Significance of Multiplex PCR
Multiplex PCR is a molecular biology technique and a variation of the polymerase chain reaction. It enables the simultaneous detection and amplification of multiple DNA targets in a single reaction. This method is used for various diagnostic purposes, including identifying pathogens, detecting antibiotic resistance genes, and analyzing genetic sequences from mixed samples. Multiplex PCR enhances the efficiency and specificity of detection, allowing for the identification of multiple strains or organisms, such as in the diagnosis of diseases in humans and animals.
Synonyms: Multiplex polymerase chain reaction, Multiplex pcr assay, Multiplex amplification
The below excerpts are indicatory and do represent direct quotations or translations. It is your responsibility to fact check each reference.
The concept of Multiplex PCR in scientific sources
Multiplex PCR is a molecular technique that amplifies multiple DNA targets simultaneously in a single reaction. This method enhances detection efficiency, allowing for the identification of various pathogens, genes, or organisms, like bacteria and viruses, in a single sample. It is used in diagnostics and research.
From: Onderstepoort Journal of Veterinary Research
(1) This method is used to target RD 4 and RD 9 genomic regions for deletion analysis.[1] (2) This is a molecular method used for diagnosing specific organisms in small ruminants, allowing for the detection of multiple organisms simultaneously.[2] (3) This is a type of polymerase chain reaction used in the study to target multiple virulence factors of Escherichia coli simultaneously.[3] (4) A variation of the PCR technique that allows the detection of multiple strains or pathogens in a single sample simultaneously.[4] (5) The term "multiplex PCR" is mentioned in the context of differentiating bacterial species, implying the use of a specific laboratory technique for identifying and studying different types of bacteria.[5]
From: The Malaysian Journal of Medical Sciences
(1) This refers to a technique used in molecular biology, used for detecting and analyzing multiple genetic sequences in a single reaction.[6] (2) This is a PCR method that amplifies multiple DNA targets simultaneously, and the study developed a multiplex method for detecting CYP3A4 alleles.[7] (3) A PCR method that allows multiple target DNA regions to be amplified simultaneously in a single reaction using multiple primer sets.[8] (4) An advanced form of polymerase chain reaction that allows for simultaneous amplification of multiple target genes, enhancing the sensitivity and specificity for detecting Mycobacterium tuberculosis.[9] (5) The multiplex PCR (mPCR) assay developed in this study detects both the 16S rRNA gene and the major outer membrane lipoprotein gene (LipL32) for detecting Leptospira's DNA.[10]
From: International Journal of Pharmacology
(1) Multiplex PCR is a technique used to amplify multiple DNA targets simultaneously, facilitating high-resolution genotyping of regions with high homology, like those in CYP2D6 alleles.[11]
From: International Journal of Environmental Research and Public Health (MDPI)
(1) Multiplex PCR was used for detection of genes encoding for tetracycline resistance, ESBL, metallo beta-lactamases, and virulence genes.[12] (2) This is a method used to amplify and identify multiple genes simultaneously, confirming the presence of E. coli.[13] (3) Multiplex PCR is used to demonstrate preferential binding of SYBR Green I to specific DNA fragments, enhancing the accuracy and sensitivity of detection.[14] (4) Multiplex PCR is used for rapid detection of shiga toxin-producing bacteria in feces with molecular beacons, as evidenced in the provided text.[15] (5) This is a variation of a molecular biology technique that allows for the simultaneous detection of multiple bacterial species in a sample, improving efficiency in safety control surveys.[16]
From: International Journal of Pharmacology
(1) This is a molecular biology technique that allows for the simultaneous detection of multiple DNA targets, such as identifying Staphylococcus aureus and antibiotic resistance genes.[17] (2) A polymerase chain reaction technique that amplifies multiple DNA targets simultaneously, used here to detect the mcr-1 gene.[18] (3) Multiplex PCR is a diagnostic tool used with subgingival plaque samples for the effective management and diagnosis of periodontal disease, identifying specific pathogens.[19]