Significance of Polymerase chain reaction
Polymerase chain reaction (PCR) is a versatile laboratory technique used extensively in various scientific fields. It serves to amplify specific DNA sequences, enabling the detection, analysis, and identification of genetic material. PCR is applied in numerous ways, including genotyping, gene expression analysis, pathogen detection, and disease diagnosis. The technique is essential for analyzing samples, identifying genetic variants, and confirming the presence of viruses or bacteria.
Synonyms: Pcr, Dna amplification, Molecular cloning, Amplification, Dna replication
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The concept of Polymerase chain reaction in scientific sources
Polymerase chain reaction (PCR) is a laboratory technique used to amplify specific DNA sequences. It's crucial for various analyses, including pathogen detection, genetic variation studies, and diagnosing infections. PCR is also used to analyze gene expression, detect viruses, and identify specific strains of organisms.
From: Onderstepoort Journal of Veterinary Research
(1) It is a technique used to amplify specific DNA sequences.[1] (2) Polymerase chain reaction provides a sensitive and specific detection of RVFV in cell culture and directly in serum samples from infected humans and animals.[2] (3) Polymerase chain reaction, or PCR, products indicated the presence of sheep-associated form for malignant catarrhal fever infection in Turkey, according to the nucleotide sequencing.[3] (4) This (PCR) and SRA gene probes were used to test for the presence of T. b.rhodesiense in two dogs from the two sleeping sickness focus of Kenya.[4] (5) It is a laboratory procedure employed to amplify deoxyribonucleic acid sequences, particularly in the detection of Ehrlichia ruminantium in Amblyomma variegatum ticks.[5]
From: The Malaysian Journal of Medical Sciences
(1) Polymerase chain reaction is one of the several types of HPV DNA tests available, including Southern blots, dot blot, in situ hybridization (ISH), and solution hybridization.[6] (2) RNA Isolation, cDNA Synthesis and Reverse Transcription-this process were performed in the study.[7] (3) It is a laboratory technique used to amplify specific DNA sequences for analysis.[8] (4) This is a method used to amplify specific sections of DNA, allowing for detailed genetic analysis of the p53 and p27 genes.[9] (5) This is a molecular test that was not available for the study, and it could have been used to further analyze the samples from the children.[10]
From: Asian Journal of Pharmaceutics
(1) The species confirmation of the Campylobacter spp., isolated cultures was also performed using this (PCR).[11] (2) This amplification is comparable to this (PCR) amplification of nucleotide sequences, and can theoretically be used to detect hundreds of protein targets at a time in patient samples.[12] (3) In this protocol 16 S rRNA Universal primers, gene fragment was amplified.[13] (4) This is a laboratory technique used to amplify specific DNA sequences for detection.[14] (5) This method is promising for detection of polymorphic sites of genes of isolates.[15]
From: South African Journal of HIV Medicine
(1) The polymerase chain reaction (PCR) for detecting the DNA proviral form of the virus integrated into the genome of peripheral blood cells is the established laboratory investigation of choice for the diagnosis of perinatally acquired HIV-1 infection.[16] (2) Polymerase chain reaction methods are highly sensitive techniques used in early infant diagnosis of HIV, and these methods, coupled with rapid linkage to treatment, represent the gold standard in pediatric HIV care.[17] (3) Polymerase chain reaction was the method used to diagnose infections such as Trichomonas vaginalis, Chlamydia trachomatis, Neisseria gonorrhoeae and Mycoplasma genitalium from vaginal specimens.[18] (4) The introduction of universal PCR testing at birth would simplify the issue and allow focus to be solely on exposures.[19] (5) A molecular biology technique used to amplify and detect specific DNA sequences, confirming the presence of herpes simplex virus type 1 in the endotracheal aspirate and blood samples.[20]
From: International Journal of Pharmacology
