Significance of HPLC Technique
High-Performance Liquid Chromatography (HPLC) is an analytical technique widely employed for the separation, identification, and quantification of compounds in various mixtures. This method is particularly valuable in analyzing secondary metabolites, checking the purity of synthesized compounds, and evaluating phenolic and flavonoid profiles in plants. Additionally, HPLC is utilized in pharmaceutical applications, such as determining escitalopram and Ivermectin levels in preparations. Its precision makes it essential for quality control and research in various scientific fields.
Synonyms: High-performance liquid chromatography, Liquid chromatography, Hplc, Column chromatography, High-pressure liquid chromatography, Hplc method, Hplc analysis
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The concept of HPLC Technique in scientific sources
HPLC Technique, or High-Performance Liquid Chromatography, is an analytical method vital for assessing the purity, separation, and quantification of compounds, including plant-derived substances like quercetin, ensuring quality in synthesized compounds.
From: Asian Journal of Pharmaceutics
(1) These techniques were used for the qualitative and quantitative analysis of aflatoxins.[1] (2) A major analytical technique used worldwide, with simplicity and efficiency.[2] (3) A method that detected efficiently AFB 1-producing isolates in 10 (66.7%) out of 15 isolates of A. flavus with rates ranged from 0.78 to 45.03 ppm.[3]
From: International Journal of Environmental Research and Public Health (MDPI)
(1) It is a method used to determine the presence of carbaryl and its degradation product, 1-naphthol, in plant and nutrient solution samples.[4]