Significance of Multiplex polymerase chain reaction
Multiplex polymerase chain reaction (Multiplex PCR) is a laboratory technique that allows the simultaneous amplification of multiple specific DNA segments in a single reaction. This method facilitates the detection of various targets, such as respiratory viruses and virulence genes in bacterial isolates, including those from Shiga toxigenic Escherichia coli and Klebsiella pneumoniae. By enabling rapid and efficient detection, Multiplex PCR offers significant advantages over traditional methods in microbial analysis and other applications.
Synonyms: Multiplex pcr, Multiplex amplification
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The concept of Multiplex polymerase chain reaction in scientific sources
Multiplex polymerase chain reaction (Multiplex PCR) is a laboratory technique that amplifies multiple DNA fragments at once, aiding in the swift identification of virulence genes in bacterial pathogens like Klebsiella pneumoniae.
From: Onderstepoort Journal of Veterinary Research
(1) It is a PCR assay used for detection of enterotoxin genes and other virulence factors.[1] (2) This is a method used to identify the Trichinella larvae, and it was used to identify one larva as T. nelsoni and two as Trichinella T 8 in the study.[2] (3) This is a molecular biology technique, which was used to analyze the samples of weanling pigs, which allows for the detection of multiple pathogens simultaneously.[3] (4) This is a laboratory technique used to amplify and detect multiple DNA sequences simultaneously, often employed for the detection and characterization of Listeria monocytogenes strains.[4] (5) This is a technique used to detect multiple virulence genes simultaneously in the APEC isolates, and it was used in the study to screen for the presence of 12 virulence genes.[5]
From: The Malaysian Journal of Medical Sciences
(1) This refers to a specific laboratory technique employed to isolate and characterize Shiga toxigenic Escherichia coli that originate from animals and birds.[6] (2) Multiplex polymerase chain reaction is a method used in this study to detect multiple respiratory viruses simultaneously, offering a potentially more sensitive and efficient approach compared to traditional methods.[7] (3) A PCR method allowing simultaneous amplification of multiple targets in a single reaction.[8]
From: International Journal of Environmental Research and Public Health (MDPI)
(1) Multiplex polymerase chain reaction, or PCR, is a molecular technique used to detect the presence of specific genes related to antibiotic resistance and virulence.[9] (2) Multiplex polymerase chain reaction can be used to detect virulence factors of Escherichia coli from children, as referenced in the provided information.[10]