Significance of PCR amplification
PCR amplification, as described by both Science and Health Sciences, is a vital laboratory technique used to create multiple copies of specific DNA segments. This process increases the quantity of a targeted DNA sequence, enabling researchers to analyze, identify, and detect genes, pathogens, and other genetic material. The technique relies on the polymerase chain reaction (PCR) to exponentially replicate the desired DNA segments, making it essential for various genetic analyses and molecular biology applications.
Synonyms: Polymerase chain reaction, Nucleic acid amplification., Dna amplification, Molecular cloning, Nucleic acid amplification
The below excerpts are indicatory and do represent direct quotations or translations. It is your responsibility to fact check each reference.
The concept of PCR amplification in scientific sources
PCR amplification is a lab technique used to create multiple copies of specific DNA segments. This process allows for detailed analysis, detection of specific genes (like icaAB, mec A, and antibiotic resistance genes), identification of mutations, and generation of DNA profiles for various analyses and experimentation.
From: The Malaysian Journal of Medical Sciences
(1) PCR amplification of the nucleotide sequence corresponding to the HspX gene was performed from the MTB H37Rv genome.[1] (2) The cDNA was used as a template for subsequent amplification.[2] (3) A method used to create multiple copies of a specific DNA sequence, enabling researchers to analyze the gut microbiota.[3] (4) This is a process of using polymerase chain reaction to create multiple copies of a specific DNA fragment.[4] (5) This is a process performed to amplify DNA segments of H-ras, K-ras and N-ras, as described in the methodology section of the text.[5]
From: Onderstepoort Journal of Veterinary Research
(1) This involved using a nested PCR protocol targeting the genomic ITS-1 region to detect each Eimeria species.[6] (2) This is the process of making multiple copies of a DNA sequence, which was used to detect and differentiate between Theileria species.[7] (3) This is a process used to amplify DNA, and in this study, the PCR amplification resulting from four parasitic samples at different developmental stages is presented.[8] (4) This is a technique used to amplify particular regions of deoxyribonucleic acid (DNA), which is used to identify and verify the presence of Mycobacterium avium subspecies paratuberculosis (MAP).[9] (5) A method employed to increase the quantity of specific DNA segments, used to detect the virus.[10]
From: International Journal of Pharmacology
(1) A process performed using specific reagents and thermal cycling conditions to amplify DNA for RAPD and ISSR analysis.[11] (2) This process of partial 16S rRNA gene of marine bacterium TASC.16 and monkey isolate K6.72 was performed according to the methods of Thiel and Imhoff (2003) and Salasia et al. (2001), respectively.[12] (3) This is used for confirmation of cDNA quality of GAPDH gene.[13] (4) A laboratory technique used to create multiple copies of DNA segments for gene sequencing.[14] (5) A technique used to exponentially amplify specific DNA sequences, essential for generating sufficient material for sequencing and analysis of the 16S rDNA gene.[15]
From: Asian Journal of Pharmaceutics
(1) A process carried out in a thermal cycler to amplify DNA.[16] (2) Genus identification for bacteria exhibiting oil-degrading activity was done by this.[17]
From: Journal of Medicinal Plants for Economic Development
(1) This is the process of using PCR to create multiple copies of a specific DNA sequence, requiring high-quality DNA.[18]
From: International Journal of Environmental Research and Public Health (MDPI)
(1) After the extraction, the samples were diluted to a concentration of 1 ng / L with sterile water, then the diluted genomic DNA was used as the template for this.[19] (2) PCR amplification of HPV DNA was performed using GoTaq ® Flexi DNA Polymerase and specific primers, enabling the replication of the target DNA region for sequencing.[20] (3) It is a technique used to amplify specific DNA sequences from the extracted DNA for sequencing.[21] (4) PCR amplification was performed using FastStart master mix from Roche to amplify the conserved 16S V3-V4 region, with specific forward and reverse primers under specific conditions.[22] (5) It is a technique used to create multiple copies of a specific DNA segment, enhancing the ability to detect and analyze microbial genes.[23]
From: Sustainability Journal (MDPI)
(1) PCR amplification experiment was used to detect the corresponding resistance gene (sul 1 , sul 2 , sul 3 ) and the internal control gene (16 S rRNA) of sulfa antibiotics.[24] (2) Universal primers targeting conserved regions of the 16 S rDNA were employed for PCR amplification, followed by paired-end sequencing of one or more hypervariable regions using the Illumina MiSeq/HiSeq platforms.[25] (3) It is a molecular technique used to amplify specific DNA regions, employed in both culture-dependent and culture-independent methods to analyze the soil microbiological community.[26] (4) A denaturing gradient gel electrophoresis analysis of the bacterial community in water samples (treated, untreated wastewater, and seawater) was conducted, and "PCR amplification" targeting the 16 S rDNA gene was performed.[27] (5) This is a technique used to create multiple copies of a specific DNA sequence, enabling the detection and analysis of bacteria in environmental samples.[28]
From: International Journal of Pharmacology
(1) PCR amplification was performed for both β-actin and eNOS.[29] (2) PCR amplification is a process used to increase the quantity of specific DNA sequences for analysis.[30] (3) A laboratory technique used to amplify specific segments of DNA, employed in this study to detect the presence of the mcr-1 gene.[31] (4) A technique used to amplify specific DNA sequences, such as the 16S rRNA gene, to obtain sufficient material for sequencing.[32] (5) This is the process of repeatedly copying a specific DNA segment using PCR, leading to an exponential increase in the target sequence.[33]