Significance of MTT assay

The MTT assay is a colorimetric method used to assess cell viability and cytotoxicity by measuring the metabolic activity of living cells. It operates on the principle that viable cells can reduce the yellow MTT salt to purple formazan crystals, indicating their presence and health. This assay is frequently employed in various studies, including cancer research, to evaluate the effectiveness of treatments on different cell lines, providing a quantitative measure of cell proliferation and metabolic activity.

Synonyms: Cell viability assay, Cell proliferation assay, Colorimetric assay

The below excerpts are indicatory and do represent direct quotations or translations. It is your responsibility to fact check each reference.

Hindu concept of 'MTT assay'

Hinduism Books

MTT assay in Hinduism interprets laboratory tests assessing cytotoxic effects of substances like Rajat Sindoor and Rajata Bhasma, highlighting their anticancer potential by measuring cell viability and metabolic activity, relevant in traditional medicinal research.

Significance in Ayurveda (science of life):
Ayurveda Books

(1) This is a laboratory test used to assess the cytotoxic effects of a substance, such as Rajat Sindoor, by measuring the metabolic activity of cells and determining their viability.[1] (2) This is a test used in research to measure cell viability and assess the anticancer potential of various substances, including Calotropis procera extracts.[2] (3) MTT assay reveals the potent cytotoxic activity of Rajata Bhasma against MCF-7 cell lines and is used to study its effects.[3]

Ayurveda is a branch of Indian science dealing with medicine, herbalism, taxology, anatomy, surgery, alchemy and related topics. Traditional practice of Ayurveda in ancient India dates back to at least the first millenium BC. Literature is commonly written in Sanskrit using various poetic metres.

The concept of MTT assay in scientific sources

Science Books

The MTT assay is a colorimetric assay that evaluates cell viability by measuring the reduction of MTT to formazan. It is used to assess metabolic activity and cytotoxic effects, particularly against cancer cells like PC-3.

Significance in Scientific journals, articles, etc.:

(1) This is a method that was used to determine cell viability, and it involves the reduction of a tetrazolium salt.[4] (2) This is a specific method that was used to assess the cytotoxicity of goniothalamin in the cells, in order to measure its effects.[5] (3) This is a biochemical assay used to measure the cytotoxic activity of the extracts and compounds against the specified cell lines.[6] (4) MTT assay is a method used to measure cell viability and assess the cytotoxicity of the extracts on cell lines.[7] (5) A laboratory test used to determine the cytotoxicity of tamarillo extracts on cancer cell lines by measuring the reduction of MTT to formazan.[8]

This section includes scientific research in the field of healthcare, often involving traditional medicine systems such as Ayurveda, Unani, Siddha and Holistic health. It includes papers from scientific journals classified as either original, clinical or experimental research, review articles or case reports.