Significance of Limit of detection
The limit of detection (LOD) refers to the lowest concentration of a substance that can be reliably detected using an analytical method, although not necessarily quantified. This key concept is critical in determining method sensitivity and is defined for various substances. In specific studies, LOD values were noted for different drugs, such as amoxicillin at 2.5 µg/kg and ciprofloxacin at 61.2 µg/ml. Ultimately, LOD provides essential information on the analytical capability of methods under investigation.
Synonyms: Detection threshold, Sensitivity limit, Minimum detectable level, Detection limit, Threshold, Sensitivity, Analytical sensitivity
The below excerpts are indicatory and do represent direct quotations or translations. It is your responsibility to fact check each reference.
The concept of Limit of detection in scientific sources
The Limit of Detection (LOD) is the minimal concentration of a substance that can be reliably detected by an analytical method, indicating the method's sensitivity; however, it does not imply quantification of that substance.
From: Asian Journal of Pharmaceutics
(1) The LOD was found to be 0.0279 μg/mL, it is the lowest concentration of analyte that can be detected.[1] (2) A measurement that was taken during the experiment using the novel bienzymatic assay method.[2] (3) The values were 0.6287, 0.1598, and 0.1568 µ g/mL for tenofovir disoproxil fumarate, dolutegravir sodium, and emtricitabine.[3] (4) The lowest concentration of empagliflozin that can be detected, determined based on the calibration plot.[4] (5) Limit of detection (LOD) is calculated by evaluating signal to noise ratio, i.e., 3.3, using equations mentioned in ICH Q 2 (R 1), 2005.[5]
From: International Journal of Pharmacology
(1) This was 100 ng/mL for the HPLC method used to analyze gemfibrozil.[6] (2) This was at 0.0025 mg/mL, the limit of quantitation at 0.01 mg/mL was established during the study.[7] (3) The lowest concentration of enhydrin and uvedalin that can be detected, determined to be 2.913 and 38.25 μg mL –1, respectively.[8] (4) It is defined with a S/N ratio 5:1 in serum.[9] (5) This and Limit of Quantification were established at a signal to noise ratio (S/N) of 3.3 and 10.[10]
From: Onderstepoort Journal of Veterinary Research
(1) The smallest amount of an analyte in a sample that can be reliably detected by an analytical method, used to assess the sensitivity of the method.[11] (2) This represents the lowest concentration of isometamidium that the ELISA could reliably detect, as described in the text.[12] (3) This is the lowest concentration of oxalates that can be reliably detected by the method, determined using a specific formula.[13] (4) This is the lowest concentration that the method is able to detect, which is determined using regression statistics from the research data.[14]
From: The Malaysian Journal of Medical Sciences
(1) The limit of detection for propranolol, the lowest concentration detectable by the HPLC method, ensured the sensitivity of the analysis and the reliability of the results.[15]
From: Journal of Public Health in Africa
(1) The minimum concentration of parasites in a blood sample, approximately two per microliter, required to confidently declare that the microscopic slide examination has confirmed a positive result.[16]
From: South African Journal of HIV Medicine
(1) The limit of detection for the CAP/CTM v2.0 assay is reported to be 300 RNA copies/mL, which is an improvement from the previous version's limit of detection of 1090 RNA copies/mL using 60 μL DBS specimens.[17]
From: International Journal of Environmental Research and Public Health (MDPI)
(1) A quantitative measure established for the developed method, indicating the lowest concentration of the target substance that can be reliably distinguished from a blank sample, determined to be a very low value in nanograms per milliliter.[18] (2) The limit of detection (LOD) for all investigated analytes was established at 0.01 µ g/L serum, representing the minimum concentration that could be reliably distinguished from background noise in the analytical measurements.[19] (3) Limit of Detection (LOD) is defined as the minimum analyte level detected in a sample and was estimated to be one-half the limit of quantification (LOQ).[20] (4) This can be improved up to 10-fold using EuNP immunochromatography compared with the traditional colloidal gold-based immunochromatography method, showcasing the enhanced sensitivity.[21] (5) Limit of detection, also known as LOD, is a measure not explicitly defined in the text, but generally refers to the lowest quantity of a substance that can be distinguished from the absence of that substance within a stated confidence limit.[22]
From: Sustainability Journal (MDPI)
(1) The limit of detection, set at 10 µg/L for molybdenum, was uniformly reached by the plasma concentration of Mo in sheep until they finished their time grazing within the treated vineyard areas.[23] (2) It was estimated using a 10-fold 1:5 serial dilution with artificial frass obtained by adding frass from another species to RLB DNA larvae.[24] (3) It is the lowest quantity of a substance that can be detected with a stated confidence, though not necessarily quantified, under the experimental conditions of the method.[25] (4) This is the lowest quantity of a substance that can be reliably detected by an analytical method, indicating the sensitivity of the measurement.[26] (5) The lowest concentration of mercury that can be reliably detected.[27]
From: International Journal of Pharmacology
(1) The lowest concentration of an analyte that can be reliably detected, typically defined by a signal-to-noise ratio.[28] (2) This is the lowest concentration of warfarin that can be reliably distinguished from background noise.[29]