(1) It was run in a Mini Cycler for 35 cycles, with specific primers of each GAT subtypes used.[21] (2) PCR amplification was used with specific primers to analyze the genotypes of the subjects.[22] (3) A method used to detect the DNA of HBV in serum of some patients with ALF despite testing negative for hepatitis B surface antigen.[23] (4) Afterwards the reaction tubes containing RT preparations were flash-cooled in an ice chamber until being used for DNA amplification through this.[24] (5) This procedure was conducted in a micro-amplification tube for genomic DNA from P. aeruginosa.[25]
From: Journal of Public Health in Africa
(1) A method used to test samples collected from bats in the Mubende area, though the results were negative for Ebola virus disease.[26] (2) It is an advanced technique for cholera testing, and decentralizing its testing has been a challenge for countries.[27] (3) All DNA extraction and this steps were performed in a UV light-decontaminated laminar flow hood.[28] (4) This was used to detect intestinal protozoa.[29] (5) This is a highly sensitive and specific method for diagnosing herpes simplex virus infection, and the study did not have facility for this test.[30]
From: South African Family Practice
(1) Polymerase chain reaction, also known as PCR, is a technique needed to distinguish between malaria recrudescence and reinfection after 14 days.[31] (2) This describes the gold standard for the diagnosis of SARS-CoV-2 infection, and is a very sensitive molecular method of reverse transcribing SARS-CoV-2 RNA into DNA, followed by amplification of the target genomic sequence.[32] (3) This is a highly sensitive laboratory technique used to detect and amplify specific DNA sequences, useful for diagnosing HSV infections in various samples.[33]
From: Journal of Medicinal Plants for Economic Development
(1) This is a molecular biology technique used to amplify specific DNA regions, enabling the study of the genetic material and species identification.[34]
From: South African Journal of Physiotherapy
(1) This is a more accurate test that tests the infants leucocytes for the presence of HIV DNA and is not routinely used in South African hospitals.[35]
From: Journal of Metabolic Health
(1) Quantitative polymerase chain reaction provides the benefit of being able to use smaller amounts of DNA, thereby making it feasible to conduct epidemiology studies involving huge numbers of people.[36]
From: International Journal of Environmental Research and Public Health (MDPI)
(1) Polymerase Chain Reaction (PCR) species identification of members of the An. funestus group and the An. gambiae complex was conducted.[37] (2) Refers to a laboratory technique used to amplify DNA, which can be used to detect the presence of viral genomes in samples.[38] (3) A laboratory technique used for COVID-19 screening, required for patients before cataract surgery to ensure they are not infected.[39] (4) The number of confirmed positives by polymerase chain reaction virus testing by day was obtained from local governments and summarized for the analysis.[40] (5) Polymerase chain reaction is not very helpful for the diagnosis of West Nile Virus infections due to low and short viremia in patients, which makes it less reliable compared to serological methods.[41]
From: Sustainability Journal (MDPI)
(1) The polymerase chain reaction (PCR) was used to amplify bacterial 16S rDNA and fungal ITS-1 regions, enabling the quantitative analysis of microbial species’ presence and relative abundance.[42] (2) Polymerase chain reaction (PCR) was performed with universal primer sets of 18 S rRNA, rbcL , and ITS 2 regions.[43] (3) The polymerase chain reaction (PCR) amplifications were performed, recovered and quantified for each sample in accordance with the procedure described in a previous study.[44] (4) It is a method used to analyze the relationship of abundance with microscopy, with a specific slope, correlation coefficient, and equation.[45] (5) Polymerase chain reaction, or PCR, is a technique used to amplify specific DNA sequences, enabling the identification and analysis of microorganisms.[46]
From: International Journal of Pharmacology
(1) Polymerase Chain Reaction (PCR) is a laboratory technique used in the study to amplify specific DNA sequences for genotyping the PON1 gene, specifically for the Q192R and L55M polymorphisms.[47] (2) A laboratory technique used on ear samples to analyze gene expression changes related to Nrf2 activity and inflammation.[48]