Discoverer platform.[8] (2) **Protein identification** is enhanced by the high sensitivity of LC-ESI-MS/MS, which achieves effective peptide concentration and independent sequencing of peptides.[9]