Significance of Nicotinic receptor binding
Nicotinic receptor binding was assessed using a radioligand binding assay. The procedure involved incubating protein samples at a concentration of 400 μg mL –1 with 0.75 nM of [3H]-cytisine, a nicotinic receptor ligand. Various concentrations of test compounds were added along with Tris-HCl buffer to a final volume of 1 mL. The mixture was incubated for 75 minutes at 4°C to facilitate binding interactions.
Synonyms: Attachment, Affinity, Interaction, Connection, Adhesion, Cholinergic receptor binding
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The concept of Nicotinic receptor binding in scientific sources
Nicotinic receptor binding, as described, involves incubating protein samples with [3H]-cytisine and varying concentrations of test compounds in Tris-HCl buffer at 4°C for 75 minutes.
From: International Journal of Pharmacology
(1) For this, 400 μg mL –1 of protein-containing samples and 0.75 nM final concentrations of [ 3H]-cytisine were incubated with different concentrations of tested compounds and Tris-HCl buffer (1 mL final volume) for 75 min at 4°C.[1]