Significance of In vitro assay
In vitro assays, as defined across various fields, are laboratory tests conducted outside of a living organism. These tests, performed in controlled environments, are used to study biological processes, assess the effects of substances, and evaluate the efficacy of treatments. They are crucial for studying antioxidant capacity, antibacterial potential, anti-inflammatory activity, and other biological effects. In vitro assays often involve testing on cells or biological molecules in test tubes or culture dishes.
Synonyms: Laboratory test, Experimental procedure, Cell culture assay, Biological assay, In vitro test, Experimental assay, In vitro evaluation, Bench test, In vitro experimentation
The below excerpts are indicatory and do represent direct quotations or translations. It is your responsibility to fact check each reference.
Hindu concept of 'In vitro assay'
In Hinduism, an "in vitro assay" appears as a method to study plant extracts. It involves testing the impact of alcoholic extracts from seeds and leaves on microfilariae. Additionally, it is used to evaluate the antioxidant properties of carpet weed.
From: Journal of Ayurvedic and Herbal Medicine
(1) These are systems used to determine the free radical scavenging and antioxidant activities of Glinus oppositifolius (carpet weed), as used in a study.[1]
From: International Research Journal of Ayurveda and Yoga
(1) Alcoholic extracts of both seeds and leaves killed microfilariae in an in-vitro assay.[2]
The concept of In vitro assay in scientific sources
In vitro assays are laboratory tests conducted outside a living organism in a controlled environment, such as test tubes or culture dishes. These tests evaluate biological activity, the efficacy of treatments, and the properties of various substances on cells, samples, or biological molecules.
From: International Journal of Pharmacology
(1) Experimental procedures used to analyze the antioxidant and anti hyperglycemic effects of BDMCA.[3] (2) The bio-inspired syntheses of silver nanoparticles using aqueous piper longum fruit extract have revealed potential antimicrobial properties in these.[4] (3) Assays use various end points like MTT, ATP, DNA strand breaks, etc., for measuring cell kill.[5] (4) The genotoxic effects of estrogens/synthetic progestins/androgens have been demonstrated in various in vitro assays, using cultured animal cells or cell free systems.[6] (5) These assays involve cultures of isolated endothelial cells that are useful to study the formation of micro vessels in a controlled environment.[7]
From: Asian Journal of Pharmaceutics
(1) These are tests performed outside of a living organism to analyze antiurolithiatic effects.[8] (2) Experiments conducted outside of a living organism, often used to assess cell viability and proliferation in response to external factors.[9] (3) Regarding the mechanism of action for PCs in regenerative implantology, it is becoming crucial to discuss the GF content in the platelet-derived products when evaluated by these in the laboratory.[10] (4) This assay is used to evaluate chromatin degradation by candidate apoptotic nucleases.[11] (5) This research was conducted to evaluate the nanoparticle ethanol extract of cloves.[12]
From: Onderstepoort Journal of Veterinary Research
(1) These are tests used to screen molasses batches for potential endocrine-disrupting effects, which helped in selecting the batches for the calf feeding trial.[13] (2) Laboratory tests used to assess the effectiveness of stabilates, such as titration, which can be used to predict the performance of vaccines in vivo.[14] (3) These are tests performed in the laboratory to assess the effects of geigerin, which indicated that necrosis was not observed in the C2C12 myoblasts following exposure.[15]
From: South African Journal of HIV Medicine
(1) This is a laboratory test conducted outside of a living organism, specifically used to study the effects of salivary mucins on HIV-1 subtypes A and C.[16]
From: International Journal of Environmental Research and Public Health (MDPI)
(1) The study was the first to assess the effect of Oxadiazon on estrogen receptors using an in vitro assay, which is a novel aspect of the research.[17] (2) While the CSPA framework relies on curated databases for toxicological assessment, ToxPi uses high throughput data from the in vitro assays publicly available through the ToxCast database.[18] (3) In vitro assays are tests conducted outside of a living organism, often using cells or tissues in a controlled environment, and these assays can be utilized to screen massive environmental pollutants, as mentioned.[19] (4) In vitro assays are used in the comparative analysis of estrogenic activity in sewage treatment plant effluents.[20] (5) Therefore, considering the uncertainties amongst in vitro assays and in vivo models, a IVIVR is mandatory prerequisites for scientific acceptance before these in vitro assays to be used as a surrogate measurement.[21]
From: International Journal of Pharmacology
(1) This is a method used to evaluate the potential of drugs or medicinal plants to inhibit CYP-mediated metabolism and minimize herb-drug interactions, as described.[22]
From: International Journal of Pharmacology
(1) In vitro assays are utilized to evaluate the Anti-Cryptosporidium parvum activity of substances like Artemisia judaica L. and its fractions.[23] (2) Laboratory tests conducted outside of a living organism, typically in test tubes or culture dishes, to study biological processes.[24] (3) Laboratory tests conducted in controlled environments, outside of a living organism, used here to assess the antibacterial potential of plant extracts.[25] (4) In-vitro assays are laboratory tests performed outside a living organism, typically in a controlled environment like a test tube or petri dish, to study biological processes.[26] (5) In vitro assays are experimental methods used to evaluate the antioxidant capacity of extracts, such as superoxide radical scavenging and DPPH radical scavenging activities.[27]