Significance of PCR amplification
PCR amplification, or Polymerase Chain Reaction, is a crucial molecular biology technique that replicates specific DNA sequences in vitro, producing millions of copies for further analysis or experimentation. This method enables the detection of various genetic materials, including antibiotic resistance genes, mutations, and specific microbial DNA sequences, making it essential for genetic research and diagnostics. It operates through a series of temperature cycles that facilitate the exponential replication of targeted DNA segments, assisting in identifying and confirming the presence of specific genes.
Synonyms: Polymerase chain reaction, Molecular cloning
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The concept of PCR amplification in scientific sources
PCR amplification is a critical laboratory technique employed to create multiple copies of specific DNA segments, facilitating the analysis of genes such as SMN2, ica AB, and mec A, and aiding genetic research across species.
From: World Journal of Pharmaceutical Research
(1) A method used to create copies of DNA segments, vital for the identification of microorganisms like Fusarium by examining specific genetic sequences.[1] (2) A laboratory technique used to amplify specific DNA sequences, essential for the identification of resistance genes in bacteria.[2] (3) A molecular biology technique used to amplify specific DNA sequences, such as the aroE gene, to detect its presence in bacterial isolates.[3] (4) The step in which specific DNA segments are duplicated through a series of temperature cycles.[4] (5) A technique used to make multiple copies of a specific DNA segment, allowing for detailed analysis and identification.[5]
From: The Malaysian Journal of Medical Sciences
(1) A method used to replicate specific DNA segments, making millions of copies of a particular DNA sequence, which is essential for detecting mutations.[6] (2) A laboratory technique used to replicate and amplify specific segments of DNA, allowing for the detection of HCV.[7] (3) PCR amplification is the method used to detect specific DNA sequences indicative of Leptospira species.[8] (4) A technique used to replicate a specific DNA sequence exponentially, resulting in a large enough quantity for analysis.[9]