Significance of Isocratic elution
Isocratic elution is a chromatographic technique where the mobile phase composition remains constant throughout the separation process. This method utilizes a single solvent or a mixture of solvents consistently during the analysis. It is particularly valued for its simplicity and reproducibility, making it useful in various applications, such as the determination of compounds like Neostigmine. Overall, isocratic elution is essential for achieving reliable separation results in high-performance liquid chromatography (HPLC) and other chromatography methods.
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The concept of Isocratic elution in scientific sources
Isocratic elution is a chromatographic technique where the mobile phase composition remains constant, facilitating the separation of compounds in a study's extract by ensuring uniform solvent composition throughout the process.
From: World Journal of Pharmaceutical Research
(1) A chromatographic technique where the mobile phase composition remains constant throughout the separation process.[1] (2) A chromatographic technique where the solvent composition remains constant, used in the separation of components in the study.[2] (3) A technique used in the RP-HPLC method where a single solvent or mixture is used throughout the separation process.[3] (4) A chromatography technique where the mobile phase composition remains constant throughout the separation process.[4] (5) A type of elution in HPLC where the mobile phase composition remains constant throughout the separation process.[5]
From: AYU (Journal of Research in Ayurveda)
(1) A chromatographic technique where the composition of the mobile phase remains constant throughout the separation process.[6]